CONTAVECT/PYDNA DEVELOPMENT NOTEBOOK

THIS NOTEBOOK WAS INITIALLY USED TO FOLLOW THE DEVELOPMENT OF ISFINDER.
ISFINDER DEVELOPMENT WAS DISCONTINUED BUT MOST OF ITS CODE WAS REUSED IN CONTAVECT AND pyDNA

2014 05 20

Input Data

Paired fastq.gz file (an artificial dataset can be generated using ISIS
Reference genome of virus and host DNA in fasta format
Annotation files of host reference genome to locate features such as genes, CpG, active chromatine... (Bed format?)
Configuration file containing all algorithm parameters
output name use as a prefix for output data

Required Output (for biologists...)

After a de novo reconstruction of junctions from chimeric pairs and reads a report containing the characteristics of each junction and the number of reads in support of its exxistence will be generated
To visualize junctions a fasta reference of reconstructed will be generated and BAM/BAI/bedGraph file containing supported reads will also be generated
In addition, BAM, BAM index and BedGraph for the following categories of sequences
- Unmapped sequences (BAM only)
- Both mate of a pair in virus genome
- Both mate of a pair in host genome
- Pairs ovelapping true junctions
- Pairs corresponding to NGS prep artifacts (not a true junction)
A circos plot of insertion distribution in host genome will be created with Circos
Using host genome annotation feature, the enrichment of junction nears specific feature will be summurize in a report (CpG, Gene, heterochromatin...)

Specificity of the analysis

Extract virus/host genome junctions in pair end sequencing data : Junction could be inside a read (chimeric read) or between the 2 mate of a pair (chimeric pairs)
Bad quality sequences may result in unmapped reads or false positive
A large number of sequence may result in extensive calculation time
Homology between virus and fragment of host genome may result in false positive junctions.
Filter out false junctions created during NGS library preparation

2014 05 21

Initial design proposed for IsFinder

Exploration of Blast integration in biopython = Drafting function for CoordHits

NCBIWWW module = BLAST over the Internet



In [ ]:

    
from Bio.Blast.NCBIWWW import qblast
qblast('blastn', 'nr', 'ATATCCGTAGTATTTATATGCGCGTAGCTCGCTGCTTATTATCGCTGATCTC')
# Dos not work in the lab due to the proxy server...

In all cases this online requesting tool is not suitable for IsFind pipeline. Blast need to be run locally with a local dadabase consisting only of host genomic DNA. For this we can use the NCBI BLASTX wrapper from the Bio.Blast.Application



In [ ]:

    
from Bio.Blast.Applications import NcbiblastxCommandline as blast
help(blast)

A blast database had to be prepared before using blast

Test of makeblast in bash shell.

call or check call had to be used to launch command line in the shell check call is better since it raise a CalledProcessError in case of failure



In [ ]:

    
# command line with an error
from subprocess import check_call
check_call(("makeblastdb -in multi.fa -dbtype nucl -input_type fast"), shell=True)



In [ ]:

    
# command line without error
check_call(("makeblastdb -in multi.fa -dbtype nucl -input_type fasta"), shell=True)

Command line execution function



In [ ]:

    
from subprocess import check_call, CalledProcessError

def command_bool(command):
    """ Generic function to execute command lines return true or false
    """
    try:
        print (command)
        check_call((command), shell=True)
        print ("Success")
    except (OSError, CalledProcessError) as E:
        print (E)
        exit (0)

#Definition of the reference and construction of the command text line
reference = "multi.fa"
command = "makeblastdb -in {0} -dbtype nucl -input_type fasta".format(reference)

# Call of command_line
command_bool(command)

Blastn using biopython parser = not much informative and can be easily done without



In [ ]:

    
from Bio.Blast.Applications import NcbiblastnCommandline as blastn

command = blastn(query="frag.fa", db="multi.fa", evalue=0.001, outfmt=1, out="test.xml")
print (command)

stdout, stderr = command()
print (stdout)

Using my command_bool functions



In [ ]:

    
query = "frag.fa"
db = "multi.fa"
command = "blastn -outfmt '6 sseqid sstart send' -query {0} -db {1} -evalue 0.001".format(query, db)

# Call of command_line
print (command_bool(command))

Call or check_call are not able to return the command line return value. Instead Popen should be used for this



In [ ]:

    
from subprocess import Popen, PIPE

def command_return(command):
    """ Generic function to execute command lines return true or false
    """
    try:
        print (command)
        return Popen(command, shell=True, stdout=PIPE).stdout.read()
    except (OSError, ValueError) as E:
        print (E)
        exit (0)



In [ ]:

    
query = "frag.fa"
db = "multi.fa"
command = "blastn -outfmt '6 sseqid sstart send' -query {0} -db {1} -evalue 0.001".format(query, db)

# Call of command_line
command_return(command)

It work and return a list of string with each string composed of the subject ref name followed by the start and end of the blast hit. Now Let's build fonctions to parse this results and store it in a dictionnary.

2014 05 22

Split lines from a raw blast CSV output



In [ ]:

    
def split_blast_lines (command):
    return [i for i in command_return(command).splitlines()]
        
split_blast("blastn -outfmt '6 sseqid sstart send' -query frag.fa -db multi.fa -evalue 0.001")

List Version of blast hits results



In [ ]:

    
def list_blast (command):
    return [i.split("\t") for i in split_blast_lines (command)]

list_blast("blastn -outfmt '6 sseqid sstart send' -query frag.fa -db multi.fa -evalue 0.001")

Dict Version of blast hits results (with additional str to int conversion of start and end)



In [ ]:

    
def dict_blast (command):
    d = {}
    for ref, start, end in list_blast(command):
        if ref in d:
            d[ref].append([int(start), int(end)])
        else:
            d[ref] = [[int(start), int(end)]]
    return d

dict_blast("blastn -outfmt '6 sseqid sstart send' -query frag.fa -db multi.fa -evalue 0.001")

Summary of functions for CoordHits



In [ ]:

    
from subprocess import Popen, PIPE

def command_return(command):
    try:
        return (Popen(command, shell=True, stdout=PIPE).stdout.read())
    except (OSError, ValueError) as E:
        print (E)
        exit (0)    

def split_blast_lines (command):
    return [i for i in command_return(command).splitlines()]

def list_blast (command):
    list_blast = [i.split("\t") for i in split_blast_lines (command)]
    print ("{0} hit(s) found".format(len(list_blast)))
    return list_blast

def dict_blast (command):
    d = {}
    for ref, pos1, pos2 in list_blast(command):
        
        # Cast positions in integers
        pos1 = int(pos1)
        pos2 = int(pos2)
        
        # Return end and start in the appropriate order
        start = pos1 if pos1 <= pos2 else pos2
        end = pos2 if pos1 <= pos2 else pos1
        
        # Fill the dictionary iteratively
        if ref in d:
            d[ref].append([start, end])
        else:
            d[ref] = [[start, end]]
    return d



In [ ]:

    
#Definition of the reference and construction of the command text line
query = "../data/AAV_RSV_GFP.fa"
subject = "../data/mm10.fa"
cmd = "makeblastdb -in {0} -dbtype nucl -input_type fasta".format(subject)

print ("Creating subject database") 
print(command_return(cmd))

print ("Blast {0} against {1} database".format(query, subject))
cmd = "blastn -outfmt '6 sseqid sstart send' -query {0} -db {1} -evalue 0.1".format(query, subject)
dict_blast(cmd)

It works with the whole mouse genome versus an AAV genome. It is really fast even taking into account database building (> 1 min). However Blast option needs to be optimize since only few hits are found only in chr12. The blast User Manuel may help to determine better parameters USER MANUAL

blastn algorithm seems more adapted than the default option megablast to find partial matches, but it is also a little slower. Parameters were change to increase the number of hits:

-evalue was remove to reach its default value = 10
-dust was deactivated to avoid query filtering
-num_threads was added to use parralel CPU processing.



In [ ]:

    
from multiprocessing import cpu_count

def blastn_hits (query, subject, evalue=10):
    
    cmd = "makeblastdb -in {0} -dbtype nucl -input_type fasta".format(subject)
    print ("Creating subject database") 
    print(command_return(cmd))
    
    cmd = "blastn -task blastn -outfmt '6 sseqid sstart send' -dust no -num_threads {0} -evalue {1} -query {2} -db {3}".format(
        cpu_count(),
        evalue,
        query,
        subject)
    
    print ("Blast query against subject database")
    return (dict_blast(cmd))



In [ ]:

    
blastn_hits ("../data/AAV_RSV_GFP.fa", "../data/mm10.fa")

These parameters sound suitable for a future implementation of HitCoord class

Using hit coordinates to clean host ref from Blast results Draft of Ref Curator class



In [ ]:

    
from Bio import SeqIO

# FUNCTION FROM ISIS CLASS REFERENCEGENOME
def import_fasta(filename):
    """Import fasta files in a dictionary of biopython SeqRecord
    """
    # Try to open the file fist gz compressed and uncompressed
    try: 
        if filename.rpartition(".")[-1] == "gz":
            print("\tUncompressing and extracting data")
            handle = gzip.open(filename, "r")
        else:
            print("\tExtracting data")
            handle = open(filename, "r")

        d = SeqIO.to_dict(SeqIO.parse(handle, "fasta"))
        handle.close()
        
        return d
        
    # Try to open the file fist gz compressed and uncompressed
    except IOError:
           print('CRITICAL ERROR. The fasta file ' + filename + ' is not readable. Exit')
           exit

def curate_ref (query, subject, evalue):
    
    print ("Searching for virus homologies in host genome")
    hits = blastn_hits(query, subject, evalue)
    print (hits)
    
    if not hits:
        print ("No hits found. The original host genome sequence will not be edited")
    else :
        print ("\nImporting host genome for hard masking of homologies")
        genome = import_fasta(subject)
        
        # For all reference in which a blast hit was found 
        for ref, coord_list in hits.items():
            # Transform the reference sequence to modify in a mutable sequence
            print ("Editing reference sequence : {}".format(ref))
            genome[ref].seq = genome[ref].seq.tomutable()
            # For all blast hit coord found in the current reference
            for start, end in coord_list:
                # For all position between start en end coordinates modify the base by N
                for position in range (start, end):
                    genome[ref].seq[position] = 'N'
        
        # Write the new reference in fasta format
        genome_basename = subject.rpartition('/')[2].partition('.')[0]
        print ("Writing new version of {} in which homology with the virus are masked".format(genome_basename))
        with open(genome_basename + "_masked.fa", 'w') as f:
            for ref in genome.values():
                f.write(ref.format("fasta"))
    print ("DONE")



In [ ]:

    
import time
start_time = time.time()

curate_ref("../data/frag.fa", "../data/multi.fa", 10)

print "\nExecution time {} seconds".format(time.time() - start_time)

It works pretty fast with a short sequence and subject. Now let's try it with the whole mouse genome vs an rAAV vector



In [ ]:

    
import time
start_time = time.time()

curate_ref("../data/AAV_RSV_GFP.fa", "../data/mm10.fa", 10)

print "\nExecution time {} seconds".format(time.time() - start_time)

That is really impressive taking into account that the mouse genome blast index was build, the mouse genome was imported, modified and rewritten...

2013 05 23

Now that all function for Ref curator and Blastn are available and functional, I will transpose them in a object oriented model. There will be several modifications to the original organization.

First blast hit will not been returned as a dictionnary of hits but as hit.objects tracked in a class list : Here is a preliminary code for this class



In [ ]:

    
####################################################################################################

class BlastHit(object):
    """Class description
    """
####################################################################################################
    
####    CLASS FIELD    ####
    
    Instances = [] # Class field used for instance tracking 
    
####    CLASS METHODS    ####
    
    @ classmethod
    def count_total (self):
        return (len(self.Instances))
    
    @ classmethod
    def count_per_ref (self):
        d = {}
        for hit in Instances:
            if hit.s_id in d:
                d[ref] +=1
            else:
                d[ref] = 0
        return d
        
    @ classmethod
    def get (self):
        return Instances
        
    @ classmethod
    def getref (self, ref):
        return [hit for hit in Instances if hit.s_id == "ref"]
        
####    FONDAMENTAL METHODS     ####
   
    def __init__(self, q_id,s_id, identity, length, mis, gap, q_start, q_end, s_start, s_end, evalue, bscore):
        """Object constructor"""
        
        # Name of the reference seq in the query and in the subject were a hit was found
        self.q_id = q_id
        self.s_id = s_id
        # Percentage of identity
        self.identity = float(identity)
        # Length of the alignement
        self.length = int(length)
        # Number of mismatches and gaps
        self.mis = int(mis)
        self.gap = int(gap)
        # Start and end position along the query sequence
        self.q_orient = int(q_start) < int(q_end) # True if orientation is positive
        self.q_start = int(q_start) if self.q_orient else int(q_end)
        self.q_end = int(q_end) if self.q_orient else int(q_start
        # Start and end position along the subject sequence
        self.s_orient = int(s_start) < int(s_end) # True if orientation is positive
        self.s_start = int(s_start) if self.s_orient else int(s_end)
        self.s_end = int(s_end) if self.s_orient else int(s_start
        # Evalue and bit score of the match
        self.evalue = float(evalue)
        self.bscore = int(bscore)
        # Add the instance to the class instance tracking list
        self.Instances.append(self)
    
    def __repr__(self):
        """Long representation"""
        msg = "{}\n".format(self.__str__())
        msg += "\tQuery\t{}:{}-{}({})\n".format(self.q_id, self.q_start, self.s_end, self.q_orient)
        msg += "\tSubject\t{}:{}-{}({})\n".format(self.q_id, self.q_start, self.s_end, self.q_orient)
        msg += "\tLenght : {}\tIdentity : {}%\tEvalue : {}\tBit score : {}\t".format(self.length, self.identity, self.evalue, self.bscore)
        
    def __str__(self):
        """Short representation"""
        return "<Instance of " + self.__module__ + ">"

2014 05 26

@ Blast Module

The entire Module is working properly. It consists of 2 classes :

Blast is a singleton class that contains functions to execute makeblastdb (blast database creation) and blastn.
BlastHit is instancied for each blast hit found by do_blast() from Blast class (see above).

in order to reuse generic utilities in future classes, I created a utilities module in which I put run_shell() and mkdir() functions. More function should be added during the developpement.

2014 05 27

General

Introduction of doxygen documentation synthax to allow a simple and complete auto-generation of the documentation Doxygen

Doxygen can be easily integrated in python docstring using doxypy.

The synthax is really simple and text bloc can be formated using markdown :

@bref : Brief description
@author : List of authors
@param : Description of each parameters
@return : Description of the return value
@exception : Description of possible exceptions raised by the function
...

Complete implementation of doxigen documentation in all functions, classes and package.

Restructuration of the initial classes = grouping Blast, BlastHit and RefCurator (now called RefMasker) in a single package (same file) = HomologyMasker A masker() function was writen in RefMasker in order to be able to blast multiple query references against the reference database in a row. This function was not really critical for Isfinder, but it is necessary for the future analysis pipeline another of my NGS project.

See an exemple with a small dataset bellow



In [ ]:

    
from HomologyMasker import RefMasker
RefMasker.masker(["../test/q1.fa", "../test/q2.fa", "../test/q3.fa"], "../test/s1.fa", 0.0000001)

TODO

Find a strategy to trim adapters and quality
Start drafting of fastqTrimmer and fastqAligner

2014 06 16

Draft fastq import and qualFilter



In [39]:

    
from Bio import SeqIO
import gzip

print("Uncompressing and extracting data")
R1 = gzip.open("../test/fastq/small.R1.fastq.gz", "r")
R2 = gzip.open("../test/fastq/small.R2.fastq.gz", "r")

genR1 = SeqIO.parse(R1, "fastq")
genR2 = SeqIO.parse(R2, "fastq")

ListR1 = []
ListR2 = []

while True:
    seqR1 = next(genR1, None)
    seqR2 = next(genR2, None)
    
    if not seqR1 or not seqR2:
        print ("End of list")
        break
    
    # Compute mean quality score
    qMeanR1 = float(sum(seqR1.letter_annotations['phred_quality']))/len(seqR1)
    qMeanR2 = float(sum(seqR2.letter_annotations['phred_quality']))/len(seqR2)
    # Filter read pair based on the mean quality
    if qMeanR1 >= 32 and qMeanR2 >= 32:
        ListR1.append(seqR1)
        ListR2.append(seqR2)
        
print (len(ListR1))

R1.close()
R2.close()









    



Uncompressing and extracting data
End of list
16

Fastq import and quality score is working properly

Draft primer trimmer



In [40]:

    
import re

#~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~#

def find_matches(sequence, primer_list):
    
    match_list = []

    for primer in primer_list:
        match_gen = re.finditer(primer, sequence, re.I)
        for match in match_gen:
            match_list.append([match.start(), match.end()])
    return (match_list)

#~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~#

def longer_interval(match_list, maxsize):
    
    start_max = 0
    end_max = 0
    inter_max = 0
    end_prec = -1
    
    match_list.sort()
    match_list.append([maxsize, 0])
    print (match_list)
    
    for start, end in match_list:
        if end > end_prec:
            if start - end_prec - 1 > inter_max:
                start_max = end_prec
                end_max = start
                inter_max = start - end_prec - 1
            end_prec = end 
    print ("Longer interval = {} [{}:{}]".format(inter_max, start_max+1, end_max-1))
    return (start_max+1, end_max-1)
 
#~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~#

PRIMER_LIST = ["gcgcg", "CGcC"]
MIN_SIZE = 50

for mate, fastq_list  in [["R1", ListR1], ["R2", ListR2]]:
    print ("\nORIENTATION " + mate + "\n")
  
    for i in range(len(fastq_list)):
        print i
        match_list = find_matches(str(fastq_list[i].seq), PRIMER_LIST)
        
        if match_list:
            print ("{} hit(s) found".format(len(match_list)))
            start, end = longer_interval (match_list, len(fastq_list[i])) 
            if end - start < MIN_SIZE:
                print ("Trimming resulted in short sequence. Pair removed") 
                ListR1.pop(i)
                ListR2.pop(i)
            else:
                fastq_list[i] = fastq_list[i][start:end]
        
    for fastq in ListR1:
        print ("{} : {}\n".format(fastq.id, fastq.seq))









    



---------------------------------------------------------------------------
IndexError                                Traceback (most recent call last)
<ipython-input-40-4f868981f8b5> in <module>()
     46     for i in range(len(fastq_list)):
     47         print i
---> 48         match_list = find_matches(str(fastq_list[i].seq), PRIMER_LIST)
     49 
     50         if match_list:

IndexError: list index out of range





    



ORIENTATION R1

0
1 hit(s) found
[[145, 150], [150, 0]]
Longer interval = 145 [0:144]
1
1 hit(s) found
[[128, 133], [150, 0]]
Longer interval = 128 [0:127]
2
1 hit(s) found
[[114, 119], [150, 0]]
Longer interval = 114 [0:113]
3
4
3 hit(s) found
[[1, 6], [62, 67], [113, 117], [150, 0]]
Longer interval = 55 [7:61]
5
6
7
1 hit(s) found
[[92, 97], [150, 0]]
Longer interval = 92 [0:91]
8
3 hit(s) found
[[52, 57], [103, 107], [143, 147], [150, 0]]
Longer interval = 52 [0:51]
9
10
3 hit(s) found
[[2, 6], [42, 46], [100, 105], [150, 0]]
Longer interval = 53 [47:99]
11
3 hit(s) found
[[63, 67], [102, 106], [138, 142], [150, 0]]
Longer interval = 63 [0:62]
12
1 hit(s) found
[[79, 84], [150, 0]]
Longer interval = 79 [0:78]
13
1 hit(s) found
[[73, 77], [150, 0]]
Longer interval = 73 [0:72]
14
1 hit(s) found
[[9, 13], [150, 0]]
Longer interval = 9 [0:8]
Trimming resulted in short sequence. Pair removed
15

2014 06 17

After investigation the way adapter cutting is usually done in other NGS quality/primer trimmer is done, It seems that it is usually more complicated than the way used in my draft. Imperfect matches are often taken into account as well as quality string and eventual pair overlap informations. There are many existing tools that can do this job pretty well. I decided to start by using skewer a new tools recently published descibed to outperformed its concurents SKEWER. I may be interesting to try cutadapt after since it is a python script, but unfortunalty it isn't multithreaded and not developped for pair end data CUTADAPT. The problem with all these independant programs is that I will be forced to write intermediate files on disk (as opposed to stored in RAM). A possibility would be to use temp files Tempfile module.

2014 06 25

I tried skewer sickle and others but I'm quite deceived of their ergonomy, ie only adapters in terminal position can be trimmed and users needs to tell if the primers is present at 5' or 3' I finally decided to code it by myself in pure python. To do so, I designed a package named FastqQualityControl that contains 3 several classes:

QualityFilter : Takes care of filtering out a seqReccord if its mean quality is bellow a threshold.
AdapterTrimmer : Search for homologies between a fastq read and the possible adapters + find the longuest interval not overlapped by a match of adapters = Allow to find several adapters anywhere in the read
PairwiseAligner : Wrapper for Biopython Smith Waterman returning a list of matches// May be used for future faster implementations
FastqFilter : Integrate all other classes and extract reads by pair from fastq. If the pair can pass all filters it is added to a list of seqReccord. If no QualityFIlter object AND OR no AdapterTrimmer is given at object initialisation.

A comprehensive report and graphical outputs can be return at the end of fastq parsing.



In [2]:

    
from FastqQualityControl import FastqFilter, QualityFilter, AdapterTrimmer, PairwiseAligner

min_qual = 31
min_size = 100
input_qual = "fastq-sanger"
R1_path = "../test/fastq/med_R1.fastq.gz"
R2_path = "../test/fastq/med_R2.fastq.gz"
adapter_path = "../test/adapter.fa"
read_len = 150

a = PairwiseAligner() # VALID
b = AdapterTrimmer(a, adapter_path, min_size, read_len)  # VALID
c = QualityFilter(min_qual)# VALID
d = FastqFilter(R1_path, R2_path, c, b, input_qual)# VALID

e = d.filter()









    



	Extracting data
Uncompressing and extracting data
Parsing files and filtering sequences

Number of sequence analyzed :

1000
2000
3000
4000
5000
====== FASTQFILTER QUALITY CONTROL ======

  Execution time : 71.6677041054 s
  Total sequences processed : 5000
  R1 file : ../test/fastq/med_R1.fastq.gz
  R2 file : ../test/fastq/med_R2.fastq.gz
  Input quality score : fastq-sanger

====== QUALITY FILTER ======

  Quality Threshold : 31
  Fail quality filter : 3195
  Pass quality filter : 6805
  Mean quality : 30
  Minimal quality : 32
  Maximal quality : 31

====== ADAPTER TRIMMER ======

  List of adapters imported for trimming
    Name : Illumina1	Sequence : ATGCTGCTTA	Times trimmed : 831
    Name : Illumina2	Sequence : TTATCGCGTA	Times trimmed : 38
    Name : Illumina3	Sequence : TAGTGTTGTT	Times trimmed : 36
    Name : Illumina1_RC	Sequence : TAAGCAGCAT	Times trimmed : 828
    Name : Illumina2_RC	Sequence : TACGCGATAA	Times trimmed : 28
    Name : Illumina3_RC	Sequence : AACAACACTA	Times trimmed : 25

  Sequences untrimmed : 2967
  Sequences trimmed : 1679
  DNA base trimmed : 81563
  Fail len filtering: 807
  Pass len filtering : 872
  Total pass : 3839

====== PAIRWISE ALIGNER ======

  Parameters of pairwise2
  Maximal number of alignement : 5
  Match bonus : 2
  Mismatch Penality : -2
  Gap open Penality : -2
  Gap extend Penality : -2
  Score cutoff : 1.5



In [3]:

    
b.trace_graph()









    



	Creating a graphical output of read coverage after trimming



In [4]:

    
c.trace_graph()









    



	Creating a graphical output of read coverage after trimming

It work, but the SW alignment of long. Really long... more than 1 minute for 5000 reads. Basically it's not applicable for several million of reads... I tried with 500 000 sequences and It took hours...

Here is a example without adapter trimming



In [8]:

    
from FastqQualityControl import FastqFilter, QualityFilter, AdapterTrimmer, PairwiseAligner

min_qual = 31
input_qual = "fastq-sanger"
R1_path = "../test/fastq/med_R1.fastq.gz"
R2_path = "../test/fastq/med_R2.fastq.gz"

c = QualityFilter(min_qual)# VALID
d = FastqFilter(R1_path, R2_path, c, None, input_qual)# VALID

e = d.filter()









    



Uncompressing and extracting data
Parsing files and filtering sequences

Number of sequence analyzed :

1000
2000
3000
4000
5000
====== FASTQFILTER QUALITY CONTROL ======

  Execution time : 0.370543956757 s
  Total sequences processed : 5000
  R1 file : ../test/fastq/med_R1.fastq.gz
  R2 file : ../test/fastq/med_R2.fastq.gz
  Input quality score : fastq-sanger

====== QUALITY FILTER ======

  Quality Threshold : 31
  Fail quality filter : 3195
  Pass quality filter : 6805
  Mean quality : 30
  Minimal quality : 32
  Maximal quality : 31

I also tried with 500 000 sequences and it's less than 40 seconds... So it really comes from the pairwise alignment with biopython pairwise2.

I don't have time for now, but there are 2 choices to improve this part of the program:

Recode SW in python more efficient than the one from Byopython (Only one matrix alocated for all reads)
Code the algorithm in C and integrate it in python using Cython (Probably the best idea). Instead of the normal SW I think the alignment with less than k mismatch/gap may be sufficient and more rapid.

Since it is not critical, I will do that a the end of the developement if I have time.

Draft of ShortReadAligner Package

This package have to be able to accept a fastq list as seq reccord and to out put a SAM list as modified Seqreccord or by eventually using pySAM which i using Samtools. Since I'd like to limit the number of dependencies, I will first start by using only Biopython only.



In [27]:

    
from FastqQualityControl import FastqFilter, QualityFilter, AdapterTrimmer, PairwiseAligner

min_qual = 31
input_qual = "fastq-sanger"
R1_path = "../test/fastq/tiny_R1.fastq.gz"
R2_path = "../test/fastq/tiny_R2.fastq.gz"

c = QualityFilter(min_qual)# VALID
d = FastqFilter(R1_path, R2_path, c, None, input_qual)# VALID

R1, R2 = d.filter()









    



Uncompressing and extracting data
Parsing files and filtering sequences

Number of sequence analyzed :

====== FASTQFILTER QUALITY CONTROL ======

  Execution time : 0.000974893569946 s
  Total sequences processed : 4
  R1 file : ../test/fastq/tiny_R1.fastq.gz
  R2 file : ../test/fastq/tiny_R2.fastq.gz
  Input quality score : fastq-sanger

====== QUALITY FILTER ======

  Quality Threshold : 31
  Fail quality filter : 0
  Pass quality filter : 8
  Mean quality : 31
  Minimal quality : 32
  Maximal quality : 31



In [28]:

    
from Utilities import run_command

fastq_stream = ""
for i in range(len(R1)):
    fastq_stream += R1[i].format("fastq")
    fastq_stream += R2[i].format("fastq")

    
cmd = "bwa mem -p ../../../../bioinformatics/NGS/1_INDEX/hg19/BWA_hg19_canon/hg19.fa -"
a = run_command(cmd, fastq_stream)









    



An error occured while trying to execute the following command :
bwa mem -p ../../../../bioinformatics/NGS/1_INDEX/hg19/BWA_hg19_canon/hg19.fa -
[M::main_mem] read 8 sequences (1200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 0, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] skip orientation FR as there are not enough pairs
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::worker2@0] performed mate-SW for 0 reads
[main] Version: 0.7.5a-r405
[main] CMD: bwa mem -p ../../../../bioinformatics/NGS/1_INDEX/hg19/BWA_hg19_canon/hg19.fa -
[main] Real time: 1.986 sec; CPU: 1.972 sec

It seems quite complicated to use bwa on the stream directly from biopyton seqReccord objects... I finally think that it would be more universal to import filter and rewrite a fastq file with FastqQualityControl than to directly sent it to bwa standard input. Same thing for the standard output,

Possibility to use any other short read aligner
Possibility to run the module alone for the first time in order to obtain cleaned data to work with.

2013 06 26

Try FastqQualityControl with new version writting fastq files



In [3]:

    
from FastqQualityControl import FastqFilter, QualityFilter, AdapterTrimmer, PairwiseAligner

min_qual = 31
min_size = 100
input_qual = "fastq-sanger"
R1_path = "../test/fastq/1kseq_R1.fastq.gz"
R2_path = "../test/fastq/1kseq_R2.fastq.gz"
adapter_path = "../test/adapter.fa"
read_len = 150

a = PairwiseAligner()
b = AdapterTrimmer(a, adapter_path, min_size, read_len)
c = QualityFilter(min_qual)
d = FastqFilter(c, b, input_qual)
print (d.filter(R1_path, R2_path))









    



	Extracting data
Uncompressing and extracting data
Parsing files and filtering sequences

Number of sequence analyzed :

1000
====== FASTQFILTER QUALITY CONTROL ======

  Execution time : 9.87886500359 s
  R1 file : ../test/fastq/1kseq_R1.fastq.gz
  R2 file : ../test/fastq/1kseq_R2.fastq.gz
  Total sequences processed : 1000
  Input quality score : fastq-sanger

====== QUALITY FILTER ======

  Quality Threshold : 31
  Fail quality filter : 616
  Pass quality filter : 1384
  Mean quality : 30
  Minimal quality : 32
  Maximal quality : 31

====== ADAPTER TRIMMER ======

  List of adapters imported for trimming
    Name : Illumina1	Sequence : ATGCTGCTTA	Times trimmed : 57
    Name : Illumina2	Sequence : TTATCGCGTA	Times trimmed : 5
    Name : Illumina1_RC	Sequence : TAAGCAGCAT	Times trimmed : 62
    Name : Illumina2_RC	Sequence : TACGCGATAA	Times trimmed : 29

  Sequences untrimmed : 836
  Sequences trimmed : 112
  DNA base trimmed : 5632
  Fail len filtering: 51
  Pass len filtering : 61
  Total pass : 897

====== PAIRWISE ALIGNER ======

  Parameters of pairwise2
  Maximal number of alignement : 5
  Match bonus : 2
  Mismatch Penality : -2
  Gap open Penality : -2
  Gap extend Penality : -2
  Score cutoff : 1.5

Ok it works.

Try bwa command from an existing index and capturing the standard output.



In [5]:

    
import pysam
from Utilities import run_command
from multiprocessing import cpu_count

R1_path = "../test/fastq/100seq_R1.fastq.gz"
R2_path = "../test/fastq/100seq_R2.fastq.gz"
idx_path = "/home/adrien/Bioinformatics/indexes/mm10/Bwa_mm10_AAV_pAAV/mm10_AAV_pAAV.fa"
thread = cpu_count()+1

cmd = "bwa mem -t {} {} {} {}".format(thread, idx_path, R1_path, R2_path)

print (run_command(cmd, None, False))









    



@SQ	SN:AAV_RSV_GFP	LN:2216
@SQ	SN:AAV_RSV_GFP	LN:2216
@SQ	SN:backbone_aav	LN:2811
@SQ	SN:chr10	LN:130694993
@SQ	SN:chr11	LN:122082543
@SQ	SN:chr12	LN:120129022
@SQ	SN:chr13	LN:120421639
@SQ	SN:chr14	LN:124902244
@SQ	SN:chr15	LN:104043685
@SQ	SN:chr16	LN:98207768
@SQ	SN:chr17	LN:94987271
@SQ	SN:chr18	LN:90702639
@SQ	SN:chr19	LN:61431566
@SQ	SN:chr1	LN:195471971
@SQ	SN:chr1_GL456210_random	LN:169725
@SQ	SN:chr1_GL456211_random	LN:241735
@SQ	SN:chr1_GL456212_random	LN:153618
@SQ	SN:chr1_GL456213_random	LN:39340
@SQ	SN:chr1_GL456221_random	LN:206961
@SQ	SN:chr2	LN:182113224
@SQ	SN:chr3	LN:160039680
@SQ	SN:chr4	LN:156508116
@SQ	SN:chr4_GL456216_random	LN:66673
@SQ	SN:chr4_GL456350_random	LN:227966
@SQ	SN:chr4_JH584292_random	LN:14945
@SQ	SN:chr4_JH584293_random	LN:207968
@SQ	SN:chr4_JH584294_random	LN:191905
@SQ	SN:chr4_JH584295_random	LN:1976
@SQ	SN:chr5	LN:151834684
@SQ	SN:chr5_GL456354_random	LN:195993
@SQ	SN:chr5_JH584296_random	LN:199368
@SQ	SN:chr5_JH584297_random	LN:205776
@SQ	SN:chr5_JH584298_random	LN:184189
@SQ	SN:chr5_JH584299_random	LN:953012
@SQ	SN:chr6	LN:149736546
@SQ	SN:chr7	LN:145441459
@SQ	SN:chr7_GL456219_random	LN:175968
@SQ	SN:chr8	LN:129401213
@SQ	SN:chr9	LN:124595110
@SQ	SN:chrM	LN:16299
@SQ	SN:chrUn_GL456239	LN:40056
@SQ	SN:chrUn_GL456359	LN:22974
@SQ	SN:chrUn_GL456360	LN:31704
@SQ	SN:chrUn_GL456366	LN:47073
@SQ	SN:chrUn_GL456367	LN:42057
@SQ	SN:chrUn_GL456368	LN:20208
@SQ	SN:chrUn_GL456370	LN:26764
@SQ	SN:chrUn_GL456372	LN:28664
@SQ	SN:chrUn_GL456378	LN:31602
@SQ	SN:chrUn_GL456379	LN:72385
@SQ	SN:chrUn_GL456381	LN:25871
@SQ	SN:chrUn_GL456382	LN:23158
@SQ	SN:chrUn_GL456383	LN:38659
@SQ	SN:chrUn_GL456385	LN:35240
@SQ	SN:chrUn_GL456387	LN:24685
@SQ	SN:chrUn_GL456389	LN:28772
@SQ	SN:chrUn_GL456390	LN:24668
@SQ	SN:chrUn_GL456392	LN:23629
@SQ	SN:chrUn_GL456393	LN:55711
@SQ	SN:chrUn_GL456394	LN:24323
@SQ	SN:chrUn_GL456396	LN:21240
@SQ	SN:chrUn_JH584304	LN:114452
@SQ	SN:chrX	LN:171031299
@SQ	SN:chrX_GL456233_random	LN:336933
@SQ	SN:chrY	LN:91744698
@SQ	SN:chrY_JH584300_random	LN:182347
@SQ	SN:chrY_JH584301_random	LN:259875
@SQ	SN:chrY_JH584302_random	LN:155838
@SQ	SN:chrY_JH584303_random	LN:158099
virus|00|0|1-150=AAV_RSV_GFP:1922-2072	83	AAV_RSV_GFP	1923	0	150M	=	1406	-667	AGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATTTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTCAAATGTGGTAAAATCGATTAGGATCTTCCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTT	:7?>@<0)4F<5DEF;>67=6B::<;@C<ICIAII7;@?DF>?<GD<III94833@ABIIB=?>:0;<;?==>=@?<A@>CE>?BFCCB@BCECCCDCDDAAEFEFCDCBEFFGFFHCB@@DDDCDEFEEEGIFHCG?AABED@@@>?;<	NM:i:1	AS:i:145	XS:i:145
virus|00|0|1-150=AAV_RSV_GFP:1405-1555	163	AAV_RSV_GFP	1406	0	150M	=	1923	667	TCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGCGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAATGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGC	EB=C<>=@A?BDGBEEEACEDDEDDCDDEACGFFEEIIEFEEFFDEEGHFEFFCCCCECC@C?A<>BF@EE@?B?@CBEIEIBCAA@?@@EA@A99C:;BE9CDIIGCHICC>927D934138?ICAFII<=7><5052695;68B/440	NM:i:2	AS:i:140	XS:i:140
virus|01|0|1-150=AAV_RSV_GFP:28-178	99	AAV_RSV_GFP	29	0	150M	=	210	331	CCCGGGCAAAGCCCGGGCCTCGGGCGACCTTTGGTCGCCCGGACTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCCGCATGCTACTTATCTACGTAGCCATGCTC	B?@@BG@A=AD@@DC@BDD>CBBCBDDDDECBDDACDFEEFFECDEEFEGFEBAGCCGIHD@C@A=@A@<79<AAA=BE?9=8@D=;7<19@@GEI?A??51.:34<;97/<;89;;5BDC<6F@<E@4/@><2)3,39:5BHDCAEI5<	NM:i:2	AS:i:140	XS:i:140
virus|01|0|1-150=AAV_RSV_GFP:209-359	147	AAV_RSV_GFP	210	0	150M	=	29	-331	CTTATCATCGCAGATCCGTATGGTGCACTCTCTGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCG	37;5?=9>8A<::B6>>6=:?=;<1=:;8:1/8=C>72;2768>EGE;DEAEC84=:47:9=@G<?>CAA;;>CB>@@CFHEFHDEDDEG@?CFFIGIEBCDGEFEEFFC@@DDFHGIGBABEEEIGCACDDEEDCC?ABCAAAAA<B?C	NM:i:1	AS:i:145	XS:i:145
virus|02|0|1-150=AAV_RSV_GFP:1012-1162	99	AAV_RSV_GFP	1013	0	150M	=	1223	360	GGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCA	?<BA?BC@=<ABD?ABBEGHEFECDDBDDDDBAEEEDDBDEECHDFED@BDCCEEBCEEDDFGEEA?>GICADGIIE?A?BEIIIB@<89=38<?F9;@<?IIIDHHI@C<BII=@>ACEA9748>;?9<66?CA79DC?526/4<D?AG	NM:i:1	AS:i:145	XS:i:145
virus|02|0|1-150=AAV_RSV_GFP:1222-1372	147	AAV_RSV_GFP	1223	0	150M	=	1013	-360	GCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCG	@;:<>50-A3-205119>?>D<>?@>=>=>9:8>AIFB<B>33BC>C9-0EDCG=@@99;>E@3:<AACEIDAB<>;:I@DCDD@ADEDD@BCEGEEEICBA?CFDCBCDBABCADFHFGHFEEBDC@>?DEEF?BA>@GI>A=<A@BB?	NM:i:0	AS:i:150	XS:i:150
virus|03|0|1-150=AAV_RSV_GFP:1558-1708	83	AAV_RSV_GFP	1559	0	150M	=	1329	-380	CCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA	I20,*-400:@EE6==:4EC@?B/2*.7:19;ABA?@:<:;;7>A7??IG=>@FE>::@;BFCFD?@ADFIGGAABAA?CA?AAABACCCCCHEFEFHDGCDDFGDEFEDFEGEEBCAEFHHCDDCBBDGCGEFGCHHBFDG@@<>=<55	NM:i:0	AS:i:150	XS:i:150
virus|03|0|1-150=AAV_RSV_GFP:1328-1478	163	AAV_RSV_GFP	1329	0	150M	=	1559	380	CGACGGCAACTACAAGACCCGCGCCGAGGTGCAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATAT	>=<>ACCCGFFABGGFDEEHFFEGGGGFEDDEDCHCBHGCHFEECDACADGEGEBCCCE@@;:?AC>DC@AAA?:8@AD>@C?FEBFC?C?A<BA:A@ICA8EIIEDE:=A?>DH@>><?C@HBB=9-*?G<:<067A;>C091*)(#,&	NM:i:1	AS:i:145	XS:i:145
virus|04|0|1-150=AAV_RSV_GFP:1174-1324	83	AAV_RSV_GFP	1175	0	150M	=	1001	-324	GCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCA	3;B3450*10D:>;6/&;1;A<HC9>:@=G?A==?C<EIA>?AHIDB5;?EFEIIIIFBI@A:F?@GD<9IIH<9BEE@<?<CDHCCDEGFCCFGCEEABEFHGFFDEDDEDEFGGFECEDDDCDEDEEFGEECDHEFE@?<>@?B><?@	NM:i:0	AS:i:150	XS:i:150
virus|04|0|1-150=AAV_RSV_GFP:1000-1150	163	AAV_RSV_GFP	1001	0	150M	=	1175	324	AGCTGCTGCAGCGGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGCCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGA	>@??CD?A?>?@?ABCFDCCDFBAIGDBCDCDCDCEGDDCBCDCCDGIDEIIGGFCCCCHGEDEDGC=;9;AAFGHEDHAH>AA:><IDEIE?><AFEID><0-6,4A<;544?=B:48?FA?76;8@>04;.833B7=<:;54035?E5	NM:i:1	AS:i:145	XS:i:145
virus|05|0|1-150=AAV_RSV_GFP:605-755	99	AAV_RSV_GFP	606	0	150M	=	696	240	CCAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCT	??=><@BAB@@A=@@=ACACFEGBCABFIHGFFFFEDBDDDDCADDFEGGDDBBBAA=>?A@@@C@>=??>AFIEED?ECB@?=61HD>>G?@D;FII@AAC?D;840<:<?<?=><6016DIC-<<8:;A-6;7429;@=?<:=954:6	NM:i:1	AS:i:149	XS:i:149
virus|05|0|1-150=AAV_RSV_GFP:695-845	147	AAV_RSV_GFP	696	0	150M	=	606	-240	ATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGATCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCA	508988;:B2DGF>-,,=9;97A266A7?/<?<>255.<:7;8B@@@AD<??916=>=IBI??D:CFCGIACEDCBGGACCCDDDAACCBABEBCDCDCDCBEFCA?FEDCAEHFGGEEFGEDDDECBAEECB@DCB?@CB?A?ABABDB	NM:i:1	AS:i:145	XS:i:145
virus|06|0|1-150=AAV_RSV_GFP:1897-2047	99	AAV_RSV_GFP	1898	0	150M	=	2067	319	TAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTCAAATGTGGTAAAATCGATTAGGATCTTCCTAGAGCATGGCC	?A?@BCBBCD@AAA?@ABEEEGBCDD@CADDCEBCDGFEGFEEEGFEEEDCDGFEEDCEIHA?BBBDD?AABBFIH?>BBCBC@<C>=;><:DI>?:47GE??ID8=2?=>;8?A93;38.>I9;I9=;?7338123B720+372?<@,8	NM:i:1	AS:i:149	XS:i:149
virus|06|0|1-150=AAV_RSV_GFP:2066-2216	147	AAV_RSV_GFP	2067	0	150M	=	1898	-319	CGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAG	9IH??9:?7@?:==;4:>9>@F?C=>;DA@A@A82/+.2747?:=;77<<FIIIEIIF>?BEE9?FHDIE9EFD@<AA;=A@=@AEBAD@DCBECCADDGFFEEEDCBEFECBAAGCEDHGHHIHHCBC@BCBCDDDABBA@C@=@>>B;	NM:i:0	AS:i:150	XS:i:150
virus|07|0|1-150=AAV_RSV_GFP:767-917	99	AAV_RSV_GFP	768	0	150M	=	1019	401	CGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTG	=>>@?>CBDDE?DAADDCBFEEDGFEEAD?EIHFGFCDDDFFCCGFEHGIIFECGBDCBIDCCFD=@A?FFD@=><BFAGD<?<@>?:>?D?=?CIH>IIFA8<@<AFA<DB<<@?7A=H@@GIHI>>HB5.+6,256.+3'6/378E<<	NM:i:0	AS:i:150	XS:i:150
virus|07|0|1-150=AAV_RSV_GFP:1018-1168	147	AAV_RSV_GFP	1019	0	150M	=	768	-401	TCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTACACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCCGACCCTGAAGTTCATCTGCA	A3*1AH@7B5/6394;;;>7?<91?;<B9:E=;;.-5:49F8?70828>=<AEA9?:<@ID?FBC;>;CIHGCBCICB:ABB@CCDG@<CBCECCEEF@@CDFCBEECGCCBFGIECDDDEFFGFGGFFDEDDEB?A?BDDEBAA@A@<9	NM:i:2	AS:i:140	XS:i:140
virus|08|0|1-150=AAV_RSV_GFP:754-904	83	AAV_RSV_GFP	755	0	150M	=	615	-290	TCGATACAATAAACGCCATTTGACCATTCACCTCATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATA	CD98A51(>;7;;65;6=69;:;<<IA>GII?><<ECHF68;52:087>?<;=5;5B9DFB77=<:=BI<9:;=>@?A9:39GEFEC@DAFBAFDDCAB@DFFGEEEFGCB@BEAEEEDGIGEEDCBDFCAECBEEBA<<??=@AA;;=8	NM:i:1	AS:i:145	XS:i:145
virus|08|0|1-150=AAV_RSV_GFP:614-764	163	AAV_RSV_GFP	615	0	150M	=	755	290	GAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGATGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAAT	AC?>A=CDCB=CB?AABCEFDDDHHGIFGFFDFFFEGHGFEFFDECCDEBAACFDDGHFEDGDDCDDB>>CD=CBEIG<IIDIIFC@<FGFD@@AID=>GC==6:<>8?7B?>8022A<@C@52792'49;?51=98<?7A;=6=862;5	NM:i:1	AS:i:145	XS:i:145
virus|09|0|1-150=AAV_RSV_GFP:676-826	99	AAV_RSV_GFP	677	0	150M	=	778	251	GGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGC	@?C?DEHC@>@@@BACFDGBCDDDDDCDBCCGIEEEBBABCAEEEEEDFFFEGHCCDG@ABCCCCBDCB?FIBBA<B>AB@EFA9==HEBFIFFGDIID:3FCIDIG>7>7=?BC@A8>?C:;:C<:;486@7B;ED<8?:;H>;=7865	NM:i:0	AS:i:150	XS:i:150
virus|09|0|1-150=AAV_RSV_GFP:777-927	147	AAV_RSV_GFP	778	0	150M	=	677	-251	CCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGA	9D:8:448A<HA8FHFA1=A65:9DC@=G<;E??DB0==533507<256@B?<=@;IDC8@@@?G<<<>A:?CBD@<=AHFFECCBFGBAC>DFDDDDDDFFDDDEEFCFFDGGDDDAFDDCBBEAEEBDEEEEFGHFB@:A?=:=>=<8	NM:i:0	AS:i:150	XS:i:150
virus|10|0|1-150=AAV_RSV_GFP:1840-1990	99	AAV_RSV_GFP	1841	0	150M	=	1998	307	GAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTACATTCATTTTATGTTTCAGGTTCAGGGGGAGACGTGGGAGGTTTTTTAAA	C:;=@@@>?<?ABCABFEFBFEDDEFEDCB>BCFGCDIFCBACBCGFBCEDDDBFEC@D@CC?BGEDBIF>=CDCBCHC@9:=;@<?8;>E>:BBIEI>>DB??<<167D;@>=7>ACIE8H8>609296=<CII?5C?<9&55G=891>	NM:i:2	AS:i:140	XS:i:140
virus|10|0|1-150=AAV_RSV_GFP:1997-2147	147	AAV_RSV_GFP	1998	0	150M	=	1841	-307	AAACCTCTCAAATGTGGTAAAATCGATTAGGATCTTCCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGC	;G?4;DE765=<;::0656697CGDDCIIAACAA::7G?@;=FIGGGDD=E>?>CI@:9:;FIIDEA?=A>=?C?<<7>FDBC=B==EFDBA@BGDECDCFHEECFEDCDGEFDDHFEEABBCBCBAHIHDFGGIGEDGFCD?=>AC@B=	NM:i:0	AS:i:150	XS:i:150
virus|11|0|1-150=AAV_RSV_GFP:1746-1896	83	AAV_RSV_GFP	1747	0	150M	=	1386	-511	GCCGCAGTTACGCTATGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT	.=*(,1.-803<GB=3>G?78?4+);9B:>93=DFBCB>AE@I:@CDF?>BDH:IGG87CBDICB<>;B;C@EHCEGDBBAA=>:@?AA@B@@CDECDFEFHHHGEFIEGIDDC@DDFGECBEDAABEFGEGCEEFA?BABCD?>??=<B	NM:i:1	AS:i:145	XS:i:145
virus|11|0|1-150=AAV_RSV_GFP:1385-1535	163	AAV_RSV_GFP	1386	0	150M	=	1747	511	CCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTGAAGATCCGCCACAACAT	CEA@<:==C@;?>>ACCDBCDEHFEEDDCDEFEEB@BEFGED@CDEDEHIGFBCDDECCDBFDDHFHBADABC??@A:?8@?F>B?;9:D=?B?8EG97<5@<=ADA>9>BFHE7;G>0@<F3>G9.:@;36B215A</:?<?IA@<@II	NM:i:1	AS:i:145	XS:i:145
virus|12|0|1-150=AAV_RSV_GFP:1012-1162	99	AAV_RSV_GFP	1013	0	150M	=	1237	374	GGGAATTCATAGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCA	?@@@D?@@?B==BCCD@ADBCDDDEIHFHGIGFACEHECCDDEGFCBCACDCDBDDFHFFE@CFDFDEDI<<;A=?BC<==DIFEEABBC;<68<BED=H>AI;=<A@??A?C9<?78GB6:=:/1:<>;1/4=96=<@??042)-5053	NM:i:1	AS:i:145	XS:i:145
virus|12|0|1-150=AAV_RSV_GFP:1236-1386	147	AAV_RSV_GFP	1237	0	150M	=	1013	-374	AGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAAC	306)22159ADB<C<;<C>32-1=AGI>84@74<<:7?89+4@C7>>=9A?AEDEEA@<C;@@FIBA@@@D==E>CC@>BEGBFHIGDEB>?BAEFDDDCEFEFC@@@@@DDBABCCDGGDDEEFEGGDDDCFEEBBACB?>=?>;89B?	NM:i:0	AS:i:150	XS:i:150
virus|13|0|1-150=AAV_RSV_GFP:1168-1318	99	AAV_RSV_GFP	1169	0	150M	=	1322	303	CCACCGGCAAGCTGCCCGTGGCCTGGCCCACCCTCGTAACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGGCTTCTTCAAGTCCACCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCT	<=>AGFCABACDDIEEFABCFBACEEEAABEDBCDGDEEHFDDDCDACDCEFBDEBCBBBDEBCEHC>8=?GBHEEC?<@DADA@II@@A<E:9<@BB@?A@=:>@CAA?59717:3383;F>:=/9,8A5C4;=:@@9DIBDB>9779>	NM:i:4	AS:i:130	XS:i:130
virus|13|0|1-150=AAV_RSV_GFP:1321-1471	147	AAV_RSV_GFP	1322	0	150M	=	1169	-303	TCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCCACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCGCAACG	0C;;;:/+5=CIDIF>;<@BA2)+)48>AF<?;DEGHD:7B324-<3>>=DAIA=:9@;6:968;031<;<BCBB?HE?BDDEBDEEFBEGDGEEICGDDDCBECACHCA>DDCBADDCBCDFGCBCDADDEFDFHCBDDDA@A=;AB>C	NM:i:2	AS:i:140	XS:i:140
virus|14|0|1-150=AAV_RSV_GFP:772-922	99	AAV_RSV_GFP	773	0	150M	=	894	271	TTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAG	AB;<<??=@C<>?@BBACEEHBCFEFFCCDCF@BCDDCCHFEEEFEDFBCFECCFECHIFGGFE@AACED@7=?@?BDCDGBIIHCCIF=CB@D:C@950AD@GB?HGGB>4>>E<>I<9=74/6;9E97GG83817894/73<7-30:6	NM:i:0	AS:i:150	XS:i:150
virus|14|0|1-150=AAV_RSV_GFP:893-1043	147	AAV_RSV_GFP	894	0	150M	=	773	-271	GGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGTACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGCAGCTGCTGCAGCGGGAATTCATGGTGAGCAAGGGCGAGGAGCT	3<4<=@?7859AD@B::AG86@9@=A:4@4=;>EGBA:?@E<BD>1248????BA@BBD<9>=>BCEIDGICDBE@?<AAEDEIBBAEFCAFHEGEFCBDDDADEFDDEFHIEGEEFFDDDC?CEFFGDFGDCBAB@A?AEEC@?<;=>@	NM:i:1	AS:i:145	XS:i:145
virus|15|0|1-150=AAV_RSV_GFP:715-865	83	AAV_RSV_GFP	716	0	150M	=	358	-508	AATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGT	>:7627:;BI?:7;??E<94<FDI=?C:<A:==<@=<69B;@@E:>;I:>C=;=>?EEAF@IIBBDFHDE@??=@A@HHH?GGCCDFEEDDGIHGIIDDDDGEDAADDCADGIGCCEFCDFDDCDDDDEDDEEFCBEFIEACACD@@?=<	NM:i:0	AS:i:150	XS:i:150
virus|15|0|1-150=AAV_RSV_GFP:357-507	163	AAV_RSV_GFP	358	0	150M	=	716	508	CGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTCCGGGCCAGATATTCGCGTATCTGAGGGGACTAGGGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGAT	98@A;>@=?<A?EIDGFGEFCDDBHHAFDBFFEEBBABFGFEED@CCBCEEFEEDDDFIHFEFFHCC@;7;<A=CIFHBCBI>?3:>DFADDBI<<577B84DDE;>F8;<8C;7>B?<@4<;@9<??5735;B;;80>3;4?G??>B2/	NM:i:1	AS:i:145	XS:i:145
virus|16|0|1-150=AAV_RSV_GFP:2045-2195	83	AAV_RSV_GFP	2046	0	150M	=	1848	-348	CTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAAGAACCCCTAGTGATGGAGTTGGCGACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTC	@=;8D@99DIIC<;>A=:9:BECIFHG=1(/8@:;4B==IHFEC;BAC>GIBCBE=2>IEA6:A@@@>>@I>=8=;>AEBCCFDGGIH?CBHDDEDFIGEEEDDEEEFEGGDCDDDDDCECEDCGIIEEBCECACBA?<?@AA=A?=?@:	NM:i:2	AS:i:140	XS:i:140
virus|16|0|1-150=AAV_RSV_GFP:1847-1997	163	AAV_RSV_GFP	1848	0	150M	=	2046	348	GTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATATACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGCAAGTA	???7>@CC=FAFCC@D@DCDCGEGGIGGFEEFEEFCCDDCABBBCEEGFDFFCBEABDDGBBCCBBB?;FCD?8@B>?HHE=DDIIDB:@HACABA<9=;<CIIHGF99@AHI58E9IIBI>DIC246EE@;>?F407@DH>@56?3)*4	NM:i:1	AS:i:145	XS:i:145
virus|17|0|1-150=AAV_RSV_GFP:755-905	83	AAV_RSV_GFP	756	0	150M	=	482	-424	CGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAG	;6E?87632:G<5??9=2.>@@;5@FF>?D>C<276;3A6<30;:><;:GEIE?AFB;6=I?@=><DCA:=<<=74:=CCDD?@ACFECBGGCCDEFDCGDHEHEDEDHGCBDFABCCCABCFEECBDACBCCD@>A@BB@DCF@;==?:	NM:i:0	AS:i:150	XS:i:150
virus|17|0|1-150=AAV_RSV_GFP:481-631	163	AAV_RSV_GFP	482	0	150M	=	756	424	TACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACGTGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACGGTGCAT	?>?@@=A?DDACCB<=ABBEGFEEDCDEFFDCEEDDDCBFGFEEGFDDCDEFDDDEE@AD=EFCB@A=>CG?D=?>?BCG>7;>H@B?I??CA;BCGCDA:;5=:GBAI<7D3@:/6<8:<:=8>G;<AH=@DA9<48/;?<<1=.8?=>	NM:i:2	AS:i:140	XS:i:140
virus|18|0|1-150=AAV_RSV_GFP:1491-1641	83	AAV_RSV_GFP	1492	0	150M	=	1253	-389	CAGAAGAACGGCATCAAGGTGAACTTCCAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGTCCACTACCAGCAGAACACCCCGATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCC	3-0623=/697;14CH9A:2=;<<=?87509AA?:CAD<>>)40694+:5DIB>FCFDA<D@=?E?ADEF@@BBFCHIACADCDB@GEGDECDBDEEEEFDEEFFGEFGHFHHGFEFECEABDFFEIDDBDDEGDC@A?AEB=::><>:7	NM:i:3	AS:i:135	XS:i:135
virus|18|0|1-150=AAV_RSV_GFP:1252-1402	163	AAV_RSV_GFP	1253	0	150M	=	1492	389	ACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGG	==@AA=@@ACBCCBCCGFEEEEFECBCDDCCDBECCCGFGHHECDDDDFGFEEEEAAABBDEDIFGEEB<:@DDICBCBD>;B;F;>>B?<@I@?4:<@?EB72/3265<FC@?@FCB@I9HB;A?8,52030/1+/.A::<:9>6%/(2	NM:i:0	AS:i:150	XS:i:150
virus|19|0|1-150=AAV_RSV_GFP:1723-1873	99	AAV_RSV_GFP	1724	0	150M	=	1806	232	ACGAGCTGTACAAGTAAGATGCGGCCGCAGTTACGCTAGGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAA	?:>??BAA?DDFDDEDAC?CD?@@EEEBCFGFGFBBDBCFEEGFECDDDDDDHEEDDBBFCHCFBCAD@?AC=::EEAD:9;AFIEB?;>HI>:BHGGFGIDAIDADAEFCAGAI4?=D=0B10404128>39=<:;C>6*6<3HIA965	NM:i:0	AS:i:150	XS:i:150
virus|19|0|1-150=AAV_RSV_GFP:1805-1955	147	AAV_RSV_GFP	1806	0	150M	=	1724	-232	AAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTT	;D=88/4<@>5<?9<8/4<><==?BC9<<I<7<:@HD==I>B?AA>BG?;88;3;ECBE?:<7HIGGI=6;@A@FCEA><@;>@CAD?A?A:B@BBDFFEFDB@EDADDDDDEDEDDCDCEIGEDCDDECFBDFE@CEA>AA=@<BE?CH	NM:i:0	AS:i:150	XS:i:150
virus|20|0|1-150=AAV_RSV_GFP:1769-1919	83	AAV_RSV_GFP	1770	0	150M	=	1676	-244	AGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGTCAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAA	87=?:D>;4?CC;7-<97?B6%&$4:<;>9;<;FF@C>?@AB<278;=@BCHBAAIFIIBIE>C98A<=>GFE?;BAEDBBIEEBCCAAEFFFDDBEFEFADCDGDCBDCDCBDDDFGDECCEFGIFFDEFCBEDCDCB>@>BAAD><<=	NM:i:1	AS:i:145	XS:i:145
virus|20|0|1-150=AAV_RSV_GFP:1675-1825	163	AAV_RSV_GFP	1676	0	150M	=	1770	244	TGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAGATGCGGCCGCAGTTACGCTAGGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTG	?@CA:B=@DD=@DCGECGFECEGHEDDFFEEGCFEEEGHFEIHHIFECDBBDDCBDABBEGFAHEFDI@<AI@CC;?7B?AB<?=;BC:AGB:=F>A;F?I?GCCDAA<=7=FIAAIDA=:8;>II<AEC?;6>>A@7317,:43=857=	NM:i:0	AS:i:150	XS:i:150
virus|21|0|1-150=AAV_RSV_GFP:938-1088	99	AAV_RSV_GFP	939	0	150M	=	1170	381	CGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGCAGCTGCTGCAGCGGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGT	@?;:<><BCBAEDD??DDEIGEEEDFHCBCEEGFECBBB@@BDECDABBCDDEEEDGHFAA@=<?ADDCA:B@FBABC@?A><DCICBC@DFB?BGACA?=:B==AC752/75A<8E@E@?9<@;4-56>G@>EIIA@:8><BAB972*9	NM:i:0	AS:i:150	XS:i:150
virus|21|0|1-150=AAV_RSV_GFP:1169-1319	147	AAV_RSV_GFP	1170	0	150M	=	939	-381	CACCGGCAAGCGGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGTAGGCTACGTCCAGGAGCGCACCATCTT	686F<25*.0@6;;<6=C=?C9;9BD63/1DD=2><;C>9AIB==A@?ID89;A@F?ID?@=AGEHH=ACABEID??>??IACBE@BHGEBDDEEADDEECB@ACE?BDDFCFGCDDDDDDHGCDDEGDEDCBDCA?AF?AAAA=;9?=>	NM:i:2	AS:i:140	XS:i:140
virus|22|0|1-150=AAV_RSV_GFP:450-600	99	AAV_RSV_GFP	451	0	150M	=	692	391	TGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACAT	?B>@A==?EDIEIHIIGIB@BCCDBEDBCCBADFFHGDCEEEEEEEFEEABCEEEDCBCFIDEEAA@?BA>@=?AAA79BIE=9=?>?A6;?@BAF?@=?>=>0>?AA<BC8DAC@HEED@5>98B5061;==B9IA><18CH>@?2861	NM:i:0	AS:i:150	XS:i:150
virus|22|0|1-150=AAV_RSV_GFP:691-841	147	AAV_RSV_GFP	692	0	150M	=	451	-391	TGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGC	<:8B55.59953AB2+,:0:44;5%:;;=>@<:8F:75I?D:?FII=BII=<02=@ABE89628?B=:BAGGFAACC=<BBGFBBBDDCACBBCCEFFGGGHIGEDHECBCDFCDDCBDDDEFECBCDCDDB???B@CCBB?CEE>=;?@	NM:i:0	AS:i:150	XS:i:150
virus|23|0|1-150=AAV_RSV_GFP:1455-1605	83	AAV_RSV_GFP	1456	0	150M	=	1232	-374	TACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTG	6D@A::?C4?2@E@;<D<:@3>840-@79<6B?BB8:=>@@E@EB?8@AH:@>DFE>==?DBBCHGAFBG<8:AGDA@=EACDFDF@BCDAEECCDCGEEC@DCEDEB@@CBDFDDCCDHGGEGFCDCDCBBCA=DECDDAD??7;A@@>	NM:i:0	AS:i:150	XS:i:150
virus|23|0|1-150=AAV_RSV_GFP:1231-1381	163	AAV_RSV_GFP	1232	0	150M	=	1456	374	GCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGAGCCGAGGTGAAGTTCGAGGGCGACACCCTGG	@=<?@@C==>CDDBC@BBBCCDECABCEEEDBBC?BEDFEFBCDECEAFHDFHFFEEGIDBDDHI?BACC@>>BDDCDD>BBFII>?:?><:I=;C?C>>=39@A@;?C==99><?<D=606G;B+58C43D44=@?::?<02:A<7E@C	NM:i:1	AS:i:145	XS:i:145
virus|24|0|1-150=AAV_RSV_GFP:538-688	99	AAV_RSV_GFP	539	0	150M	=	712	323	AAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGG	AA?A>@<=?@DDFEGGGFBCCDDECBCAFEEFDDEFECDCEDEDFECFFFDEEEGEBCCCCDCFDEDCCAAFDBAEBC@6=7A3>AEII=??A@@BDDH>DADCIE49;DDEBAFC>FHG=1606>0:=6<<7:@=@=C?71879*2;E?	NM:i:0	AS:i:150	XS:i:150
virus|24|0|1-150=AAV_RSV_GFP:711-861	147	AAV_RSV_GFP	712	0	150M	=	539	-323	ACTAAATTCCGCATTGCAGAGATACTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCACCTGGAGGCACTGGGC	@:;ECC6459F;G:3:4:H7HE;GFBD=8A;8AF?983<?F@??@@B=;<@B9CICCDG;<>;@G>CFFGA?9?BBCBA><>BEHDEDDDDEFDDBEFEDDDFFEBCEEEEECBDEEEFCFFEFDCEEDFEEDB?@@BA=@>?>@?>=@F	NM:i:2	AS:i:140	XS:i:140
virus|25|0|1-150=AAV_RSV_GFP:2066-2216	83	AAV_RSV_GFP	2067	0	150M	=	1898	-319	CGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGAGAGCGAGCGAGCGCGCAG	CC>==<7<9'6-434B74/:7BB?IIG8;EGCI?;46B5<EE>=;C73@>>/?<7:DBD=89C?F@C=B:=<8?A@A@>DADECDEAEFIFCCBBGCCEEFDCFGDDHFFBCCBFDDDDHEEEFGFEFHHHIGHCCB?@@CA>BC::>=?	NM:i:1	AS:i:145	XS:i:145
virus|25|0|1-150=AAV_RSV_GFP:1897-2047	163	AAV_RSV_GFP	1898	0	150M	=	2067	319	TAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTCAAATGTGGTAAAATCGATTAGGATCTTCCTAGAGCATGGCT	@=A@?B@7;>?AEDBDEDEGEEDD@CDCCDBGHIEEDDDCCDEEDEBCC?@DBEGBC@@@EDFHGECDIIGCAA=DDIIEAIDIHHECC:<=CII8;:>;?AA>>?7?AD;8?IF;<48B5?E>3-4C=?@>:4D89CE868.023&'1*	NM:i:0	AS:i:150	XS:i:150
virus|26|0|1-150=AAV_RSV_GFP:370-520	83	AAV_RSV_GFP	371	0	150M	=	194	-327	AAGAATCTGCTTAGGGTTAGGCGTTTTGCGGTGCTTCGCGATGTACGGGCCAGATATTCGCGTATCTGAGGGGACTAGGGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGC	=335=AA=6<H?EGIE?:AIBI8=EBB=I@<;BG=B:6ABD9A@A946?@<IE9B@EH?B603885;;EIEIIEHAEBB;;DFIBFADD?CFHEFIIFFHEEFFFFGECDCBBDDBBBEEDCCEECCABBDDGGGB@EEEFBCA??@<DA	NM:i:1	AS:i:145	XS:i:145
virus|26|0|1-150=AAV_RSV_GFP:193-343	163	AAV_RSV_GFP	194	0	150M	=	371	327	GCGTCATGTTTGACAGCTTATCATCGCAGATCCGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGATGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACA	7:?A?=?@A@ACCC=BAACDD?CDDDEEGHDADDECHFFABBCEGIGFDAECCHGED=BCFC>BCIDCAAB=@?DAD@E4>?@HIHI=@@DBI81;<AC?=FCCAI;;:4=B?><=>EA@@4:HB?G=GIBA:9=>6AI>=IIA;@A=B?	NM:i:1	AS:i:145	XS:i:145
virus|27|0|1-150=AAV_RSV_GFP:121-271	99	AAV_RSV_GFP	122	0	150M	=	449	477	GGGGCTCCTTGTAGTTAATGATTAACCCGCATGCTACTTATCTACGTAGCCATGCTCTAAGAAGATCTCGACGCGTCATGTTTGACAGCTTATCATCGCAGATCCGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTA	A@BCB>@??D><AEEIHFCGHFDFFGCEECDDCFCEDBBBBCDFEDEFCECDECCCCEFCEDDIHEB?;BBBDC>=>>@5?0?C><:@>4ABCHBI279@;AI??BB5;=?F:H@@DE;535DA692?;;<;B8AE;0;7/162:1:89A	NM:i:2	AS:i:140	XS:i:140
virus|27|0|1-150=AAV_RSV_GFP:448-598	147	AAV_RSV_GFP	449	0	150M	=	122	-477	GGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAAC	.1=@3<=5:>673:D;E=9BIG@EE0>8:71904?<@B7=6@802CBAIECHE=?@B<;DIBCIE>>7<@?CE;>CDG>DDFDEHECGBDEGDABCEEDADIFFFEEEEFEFEECDCCFDDFGEDDCDDDFEEGFB@<;A?<@@EEB@BD	NM:i:0	AS:i:150	XS:i:150
virus|28|0|1-150=AAV_RSV_GFP:1183-1333	99	AAV_RSV_GFP	1184	0	150M	=	1308	274	CCGTGCCCTGGCCCACCCTCGAGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACG	:<DACBD>B>@ABCCBBCDEDFDDCIFDFDCDEFDBABEDDBEEFFFEEHGIEFGDEEFHGIFGAA<=C@=EFDFFDDCGGDD:=F:?;=6GBIDIDFIB-2;>FCBGICF>::8:;69=:<IC3:2337=IG@<BE<:490=?D8>0,.	NM:i:1	AS:i:145	XS:i:145
virus|28|0|1-150=AAV_RSV_GFP:1307-1457	147	AAV_RSV_GFP	1308	0	150M	=	1184	-274	GCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGGGGGCGCCACCCTGGCGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGCCAACATCCTGGGGCACAAGCTGGAGTACAACTA	:B5906@F<4'.1/5?63365*8=;2)=/3;?:IAA;:<:EFIDEIAHII>7;FEI>?CGFIIFIHC><5?<B?<=8<>BABGFACIFDDDEABBDBADBCFFFECBDDFFFFEDECDCCEDCA>ACADDEEDBBCB@<?DI>AAA@CIC	NM:i:4	AS:i:130	XS:i:130
virus|29|0|1-150=AAV_RSV_GFP:245-395	83	AAV_RSV_GFP	246	0	150M	=	139	-257	CAATCAGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTACATGAAGAATCTGCTTAGGGTTAAGCGTT	0/<8<691)87?=BD9>2:5-.;;>IE??>?E<8?@=;B<>@GIGC;D@E@BEDACA?<@ABCE=?CBADCCDG@?FC?ACCDDAEEGDD@BAADEEEDCDEDDDDDCA@@CCABDCCEB?ACFDEFHCECBEEDCCGICC?=ABBFCCH	NM:i:3	AS:i:135	XS:i:135
virus|29|0|1-150=AAV_RSV_GFP:138-288	163	AAV_RSV_GFP	139	0	150M	=	246	257	ATGATTAACCCGCATGCTACTTATCTACGTAGCCATGCTCTAGGAAGATCTCGACGCGTCATGTTTGACAGCTTATCATCGCAGATCCGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCC	=A@BDAEECABCIIIIEHHGDB?DEGGFFBEEEEBCGCDCAADEEFEEDCGFGBFGECDDDCE@>FCCIEFDEF9?CB=IGHD?BIICC:BEC>BB<EC>=C8=>H;991<;99AF<>:D7;66?9@5;199<9<04?@;<?=<G;12B>	NM:i:0	AS:i:150	XS:i:150
virus|30|0|1-150=AAV_RSV_GFP:1420-1570	83	AAV_RSV_GFP	1421	0	150M	=	1139	-432	ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAATGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC	@5::E<1,C=?>+737CE;;899;;3:F::86>E?EIA=<AD<D=EB<8??CF9B7DHBIIEEA?IH?FA?C>@HBC@=;FCEFC@@CCBBAB@BEEEFGGDDFGEFEFEFDD@A@FGEDDDCCGEEGFFDDC@>?<?A?CEAC>>B=DC	NM:i:1	AS:i:145	XS:i:145
virus|30|0|1-150=AAV_RSV_GFP:1138-1288	163	AAV_RSV_GFP	1139	0	150M	=	1421	432	ACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTTCGCCATGC	<B@>@BB?AAB@>CIHFEEEFC?CBBCD@ACBDDEGFCDGCHIDDEBEHFCDDDDBEGBFEABEICDABH??DIEA><=IEEIE;>;IGB@>E?96?G@5AD=;=@=F?:<928:28GE?IDEC9B?A@88;>GD<55;;95874/"6:>	NM:i:1	AS:i:145	XS:i:145
virus|31|0|1-150=AAV_RSV_GFP:621-771	83	AAV_RSV_GFP	622	0	150M	=	523	-249	CACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCCATACAATAAACGCT	78<8:.51)92504.?5778EI8;;9=>C9=;8>8;;9;=DH?@B>5?GE>=3=E<;765??DEB@5;<@D@IHEBCDA>BFBACCDIIEEEAFFDCB@DDEGAECBBCDEDCDDC@BDDDCDBAACFEEDDCABDB?AFDB>@BACGEF	NM:i:2	AS:i:144	XS:i:144
virus|31|0|1-150=AAV_RSV_GFP:522-672	163	AAV_RSV_GFP	523	0	150M	=	622	249	TTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTA	A@?@>CBABGHCCGFDBDDBACGDDCDDFDCIDDEEEGACBCFFCBBA@CCDFCHFFBC@@DDAB??A@FEC@EGGFABIIB=?C==8;AD<A>?@FIGC>=?89>FE>9>?;<A@DF@IGD:HB9@AB6>8>F;AA>-@?C;IH?4>@<	NM:i:0	AS:i:150	XS:i:150
virus|32|0|1-150=AAV_RSV_GFP:646-796	83	AAV_RSV_GFP	647	0	150M	=	545	-252	TAAGGTGGTACGATCGTGCCATATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGT	287A:597780406=</570B9657=23@6=@>?@:?C7@88DD=A<53<::>CIII8<<;;EIF>;>GB>BFCD@DDEA@?>A?BB?D=BB@DCDCIFECDEDEEBDFEDC@AEDDEEEFDECDBEEAGGEEACBB?@BBBB?>;<:=?	NM:i:1	AS:i:145	XS:i:145
virus|32|0|1-150=AAV_RSV_GFP:544-694	163	AAV_RSV_GFP	545	0	150M	=	647	252	AGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGA	9;<>BA?@B>BCDEADDFBCDBCCDCDFACHCDEGFFEEEHFEGGGHGFEEFBCDEGFFIIIHEBGE@:>@;>@FEDA4><7<=C@87467=B7IAEFDDB9?:<015>;:=6E6BE@99@@2;3:;<ABE91:5559=98189C0)78C	NM:i:0	AS:i:150	XS:i:150
virus|33|0|1-150=AAV_RSV_GFP:352-502	99	AAV_RSV_GFP	353	0	150M	=	546	343	TTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATTCGCGTATCTGAGGGGACTAGGGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTC	D:6;BA?A>@:DC?AIHEFFCDFAFEBC@BGEEGEFEFEFEGFDEECECFIGGFAADIHEDBBB>:>EEDABDDD?>@;@>>?;8@IDHG>;5>HDIIHEFEIDII<7=B@@BIBBF9A@:;<8:DC?924:8217?==<9<866DH8;3	NM:i:0	AS:i:150	XS:i:150
virus|33|0|1-150=AAV_RSV_GFP:545-695	147	AAV_RSV_GFP	546	0	150M	=	353	-343	GTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGAC	7887?7=83/6.245:95.4582<:B/+6;><9=A=@@@EGIHDEFA>>A:BE@:FDA?>DBECDDI@A@EHCD?DGC>A?=A@?EACCCCEEBBCAFECEEDDBDB@@BFFC@@DFFDHFIGIHDDDCAEDDCC@CCCBBBBABB<C;@	NM:i:0	AS:i:150	XS:i:150
virus|34|0|1-150=AAV_RSV_GFP:1257-1407	83	AAV_RSV_GFP	1258	0	150M	=	944	-464	AAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATC	69C=B994IIE<3.6:IFF?;754,:<E@DBE;>@:?CC?@C2;8>@B=;9IH<7C63@DAFIIEBABA=@>@@?GEGDAA=CFHFEDDDGBDDEFBCEEFGCDDB@EEFEECBDBBDCDDEFFDDDCDDDEDEF@BCCCB@FA=@AC>=	NM:i:0	AS:i:150	XS:i:150
virus|34|0|1-150=AAV_RSV_GFP:943-1093	163	AAV_RSV_GFP	944	0	150M	=	1258	464	CTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGCAGCTGCTGCAGCGGGAATTCATGGTAAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCTATCCTGGTCGAGCTAGACGGCGACGTAAACC	>@?:?D?A<CBHHHFEDDDBB?BBDBA@BCHDDDBDFECFEDDDDCGHDCDEEEEIFAABC>FED=@AEEBBC=>GIEGAA?@3?95@=C?;:=>A9A@B16764283>EG=5/4:4633:B?;<94<=:8=;908BD5=2@D7AHA>;2	NM:i:4	AS:i:134	XS:i:134
virus|35|0|1-150=AAV_RSV_GFP:1656-1806	99	AAV_RSV_GFP	1657	0	150M	=	1728	221	AACGAGAAGCGCGATAACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAGATGCGGCCGCAGTTACGCTAGGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATA	AB?>?@?C>BDCACBCBCEGGED?ACEFDDGDHEEEEHFDFDEEEBACDCEECGHIFEB>CBCFCHIFCDCC@@GDDEFBIEI?<?@BII??FG@E>1,2828>?:IID?5:??9=A5;49E=68731BH=;=8?=A:42=?CA@5,/=A	NM:i:1	AS:i:145	XS:i:145
virus|35|0|1-150=AAV_RSV_GFP:1727-1877	147	AAV_RSV_GFP	1728	0	150M	=	1657	-221	GCTGTACAAGTAAGATGCGGCCGCAGTTACGCTAGGGATAACAAGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTG	68/B=24.-6*.>667*)(425/:?6E=45=@C??@@><IC?AF98A==1<?<F@B;BEA>;<<599><:BCIB<?;7D??EADG@FDBACBDDEDCECDDDEFEEGFGGHEECBBAB@?FGBDDDBBEHGCCDDCBFDBBC?<>>>:<B	NM:i:1	AS:i:145	XS:i:145
virus|36|0|1-150=AAV_RSV_GFP:580-730	99	AAV_RSV_GFP	581	0	150M	=	799	368	TAACGATGAGTTAGCAACATGCCTTACCAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGAGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAG	HE?BHC=ADBB@CA?BCECDGFHEGFHD@BBCBDDDGIEFEGIHFFEE@ABFEEFED?=@>CCBEFC@??AB===>=DA?@CIGDCC>D:754@<FA?A@41A:6;<=3173@49:2>:<?;;--764507.23(/,6A@DIB;35;=71	NM:i:2	AS:i:140	XS:i:140
virus|36|0|1-150=AAV_RSV_GFP:798-948	147	AAV_RSV_GFP	799	0	150M	=	581	-368	ACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTAAAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGAT	CD:6084=359748C?=@>=.0561):=8DD=09>G@B?EICC@;BC>GEFG?:E;?CGIIH@@DGIIEHDDG<7CCCD?FABABCCEFDEB>CBCB?DBEEEFDDEEEEFEEHEDCEEEEDDEEGDEGEGFGEACB<9?AACDB;@???	NM:i:1	AS:i:145	XS:i:145
virus|37|0|1-150=AAV_RSV_GFP:1144-1294	99	AAV_RSV_GFP	1145	0	150M	=	1340	345	AGCTGACCCTGAAGTTCATCTGCACCGCCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACACCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCAAAG	::>>@BCCAAA@>@ACDB@CDDBDEGGECDDDCDDEDDBCHHDFEEGGFFEHDBBACIFGICF?BBCEEEIFD;;@@@AEC>BCEHIHHIIBIII<7<>@657<3<EF>9:;0;0703BF@8A77:8:/,9840.8<4:8078892)452	NM:i:3	AS:i:136	XS:i:136
virus|37|0|1-150=AAV_RSV_GFP:1339-1489	147	AAV_RSV_GFP	1340	0	150M	=	1145	-345	ACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACA	<D708677>5>B?D:9FGG@:85656CIIH=C>AA;9B8IFFD;=;:=A>E@BDIDD=;AA@@@:B@AEB>@@CEB@?DF?DBA?DEGIGIDGECGHEDCFHEEEEEEDCBFDDGGEDDCFEEFGDCDCEEDDC><BGDFCEFFABDA;?	NM:i:0	AS:i:150	XS:i:150
virus|38|0|1-150=AAV_RSV_GFP:969-1119	83	AAV_RSV_GFP	970	0	150M	=	715	-405	ACATCCACTTTGCCTTTCTCTCCACAGGTGCAGCTGCTGCAGCAGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAG	B7?C?A51;;74';6D@9EI9><IIGE960+:H@EICI?A@:94;7@D?IIF>HCFAC?6@?A:@CIG<=B@CE@AAEEGABBB>:@C<B@BEBADEFHFHFEFIGDDDHEEDFEEFEEEEEEEEEFBCFGCDCBCCCDEBCCCEFA@DB	NM:i:1	AS:i:145	XS:i:145
virus|38|0|1-150=AAV_RSV_GFP:714-864	163	AAV_RSV_GFP	715	0	150M	=	970	405	AACTTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGG	>A==?@B@A?AABHCIDDDDB@BEGIEGEDFIGIGEBCDDCDBCDEHFBBFDGHGFGFCA@B@BCIFC<;?IACBIEIHIEAHBBC>@C7<@@BGCID>=8DI?I77<:A<@EAA?::;0-7;<;99F=7?5;5+:?:;;@G38,-9*93	NM:i:1	AS:i:147	XS:i:147
virus|39|0|1-150=AAV_RSV_GFP:981-1131	99	AAV_RSV_GFP	982	0	150M	=	1107	275	CCTTTCTCTCCACAGGTGCAGCTGCTGCAGCGGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGAT	?C@CC?@=?=<@>DIFBDDEBCFFDDDDIIEEEECBDBCDCDDDFFDCDBEEBEEFEDDDFIFEECCCAA?DAD<?GIFICBBFA:=<>>AECID7HIDE;;609DHIH<6<?DDH;;;7FC</C?>D@>33'/9A0)!"9<//4E@=8?	NM:i:0	AS:i:150	XS:i:150
virus|39|0|1-150=AAV_RSV_GFP:1106-1256	147	AAV_RSV_GFP	1107	0	150M	=	982	-275	CGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCGGTGCTTCAGCCGCTACCCCGACCACAT	527B@;56G=A4;BD>BCE7:11:9C;C@C>@8B<<;BF>GADIIDBB>>;DG<8<@9<=HI=IIG13?9<A@B>>DFIGEIFECFCBAECCBBEEDDFEEGIEEDDCCCBDDCFEEEFFFIEFEDCBBDCD@@@BDDICA=BDEIA>><	NM:i:1	AS:i:145	XS:i:145
host|00|0|1-150=chrX:7729061-7729211	99	chrX	7729062	60	150M	=	7729357	445	GGACTGATATGGGGATGTGAGAGTACCTAAGAGGTTCAGCGATATGGGTGGCAGATTAGGGGAGCCCTGGAAGGGTTAATCTAGAGGTGGAGGTGTAGTAAAGACAGGGCTTGGGAAGGGAGCTGGTAGAGAAGCTGCTCCTATTGGGCC	<??>=<>?@DCC@>BCGFGDDDCDBBCDEIHGEEEFECBABAEHDDCFEDEEFEGFD??BCEFFAB>BAHCC;;;;>AEC>AICEDHAE>??I65;IE;@?AACB=>DC:=CDD>=:>>F730588137909:=IE=520?@<04;=GII	NM:i:3	AS:i:135	XS:i:19
host|00|0|1-150=chrX:7729356-7729506	147	chrX	7729357	60	150M	=	7729062	-445	CTTTGGAAGGAGAATGACACCTGGAAGGTGCCTACATCTGACTGCAGGATGGGGGATCTGCCAGGGTTAAAATCAACGTAGAGGAATATGGGAGCAGTTGTAGGAATACCAGAAAGAAATCCTGCAGATTGAAAGGCAAAGACCAGAGAA	<;7;6748<7<:I9/<HC6>:2@7738?>?B<BC?556?C=E<:,42:DA@I?I=FG@DB<?=B=><73<GB;=<CDCA?BAGGEBADECDDDEFECDCCCAEDBEEEEEFGGEEDEDCDDDCGEHIEFFEHICAB@B?CDB??@?<>==	NM:i:1	AS:i:145	XS:i:21
host|01|0|1-150=chr1:22748956-22749106	83	chr1	22748957	60	150M	=	22748686	-421	CAAAAAAAAAAAAAGTTCTTGTAAACCTGCATGTGTGGTCTTCCTAAGGGCTTGTTTGCGTTTAGTTCACATTTGACTTAGTTCGATACAACAAAATTCTCATGGAAGGCTTCAAAACACGCACGTGTACTGTTGAGAAAGCTAAATATT	D3.6308?;>50:78@8;9518;<635CE8<EE=H=ACGA9;?A@;>=AB<2?GI=@?H>DD@@AIBADECDFIFGGFC@>:@>?CCCCB@GABCB?DDGDHEEFDDCEEDCBCEDCEGIGGEEECFGIG@>D@CA@>=?CCB@CC><7;	NM:i:2	AS:i:140	XS:i:0
host|01|0|1-150=chr1:22748685-22748835	163	chr1	22748686	60	150M	=	22748957	421	TCATGACAAGAATACCACACAGCAAAGGCGGGCTAAGGTGCTCATCTCTCTAGCGTTCTTTTCTTTAGGATCAAATGCATATGGTTTTGTCAACTTGGACATAGATGCATAGGAGTGTAGCACTTAAAACACAGCTAAAAGGTGGAACCT	99=?:@@C??BBCBDIGFHGFEEEDFEABCDDDFDDEFEFEAEEGFCCECDDBCBBECAACBC>?HEEEBIE@@???>@AEC<AABA?B:8=B=:;8@@F=>:=@?D?B@>:?G2?>;DC;HH@>I?FFIB?H7962689:;17969@<F	NM:i:0	AS:i:150	XS:i:0
host|02|0|1-150=chr4:107475626-107475776	83	chr4	107475627	60	150M	=	107475480	-297	GCTGTCCCCTGGGGTCTGCAGCTCTCCATAGGTTGGGCTAGCCTGAAATCAAGCCAAACCTCTGATTTACAAACACTGCAAACCCATCTGCCTCCATGTGTTGGCGCTTTGCTGGGTCTGGGATGCTGTGCAGCTCAGCTCTCTTGGAAC	HEIIAI<;<?BGCII?4H>=@IFB>E==79<?;:4A94646?:?DCHEH;:8DACG7CAG=B?=AEIG>BBEBDIDBDFIICBCCBBDCCA@CCDEDEEECDD@DBEBCBDEGGCDAHGCCDAA@ABDDDEEEEFEFDEBAGFD@==@=;	NM:i:0	AS:i:150	XS:i:0
host|02|0|1-150=chr4:107475479-107475629	163	chr4	107475480	60	150M	=	107475627	297	GAAGAACTGTCAGCCCTGCTGTGATGTGACTTGTACCAGTTATGTCTTGCAGTGTGATGTGATGTTCTTCCATAGTGGTCCCCATCTGGGTCCCAATGGCTGCCTCTGATCTTCAGCCTTTTCAGCTCAGAGGTTAGGAGAGAGCAGGCT	9;?A=?B=AB@@@@>=@DCDFEFFEEEFGFGGECACFEEEEFCDDDDDDCFEFEFED@FHFFEFAFECDD@?I@;>8>>@=:>=@=6=>?<>B6?>7.2=:>9DAAC><FDGF?G=3=B:<;IF;:<2465469B@>B1,?A=C9:;9@I	NM:i:0	AS:i:150	XS:i:0
host|03|0|1-150=chr4:14696464-14696614	99	chr4	14696465	60	150M	=	14696571	256	AGAATGAAACCCTCATATGAATATTTTTTGGGCAAAGGATCACTCAAGAATTTGGAAAGAACTTTACAAGAATATTTAAGTTACTTGATCAAATATGGCCCAAGTTTTACTAGAAACTAGACGTGATTAATTCTGTTACTGTTTCAAAGA	;;8<CCB?CBA@ABCDEACBDDFFDFEDCDDGIIEHEEEEGGCDDDHFEDCFCACCDBDEEDEABCDBE?AEAB:?@AAC=BEFDAC?CFAIA@?979=9:6@5A?E@>:<9877<A<5F@EAABEE@E<;@=85<<7458BBI?AFCCC	NM:i:0	AS:i:150	XS:i:0
host|03|0|1-150=chr4:14696570-14696720	147	chr4	14696571	60	150M	=	14696465	-256	TTACTAGAAACTAGACGTGATTAATTCTGTTACTGTTTCAAAGATTCTTGCTGAAATAGCAATTAAAATGAAAAGCCGTACTTCTGAGGGCTAACAACTCAGTTAATTACAATGTTTTGAGAAAACCAAATTGAGTAAATTGTCATGTGA	/6@:?3:;:1809<4>4:@D>=DEB<D>9AB?;=F;<==:;C?@?>I?ABC><<?E2;@@<DIBF@9?HH<>BD=AB?B>A??HADDFIGHDDDCBDACCADCB@@EGGEFCDCBHCBDHCEFGCDFA@DCBEC>A??@AA??@?@AA?B	NM:i:1	AS:i:145	XS:i:19
host|04|0|1-150=chr1:64467655-64467805	99	chr1	64467656	60	150M	=	64467894	388	CAACTTCTAGGCTCCTGAACTGATGGGAATTGATTAGTTCACTTTTAACTGCAGTGTTTAGTAATGTAAAATATATTACTTTCATTGTAGTGTTCTATAACGAGTAATGTTCATTATAGTATTAATTGGAACATTCAGTTTTGTGCAATA	9<A>???A?=>;><@BCDDCDCDEIHHFDCHDDDEEEFEDCCEEDFFFEBCDFEECDDE@BF>ADCD>;<DFAEIDA<<6<=BDB<46?BIGCA>EICE@9@CDAH<CH?;5ABD=I=,4?CHII?B<;<=:?8C?IGB@<5;=>::;A4	NM:i:3	AS:i:135	XS:i:19
host|04|0|1-150=chr1:64467893-64468043	147	chr1	64467894	60	150M	=	64467656	-388	TCATAAAGTGATAGAGCATCTTATATTCTTTTGGCAGAGACTGGCATTGCACACTCCCTGAGATGCGGTATTGCTTGTGTTTAATTTTGCCCATATCAAGTTGGAAGTGATAGGCAATCTTAACTTATGATATATGTACCCAGATTGAAA	3987,'3033.)04->A6><<32<3=4:EIEI48:>:955;8>B;AI@8E=?8:;@G@?C@BEI?@EB@C=GG@DC>GIIIGDEGCIGBAA>CBEEEFGEDCDEEEDDDDBEFHGHBBDCDCCBBDDCEDECCEEAABAEBAA<>;<?@?	NM:i:0	AS:i:150	XS:i:0
host|05|0|1-150=chr7:63677746-63677896	83	chr7	63677747	60	150M	=	63677565	-332	ATCTTCCTGTGGCCAACTCCCAGGAGGATATGTGTACCTTTTTTCTTCTAATTCCAGCTAGACTTCCGAGGAACCAAAGAAACCATTCCTTGCCCGTGTGGGTTTTTACTTTTAAAACCATAAAATGTATAATGTCCCTTACATTTTGAC	C/9(/5@C<=9<57DH<9898;;96@>==7;:;>=/1:E;7@>AD@8@818BCG;8.,49AC?>A<9;F:=?CAA@??AA>CDFGCF???FDCBCDEEFDEDDCCCDDEEDCB@CDCDFCBDCBDEFHGGHFDEDDEFC?B>=?AB@AD?	NM:i:1	AS:i:145	XS:i:0
host|05|0|1-150=chr7:63677564-63677714	163	chr7	63677565	60	150M	=	63677747	332	TGCCAAAAAGAAAAAGAAGACAGAAAGAACTGAATAGTGTTGAGTGTTCCTGGAGGCTGGCAGAGCCTGGGTCATTTGATGCTGGTTACAGGTCTCCTGTCACTACAGTGATGACAACCACAAGACAAGCAACTGCAGACAAATCTAGAA	>>>?<>>==?EGBDHCDDBBBBEAEFFECDDDCBDFCEDEEHECDEDEGFFCDEGGFEDFBCIIAEDC>BEDACG<BC?CBAA:@=;.:<>>B?@D<<A?DH:9<IIC:BIF??C@B?>7HBFD?EAAGGB><9=>BB@87FF438323D	NM:i:0	AS:i:150	XS:i:0
host|06|0|1-150=chr7:35449032-35449182	83	chr7	35449033	60	150M	=	35448923	-260	CTCTGTACCTTCTGGCTTCCTCCACGCCTAGGCACAAGACATGGCCCTTCCTGGAGCTTGGCCCTACAATTGCGGGCTGGGTGGTGGTCAGCTGAGAGAGTATCACCGACTCAGGTAGGTCCTGCTTCTGTCGAGCTGGAGCCTAGACAG	955:E>71GACGBAFG?46I?>AA;90$5:B96053>?@B=;;<FCF=>47IGEEEACEEIIFCD?@F@@IADFA@FCCDBFEFBBACDE?@CCDDCDCBFGEEEEFGFGBCEEECDEDFDEDD@DDDBBCCEGIIH@BADDDE@B@AEF	NM:i:0	AS:i:150	XS:i:0
host|06|0|1-150=chr7:35448922-35449072	163	chr7	35448923	60	150M	=	35449033	260	AACTTATCTGATTCTCCTATTTTGTGTCTGGCGGTGTCTATCTGGGCTTTGTTGCCTAGAAGGAAAAAAAAAACGGGGGAGGTGGGTGTTTAATAATTACCTCTGTTGACCTCTGTACCTTCTGGCTTCCTCCACGCCTAGGCACAAGAC	?@A@@BAEA??BAFCEEEEEIFEFHDDDABEGFEFDFEEEEEDDDGCCCGBCFDDEDIIGCCC=@=ACCIFD>=EIE=;<C>DBAECEEGEIDGC==5?9D8?EF?=6D@<@<D<A99;:#:359E;C8>A9?A>843-=:468=@@H<>	NM:i:0	AS:i:150	XS:i:0
host|07|0|1-150=chr4:84426118-84426268	83	chr4	84426119	60	150M	=	84425851	-418	CAAGAAGAAATCACTTATTTTTCTTTTTTCAAGAGTAACACTGAATAAAATAATAGATGTTTTCACATCCAAGTAGATTACCTATCGACATATGCACAGTCCTCTTAACTTTCACACAAGGGAAGGAAAAATTCCAATAATTCTGAAATA	;:-9A8;>60*:7@BHE:IBCI>@+)7<>;:?<H<:>479;915>FCDHHA@E9979@7=BCAA@?=;:;@<?:;9@B?9:C>@DADEFFFEDCCEEEFFDDCBDCEFFFEEEFEFEFEEBDEEEEGEEDCDDDDEIBBA?;:@@???@?	NM:i:0	AS:i:150	XS:i:0
host|07|0|1-150=chr4:84425850-84426000	163	chr4	84425851	60	150M	=	84426119	418	AAATAGCGAGCAGCCTTTAGAAAATTTCATTAAACATTCATACTAGGTGATAGTGGTTTTAGATTAATTTTTAAAAAGTAAGCCTATCTACGTGAATAGTTGCATTTACTAAGTTCATGGCTACAATGAAAAGCACGGAAAAATGTTCCA	????@@CBHGIEABADDEFCGGIIHHEEEGEC@ABBB@@ADDCCDGFACDBBCBCCCD=<?AC=CE?AGBFD88BFCIFI:>@IFEHII=IA4:<B=?IFB-22B>>5??>DC5=;@FB:=<@<=A9:FGDHF;8;;4=<;40<;<979C	NM:i:1	AS:i:145	XS:i:21
host|08|0|1-150=chr6:99156362-99156512	99	chr6	99156363	60	150M	=	99156634	421	TTCAGGGTAAAATATGCTTTTTTTTCCAGAAATAGACACCAGAGGAATTAAGAAAAAGGGGGTGGGGGCTGTTTTTGTTGTGGCTGGCTTCTCTTTACTTACCAGAATTATCTAAACTCCCATTTCTGCTTTCTTTCAATTTGTTTGCAT	:9>A?@@A=>ECFIFIHIGIGDDFGFGFFDCDABADFCDCFEFGEEEADBDDEEEDFIIECFC@BACIHA9?GFCGBBFDHIHBAFCE>?@@@@CA7=7:<=B;ADA><<68=CBC8?FCC>=;IG??A>0*1,;?76:63:C0,1=A7:	NM:i:1	AS:i:145	XS:i:19
host|08|0|1-150=chr6:99156633-99156783	147	chr6	99156634	60	150M	=	99156363	-421	CAAAATTAGAATTTAATTTTTTACCTAGCTGCTAAAGAACATGTTAGCTTCCAACTTGTACTGGGTCCCAGTAGGGCACTCTATATTAGTAACACTTTTCTCTACACCACCTTTCTAGAAAGTTCCCTTCTAGAATGCTGAGACTTCATC	23598;B<64)0(0(?97:859<@D17DIGCIEDGFFGF9IC?@ADIDA@F66@9643H=B8/@BCIDHBFIHEAADHGBBGDFGGFHFIGBCDEAACCBACGCEDDDEDDCBBADACDFEACFFDDDCDDGCCABBBCCC?AC????@B	NM:i:0	AS:i:150	XS:i:0
host|09|0|1-150=chr14:114917441-114917591	83	chr14	114917442	60	150M	=	114917315	-277	CTTTGGGGTTTGGTGTAACTGTTTATGGCTTAGCACAATTGCCTCTTGTAATTCTGAGTATATCCTAAGGAAAAGACGTACTCACTCAATAGTTAAGGCTGACCATTTTGCATAGTGGTCGTTAGCTCTCACTGTACTATTTCTGTGTTC	6A;9G6865<5;15==:=A=;C;H?<IB>CC=;:66039ADIGB:>>DBG;@BCGG@DDD?CEGFI8?=BGAHICDBGFCH>DGDDDDGFHIEGDEFHIECDEFFGFEEEFCFFEECBACBADEDGFEBBDDDGEDDA@<9@<<@B@@D?	NM:i:1	AS:i:145	XS:i:0
host|09|0|1-150=chr14:114917314-114917464	163	chr14	114917315	60	150M	=	114917442	277	TCTGATTTGTGAATTCCCTTGTCCATCAGATTCTAGAAAGAGCAATACTTGGCAAAGACGGAAGCTGACATCTAGGTAAGCTGGAAAGGCCTGTTGCTCAGAATCCCAAAGCTTGAGCTGAAACGGGCTTTGGGGTTTGGTGAAACTGTT	8AB?>B;A@AFBBCCFC@@CCDAEGFFEEBBACCDDDDGIGFEDGFGEFIFDDDFEE>:?@CDFDIECCEBBIID@CB:=CIF@D?CA@@A9:EDGIID:<;AE=7:,,8B??G;?:7?E<C>>56694=<<I@:<;D>/31>;1-&014	NM:i:1	AS:i:145	XS:i:0
host|10|0|1-150=chr19:10646891-10647041	83	chr19	10646892	60	150M	=	10646682	-360	AGCCACGTATGAATCAGGCAGCCGCTGGAACCAGCCCGGATGTTCTCAGTGCTTGTGCCAGGTAGGTGTGAAGTCCCCAGGCTGCCTTGTGGGAGTGTCTAGGTTCTGCCCACCACTGCAACTCCTATTTGTGCATGCAGGATGGAGAGG	-;>592/@<5@AE?996<<@915:69=?6:91345B>>98:A<:7:28D:@FAFGDI<II8<?>IF?>85CDDEAA@B?AEGHFIGIEDBBBDFDFFGFEBBEFDDHGFHGFDFGFGCCEDDDFHGECCDDBBDDCCFDD?@@?;A@@A@	NM:i:3	AS:i:135	XS:i:19
host|10|0|1-150=chr19:10646681-10646831	163	chr19	10646682	60	150M	=	10646892	360	AGTTAGAACAATCAGTCAGCCATCTACCACCCAGGTCCTACCCACATTCTTTCCCACACAGTTGATATCCACTCCAGTGCCAAGTTCCTCCCCACTGGGGACCCTTGGACCCCCCTCACTCCTCCAGGGGGCTGTAGGGACCCCTTCCTT	?>>@B@C=GABBDE>BGDEEDDDBECFEDFEFEEDCIIFFDBBCFFEE?CCEFEEFE@EDDBGDBDCGCAI@FE>7>AIF=6?@B8;:3A>=@A=DGA?EIF@H;@B;8;BII<E;G?>;>F69;4>9EEI6F<;GIC<;?35421<@EG	NM:i:1	AS:i:149	XS:i:0
host|11|0|1-150=chr18:84376768-84376918	83	chr18	84376769	60	150M	=	84376667	-252	TACTGACCTCTGCCAGAGTCCTTCTCTGCCTGTGACAGCCGAGGGGCTCCCTCCTACGTAAGGCTACTCTGAACTTTGTTCAGAATGTTCTGGTGTTTCTAACAACTTGCACGTGCCAAATAACTCAGAAAAAATTCCAAATGATGCTGA	;=:;@B<;:840;<4>:9.8A8024BID>9IA@B@EC96=B=;7<8@::<<;7<@69DID>?>ABCC@FA<B@@>C:8>BIGEABBDDEBA?CDEEBFECCBBABEEB@CCEFGEFGFHGIGCFEEFCEEEFGGGGFDDGBCBAB=ABA<	NM:i:0	AS:i:150	XS:i:0
host|11|0|1-150=chr18:84376666-84376816	163	chr18	84376667	60	150M	=	84376769	252	GTGCAGACAGCTGTCAATTCTGCTGAAACATGCCCCTAAAGGGCAGGTGAAGATGACCATGCGACACAGCCATGTGGCTGCAACCTGTTTCGCGTGCATGCATACTGACCTCTGCCAGAGTCCTTCTCTGCCTGTGACAGCCGAGGGGCT	A=;:?>@>DCCABE?A@>@ACDEFEEEHGFEEDGHIGHFEGHFHGEEEEEDDDBC??BC@BDD?BC@A=>>EC=?<<>C@AI@:DE9AB=@>4CCD;4:;HC<:6/9<?F949><=<7<52%*(&*66)<B6=/92<=>>6-/+.4<6I=	NM:i:1	AS:i:145	XS:i:0
host|12|0|1-150=chrX:55638399-55638549	83	chrX	55638400	50	150M	=	55638111	-439	TAGACCTTATATTTGGTAGAAAATAATGAAAAATACTTTGAAATACAGTCAATTTTCTACAATAAGATATATATGCGTTAGAACTTATTAAATTTTGATAAGCTAAAACATAAAAGGAGACCTCTCTGATAATCTTTTAGGGAAAAATCT	E.7-?56*;F>>7())?C92B:9?BB@<2;;A8:@<;>8;BI@7;=<768-91=;D>=<GID@A=>?BDDHFIGFBFFCHDEGEIIDDDEG>CDDDCCCABACFGDEFEFFCCFFEIEEDDEDEEEHHFGEECA><<?DBBDBB?>:<;8	NM:i:1	AS:i:145	XS:i:135
host|12|0|1-150=chrX:55638110-55638260	163	chrX	55638111	50	150M	=	55638400	439	CAAGGAAACTAAATCTAATTCTTAGAAATCTGTGAAAATTATTATTTCCTACTTTAAGTAGCTGTTAATGAGATTTTAAAATTTGATTTACATTCATTGTGGAAACACAGAAATCTTGCACAAAATCTTAGAAATGTAATTTCTGATTTA	:A@>AA<BA>BBB>=@?CCECDDCEHEEFBFECDDDEEDEGFBAEDAACDADA?DDD;BA>C?><:?A<IFBB:@A>AAB@BFIIA@IIC?9,35;3E=:?B?;8<AC:49:28?BG=27@@>B758A:+6FAADFEG9B87;H@7;5;G	NM:i:0	AS:i:150	XS:i:140
host|13|0|1-150=chr16:88636621-88636771	99	chr16	88636622	60	150M	=	88636880	408	TAACAAGCTGAGCCATCACCTTAGCTTTCCAGTTTCTGTTTTATTGTTGGCCTTGTTTTTTTTTAGCACCAGGGCTTTTCCTTAGATGCCACATATGTTCCACAAGTACTTGATCACCGGGCTACGTCTATTTCCCTAGCTTTTATATCT	?=<@=EDAHED@=@AEEFFFDEDDBCDEFHGHEGDDBCBCDDDBCAEFEDDGGIHFEEFCC@GEDGCAC<B>BGEFDA;<9=@<D?>?::==::;C;??496;C:6C528IGIG=9.57E<5676134/C79=<G89H38</:8>AA;D0	NM:i:1	AS:i:145	XS:i:0
host|13|0|1-150=chr16:88636879-88637029	147	chr16	88636880	60	150M	=	88636622	-408	ATTCCTTATTGTTGTGCCATATGTTGTTTTGAAAAACGACATAAAGACTTGATGAAGAGCAATGATGCGCATAAGTGTACCTATCAGTTAGTGGTTCACTCGCACAGTTTTAATCGGATCCAACAAGGCAGTTTAAAATGAGGATGTGTC	9C;B:87/2574B?A8<89<>A87>;;;=45A@BD58B@8990;BHF9ADEGIIGA<?;6DCB@IHC;CGBBC>;>>>ABCBDGC;>ADEDFDFBEEEFIGEECBCDDFHFEEEDDFGIDCDEEECFCFGGEBFGBB@CA;?BDCC@ADB	NM:i:2	AS:i:140	XS:i:22
host|14|0|1-150=chr2:133795179-133795329	99	chr2	133795180	60	150M	=	133795446	416	GATAGATTTCCAGAATAGGTTTAAAGAAGGAGTATATTCTAAGGCCAGTACTAATGCTACCAGATTATAAAATTGCCCAGAATATGTAACACATTCAAAACTCCATGACAGGTGGGTATTGGGGGTTGGGGACGGGGACAGATCAAACAG	>=>=AE?>>ACDFD?CACCHFFEEDDDDDAADBCCEDACDD@ABCA@ACDDBDECCCBGFA>ABABHFGE?EDC=<CAAB>>B::0-:>?79B;9>8<?===?>B??;<<611446:<=8958611<<-0,,/<8:9387CCA?2=:638	NM:i:2	AS:i:140	XS:i:19
host|14|0|1-150=chr2:133795445-133795595	147	chr2	133795446	60	150M	=	133795180	-416	AACATCTAAAGTCTCCCACTACACGTCCCTCAAATTTTCAGTCTCCATGACTGTAATGCTCCCGTGGACATTCCACTTTAGCCATCCCAGTGACTTGCCTTTTCCTTAATCTGGAGGGCTGAAATAGTTACTGCTATTGCTCATAGGGGT	1.>>D86<>B@@=GEI;;;;9;;;:B:;BG@@4*79;:5>H<:3A;;CCC=;355B6D=8:B=:<=@BDC@DBIFED@EEGEDEGCCCBFEED@?DCHEEECEEFGFFCBDCEEECCCDABBFGHEEFGIEEEGIG@8<<<=><A@@GBF	NM:i:2	AS:i:140	XS:i:20
host|15|0|1-150=chr2:96164703-96164853	83	chr2	96164704	60	150M	=	96164566	-288	AGAATTAGCAGTGTATCACATCTGTACACTTACAAGGATTGAGGGAGCCTAAACTACTTTCTTTATCCCATTTCCGAGTCGAAAATTCTTTCAAGAGAAATGTAATCATTTTGAAAAAAAAAAGGCATCATTTCTTAGCAGCCACAATAA	62-/@FA@GIGE8DG>C<A460485ID8;AI?A;<B<477;46:;FI=IIIIFHD;99A@HB:EH@:<=8BD=CDFICIA@BBA?DA>D??CEFDDGCACDEGEEEFGGGDDDEDEDBBDDEEFHEAABB@CCFFDC>=B?=BA=?:<BA	NM:i:0	AS:i:150	XS:i:0
host|15|0|1-150=chr2:96164565-96164715	163	chr2	96164566	60	150M	=	96164704	288	GGTGTTCTAGATAAGAAATGATTTGTTTCATGTTTCTGACCCCTCCCCCCCACTCTCACTCTCTCACTCTCTTCCCCCACTTGGCTCCCAAAACTTTGAAACAATGTATTTAATGCTTTGATGCTGTGTCCTAAGGAGAGAATTAGCAGT	:9<A@:>?@?C@A>@BCDDDEEB@BHDFEEEHCBCHGGGE@DBCGFECEEEECCDCCCC@ACDDEDCEF:;?@>AE>>A:==@@DEC?C>D?I=G>FCAII789A7::926CIDA?>59373@AC;;:;AD@5=?896=<ACCA4>5/45	NM:i:1	AS:i:145	XS:i:19
host|16|0|1-150=chrY:35713645-35713795	83	chrY	35713646	22	150M	=	35713477	-319	TGTTTCTTGTGCAAGTCATTAGAGTGTCAATTAAATCTAGACTTTGGGAATATATCAATAAGCCTGCATTATCTTCATTTTCCCTGCCCTTAATTATGGTAACCTTGCGAAGAATAGATGTCTCACCATTCTCACTGACAGTTTACCTGG	:;>4,85E<5=6@BDA@I=EAB7:A?@5:=;BIHGED:6;94D;>?;7?::5159;B?DIFIC@:AAIB@EEHEIEEICBDIFFEHIC;<??@ACFDDDFEEEEDB?FDFDDDCCABDDDEEEEFEEFEEFDCDCCCBBCBEBEAA@@@@	NM:i:2	AS:i:140	XS:i:140
host|16|0|1-150=chrY:35713476-35713626	163	chrY	35713477	22	150M	=	35713646	319	CACTCTTACACCATCTCCTGCCTTCACAACATACTTATTAAGATGGTGAAATATTGGGAAAGCCATGGTAGAACAAATTCTTTAAAGCCCAGTGCTGTTCTCAACAGTCATCTCCTGATTTTCTATATGAACCTCATATGTTACACTTTG	CABA@CC@=<@?=?B?BD?@FEEGHHHDEDBDEIFEEFHIHHEFFFEFFEEFDDDHCFE?>A@BD@DADAAHIAGHFEECGHCBFGEG@CA@@>?9368:A@I@=9>.<FGC>DEAEA@7<:54=>;?C=C=1=154/;>BI@<;;>97:	NM:i:0	AS:i:150	XS:i:145
host|17|0|1-150=chrX:12388619-12388769	83	chrX	12388620	60	150M	=	12388544	-226	GGCCAGAGGCAATCATCCTGCTTCTCCATACATCAAGTCATTTTCTTGTTGCCTTACGTCTGCCAGAAGCATTGGATCCTGCGAGGGCAGGAAGTGAGCAGGGATACGTTTCCAGGTTGGGGCAAAGGCAAGTTGGCTGAGGTGCAGGTT	6.(76=6:68:<C<;6=.GDD>9>?>3.I?6?=8FD;>?AI?D<9:CA=AC8C@777G=9CA><BIF?D@B>=CC=IHIHGCDGCCCCCACCBBGEEDEEEEEGDEEBEEECEDDCFFCCCBCDBDDDEEFGCEFEFFDHC?BC@>=@=@	NM:i:0	AS:i:150	XS:i:0
host|17|0|1-150=chrX:12388543-12388693	163	chrX	12388544	60	150M	=	12388620	226	TTTGCACTTAAATCTTAAGGTCTGCCTTCTAGGAGAACCCATGTCTGTCCTTGTATAAACTCAGACAATAGACGAAGGCCAGAGGCAATCATCCTGCTTCTCCATACATCAAGTCATTTTCTTGTTGCCTTACGCCTGCCAGAAGCATTG	E>>?AACB@BC?CHIIEEDBCDFEDCDDDDEEEEEEADGIGFGDDIIGDECCFFFGCEEGFECCBEDCEIIEDCFD?;>@A=5CIG>:<CEB=?9@BHB@9996IC;:;<@?>554=FF?@FIIIA<:/66B>47;@?46@-):::=::8	NM:i:1	AS:i:145	XS:i:0
host|18|0|1-150=chr14:69347275-69347425	99	chr14	69565526	0	150M	=	69565669	293	AAGAACGTGAGCCAGGCTCTCAGCATCTCTTGACTGAGGCCTGTATCTCTGGCAGCATGAAATTGGTGTGCACGAATGCTGCTCTAACCCAGTAGTCCCCACATAGGATCACAGTCAGGTGGTGTATGGCTGCGTTTGAGCATCTACTTG	@>@@>?@@FFEIEIF?BDBBCBCDED@BEFDDDBCGDCDDAEEHFEEHCBDEBDCEEDCHDEDEGFDDIHGIFBE>87;<9<?FI@8AB@B<-39ACE8;GE9=;:5557?EIEG@>D=;<<B>@5:3>25-.(>B=G=CE@C:E?:93-	NM:i:0	AS:i:150	XS:i:150
host|18|0|1-150=chr14:69347418-69347568	147	chr14	69565669	0	150M	=	69565526	-293	CTACTTGATAAGGCCATAGATATTTTATTTGATTGTCCTCTTCTTGATTTTTTTCACACTGAACCAGGGTGGCCCTGACTCCATGAAGGCCCCAGCTCTGATTCTGTCATTGTGGATGACGAGGACTGACTCCCACCATATCGCCTGGAC	89E:9@CB/<=C8?EB;I>I@6;3;<19:7>H?80,@B?8?CA=1@AD?::=;F?GH<<8C>4=:?>I9=>>??@?=EHGIHDA@DEHIDDDBAEDDEFCBBEFEDCABEGGEDDFDBDDCBDDDDDCCABDC?B?AD?>>A@?>@<:>9	NM:i:0	AS:i:150	XS:i:150
host|19|0|1-150=chr13:36409130-36409280	99	chr13	36409131	60	150M	=	36409501	520	ACAACTTATTGATATTTCCAGTTCAATATTCACTTATGTATGAGTCTTTAGTTCTTCAGAGAGTGTGCCATTTAAAAATTTTATATTTCTTCTAATCAATGACTCACTAAAGGGCTTATAGTAATACCAGATTTAGCATAAGTCAATTTA	@?9=>???@ADCEDDFFCDDDDCDCEGEDDCDEDDDDDFFDBCFEDBCFEDCEBCDDGEADD?A<DAEB>A=AD<;=;>@HII?E@C<=CDBBIEEA>IHEIIC=GCGDC@BA;;=EAI.@;=5@56@?830<997;5?57A8==36D?A	NM:i:0	AS:i:150	XS:i:0
host|19|0|1-150=chr13:36409500-36409650	147	chr13	36409501	60	150M	=	36409131	-520	GAATTATTAAACCAAGAATAAGTGGAATAAAATGGGGGACGTGATACATTTGTGTTTATCTCATCTTTAAAGCATAATTAAAAAATAATCTCTAAATGATGGCTTATCATCCCAGTCCCTGATATTAATAATTAGAAATTATAACAAAGA	?<23GA?9;:>;<C98->A=8DHGB?85.5<B6DHGB<CH47IDFFE95798597<56CCEDA9ABCBBAA@@>CDGHI><FGFCCDBAC@EEDEFEFHGGEFHGFHIHFGFEGEEFFHEFGEDGFGGDDCDEEECAAEFBADCCAA@CA	NM:i:1	AS:i:145	XS:i:0
host|20|0|1-150=chr3:138052247-138052397	99	chr3	138052248	60	150M	=	138052321	223	GTCCAGACACTTCACTCATACGGGTGCTTTCTCTTTTTGTGATTCCGAACTTATTTCTAACCACTTGCCCTTCAAGTTAGACAGCAGGTGTATTTCTAAGAAGTTGTGAAACCAAATTTTATTTTTTAAAGATTCCTATCATTATGAGAT	?<A>BCAACFDCC?ACCFEHFHGGHFEEFIGFDDDBCDDCCEGGHGGHD@FEDDFDDFHFFFECE@B?A>C>;>:@AB=8=BBAAA?@E?F>@C4GFCFB@B?DA>I?;BBD=@?7<<<II>8:=7?A7/56707>EEA10126=7100:	NM:i:1	AS:i:148	XS:i:19
host|20|0|1-150=chr3:138052320-138052470	147	chr3	138052321	60	150M	=	138052248	-223	AAGTTAGACAGCAGGTGTATTTCTAAGAAGTTGTGAAACCAAATTTTATTTTTTAAAGATTCCTATCATTATGAGATTCTTAAAATGTGCAACAATGGTAGTTTGGAAAATAAATATATCCCTTACCACACGTCCGACAGCATCCAGCAT	:9774:385:CAC48.54':53:,.8>9?BB<<FC8=::=@D=38;EEGD<:8<<EHF9>D=8>C=69@EI<C<A@?;A<DIE>=A@;>C@DDFIIGEEGFFEFDGECDCB?ADECDDCCDCADDCBEGFBEFCHIIEGFECADA@?><?	NM:i:0	AS:i:150	XS:i:0
host|21|0|1-150=chr6:4621568-4621718	83	chr6	4621569	60	150M	=	4621363	-356	GACCACTTTATGTTCTCAATCCTTTATTCAAGACACTTCAAGATTTAAACAACAACAACAACAACAGGGCGTTTTTATGTCCTTATGGTTCTTACTCTGCAGTATCCAAGGCACAGATTAAGGTGACAGATGGACAAACTGTATGTTAAG	9D=84/25;FA=8>;D347<EIGIDD<;=>@DBAADA;85DDE=95ACGDA6:85B=768<;;1@CFD@?9?BBCDGCC><EBBADB@?CBACEFFECDCDFDCBC@CABFGCCGFCAFHFDEEEFEEFGEDCA<@DAABBB@?AA><@<	NM:i:1	AS:i:145	XS:i:19
host|21|0|1-150=chr6:4621362-4621512	163	chr6	4621363	60	150M	=	4621569	356	GGCATCCTTTGTCTCATTGCAATTCTAATTACTCATTCCCTCTAAATGTAGAAATAATATTGCTTTCTGTCACCTAACTGATGCTAGACTAAGAAATTTCTTATACTCCAAAACTCCATGTAGCAGCAGCTGTGGTAGGAAAAATAGACG	>>=>AB:8<@EIHGDFBEFC@BDDEEEFEFEEGFBDDDDCFEBCCHFEFEBBCFEDEABCB?=>?ABAA>:@CD<@;8<AIA<>;IIFCG@>@7)699;E<ED>><HID>8:C:G246ABI=;<9:ADHB@9>@?5453)/>7965A>/2	NM:i:0	AS:i:150	XS:i:0
host|22|0|1-150=chr1:161339159-161339309	83	chr1	161339160	60	150M	=	161339045	-265	TGGTATCAACTTACTATGAGTCTATTTCTACTAGCGAGAATATGCATCCTGGGACTCTCTTCCTCTTGTTCTGTCTGCCATGACCCTACCCTTCTGATTAGAATTGAAGTGGCATTTTCTTGAAAGGATGGCTTCTCTTTGCTAATGGAA	IE:?EC<;FFIIGH77>A1/.%)+6?B=D=?B?AA<:@CA??AB5=GCE@7/=4413=26DG@?<?;>>>;<?CCC@>>:<@A=>@CE?=CFDCCBEDDFEBCFFFFFHDDFDC@@CBB@BDDDCEGFHEDCCFDACB?>BDDBC?B?9<	NM:i:2	AS:i:140	XS:i:0
host|22|0|1-150=chr1:161339044-161339194	163	chr1	161339045	60	150M	=	161339160	265	AACTTGATCCACTTTCATGCAATCACGGTTGTCTCTTACATGCAAATGTGACTTTTCAGTGCAACTTTTGCCTTGCTGTCTCCTTTAACTCTTAAGATCCTTCCTTATCTTAACCTGGTATCAACTTACTATGAGACTATTTCTACTAGC	>4?>??CBD=ABEHIGEHIGEEGFCDCDDCBDHFEDFFDBC>BADHEDDCEFBCBEDDBFGF@@;<;BE@>;EC96;8=DE=IFIEDF<>;>@;68347B???5@E:25==7C=<AEE>@BIBD>DB34E=:54'479589475:76845	NM:i:0	AS:i:150	XS:i:0
host|23|0|1-150=chr15:55541344-55541494	83	chr15	55541345	60	150M	=	55541171	-324	CCATCCAGCCTCAAGCCATAGCAAATAAGTTGAGCACAATACAGGGACCCGTAATAGTGTAAAAACACACTACCTATGAAACAGTGTTAACTATTTCCTAAGAAGTAACGTCTGCACGAATGATTCAGACGGCAACTGTGGAACTTCTAA	<@;74<EE<1@FD;@A=9585,:87<87I@@C9:GGC@<GI=>?@9>C<HCIC>AFIB=@B@?;8?DIDGD>CEIDEAA7A@CAD@BCBBACCCDDFEEEFHGCDDDFDCEHHGEE@DBCDCCBCBDFDDDCDDA>AA?:6=>A?>;;8<	NM:i:0	AS:i:150	XS:i:0
host|23|0|1-150=chr15:55541170-55541320	163	chr15	55541171	60	150M	=	55541345	324	AAAATTTAAAATACTTTTAACAAACCTTATCGTATCAATATTAAAGTTGAATTGTATTGCTTCCTAGTGTACAGTTAACGTACCCACATGAAACAGAAACTAACTTCCCAGACTTTTAAAATACTTATAATTAAAATTCTGATTTGTTGC	?;=<:AGCEFIIFCCDEHFEEEDGHHFIIGGCDCFEEGFGEGEDBDCDDEEACDECCBBHEDDBACB>A@?A@EHCBBAADA>ED?<@?9<A?A@HBIDBB<;=I;?717<<=D;??>-602:8@?93-7A>B?=H7;/59C240EEGAE	NM:i:0	AS:i:150	XS:i:0
host|24|0|1-150=chr6:77751216-77751366	99	chr6	77751217	60	150M	=	77751460	393	ACTTGAGCCTCCCTGCAGGCTCAATTACTTCACCATCTACTCTCCTTTTTCAGCATCTAACCTTTGCCCTCTTCTCACCTCCTTTGTTTATGATGTGGACATGTGGGCATGTACAGTCATTTATATGCATGACGTGTAGACATGTAGACA	BAB@A?B@A?@B>>FHDCCDEFFCBAB@DDDE?@DBCDGFACDCDADGFFDFDDDDDEEA?@B?A?@=?A?EAA=?ACGD?@=@B=87;E?I>>E>=4<??@DB?CA81.6683869@2B.:;;<;B5-:?C@E76+?GA9ICIA5=/15	NM:i:1	AS:i:145	XS:i:0
host|24|0|1-150=chr6:77751459-77751609	147	chr6	77751460	60	150M	=	77751217	-393	CCTCCACATTCTATCATTTTTGCTCCTGTCTTTGCTGCTTTCTGAAAATTCAATTTTAAATCTAATAGATGCCACCTTTCTTGCAAAATCTAAAGTGTTCATGGTCTGTAGTGTCAGAAAAAATCAGCATGTTGACAGCATTTTAAGGGA	=CD@752102@37B:9?;<75.8>B=59=I@=93;;:;<AIGBE;G@C7>>96<BDIB@BCA>CD?AFIDFA<=AAAAEFBB@==ADFIGB>BDDEDDCDDBDDDDCEDDFFHDC@BDCFFGFGFFEGDDFGDBEGIB;<@>>CD?B@B=	NM:i:0	AS:i:150	XS:i:0
host|25|0|1-150=chr7:136832912-136833062	99	chr7	136832913	60	150M	=	136833259	496	TAAAATTAGAGTCAACAGGGTAGTGATGAAGATGGATCCACAAGCAGGTGCTTACACAAGCTGCTAATAAGATATACAATAGGCACCATAGAAAGACCTAATAGCGGTAATAGTGATCTCCTCTGAGCCAGACTGGCACCAGAGTTTGAA	<=:9/8<9>>>?@GIGFEDEHECCDECEEEIGGFADDDDIGIGHIDCDCDBCEEEDDBFEEEDDEBBACGC>7=BC?:??BBBD<B<==?95@>;B>==?86@?:<DCDIIID=88:;E9/669<?IH>CB851038>6<;?AB07:6>?	NM:i:2	AS:i:140	XS:i:19
host|25|0|1-150=chr7:136833258-136833408	147	chr7	136833259	60	150M	=	136832913	-496	CCACAGCCAGAAAGGCAGGAGGGAAACAGAGACCCCAGAACTCAGCCTTCAGAGGCTAAGTGGGGGAAAGGAAGGGGAGGGAGAAAGAGGTCATCATACTCTGCAAACCAGAATCAAGGGACAGGAGGAGGGTGTGGAAGCCTGTGCTGA	74,5IDBIDGBFB?:=@H>CCD>?<EA=?::>G9;;>C;856?;.7@?C?CG?8E;13II@@8=@@;>BFH@?=:>;=DDADCDCDEEEDADFDDFDCEFFEHEEEEFHIGDCBCB@DDCEBCEGEFFHCD@CBCEBCGC@C@=;;??=<	NM:i:0	AS:i:150	XS:i:0
host|26|0|1-150=chr1:32153882-32154032	83	chr1	32153883	60	150M	=	32153747	-286	AACTCAAATTTCTCATTTTCATTTGTTTGGGTGTCAGGTTATCTACTCTGTAAAATGTTGGTAATGCCTCAACGCTGTTACTTAGTGTTTTTATTAACTCTTTGAAAACTCTATATAATGTGTTTCCATCATACTTACTCCAACTTCACA	823852+:G7@7402@B@H>=AC7HAI;788*615=B<:9<6:@>9<CA>FB>8HDH>3:@CICFBD?=<IDBCE>@>>EFFCB=?CFDEDECCDECDECDEBCECBCAFGFDDEEEEECEEFCEDDFHGEEEAB=?AGCA@<DC;?@A?	NM:i:0	AS:i:150	XS:i:0
host|26|0|1-150=chr1:32153746-32153896	163	chr1	32153747	60	150M	=	32153883	286	TTGGTCTTCAAATCCACCTTTAGAGACATATCTAAATATTGTAATAATCAATGACTTTTTCATTCTTGAAAACCAATCTCACTTTTAATGAAAGGAATATCTTATTATATTCCTCAACACATTTGCTTACAATTCTAACTCAAATTTCTC	?;A?=99>@>=>@?ACDDDF@CD?BBDDDDBCDCDCDEECEDADDEFEEFGGEFHFEEFCCHFCBBBCDFB<?=<A<:?<8=??=D;CDDAGFCHIIG8=27A945)4:>B>>=4@>=9;2=7?>=:12>7>AHAE?:819643.6C>:@	NM:i:0	AS:i:150	XS:i:0
host|27|0|1-150=chr13:9914880-9915030	83	chr13	9914881	60	150M	=	9914812	-219	AGATGGGGGTTGGGAATAAGAACAGATAGGATCAGCCCAAGTGAGTAGGGGACAGAGTGAAAAGGAGAAGGAGAGTATCAGGTCATTGTATAGATGAGCACCAGCGGACTAGGATTTTGAGTTGCTTATGACACTGAAAAGCAACTGTAA	356A<@:?B;402BE9IBAEIA;99=G2958C<7A>A;F7;48;7?<;796@CG6;><8@C>:@@:@=:879<<89:A?CB=BBBDFGAGCEADFGECDCCBEGEIHDDDDDC@BBACBEDDBEFCDCFECCBCABFGBBA@BBBCAAA=	NM:i:1	AS:i:145	XS:i:0
host|27|0|1-150=chr13:9914811-9914961	163	chr13	9914812	60	150M	=	9914881	219	GACCTTGTCTTCCACAGCTTCTTCTCATTCACGGTTGCACAGACAAGCTGGAATGCTGGACAAGGACAGAGATGGGGGTTGGGAATAAGAACAGATAGGATCAGCCCAAGTGAGTAGGGGACAGAGTGAAAAGGAGAAGGAGAGTATCAG	<=?@FC@>@AD?BFDEEEEECDDDD@CEIIDBB?CDCDDBCABEDHDDDDACBDBBC@DDFDFAD>CFCC?;9;:AFICEDA@=;4:>AAAC<9?<><:@CC???A?;64856CBIB699?.16B:EE./=.6;C:6:E:BD?7>55H;:	NM:i:0	AS:i:150	XS:i:0
host|28|0|1-150=chr15:83435388-83435538	83	chr15	83435389	60	150M	=	83435232	-307	TCCCACATCTATGCCAAATTTCTAACAGCCTCTATAAGAAAATGCACCAGCACAAAGCTGAACTATCTTTGGCAAAGTTTTTTTAAAAAAAGGTAAAATCTCTTACATAAGCTTCTTTGCTTTTCTGGCAGTTGTCCAATCATGGAGAAG	BG??9<IBD75@40A?=;>G>87AF?7?:8CI@?C<><;9;BE18:BB@BG5=927=:5><>8FB;??>CDBC@ACB;<<BEEBGEECGEFDEFDCEDDEDDFGC@BBDCA?CBBCGGCABDDCDCDIGIFEDECDEB?BBB@>B??<::	NM:i:0	AS:i:150	XS:i:0
host|28|0|1-150=chr15:83435231-83435381	163	chr15	83435232	60	150M	=	83435389	307	GTAAATATTCAATTTCAGAAACAAACTTACTACTAAGTAAAGTCAAGTGGTGGTAGATCATTTAGAGAGAGGAGGGTAGTTGCTAATTTCAATCCTAATGTTAGTCTTGCCTATTTTTTAGGAACCAAGATGTAAATGAGTCATCTGGCT	>A>BEFD?@BD@@BCDEFDDDBCDDDGIIGGGFEGGE@EBCFCBBDEEFFHEGEEFFDFFBDIGABBB<AIF?DC:78<CC:=>@88958.,>IDF>>@EA?@D=B:7/956:61838;DD=DHDC-<FA@8:G=>@HE@87CF@HE<B<	NM:i:0	AS:i:150	XS:i:0
host|29|0|1-150=chr19:52174602-52174752	99	chr19	52174603	60	150M	=	52174744	291	TTTGCAAGCAATAACTTTAGCAATCTATTGAAATAGAGGCATTTGTCACCTCATTTTTGCTGCTAATAAAACCAAAGTCAAGATGTTAGGGTCCTGGAACTTGTTTTAAACACAGGGCTTGAGAAATAGAGGCAGAGAGAGAGGGATGAA	6??;=?@@BBA?BA@ACADCEGFACDEEDBEDFCEEHEACEFDABEHFCGIGECDEBDGGDA<BCD?>IIE<9BIED=?@FD==G;;DGEAEDDA:>>97=>3A;0.24<D@6;<9;<?:;::9469@;GI=669:;;98@E@:::6=IA	NM:i:0	AS:i:150	XS:i:0
host|29|0|1-150=chr19:52174743-52174893	147	chr19	52174744	60	150M	=	52174603	-291	AGGGATGAAGGTTTCAACGTCAGACCATCTGAATTACATGAAAGCAATTATATAATAAAGAATCTATAATAAGTGAGGACACCTGAACAAGGACCCAGATTAGAGGATTATTGTGATCATGTGTATGATCAATATATAGATAGAACACCT	04672/7>0<=1(8::+.9CCDAF8;/,83?I?B<A<==?C7?7=>>;2?H=;37>CFB:DBHDHAB;?:9EBC@@>ABCBECADECC?DIIHHCDDCBDCCFEBEFCEHEFCAA>?BEFGBDFCCCCCEFEDDC@ECCBAA?=DEDB??	NM:i:1	AS:i:145	XS:i:0
host|30|0|1-150=chr4:103984825-103984975	99	chr4	103984826	60	150M	=	103985336	660	TCAGAGAAACAGACAGAGATGCTGGTTGCTGTCATGGGAGAACTGCATACAGACCATGCCTGAAGCCCTCACACCCCCCAGCAATGAGGATACATTGAAAGTCAGAGAATGCAATTGGACAGACTTTCAGAAAGACCAGTCCATTCAATG	?::9;@>CBGFCHDDFHEFCDDDDCDDGEFCECDCDFEGEBACHEDDDBCEFBABC@EBCFFHF@@<=DIEGDCBADGBGFD>>AIAA=C=??<HIG=A8H;C744:AC8@FIB@515;=IE;:B95378:G<806<::4".4>ACB3'(	NM:i:1	AS:i:145	XS:i:21
host|30|0|1-150=chr4:103985335-103985485	147	chr4	103985336	60	150M	=	103984826	-660	GATGCAGTGTAAGACCAGCCAGCCTTGCAGCTGTTATTCTTTGTCCCTAAACCAAGTCATTGGAGTCATTGGAGTCACCTCCCAATAGCTAGCATACTTCAAGTATGCTTGGGGCCATCATTACAGGCTGTGTCCTTTTTTTTTTTTTTT	A=A862;<=GGD>9IB?CBI<<@79808=@9:<<<6B;7:9;9?AGE??F@><EI8?>8>EAAH:<;CII?@@DCFHC@BA=>AD@C@EFECFDEFCDCDDDHEFFDDDFGCFDBBFGFGIEECDFCDEEECBFGFFFFIHDIE@?=<>?	NM:i:0	AS:i:150	XS:i:0
host|31|0|1-150=chr10:78421251-78421401	99	chr10	78421252	60	150M	=	78421470	368	GTGAATCTTGTGTGTCTGACTGACTAACAACCAGGATGTTTCATTATTTTACACATTTCACAAAAAAAGGGCAACCTTAAGATTTCACAAGACATATAGATCATATCGTTTTTGTTCCCGGACAAATGTTACAAATAGAGAACGGTCATT	?@>C@BAA?CAAAAHGCBEDCDGFEFHFEFCCCEEDCDDABEIGGGFEEEEDDDDA@C?BCDEHIFCC<?EIFBCDI@<9>DB@>8?B>=?A=@?=BIB@?:F>.9AD@@BC>9;GIIDIC5348/0;2/.54893<34:8=C>7=85;C	NM:i:2	AS:i:140	XS:i:0
host|31|0|1-150=chr10:78421469-78421619	147	chr10	78421470	60	150M	=	78421252	-368	GTGAACCATTAATCAGAGAGAAAAACATCTGAGTCAGTCTAAGCGAGCTCCCAAGCCTTGAACACTGCAAAGTGATTGAGGTACACCTGTGAGTTAGCCTGGTTTAAAAGTTAGAAAGCTCTCCCAGGCTGCAGGCTAGCCTGTTCTATT	@B06:@946?B<AC997=415?9=>81>:;7@5?H;/2AA8:99ICFDEFIF<>>>=23;D?>DF?EBDICBBCDGICFIFBBCDE?ACCBDAC?DFFFEEDEGEDDDDEC@DDDDCAADFEEFGEDFCDFFBCFBDCDABA=A?BCDBE	NM:i:2	AS:i:140	XS:i:19
host|32|0|1-150=chr7:21795364-21795514	83	chr7	23018560	0	150M	=	23018287	-423	ATAAATTTCATCCTTGAAAATACTTAATCAAAACAATGCATCCTCATTTGTTCAGGTAGAGAAAGATAGAGAGGGATTCTGTTCTTGTCCGAAGTTTGAAGTATCAACTAAGACGGAGCGAGAGTATCAGTTTGATCAGGACCATTTAAC	21,1)7FA5DCA=721(727>793?A?:AIIG9@<:CDA>?B?;II;8ADF<H:>?D=6=C?HABCEBC8<CDGDGFEHD@E>>AA@EDDBD>DDEEFEFDCDDEEEHFGGIEEFHHEFGEFCCDDDDBFCBDEIGHCC>@B;<D@@>9?	NM:i:1	AS:i:145	XS:i:145
host|32|0|1-150=chr7:21795091-21795241	163	chr7	23018287	0	150M	=	23018560	423	CATAGCCCAGGGTGAAGTCTTTACCAAGGTCTTTCAGGAGAAGATGTCCAGTGTGGATCCTGGATTCCTCTCTAGTCGTCTTAAGGCTTCTGAGCATGACCTGTTTCTGCCTCTTTTCCTATTTATTCTCGATATGCTCAGTAGATTGTA	?<==9=BBC@B@B@=BDDDDFFFGHDD?AACDGFEDDAFCFEFFEEFBCGFDGGFEEHDDADD?DEGFDGEDADA<A>BB?IFE@HFC@?HCCID5F<GCDE>@4E??>>?A88?=7>:9;ICA><@62D@=1+<68025=5/.14;C::	NM:i:2	AS:i:140	XS:i:140
host|33|0|1-150=chr7:18657610-18657760	83	chr7	18657611	60	150M	=	18657378	-383	GTTAGACCTAACTGTCCACTTGTTTTCAATTCTTGGACAAGATGTCCCCTTAAGCAGCTGATGACCAAGATTAACATCAGCTTCTCCCTGATTCACAGACACCTGGGAAGGTTCCAGGGTTGGAACACTGTATACGTGCCTGGAGACAGA	D=71-"-4,4>A=9H?>:D83<;<>><6.6<9::<:;;A@7><<<FHB=A@;7/7<?@>GIIG>B=A;:==BBDCE?ECBAABA@=CAACDEFIEGEFGECDCCDCBDABDDCB@BEFCBFEFDDDFEDBDGEEDDCBBABA?B<>B@;A	NM:i:0	AS:i:150	XS:i:0
host|33|0|1-150=chr7:18657377-18657527	163	chr7	18657378	60	150M	=	18657611	383	AGAGGGGTGTCCATAAACATAGGAAACAGATCAGGGTCTAGGGAGAGAGATCCAGGTTGGTCGGGATAGAAATGGAAAACAGTCTCCTAGGCAAGGTTATAGCTCAGAGAAACAATTGTCTGAGTGTTGAGTGACAACCCCACACAAGAA	:>=CD;>?<:>CDEBCDFEEEFHDDFFCBCEGGFFGFEECGHFHGECDCFEEEEEFB>>A=@?A=><?:@A;;7DI?D=>=CGDCIFIFIIDIHIB5//2<;??AF=>EE=>?@IE:<<=A:A919=A/5537,=-/BA37@DEHI6777	NM:i:1	AS:i:145	XS:i:21
host|34|0|1-150=chr8:103224897-103225047	99	chr8	103224898	60	150M	=	103225062	314	TCCAACTTTCACTTATGGAATACACAATTGTCATTATACAAATATTATTTAATTACATAATGGTTCCACTATTAAGAAATCTGTATTTAACTGATATTCACACCATATTCATAAAACACTTTATCCTAGGGACTATTTGAGAATTCTGTA	;@=A?<C<>BCCED=?ABCDECFEEEDBDFFGFEFEEFDCCCDDDHFACACDBBCBDBCDAFFGE@@>:<;;>@GFEGAA=;BAAECEBGC?=<B@C=<>>9DHCI?HIEA7<E>=>69:;3/>?1+:@05D98?:6:<;<8<92752AI	NM:i:0	AS:i:150	XS:i:0
host|34|0|1-150=chr8:103225061-103225211	147	chr8	103225062	60	150M	=	103224898	-314	GACAGGAGTACTTTTACTGATTGTATTTGGATAAGCCATGTTGTCTTTATAATAAGGTGTGTTGATATTAAAAAATGATTAAGCATTTCCAGGGAAGAGCTGCTTCATATTCACCTACCTTTCATCTCCGTACTATGAATTATATATCGT	E@?C=6881<14?<>B<67DC2*8<CADIFEIIC6>?@6DCEID?9;FC?DHI>>?DBD=;?AABGAIBAAA=?<G=A?DHFBA?FEFIHC@EDEEEEDABDBDDDDDHFB?DCBDCBFGIEFDCB@?BDDCDFCACDEAACFAAB>=<>	NM:i:1	AS:i:145	XS:i:20
host|35|0|1-150=chr3:117117199-117117349	99	chr3	117117200	60	150M	=	117117393	343	GGCTTTTGCTTCAGTTCTTCTCATTAAAGTGAGTGGGGCAATGACATGACAGAATTTTAGACAAAATTTCCATGCTACGTAGTAATTTTGTGGCAGTAGTAGATCACTGTGCTTAGAGATGTTGGAACCATCACCAGGAGTGACCAAATC	@:@AABB?@D?GFEEGFEBBFHIFFEFECHGEDCDFEDECFGFEDGFGFDFGGGCBGGAEFFBDFDCCGIDA??BBCD?:@DBA@AB=C@G>CA3687@?G>5:<:C<<EA;>8;7@BA<;B@::7=?IIBDCCEIBAG:;08/604733	NM:i:0	AS:i:150	XS:i:0
host|35|0|1-150=chr3:117117392-117117542	147	chr3	117117393	60	150M	=	117117200	-343	AGAAAGGAACAAAACAAATTCCAGAATGGACAGCGAGGTCCATCATCAACCCTAGAGTAAAGACTATTTCTGTTACTTTGTTTGAGTTCTTAGGGTAAAAATCTACTTTTTGATGAACTGTTTTCTACTCCTAAGAATATTCCAAATGAA	:<F=IHA;<C6B=?<1.1CEE>9:84;A<7:14FI@9DII>?<DEAFDBF>>A?F8<<BA?>9:<B=?BFEBFBC>;DBCG?D?=>FIIHH@BBCACDEEDDB@BDEEFFGEGGCBAEFHEEDDDEDDDCFCB@??C?<=B?@?>==;;=	NM:i:1	AS:i:145	XS:i:19
host|36|0|1-150=chr7:104248694-104248844	83	chr7	104248695	42	150M	=	104248537	-308	CCAGAAAGCTCTCACGAGGCTGAGGAAGGAGCAGGAGAAGGTGGAGACATTGGAAGCTGACATCAAAGAAGACAGACTTTCCTGGAAGGCAAGAATGGCTTCCTGAGTGATGCTGAGACCTATTTTAGGATGACCTTCTTTGATCCTCAT	4''307;>6;:6F.599IDEIF?46F:>96;9@?:HA@6344=5BID9:9@BH>B?>8FH?HFEB:85?GGAHEBBBHI@=9@AEEDBADEADDEFGDBDDDCBDACABDFCGAB@CDFHEEEBB@CBBBCADB@=AGD@B?@@@@?;;<	NM:i:0	AS:i:150	XS:i:145
host|36|0|1-150=chr7:104248536-104248686	163	chr7	104248537	60	150M	=	104248695	308	TCCACATCCTTAGACTTTGTTCTATGTTGTAAACACTACGCATCAACCTCACAGCTTTTATGAACTGGTCTCTTTCTGGGCACAAAATGTTTTCTTTGTTAGTTCACTGTCTAGGTGGAATGCATGACCGGTATGATTCCTTTCACACAG	C><=<B>C@@HE=ABEFGIGHFCFFGFCDDDCDBCDDFHGGEEBCD@CDEIHGBCBEEIGHGCIEDCCBGBABAAAAAGDD>AEI>CCDB>AB;9?BIAIE;>@=C>D>>?>=AB@;C@CIGB7:CC8?96:,353/$/98CBD?@G=>8	NM:i:2	AS:i:140	XS:i:98
host|37|0|1-150=chr14:83575308-83575458	83	chr14	83575309	60	150M	=	83575076	-383	TTGAACCCAAAGCTTTGCTTATGGTTAGCAATCAGTCCATTCCCAGACTATGCCTCCTCGGGTTCAAGGAAAATTTTCCTATAAATCAAATGAGTGTATATTTATAGTCAACATCAAAAGAAAATACCTTGAAACATGTTTACTAAGATA	BAIB;9=IB:5?BICB3,88849CA3,7C995=C=>45;IB;:?7:>A:3.=E=:>D?<CF@<I?:CEDGI@?:?BBA>@=;?A?B@DEFCCHHDEDCDDDDDEEDCDCFFFEECAADCA?BDGEFFGDCDDGIIIDDBFEIHDF?>=<:	NM:i:0	AS:i:150	XS:i:0
host|37|0|1-150=chr14:83575075-83575225	163	chr14	83575076	60	150M	=	83575309	383	GTTTATCAACAATGGTATTGCTACGTACATTATTTATATCCACCCGTATCTTTGAAAGAAGTGAAGCAGCCTTAACCTTTTTAGGTCTCCCTCTTTTTTGGCTGTACAAGGCCATCATTAACTTGCTTTGTGTCTAGTGGATGATGACTG	EBA<;<>>B>?>DFHEEEEBDFGDCCDEHEEEFHEDCDDDDBCBDEEBCCDDDBDBFDHIHD>?FIIDCC?B@HEHC=>A<?:>GEECD@>CA83GDAIICI??CAH===EBD;GDDGI@.;:9;?=>@<?=?<BGB>:=99BED?<50.	NM:i:0	AS:i:150	XS:i:0
host|38|0|1-150=chr4:35062389-35062539	83	chr4	35062390	60	150M	=	35062156	-384	CATGAGTGCATTCATACTTTAGAGAGATATGTGGAAAAGTGGATCTGAGCTTCCCCTTACTCAATCCTTATCAGTCTCAGAAGGTAGGAGGAGACAAATACTCCTTAGATCCCATTTGTTTTAGCCGCTGTTGTTTTGTTGTTGTTGTTG	'79:,?AIDA?9>@;>EA<<<167>AA?E;739<5:<C>FIIC;BCHC>@:@HIG?7=><:@?;>;A=CDG6?=>A@DFEABB?=BA?AEGHDAADDEFEEEDFGHIEGIEFGCBDFEDFHEEEFDFICADFGDDB@A?<A>??><<;>8	NM:i:3	AS:i:138	XS:i:24
host|38|0|1-150=chr4:35062155-35062305	163	chr4	35062156	60	150M	=	35062390	384	GAACAAAGCCTCTGCAAGATGCCCTACACACCTTGTATCCCTGGATCTAATTTCTTGGGAATGGAAATGCTTACGTTTTCCTGTCCTCAGCTTTGGAGGTCTGAGCAGAGAAAGCAGAAGCTACTCCACAGTCACATGACTCCCTCCGCC	>?>??BA==>?@=@GFDEDCEEEIHFGHECFFEHDDDCEEFBDBDFEEDCBCDDECD@C?A@BAECFH@E@AGDB<>>BHHICAC<8@=>G;=9<7FGIFIH<2@=8>FDHC=IEFA1A<<<=A=638857246:?A5?A@B@4=BCB<A	NM:i:1	AS:i:146	XS:i:0
host|39|0|1-150=chr4:95861168-95861318	99	chr4	95861169	60	150M	=	95861518	499	CAGTTTTCTAGCACAGTGACATTACTGGCTGGGGAGTGTAGGGCCTAAGAACTTAATCTCTTGACCAGTCTGGCACGAGAGGAGTGCTAACGTGAAGCTCCGCATCAATCAAGAAGGGGAGCAGATGCTGAAGTGTATCAGATGTCAGTA	B@>@;@AEGA@>@BDAAEFFCGFC@BBCDDDFIGFEDDEFEFEEEADAACCEEFEFFACCHEIGGGHFFCE@@A8<99;>>=IGFADDBB;ACE=HIIC?GACG>883;0>CE?ABD<@ID@:6:C63=;;>IGD69BGIF===:<0?6<	NM:i:3	AS:i:135	XS:i:0
host|39|0|1-150=chr4:95861517-95861667	147	chr4	95861518	60	150M	=	95861169	-499	ATTTTAAAGAATACATTTAGTGCTCAGAGTATTAGTGGTTGCCAGCCTGGAATTACAGTTTATCAAGTGTCTTTCCAGTGACGAATTTTTTATTTTTCTCATCTAGTAACCCCTCTTTGTTTTTACTTTATACAGTATAATTCACCCAAT	/.@6==AH>9B;8EA?39698:>;2)5536:6<;9IHD>>?79@FE;I;>@B@AB=::49@CC>CICE>BDEBC?<C?>DFIIIDCDEFEGFGDBEFDFGFCFEEECAD@A@BCCECEEEEDEEFEGFFGDDCB?@<@=@>@HIAA<??@	NM:i:1	AS:i:145	XS:i:0
True_Junction|00|1|1-150=chr18:39199613-39199764	97	chr18	39199615	60	150M	AAV_RSV_GFP	511	0	TTTTGACTTCTTGCACTTAAAACCTACCTTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCAGGGATTTGATGTTTGTTCAGGGTCAGATTCTTGCAAAGTA	A?A@<?@CBAB?AHHIEEEEBCCCFEED@CDFFIFBCDDDGFEHFHDDEEEDEECEBDGDCGEECCAB@=<D<=9=A=B=?ICI;A=HEDG966;9DB>I=>@<@DA@>DCC@EI:8??FIIII@BAA?>6:;=FIBIA@=74;;/+5&0	NM:i:1	AS:i:145	XS:i:0
True_Junction|00|1|1-150=AAV_RSV_GFP:509-660	145	AAV_RSV_GFP	511	0	150M	chr18	39199615	0	GTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATTCTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGAT	+51?HI>>DIBFE<34759697:5BF3)9>7;;:D>CIE@A?A>??CB=<>IGA<9FDIAG9@;B<98<@>BB?ABIIIA?I@CCCC?>CB@<ABCDDBA@CEFHDFEEDCBEHEFFDCBEGIGECDCBDFEEDDFHF?@>?<@@@A>EE	NM:i:1	AS:i:145	XS:i:145
True_Junction|01|1|1-150=chr18:39199566-39199717	97	chr18	39199568	60	150M	AAV_RSV_GFP	523	0	TCCTTATCAACTCTAACACAGGGATTAGGGGACACATCATTTTTTTTTTTTGACTTCTTGCACTTAAAACCTACCTTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGAC	=@?@GHC?>@=@ABBBCEDCB@FEBCDEIGGGFEEIHFEEFFFEEEEEFDCEEFHFIFFEEIGEDGCHHHCGIED?A@IDIF<DDED<7DH<>BC@<BECIB<>739?B@G=;@B;8<9;:5BF;<=@11>?B<E?DE@*.-)9:+8B;9	NM:i:0	AS:i:150	XS:i:0
True_Junction|01|1|1-150=AAV_RSV_GFP:521-672	145	AAV_RSV_GFP	523	0	150M	chr18	39199568	0	TTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTA	08865;98AEB>D?B<:30788,6959;)1:;)3>95HI@=CHC=>>I?@>AEAB=?<B@FCD<?<8=ABBDBA?<9AB>?GFEBCBABFFBEEDEDDB@DDDDDECHEEFHGFGFCBDCCDEDDDDBB?BB?@BBAB?@ABD<@?@>?<	NM:i:0	AS:i:150	XS:i:150
True_Junction|02|1|1-112=AAV_RSV_GFP:473-585|113-150=chr18:39199727-39199766	81	AAV_RSV_GFP	474	0	38S112M	chr18	39199518	0	TTTGATGTTTGTTCAGGGTCAGATTCTTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATGGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACG	D89GID?9>7;4:2:62(=?62A:;>8FGB;4:B>:6?9=H?>>=9<<DI9B@C7A>??A<EFH@E>8FD9;==FDFH;BDBCDFHFHIGIFGCDEEDCEDEFGCDCDCEFGEBBBDCEEEBDEEEHDDCEEFFCAFAA?>>?>>>>?86	NM:i:1	AS:i:107	XS:i:107	SA:Z:chr18,39199729,-,38M112S,0,0;
True_Junction|02|1|1-112=AAV_RSV_GFP:473-585|113-150=chr18:39199727-39199766	2129	chr18	39199729	0	38M112H	=	39199518	-249	TTTGATGTTTGTTCAGGGTCAGATTCTTGCAAAGTACT	D89GID?9>7;4:2:62(=?62A:;>8FGB;4:B>:6?	NM:i:0	AS:i:38	XS:i:0	SA:Z:AAV_RSV_GFP,474,-,38S112M,0,1;
True_Junction|02|1|1-150=chr18:39199516-39199667	161	chr18	39199518	60	150M	AAV_RSV_GFP	474	0	CCCAGGAAATAGTAGACCTGGCTTACTTTTTAGGTCTAGATAAAACTCTGTCCTTATCAACTCTAACACAGGGATTAGGGGACACATCATTTTTTTTTTTTGACTTCTTGCACTTAAAACCTACCTTCCTAATCAGTGCTCTTTAAGCAG	>>>?@AAA?@DCBAE>CEFFEEBDFHFAACEEEBCGGFEEEEHDEDCBCDBEEGGEEHFC>ABC@HHEEIHEE:@DC?ADHECEIIEFCD<@GED;C>G@?>?A2,4<37495B?>AG?>=<9<:244;9:?=7;;;:C973;8-:7DHC	NM:i:0	AS:i:150	XS:i:0
True_Junction|03|1|1-138=AAV_RSV_GFP:473-611|139-150=chr18:39199753-39199766	81	AAV_RSV_GFP	474	0	12S138M	chr18	39199643	0	TTGCAAAGTACTTTCGGATGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGG	5><BCG7:47>7A@B:?./*.45;F@?@?98@=84A?@C8>6@<;1254;;49C=GDIII@IIHHBABADB;;@?A?ABFIDFGC@AGBAA>CDDBBCEEEFHEDDICDDBED@ACHEEEECBADCDDDEFDEEB@CBAC=B?>;=;>A@	NM:i:1	AS:i:133	XS:i:133
True_Junction|03|1|1-26=AAV_RSV_GFP:473-499|27-150=chr18:39199641-39199766	161	chr18	39199643	60	124M26S	AAV_RSV_GFP	474	0	TTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCTGGGATTTGATGTTTGTTCAGGGTCAGATGCTTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCC	>B@D@AA?>AA@CGED@CEEDIGIFFEEDECDDDBFEEFEEEEFEGCCDECGACEDDDB?A?BCFIFC>BIFE@BGDCC;@DCD?CBADEHA?:4=@?I>DI???D@=IGFI8@<B?559F>;;5>=E:5@C:><>:>A=?IA<A==3.8	NM:i:1	AS:i:119	XS:i:22
True_Junction|04|1|1-150=AAV_RSV_GFP:513-664	81	AAV_RSV_GFP	515	0	150M	chr18	39199636	0	TTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTG	357,8B8?GIDC><@<9:87E@37@7=<I?<;9=<:ADIHD;F?FF:@ACH=9:8DC;@F?=;B>>;:>I?<>9<>:EGD@IDECCB@B@BA@BFEEDCEEFEFHECFGFCBA@?@ABDDDCBBCGHACEEDEEFAC?<=>A@?@AAB@=	NM:i:0	AS:i:150	XS:i:150
True_Junction|04|1|1-19=AAV_RSV_GFP:473-492|20-150=chr18:39199634-39199766	161	chr18	39199636	60	131M19S	AAV_RSV_GFP	515	0	ACCTACCTTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCTGGGATTTGATGTTTGTTCAGGGTCAGATTCTTGCAAAGTACTTTCGGTTGTACGCGGTTAG	?CBB?>?A?AD>B=AFIGFDDDDEEBCDBEDBCDBCDCDCBBCBCDFECDCCDEDECCDFED?BA=ABBCC:DDC?8@A>EEGIDEDDI=C=E>18IDEHGA53><7C@E>978AIDE@EGI@?<40B<@A;A>F9I?@A;?7797:':?	NM:i:0	AS:i:131	XS:i:0
True_Junction|05|1|1-128=AAV_RSV_GFP:473-601|129-150=chr18:39199743-39199766	81	AAV_RSV_GFP	474	0	22S128M	chr18	39199591	0	GGTCAGATTCTTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATG	@=3<>6C356@:>E:97?.>?GGC=;CIAFF=?;>95<=9DI?A<940146>A>?;7607;;:AC?>8:?<==?=8?@@=>9@@@DEDDFA?CDFHCCDFDEFGC@EEEEFFGFC>?@DACACDEEFEEEBDDGGDDDFBBDBC?BA>>@	NM:i:0	AS:i:128	XS:i:128
True_Junction|05|1|1-150=chr18:39199589-39199740	161	chr18	39199591	60	150M	AAV_RSV_GFP	474	0	ATTAGGGGACACATCATTTTTTTTTTTTGACTTCTTGCACTTAAAACCTACCTTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCTGGGATTTGATGTTTGT	@=BDBAAACCAAB>9BA@BCHFDEEHGC@BBDEFCFECBCCDABCEGFIEFGFHHGDCC?ACBCECGEC>E><=:==BIHIIA;?>EFDC6?38IA??<4;4?926<>E;8@>BHBB427>80-30E@I@?AD<7=G>617??IA963<@	NM:i:0	AS:i:150	XS:i:0
True_Junction|06|1|1-3=AAV_RSV_GFP:473-476|4-150=chr18:39199618-39199766	97	chr18	39199620	60	150M	AAV_RSV_GFP	500	0	ACTTCTTGCACTTAAAACCTACCTTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCTGGGATTTGATGTTTGTTCAGGGTCAGATTCTTGCAAAGTACTTTC	<?=@9<@CAAADD?AACDDCCDDDBCCEEGEEEEFDDDFCDDDDDDCACFEDIFB@CDI@>A>D<AB=@ABBA=BB<>==9=8?@A@:>??ED<@?G<BIB(7>??;/6<DI=<==58BF>DCIH8=?CI?2@9=:I;<?9<7;CE?>C4	NM:i:1	AS:i:147	XS:i:0
True_Junction|06|1|1-150=AAV_RSV_GFP:498-649	145	AAV_RSV_GFP	500	0	150M	chr18	39199620	0	CTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGTGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTTCATGCCGATTGGTGGAAGTAA	7GC<;97;:;AIC:3>?;??.96:E;;5BF>@<9I=<;=G6B@ACI??;=;B9=?G@DIEABF?B@A=>B=7>:A@?@=GFADF@@@DDEIDDEGDFBDCCEFDDCACDCDDDDFHCBDEEEFEEBFEFHDCBDADFCDCC=:@@?@>B>	NM:i:2	AS:i:140	XS:i:140
True_Junction|07|1|1-150=chr18:39199556-39199707	99	chr18	39199558	60	150M	=	39199669	209	TAAAACTCTGTCCTTATCAACTCTAACACAGGGATTAGGGGACACATCATTTTTTTTTTTTGACTTCTTGCACTTAAAACCTACCTTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACT	CEEB:;<<>ADEHDGCEEGFGFGEDCCBCCCEFHGFGIHGDDDEGIFEEEFFDDDDCACCB?=>=CEGC>;AGHB?@>E>@=CB<7:BDGDDB?DIGIIC<D>A93BG8;=@I78;A>8:70;6:5:HCI>?<E4-*23B>34:;:<?06	NM:i:0	AS:i:150	XS:i:0
True_Junction|07|1|1-52=AAV_RSV_GFP:473-525|53-150=chr18:39199667-39199766	147	chr18	39199669	60	98M52S	=	39199558	-209	CTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCTGGGATTTGATGTTTGTTCAGGGTCAGATTCTTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTG	&"01423459G>;88@2-73;<?<AF;99:AGII>75.?<7;9481>A85<:?B:>?F<8ABCEIIHEAA?=:<DIEBA?A?D@D?B@A@CCCEGEEEFDEEECEEFGDCACFFCACBDHGABDDDCBCDDCFEFADDBA@CE==?:<<<	NM:i:0	AS:i:98	XS:i:0	SA:Z:AAV_RSV_GFP,474,-,98S52M,0,0;
True_Junction|07|1|1-52=AAV_RSV_GFP:473-525|53-150=chr18:39199667-39199766	2195	AAV_RSV_GFP	474	0	98H52M	chr18	39199558	0	TTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTG	FDEEECEEFGDCACFFCACBDHGABDDDCBCDDCFEFADDBA@CE==?:<<<	NM:i:0	AS:i:52	XS:i:52	SA:Z:chr18,39199669,-,98M52S,60,0;
True_Junction|08|1|1-97=AAV_RSV_GFP:473-570|98-150=chr18:39199712-39199766	81	AAV_RSV_GFP	474	0	53S97M	chr18	39199622	0	GGACAAGAGCTGGGATTTGATGTTTGTTCGGGGTCAGATTCGTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCT	261)8>6?49DEH4::>9:745)&51!7:;<AI?:9B=?B;<>4@DGIF@>=8A;;:<8?8>>;?B>::<GDFC?CDFDAD@DD?AGFHEC?CHHHFABDFDCFGBBGGGDCEEHGE@DDC?@B@FGDCDEFFCFEDBB??<ABAD@ACB	NM:i:0	AS:i:97	XS:i:97	SA:Z:chr18,39199714,-,53M97S,0,2;
True_Junction|08|1|1-97=AAV_RSV_GFP:473-570|98-150=chr18:39199712-39199766	2129	chr18	39199714	0	53M97H	=	39199622	-145	GGACAAGAGCTGGGATTTGATGTTTGTTCGGGGTCAGATTCGTGCAAAGTACT	261)8>6?49DEH4::>9:745)&51!7:;<AI?:9B=?B;<>4@DGIF@>=8	NM:i:2	AS:i:43	XS:i:20	SA:Z:AAV_RSV_GFP,474,-,53S97M,0,0;
True_Junction|08|1|1-5=AAV_RSV_GFP:473-478|6-150=chr18:39199620-39199766	161	chr18	39199622	60	145M5S	AAV_RSV_GFP	474	0	TTCTTGCACTTAAAACCTACCTTCCTAATCAGTGCTCTTTAAGCAACCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCTGGGATTTGAGGTTTGTTCAGGGTCAGATTCTTGCAAAGTACTTTCGG	F@?C@@@@DDABB@DEEEDDFFEFGEEFEDDFEEDBECCCDBCDEBCBCDFFFFEDD@CGEDDFBBAAAFIFFD@@@IHGCDEGCA?I@@C9<;A@@@@9I>@EFE95D;9;:<<>;4@4AHBE;?B;H69:BGI=:DBIIBIEIGCI89	NM:i:2	AS:i:135	XS:i:19
True_Junction|09|1|1-150=AAV_RSV_GFP:540-691	81	AAV_RSV_GFP	542	0	150M	chr18	39199575	0	TGTAGTCTTATGCAACACTCTTGTAGTCTTGCAACATTGTAACGATGAGTTAGCAACATGCCTTACCAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTC	@<6989?=<5)627>>04.:=DC70/0.97@GDAD;AA><<>AHI3==BF@E?3;9EB9?GBGCIFGEG?9A@?EEHBIFD<?DBA?<@EECBDDEEFFEDEEEDCGHAAAFFFHGEEDFDHF@BBDCDEEDDDDCEGGFCE@?AABDBF	NM:i:3	AS:i:135	XS:i:135
True_Junction|09|1|1-150=chr18:39199573-39199724	161	chr18	39199575	60	150M	AAV_RSV_GFP	542	0	CAACTCTAACACAGGGATTAGGGGACACATCATTTTTTTTTTTTGACTTCTTGCACTTAAAACCTACCTTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCT	ABAA@@=:>@@@ABC?BCDCCEECDDCFFCCCBEEGBDCCACDGHHFGFGFDECAC@DC==E@EGEFDBIEBBBBDFHIBA>58A?IHC;;>9;>9C;8A95???>><@F</;?DE28BGFI:D@?@78A1897146058648682,387	NM:i:0	AS:i:150	XS:i:0
False_Junction|00|1|1-150=chr6:55819046-55819197	97	chr6	55819048	60	150M	AAV_RSV_GFP	1188	0	CCTTTTTCTGTTCCTGCTCTGCTGCAATAACAGCTCGCTCATCCCCAAGGGAAGCAGGACTCACCGCTGATGTGAGCATAAACAGTCTAGCTGCATCCTGTAGCTATCAGGAAGTCCTTGCTACTCAGGAGGGCAAGTGGACCCTGCAGT	9>>BBBAA>ABCECGHHIIIIGFFFFFDFEFHBACCHEIDACEEGDDDCDDDEHGDC@@=DAABA<<>AEGAIA>@A<EFEID@BDBGDG=BIHAIF<?CDGHDII49A:@B:EIHFA69=;G178<3?A=<E<:?=A59@@>@@94967	NM:i:0	AS:i:150	XS:i:0
False_Junction|00|1|1-125=AAV_RSV_GFP:1188-1313|126-150=chr6:55819199-55819225	145	AAV_RSV_GFP	1188	0	24S126M	chr6	55819048	0	TCAAGCAGCTGAGTGGACATTGTAGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCAC	83/-.1329;;9?12:7:73;91G:D<5.><F;69=<@>=ED6126@?<9D@<@A88;?9:<AD>F??=A@A@;DFE??<?==?@AFBABA?>ADFDEECEDEEFEFEEDFDGFEFGEEEDDFEFEFDCDDDCDFEF@A@AA@B=;>?<:	NM:i:0	AS:i:126	XS:i:126
False_Junction|01|1|1-150=chr14:17049267-17049418	97	chr14	17049269	60	150M	AAV_RSV_GFP	170	0	ATGCAGAAGAACTTCCCTAAAGCTTAACCAATATGAACTGCCATTGATCCAAGGAGAGACCCAGATTTCAGTTGACACAAAACAGTCATAGGGCCAAGGGACCCATTCTACACCACACTACTCTATGGAACAAGATACAATTTGGCTTTA	@>9@=GE@BADFDGGFFEECEFDBCGEEEDCGEEEEDDDDGDECDGCECCEEBADGFAD??BAE?A?@CCDCEAA?A?@?ECIH??F?@=;69D>7@@B>D>@28=>:@C?=AA39;7=;<2E=C<>;;5A>3A9<=I=559=?ABCIBE	NM:i:1	AS:i:145	XS:i:0
False_Junction|01|1|1-90=AAV_RSV_GFP:169-259|91-150=chr14:17049435-17049496	145	AAV_RSV_GFP	170	0	60S90M	chr14	17049269	0	GCAAATCTTTTTTTTTAAGTAGGAACAGCATCAAAAGCAAATTTTAAGAAAAGAAGCCTGGCCATGCTCTAGGAAGATCTCGACGCGTCATGTTTGACAGCTTATCATCGCAGATCCGTATGGTGCACTCTCAGTACAATCTGCTCTGAT	.;9F7475B>I80;>27EEHE?784@?A?B@659IFI?505;5=7959>B;C39IDF>AH;BAAE@@ABCBCBC>;@?@CB@>:;:=DEFEDBFDFHEFIFEDDGEDC@>@@EEEECCEEDDDDDDDFBEDFDADEGGDE>DEDH@>?=A	NM:i:0	AS:i:90	XS:i:90	SA:Z:chr14,17049437,-,60M90S,0,0;
False_Junction|01|1|1-90=AAV_RSV_GFP:169-259|91-150=chr14:17049435-17049496	2193	chr14	17049437	0	60M90H	=	17049269	-228	GCAAATCTTTTTTTTTAAGTAGGAACAGCATCAAAAGCAAATTTTAAGAAAAGAAGCCTG	.;9F7475B>I80;>27EEHE?784@?A?B@659IFI?505;5=7959>B;C39IDF>AH	NM:i:0	AS:i:60	XS:i:0	SA:Z:AAV_RSV_GFP,170,-,60S90M,0,0;
False_Junction|02|1|1-150=chr19:28045506-28045657	65	chr19	28045508	60	150M	AAV_RSV_GFP	1225	0	TCAGCATCGATGTCTTCATCAGTCACGCCCCAGGAAACCTCAAAGTGTTAATGTGTTATTGGTAGAGGTGTTAACAAATGCTATCCCCACCAATAATGGGCAGTCGAAAGCACAGCCCCTCTACTGTCTGCCCTGTCACTTACTTTTTGG	@@@??AAA@ACCCDAACFFFEGFFEEEDGGFDDBCBACEEEDBCDEEBAEEEEDDDFCCCDDCACCDDA?BAD@AAACBDBBD@9F<:=7???6A>CECIID>@B@C?DGB9>CACA<:;928IHIE9=@-1:5::9B@<6;B5@E?<:C	NM:i:1	AS:i:145	XS:i:19
False_Junction|02|1|1-107=AAV_RSV_GFP:1224-1331|108-150=chr19:28045988-28046032	129	AAV_RSV_GFP	1225	0	107M43S	chr19	28045508	0	GTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGATGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGATGGCTTATGTTGCCTGTCATAATTTTTTGAAGCCCAGCATTTT	?<AAABF@CB>>A@@@AACBCDCEFGFEDEDDDAFAEECFAEDDCDDDD>?ACEFFGEBB99>A@BBCBFGFF?BB=C>GA99<ABA>GAA=><B;B<>?559:<=AIA;4;;47=A<<7B45<<A/293<@II@:>@G79C:D=B;?FG	NM:i:1	AS:i:102	XS:i:102	SA:Z:chr19,28045990,-,43M107S,0,0;
False_Junction|02|1|1-107=AAV_RSV_GFP:1224-1331|108-150=chr19:28045988-28046032	2193	chr19	28045990	0	43M107H	=	28045508	-525	AAAATGCTGGGCTTCAAAAAATTATGACAGGCAACATAAGCCA	GF?;B=D:C97G@>:@II@<392/A<<54B7<<A=74;;4;AI	NM:i:0	AS:i:43	XS:i:0	SA:Z:AAV_RSV_GFP,1225,+,107M43S,0,1;
False_Junction|03|1|1-150=AAV_RSV_GFP:1260-1411	113	AAV_RSV_GFP	1262	0	150M	chr1	97749462	0	AGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACT	74/+/>9;9<18<<@6=7>@:.:=DE@CC:D32<::9?C:CIDC0@CHECG?76:5<7;=@=4@FDE;:59=A9?BFBAEA@CEA?BCAEIDDEFFGECCAFFCFFFEFFEFHFGGHFFCFEFEGCCEEEFEFDBGEHDA@CAB?=@@=A	NM:i:0	AS:i:150	XS:i:150
False_Junction|03|1|1-34=AAV_RSV_GFP:1160-1194|35-150=chr1:97749461-97749578	177	chr1	97749462	60	34S116M	AAV_RSV_GFP	1262	0	CCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGCCCTCAAGTGATCAAAAAGTTCCCATGGCTAACACATGCTCAAATGCACGCAGGAGGAAGGTTCAGTAGTAAATGCTTACCCAAGAATTTTTGCACAGTCATCATAGTACTTCATG	6D:0$541997>:;>B@A;?6,BIIICE<=?>;=>C9;IIFIIIDII=@=7;=>=@A;B=HI>>CBCD@>HCGAAAD;ABCACC@DAEACCB?DBCBCBDB@DEAFDD@@CBBDDEEEFFCEFHDCDDCACIGIFEFFIHE><<79>>A?	NM:i:2	AS:i:106	XS:i:0	SA:Z:AAV_RSV_GFP,1161,+,116S34M,0,0;
False_Junction|03|1|1-34=AAV_RSV_GFP:1160-1194|35-150=chr1:97749461-97749578	2209	AAV_RSV_GFP	1161	0	116H34M	AAV_RSV_GFP	1262	0	CATCTGCACCACCGGCAAGCTGCCCGTGCCCTGG	=;>?=<ECIIIB,6?;A@B>;:>799145$0:D6	NM:i:0	AS:i:34	XS:i:34	SA:Z:chr1,97749462,-,34S116M,60,2;
False_Junction|04|1|1-150=chr13:43098212-43098363	81	chr13	43098213	60	150M	AAV_RSV_GFP	1059	0	AAGACTTCTCCATGGACAGAGTAAAATGCCAGCATATCTGGTTGCCTAATGTAACATTGTAGAAAATGACAGACAGAATAAAAGTGAGAAGTGAGGAGAGGGAGAGATGAGAGATGCAGAAAGCAGTCCTGCCCACCCTGGTATTGACGA	50/D:97BAG85B<674:898%5,,8II?9608=:4159@B?BA;=AC99E>=A:A@G<@D9BC:><=8>;?@A@GBB@B@CBABFDA>>EGEDFHECBA@@CADCDCCBEECFHEFEEEEDDDCBDCBEDCCBBEB><<BCDCF@B@;?	NM:i:0	AS:i:150	XS:i:0
False_Junction|04|1|1-76=AAV_RSV_GFP:1058-1134|77-150=chr13:43098149-43098224	161	AAV_RSV_GFP	1059	0	76M74S	chr13	43098213	0	GCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCGCCTCCCATTTCCCAGACTTTCCTATAGAACAAACAGAGCCTGTAGTATATGGAGGCCCATGCAAGACTTCTCC	HC@C@A?@@A?<BDACDDDDCDBBCDDDCCBBBCBCDCBDDADEFCIHECBBAACDCB@@C?AGHCEEDC>;BB@?@FEC;A=A<A>6AA=:=C??BC8DGBAFBH>8;8<58<8C@5<A7;>5D529=;DGIDCI<8:AFD;E;DC@42	NM:i:0	AS:i:76	XS:i:76	SA:Z:chr13,43098149,+,75S75M,0,0;
False_Junction|04|1|1-76=AAV_RSV_GFP:1058-1134|77-150=chr13:43098149-43098224	2209	chr13	43098149	0	75H75M	=	43098213	214	CGCCTCCCATTTCCCAGACTTTCCTATAGAACAAACAGAGCCTGTAGTATATGGAGGCCCATGCAAGACTTCTCC	?@FEC;A=A<A>6AA=:=C??BC8DGBAFBH>8;8<58<8C@5<A7;>5D529=;DGIDCI<8:AFD;E;DC@42	NM:i:0	AS:i:75	XS:i:0	SA:Z:AAV_RSV_GFP,1059,+,76M74S,0,0;
False_Junction|05|1|1-4=AAV_RSV_GFP:1708-1712|5-150=chr18:30329153-30329300	81	chr18	30329154	60	4S146M	AAV_RSV_GFP	1416	0	TGACATGGGGCTGGGCATCTGCTCCACTGTGAGTCCCAGGAGAAATGACACCATGAACATGTCCATTTTTTCGTGACTCGGACAGTCTTTAATACTGTGTGCGTCCCATCTCTTCCCCCTCCTAGGTACCATAGCTACCATAAGAGCAGA	203+9<286CA?72@>8890816?9;?59:52;9:<8HCCFAB5,58>:6>4>?F<IHEAB=><?>;8=9IC@BGHA?BCHA?DABB@CEEBCCEBBEDDDCBFFDEEHHFHHCCDDEGIEDEFGHIGIGEHFDDCB@FDAAABD@?@=?	NM:i:0	AS:i:146	XS:i:0
False_Junction|05|1|1-150=AAV_RSV_GFP:1415-1566	161	AAV_RSV_GFP	1416	0	150M	chr18	30329154	0	GGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGTTCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCA	FACDFIIEEFFGCDCFFFDCGFEEDHFEFFEGEEEGDEEBDDDDDEEFGHEFGFBCB?<A@@D?AABB8=>@@B??=?BA?<75C=?A38C9=99133435;<>@C>>=ID==<C@51/7=<8G9FGFI9<81//:959<=5768@<CBB	NM:i:1	AS:i:145	XS:i:145
False_Junction|06|1|1-46=AAV_RSV_GFP:730-776|47-150=chr2:59284184-59284289	113	chr2	59284185	60	46S104M	AAV_RSV_GFP	775	0	CAAATGGCGTTTATTGTATCGAGCTAGGCACTTAAATACAATATCTCCTGCATCATAAAATGGTTAATCTGCCTCTTTTGTTTAAAAAAAAAATGATTGAAAAATCATTTAGAATTTACTTTTAAAATTGAACAGCCCTACAAATTTCCC	6'6598C<24061+.2)656:9<=;?AE<9@ABF69>94B=<C4;81:;7?<;:ABA8<6:4@@=9@HDCFGD@EGBAGHI@ACC=@@AAB@BDEEEDEFGIIEDCDEFFFHFEHIEFEFEBCABCCCEEDDFGGHEFIB>FD:>?>AAF	NM:i:1	AS:i:99	XS:i:19	SA:Z:AAV_RSV_GFP,730,+,103S47M,0,0;
False_Junction|06|1|1-46=AAV_RSV_GFP:730-776|47-150=chr2:59284184-59284289	2145	AAV_RSV_GFP	730	0	103H47M	AAV_RSV_GFP	775	0	GAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTG	18;4C<=B49>96FBA@9<EA?;=<9:656)2.+16042<C8956'6	NM:i:0	AS:i:47	XS:i:47	SA:Z:chr2,59284185,-,46S104M,60,1;
False_Junction|06|1|1-150=AAV_RSV_GFP:773-924	177	AAV_RSV_GFP	775	0	150M	chr2	59284185	0	TGACCATTCACCACATTGGTGTACACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGAC	>;>99?859?=>537,!3-<=>HIIF788<?GB=?ABADG9=;8AA:=C>A?=A9;D87BDC@@=73>ADC??CA=A@AABIDE@FBA>FED@CEDBBFGFFFFEDA?>DDCECEEDECECEEDEBAAECAEEFHFA>BA>??=@<9656	NM:i:1	AS:i:145	XS:i:145
False_Junction|07|1|1-17=AAV_RSV_GFP:1233-1250|18-150=chr10:17253821-17253955	65	chr10	17253823	60	129M21S	AAV_RSV_GFP	1104	0	ACTTTCTGGTGGCTTTGAACACATTGGTTATGTTCCTAAGCTAGCATTTTCTTAACTGTCCCATAGAGTGACAATGTTCTCAAATTTCTTCTGATGATATTCTGTTAAGAGTCACACCACAATAGTAAAGATTTCGGGGTAGCGGCTGAA	<@=BCD@;CCBAFAFGFCBCEHGGFEHFBCACCDCEFEEEGEFEEGIEAAEDDDEEDDGD=CBBCCFBD@7>CGD@B@BBDAIIE;E@9BHA94;5:@B=AE=C>.<=?A9?BC9@CDBI9ABA:8:=?=CEI??@<7BF?.45<6639:	NM:i:0	AS:i:129	XS:i:0
False_Junction|07|1|1-147=AAV_RSV_GFP:1103-1250|148-150=chr10:17253951-17253955	129	AAV_RSV_GFP	1104	0	147M3S	chr10	17253823	0	CAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGAAAT	>=?<>B><ECCF?ABIGFGHFDDDFACDDEEEECCBCEHEEECDDHCDDAACFFEEDEFIIGDCCFECIFIDCGD?@?DIHF<=@EBBGII=>?C@@B<AA@>>C<69=IIAEGGHB=DA>I>?E>A8@5=BH80==?=1@<99474=83	NM:i:0	AS:i:147	XS:i:147
False_Junction|08|1|1-150=chr2:59284477-59284628	113	chr2	59284478	60	150M	AAV_RSV_GFP	730	0	TTTCTTGAAGGACCTTCCAGGTCATCGAGGAGTACACAGTCAGTTTAGACAGTAGGGCCCACGCTGTGTTTACAGTTTCTGGAGTAGTTTGCATAAGTGAAGCTTAATTTGTTACTGGCCATGGTTAATTGCTTCAGTCATTGAAAGTTC	*2852-0:.--4+69:/;:=46.2:616:AB;DAB?A;8D?;;<9:F=HGCAGDBE@I6?;;ADGIHAE=?=C@GFIDC@C@?<8?@ADEIGBACCDCAGEGFFGEFEBGGFFFFFICCBDDHGIEFDEGFGDCEDAACDDE@@BD?AC?	NM:i:0	AS:i:150	XS:i:0
False_Junction|08|1|1-117=AAV_RSV_GFP:730-847|118-150=chr2:59284184-59284218	177	AAV_RSV_GFP	730	0	32S118M	chr2	59284478	0	AAAAGAGGCAGATTAACCATTTTATGATGCAGGAGATATTGTATTTAACTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGC	EA@42??>3+<68CIFHG=:40:<A>B76>E<2A?=B?B8@AE=34:6A?<:@DAII>7CFIDFGACH?<CHA<=E?CCACAB>BFABDHDDCCAEEFGHIHHEFFDCFGHEECBBCDCFEEFEDECBDGDFFEEE@AACFBAC@B@@BF	NM:i:1	AS:i:113	XS:i:113	SA:Z:chr2,59284185,+,117S33M,0,0;
False_Junction|08|1|1-117=AAV_RSV_GFP:730-847|118-150=chr2:59284184-59284218	2209	chr2	59284185	0	117H33M	=	59284478	443	CCTGCATCATAAAATGGTTAATCTGCCTCTTTT	2<E>67B>A<:04:=GHFIC86<+3>??24@AE	NM:i:0	AS:i:33	XS:i:19	SA:Z:AAV_RSV_GFP,730,-,32S118M,0,1;
False_Junction|09|1|1-150=AAV_RSV_GFP:1744-1895	97	AAV_RSV_GFP	1745	0	150M	chr6	17936549	0	CGGCCGCAGTTACGCTAGGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTAT	D??A?B?@?@?ADG@FEFDEFHFHFEDDDDDACDACDEDDABCDFEFEECBCEFEFCDEA@A?A@>BEB?CCC?FF?BB@FCG>>:<CH>D<<>FB@67@46,5597=@>BD?>AIFIB9@CEB@79B@=4<6:>@9>=@=88842;I?3	NM:i:0	AS:i:150	XS:i:150
False_Junction|09|1|1-10=AAV_RSV_GFP:1886-1896|11-150=chr6:17936549-17936690	145	chr6	17936549	28	9S141M	AAV_RSV_GFP	1745	0	TGCTTTATTTCCCTGTGCACGGGCTCACAGTGAATATCCCTGTGCACGGGCTCACAGTGAATACCCCTGTGCATGGGCTCACAGTGAATGTCCTTGTGCATGGGCTCACAGTGAATACCCCTGTGCATGGGCTCACAGTGAATGTCCTTG	;9?:A=950<>BI=@7;27496=9=;E34=9>IGIED<9FEGCCFFD>D@ACH;B;>;:?@@?=FIABEHHB=B:B?CCDBBA@@FDDFCCCACB@CFFFGHGGEEDFEEFAEFGDEFGFDDDDDDCA?@HHDDA?A@D>@AABFCFB@B	NM:i:0	AS:i:141	XS:i:114

This also work. But I needed to modify run_command function since BWA always output a repport to the standard error output.

I need to build a more complete wrapper for bwa taking into account the possible errors and able to generate an index at instanciation.

Splitting of packages in separate folders

To isolate each class in a single file while keeping the organization as packages, I needed to create folders containing an init.py file. To import classes it is now necessary to indicate the name of the file AND the name of the class it self. See example bellow.



In [2]:

    
from FastqQualityControl.FastqFilter import FastqFilter
from FastqQualityControl.QualityFilter import QualityFilter
from FastqQualityControl.AdapterTrimmer import AdapterTrimmer
from FastqQualityControl.PairwiseAligner import PairwiseAligner

min_qual, min_size, read_len = 31, 100, 150

input_qual = "fastq-sanger"
R1_path = "../test/fastq/1kseq_R1.fastq.gz"
R2_path = "../test/fastq/1kseq_R2.fastq.gz"
adapter_path = "../test/adapter.fa"

ATrim = AdapterTrimmer(PairwiseAligner(), adapter_path, min_size, read_len)
QFilter = QualityFilter(min_qual)
FFilter = FastqFilter(QFilter, ATrim, input_qual)
print (FFilter.filter(R1_path, R2_path))









    



	Extracting data
Uncompressing and extracting data
Parsing files and filtering sequences

Number of sequence analyzed :

1000
====== FASTQFILTER QUALITY CONTROL ======

  Execution time : 9.86074185371 s
  R1 file : ../test/fastq/1kseq_R1.fastq.gz
  R2 file : ../test/fastq/1kseq_R2.fastq.gz
  Total sequences processed : 1000
  Input quality score : fastq-sanger

====== QUALITY FILTER ======

  Quality Threshold : 31
  Fail quality filter : 616
  Pass quality filter : 1384
  Mean quality : 30
  Minimal quality : 32
  Maximal quality : 31

====== ADAPTER TRIMMER ======

  List of adapters imported for trimming
    Name : Illumina1	Sequence : ATGCTGCTTA	Times trimmed : 57
    Name : Illumina2	Sequence : TTATCGCGTA	Times trimmed : 5
    Name : Illumina1_RC	Sequence : TAAGCAGCAT	Times trimmed : 62
    Name : Illumina2_RC	Sequence : TACGCGATAA	Times trimmed : 29

  Sequences untrimmed : 836
  Sequences trimmed : 112
  DNA base trimmed : 5632
  Fail len filtering: 51
  Pass len filtering : 61
  Total pass : 897

====== PAIRWISE ALIGNER ======

  Parameters of pairwise2
  Maximal number of alignement : 5
  Match bonus : 2
  Mismatch Penality : -2
  Gap open Penality : -2
  Gap extend Penality : -2
  Score cutoff : 1.5

Still working after splitting.

2014 06 27

Test of BwaWrapper Class



In [2]:

    
from ShortReadAligner.BwaWrapper import BwaWrapper
bwa = BwaWrapper("bwa", "../test/references/all.fa")
print (bwa.index_report)
bwa.align("../test/fastq/1kseq_R1.fastq.gz", "../test/fastq/1kseq_R2.fastq.gz", "1k")
print (bwa.align_report)









    



'./bwa_index/' already exist in the current directory
Indexing all.fa.
[bwt_gen] Finished constructing BWT in 5 iterations.

[bwa_index] Pack FASTA... 0.19 sec
[bwa_index] Construct BWT for the packed sequence...
[BWTIncCreate] textLength=64256, availableWord=82138
[bwa_index] 0.00 seconds elapse.
[bwa_index] Update BWT... 0.00 sec
[bwa_index] Pack forward-only FASTA... 0.00 sec
[bwa_index] Construct SA from BWT and Occ... 0.01 sec
[main] Version: 0.7.5a-r405
[main] CMD: bwa index -a bwtsw -p ./bwa_index/all.fa ../test/references/all.fa
[main] Real time: 0.634 sec; CPU: 0.212 sec

Aligning reads against all.fa with bwa mem
[bwa mem] Finished alignment

[M::main_mem] read 2000 sequences (300000 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 425, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 348, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (359.03, 114.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::worker2@1] performed mate-SW for 43 reads
[M::worker2@0] performed mate-SW for 42 reads
[M::worker2@2] performed mate-SW for 37 reads
[M::worker2@3] performed mate-SW for 44 reads
[main] Version: 0.7.5a-r405
[main] CMD: bwa mem -t 4 ./bwa_index/all.fa ../test/fastq/1kseq_R1.fastq.gz ../test/fastq/1kseq_R2.fastq.gz
[main] Real time: 0.144 sec; CPU: 0.151 sec

Genome Indexing and alignment works fine for small size files => Try with a larger dataset in idle

Same thing with the whole human genome = Works fine with on genomic size sequences.

Using PIPE and Popen I think it may be possible to redirect bwa stream directly in pySam.



In [1]:

    
from ShortReadAligner.BwaWrapper import BwaWrapper
bwa = BwaWrapper("bwa", "../test/references/all.fa")
print (bwa.index_report)
bwa.align("../test/fastq/100seq_R1.fastq.gz", "../test/fastq/100seq_R2.fastq.gz", "100")
print (bwa.align_report)









    



'./bwa_index/' already exist in the current directory
bwa index -a bwtsw -p ./bwa_index/all.fa ../test/references/all.fa 
Indexing all.fa.
[bwt_gen] Finished constructing BWT in 5 iterations.

[bwa_index] Pack FASTA... 0.00 sec
[bwa_index] Construct BWT for the packed sequence...
[BWTIncCreate] textLength=64256, availableWord=82138
[bwa_index] 0.00 seconds elapse.
[bwa_index] Update BWT... 0.00 sec
[bwa_index] Pack forward-only FASTA... 0.00 sec
[bwa_index] Construct SA from BWT and Occ... 0.01 sec
[main] Version: 0.7.5a-r405
[main] CMD: bwa index -a bwtsw -p ./bwa_index/all.fa ../test/references/all.fa
[main] Real time: 0.559 sec; CPU: 0.015 sec

Aligning reads against all.fa with bwa mem
[bwa mem] Finished alignment

@SQ	SN:rAAV_RSV_GFP	LN:2216
@SQ	SN:Bacterial_backbone	LN:2811
@SQ	SN:Helper_plasmid	LN:22757
@SQ	SN:Ad5_in_293_genome	LN:4344
virus|00|0|1-150=AAV_RSV_GFP:1922-2072	83	rAAV_RSV_GFP	1923	60	150M	=	1406	-667	AGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATTTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTCAAATGTGGTAAAATCGATTAGGATCTTCCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTT	:7?>@<0)4F<5DEF;>67=6B::<;@C<ICIAII7;@?DF>?<GD<III94833@ABIIB=?>:0;<;?==>=@?<A@>CE>?BFCCB@BCECCCDCDDAAEFEFCDCBEFFGFFHCB@@DDDCDEFEEEGIFHCG?AABED@@@>?;<	NM:i:1	AS:i:145	XS:i:0
virus|00|0|1-150=AAV_RSV_GFP:1405-1555	163	rAAV_RSV_GFP	1406	60	150M	=	1923	667	TCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGCGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAATGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGC	EB=C<>=@A?BDGBEEEACEDDEDDCDDEACGFFEEIIEFEEFFDEEGHFEFFCCCCECC@C?A<>BF@EE@?B?@CBEIEIBCAA@?@@EA@A99C:;BE9CDIIGCHICC>927D934138?ICAFII<=7><5052695;68B/440	NM:i:2	AS:i:140	XS:i:0
virus|01|0|1-150=AAV_RSV_GFP:28-178	99	rAAV_RSV_GFP	29	44	150M	=	210	331	CCCGGGCAAAGCCCGGGCCTCGGGCGACCTTTGGTCGCCCGGACTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCCGCATGCTACTTATCTACGTAGCCATGCTC	B?@@BG@A=AD@@DC@BDD>CBBCBDDDDECBDDACDFEEFFECDEEFEGFEBAGCCGIHD@C@A=@A@<79<AAA=BE?9=8@D=;7<19@@GEI?A??51.:34<;97/<;89;;5BDC<6F@<E@4/@><2)3,39:5BHDCAEI5<	NM:i:2	AS:i:140	XS:i:133
virus|01|0|1-150=AAV_RSV_GFP:209-359	147	rAAV_RSV_GFP	210	60	150M	=	29	-331	CTTATCATCGCAGATCCGTATGGTGCACTCTCTGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCG	37;5?=9>8A<::B6>>6=:?=;<1=:;8:1/8=C>72;2768>EGE;DEAEC84=:47:9=@G<?>CAA;;>CB>@@CFHEFHDEDDEG@?CFFIGIEBCDGEFEEFFC@@DDFHGIGBABEEEIGCACDDEEDCC?ABCAAAAA<B?C	NM:i:1	AS:i:145	XS:i:0
virus|02|0|1-150=AAV_RSV_GFP:1012-1162	99	rAAV_RSV_GFP	1013	60	150M	=	1223	360	GGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCA	?<BA?BC@=<ABD?ABBEGHEFECDDBDDDDBAEEEDDBDEECHDFED@BDCCEEBCEEDDFGEEA?>GICADGIIE?A?BEIIIB@<89=38<?F9;@<?IIIDHHI@C<BII=@>ACEA9748>;?9<66?CA79DC?526/4<D?AG	NM:i:1	AS:i:145	XS:i:0
virus|02|0|1-150=AAV_RSV_GFP:1222-1372	147	rAAV_RSV_GFP	1223	60	150M	=	1013	-360	GCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCG	@;:<>50-A3-205119>?>D<>?@>=>=>9:8>AIFB<B>33BC>C9-0EDCG=@@99;>E@3:<AACEIDAB<>;:I@DCDD@ADEDD@BCEGEEEICBA?CFDCBCDBABCADFHFGHFEEBDC@>?DEEF?BA>@GI>A=<A@BB?	NM:i:0	AS:i:150	XS:i:0
virus|03|0|1-150=AAV_RSV_GFP:1558-1708	83	rAAV_RSV_GFP	1559	60	150M	=	1329	-380	CCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA	I20,*-400:@EE6==:4EC@?B/2*.7:19;ABA?@:<:;;7>A7??IG=>@FE>::@;BFCFD?@ADFIGGAABAA?CA?AAABACCCCCHEFEFHDGCDDFGDEFEDFEGEEBCAEFHHCDDCBBDGCGEFGCHHBFDG@@<>=<55	NM:i:0	AS:i:150	XS:i:0
virus|03|0|1-150=AAV_RSV_GFP:1328-1478	163	rAAV_RSV_GFP	1329	60	150M	=	1559	380	CGACGGCAACTACAAGACCCGCGCCGAGGTGCAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATAT	>=<>ACCCGFFABGGFDEEHFFEGGGGFEDDEDCHCBHGCHFEECDACADGEGEBCCCE@@;:?AC>DC@AAA?:8@AD>@C?FEBFC?C?A<BA:A@ICA8EIIEDE:=A?>DH@>><?C@HBB=9-*?G<:<067A;>C091*)(#,&	NM:i:1	AS:i:145	XS:i:0
virus|04|0|1-150=AAV_RSV_GFP:1174-1324	83	rAAV_RSV_GFP	1175	60	150M	=	1001	-324	GCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCA	3;B3450*10D:>;6/&;1;A<HC9>:@=G?A==?C<EIA>?AHIDB5;?EFEIIIIFBI@A:F?@GD<9IIH<9BEE@<?<CDHCCDEGFCCFGCEEABEFHGFFDEDDEDEFGGFECEDDDCDEDEEFGEECDHEFE@?<>@?B><?@	NM:i:0	AS:i:150	XS:i:0
virus|04|0|1-150=AAV_RSV_GFP:1000-1150	163	rAAV_RSV_GFP	1001	60	150M	=	1175	324	AGCTGCTGCAGCGGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGCCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGA	>@??CD?A?>?@?ABCFDCCDFBAIGDBCDCDCDCEGDDCBCDCCDGIDEIIGGFCCCCHGEDEDGC=;9;AAFGHEDHAH>AA:><IDEIE?><AFEID><0-6,4A<;544?=B:48?FA?76;8@>04;.833B7=<:;54035?E5	NM:i:1	AS:i:145	XS:i:0
virus|05|0|1-150=AAV_RSV_GFP:605-755	99	rAAV_RSV_GFP	606	60	150M	=	696	240	CCAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCT	??=><@BAB@@A=@@=ACACFEGBCABFIHGFFFFEDBDDDDCADDFEGGDDBBBAA=>?A@@@C@>=??>AFIEED?ECB@?=61HD>>G?@D;FII@AAC?D;840<:<?<?=><6016DIC-<<8:;A-6;7429;@=?<:=954:6	NM:i:1	AS:i:149	XS:i:0
virus|05|0|1-150=AAV_RSV_GFP:695-845	147	rAAV_RSV_GFP	696	60	150M	=	606	-240	ATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGATCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCA	508988;:B2DGF>-,,=9;97A266A7?/<?<>255.<:7;8B@@@AD<??916=>=IBI??D:CFCGIACEDCBGGACCCDDDAACCBABEBCDCDCDCBEFCA?FEDCAEHFGGEEFGEDDDECBAEECB@DCB?@CB?A?ABABDB	NM:i:1	AS:i:145	XS:i:0
virus|06|0|1-150=AAV_RSV_GFP:1897-2047	99	rAAV_RSV_GFP	1898	60	150M	=	2067	319	TAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTCAAATGTGGTAAAATCGATTAGGATCTTCCTAGAGCATGGCC	?A?@BCBBCD@AAA?@ABEEEGBCDD@CADDCEBCDGFEGFEEEGFEEEDCDGFEEDCEIHA?BBBDD?AABBFIH?>BBCBC@<C>=;><:DI>?:47GE??ID8=2?=>;8?A93;38.>I9;I9=;?7338123B720+372?<@,8	NM:i:1	AS:i:149	XS:i:19
virus|06|0|1-150=AAV_RSV_GFP:2066-2216	147	rAAV_RSV_GFP	2067	40	150M	=	1898	-319	CGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAG	9IH??9:?7@?:==;4:>9>@F?C=>;DA@A@A82/+.2747?:=;77<<FIIIEIIF>?BEE9?FHDIE9EFD@<AA;=A@=@AEBAD@DCBECCADDGFFEEEDCBEFECBAAGCEDHGHHIHHCBC@BCBCDDDABBA@C@=@>>B;	NM:i:0	AS:i:150	XS:i:150
virus|07|0|1-150=AAV_RSV_GFP:767-917	99	rAAV_RSV_GFP	768	60	150M	=	1019	401	CGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTG	=>>@?>CBDDE?DAADDCBFEEDGFEEAD?EIHFGFCDDDFFCCGFEHGIIFECGBDCBIDCCFD=@A?FFD@=><BFAGD<?<@>?:>?D?=?CIH>IIFA8<@<AFA<DB<<@?7A=H@@GIHI>>HB5.+6,256.+3'6/378E<<	NM:i:0	AS:i:150	XS:i:0
virus|07|0|1-150=AAV_RSV_GFP:1018-1168	147	rAAV_RSV_GFP	1019	60	150M	=	768	-401	TCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTACACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCCGACCCTGAAGTTCATCTGCA	A3*1AH@7B5/6394;;;>7?<91?;<B9:E=;;.-5:49F8?70828>=<AEA9?:<@ID?FBC;>;CIHGCBCICB:ABB@CCDG@<CBCECCEEF@@CDFCBEECGCCBFGIECDDDEFFGFGGFFDEDDEB?A?BDDEBAA@A@<9	NM:i:2	AS:i:140	XS:i:0
virus|08|0|1-150=AAV_RSV_GFP:754-904	83	rAAV_RSV_GFP	755	60	150M	=	615	-290	TCGATACAATAAACGCCATTTGACCATTCACCTCATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATA	CD98A51(>;7;;65;6=69;:;<<IA>GII?><<ECHF68;52:087>?<;=5;5B9DFB77=<:=BI<9:;=>@?A9:39GEFEC@DAFBAFDDCAB@DFFGEEEFGCB@BEAEEEDGIGEEDCBDFCAECBEEBA<<??=@AA;;=8	NM:i:1	AS:i:145	XS:i:0
virus|08|0|1-150=AAV_RSV_GFP:614-764	163	rAAV_RSV_GFP	615	60	150M	=	755	290	GAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGATGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAAT	AC?>A=CDCB=CB?AABCEFDDDHHGIFGFFDFFFEGHGFEFFDECCDEBAACFDDGHFEDGDDCDDB>>CD=CBEIG<IIDIIFC@<FGFD@@AID=>GC==6:<>8?7B?>8022A<@C@52792'49;?51=98<?7A;=6=862;5	NM:i:1	AS:i:145	XS:i:0
virus|09|0|1-150=AAV_RSV_GFP:676-826	99	rAAV_RSV_GFP	677	60	150M	=	778	251	GGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGC	@?C?DEHC@>@@@BACFDGBCDDDDDCDBCCGIEEEBBABCAEEEEEDFFFEGHCCDG@ABCCCCBDCB?FIBBA<B>AB@EFA9==HEBFIFFGDIID:3FCIDIG>7>7=?BC@A8>?C:;:C<:;486@7B;ED<8?:;H>;=7865	NM:i:0	AS:i:150	XS:i:0
virus|09|0|1-150=AAV_RSV_GFP:777-927	147	rAAV_RSV_GFP	778	60	150M	=	677	-251	CCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGA	9D:8:448A<HA8FHFA1=A65:9DC@=G<;E??DB0==533507<256@B?<=@;IDC8@@@?G<<<>A:?CBD@<=AHFFECCBFGBAC>DFDDDDDDFFDDDEEFCFFDGGDDDAFDDCBBEAEEBDEEEEFGHFB@:A?=:=>=<8	NM:i:0	AS:i:150	XS:i:0
virus|10|0|1-150=AAV_RSV_GFP:1840-1990	99	rAAV_RSV_GFP	1841	60	150M	=	1998	307	GAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTACATTCATTTTATGTTTCAGGTTCAGGGGGAGACGTGGGAGGTTTTTTAAA	C:;=@@@>?<?ABCABFEFBFEDDEFEDCB>BCFGCDIFCBACBCGFBCEDDDBFEC@D@CC?BGEDBIF>=CDCBCHC@9:=;@<?8;>E>:BBIEI>>DB??<<167D;@>=7>ACIE8H8>609296=<CII?5C?<9&55G=891>	NM:i:2	AS:i:140	XS:i:0
virus|10|0|1-150=AAV_RSV_GFP:1997-2147	147	rAAV_RSV_GFP	1998	60	150M	=	1841	-307	AAACCTCTCAAATGTGGTAAAATCGATTAGGATCTTCCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGC	;G?4;DE765=<;::0656697CGDDCIIAACAA::7G?@;=FIGGGDD=E>?>CI@:9:;FIIDEA?=A>=?C?<<7>FDBC=B==EFDBA@BGDECDCFHEECFEDCDGEFDDHFEEABBCBCBAHIHDFGGIGEDGFCD?=>AC@B=	NM:i:0	AS:i:150	XS:i:112
virus|11|0|1-150=AAV_RSV_GFP:1746-1896	83	rAAV_RSV_GFP	1747	60	150M	=	1386	-511	GCCGCAGTTACGCTATGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT	.=*(,1.-803<GB=3>G?78?4+);9B:>93=DFBCB>AE@I:@CDF?>BDH:IGG87CBDICB<>;B;C@EHCEGDBBAA=>:@?AA@B@@CDECDFEFHHHGEFIEGIDDC@DDFGECBEDAABEFGEGCEEFA?BABCD?>??=<B	NM:i:1	AS:i:145	XS:i:0
virus|11|0|1-150=AAV_RSV_GFP:1385-1535	163	rAAV_RSV_GFP	1386	60	150M	=	1747	511	CCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTGAAGATCCGCCACAACAT	CEA@<:==C@;?>>ACCDBCDEHFEEDDCDEFEEB@BEFGED@CDEDEHIGFBCDDECCDBFDDHFHBADABC??@A:?8@?F>B?;9:D=?B?8EG97<5@<=ADA>9>BFHE7;G>0@<F3>G9.:@;36B215A</:?<?IA@<@II	NM:i:1	AS:i:145	XS:i:0
virus|12|0|1-150=AAV_RSV_GFP:1012-1162	99	rAAV_RSV_GFP	1013	60	150M	=	1237	374	GGGAATTCATAGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCA	?@@@D?@@?B==BCCD@ADBCDDDEIHFHGIGFACEHECCDDEGFCBCACDCDBDDFHFFE@CFDFDEDI<<;A=?BC<==DIFEEABBC;<68<BED=H>AI;=<A@??A?C9<?78GB6:=:/1:<>;1/4=96=<@??042)-5053	NM:i:1	AS:i:145	XS:i:0
virus|12|0|1-150=AAV_RSV_GFP:1236-1386	147	rAAV_RSV_GFP	1237	60	150M	=	1013	-374	AGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAAC	306)22159ADB<C<;<C>32-1=AGI>84@74<<:7?89+4@C7>>=9A?AEDEEA@<C;@@FIBA@@@D==E>CC@>BEGBFHIGDEB>?BAEFDDDCEFEFC@@@@@DDBABCCDGGDDEEFEGGDDDCFEEBBACB?>=?>;89B?	NM:i:0	AS:i:150	XS:i:0
virus|13|0|1-150=AAV_RSV_GFP:1168-1318	99	rAAV_RSV_GFP	1169	60	150M	=	1322	303	CCACCGGCAAGCTGCCCGTGGCCTGGCCCACCCTCGTAACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGGCTTCTTCAAGTCCACCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCT	<=>AGFCABACDDIEEFABCFBACEEEAABEDBCDGDEEHFDDDCDACDCEFBDEBCBBBDEBCEHC>8=?GBHEEC?<@DADA@II@@A<E:9<@BB@?A@=:>@CAA?59717:3383;F>:=/9,8A5C4;=:@@9DIBDB>9779>	NM:i:4	AS:i:130	XS:i:0
virus|13|0|1-150=AAV_RSV_GFP:1321-1471	147	rAAV_RSV_GFP	1322	60	150M	=	1169	-303	TCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCCACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCGCAACG	0C;;;:/+5=CIDIF>;<@BA2)+)48>AF<?;DEGHD:7B324-<3>>=DAIA=:9@;6:968;031<;<BCBB?HE?BDDEBDEEFBEGDGEEICGDDDCBECACHCA>DDCBADDCBCDFGCBCDADDEFDFHCBDDDA@A=;AB>C	NM:i:2	AS:i:140	XS:i:0
virus|14|0|1-150=AAV_RSV_GFP:772-922	99	rAAV_RSV_GFP	773	60	150M	=	894	271	TTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAG	AB;<<??=@C<>?@BBACEEHBCFEFFCCDCF@BCDDCCHFEEEFEDFBCFECCFECHIFGGFE@AACED@7=?@?BDCDGBIIHCCIF=CB@D:C@950AD@GB?HGGB>4>>E<>I<9=74/6;9E97GG83817894/73<7-30:6	NM:i:0	AS:i:150	XS:i:0
virus|14|0|1-150=AAV_RSV_GFP:893-1043	147	rAAV_RSV_GFP	894	60	150M	=	773	-271	GGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGTACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGCAGCTGCTGCAGCGGGAATTCATGGTGAGCAAGGGCGAGGAGCT	3<4<=@?7859AD@B::AG86@9@=A:4@4=;>EGBA:?@E<BD>1248????BA@BBD<9>=>BCEIDGICDBE@?<AAEDEIBBAEFCAFHEGEFCBDDDADEFDDEFHIEGEEFFDDDC?CEFFGDFGDCBAB@A?AEEC@?<;=>@	NM:i:1	AS:i:145	XS:i:0
virus|15|0|1-150=AAV_RSV_GFP:715-865	83	rAAV_RSV_GFP	716	60	150M	=	358	-508	AATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGT	>:7627:;BI?:7;??E<94<FDI=?C:<A:==<@=<69B;@@E:>;I:>C=;=>?EEAF@IIBBDFHDE@??=@A@HHH?GGCCDFEEDDGIHGIIDDDDGEDAADDCADGIGCCEFCDFDDCDDDDEDDEEFCBEFIEACACD@@?=<	NM:i:0	AS:i:150	XS:i:0
virus|15|0|1-150=AAV_RSV_GFP:357-507	163	rAAV_RSV_GFP	358	60	150M	=	716	508	CGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTCCGGGCCAGATATTCGCGTATCTGAGGGGACTAGGGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGAT	98@A;>@=?<A?EIDGFGEFCDDBHHAFDBFFEEBBABFGFEED@CCBCEEFEEDDDFIHFEFFHCC@;7;<A=CIFHBCBI>?3:>DFADDBI<<577B84DDE;>F8;<8C;7>B?<@4<;@9<??5735;B;;80>3;4?G??>B2/	NM:i:1	AS:i:145	XS:i:0
virus|16|0|1-150=AAV_RSV_GFP:2045-2195	83	rAAV_RSV_GFP	2046	49	150M	=	1848	-348	CTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAAGAACCCCTAGTGATGGAGTTGGCGACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTC	@=;8D@99DIIC<;>A=:9:BECIFHG=1(/8@:;4B==IHFEC;BAC>GIBCBE=2>IEA6:A@@@>>@I>=8=;>AEBCCFDGGIH?CBHDDEDFIGEEEDDEEEFEGGDCDDDDDCECEDCGIIEEBCECACBA?<?@AA=A?=?@:	NM:i:2	AS:i:140	XS:i:132
virus|16|0|1-150=AAV_RSV_GFP:1847-1997	163	rAAV_RSV_GFP	1848	60	150M	=	2046	348	GTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATATACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGCAAGTA	???7>@CC=FAFCC@D@DCDCGEGGIGGFEEFEEFCCDDCABBBCEEGFDFFCBEABDDGBBCCBBB?;FCD?8@B>?HHE=DDIIDB:@HACABA<9=;<CIIHGF99@AHI58E9IIBI>DIC246EE@;>?F407@DH>@56?3)*4	NM:i:1	AS:i:145	XS:i:0
virus|17|0|1-150=AAV_RSV_GFP:755-905	83	rAAV_RSV_GFP	756	60	150M	=	482	-424	CGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAG	;6E?87632:G<5??9=2.>@@;5@FF>?D>C<276;3A6<30;:><;:GEIE?AFB;6=I?@=><DCA:=<<=74:=CCDD?@ACFECBGGCCDEFDCGDHEHEDEDHGCBDFABCCCABCFEECBDACBCCD@>A@BB@DCF@;==?:	NM:i:0	AS:i:150	XS:i:0
virus|17|0|1-150=AAV_RSV_GFP:481-631	163	rAAV_RSV_GFP	482	60	150M	=	756	424	TACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACGTGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACGGTGCAT	?>?@@=A?DDACCB<=ABBEGFEEDCDEFFDCEEDDDCBFGFEEGFDDCDEFDDDEE@AD=EFCB@A=>CG?D=?>?BCG>7;>H@B?I??CA;BCGCDA:;5=:GBAI<7D3@:/6<8:<:=8>G;<AH=@DA9<48/;?<<1=.8?=>	NM:i:2	AS:i:140	XS:i:0
virus|18|0|1-150=AAV_RSV_GFP:1491-1641	83	rAAV_RSV_GFP	1492	60	150M	=	1253	-389	CAGAAGAACGGCATCAAGGTGAACTTCCAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGTCCACTACCAGCAGAACACCCCGATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCC	3-0623=/697;14CH9A:2=;<<=?87509AA?:CAD<>>)40694+:5DIB>FCFDA<D@=?E?ADEF@@BBFCHIACADCDB@GEGDECDBDEEEEFDEEFFGEFGHFHHGFEFECEABDFFEIDDBDDEGDC@A?AEB=::><>:7	NM:i:3	AS:i:135	XS:i:0
virus|18|0|1-150=AAV_RSV_GFP:1252-1402	163	rAAV_RSV_GFP	1253	60	150M	=	1492	389	ACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGG	==@AA=@@ACBCCBCCGFEEEEFECBCDDCCDBECCCGFGHHECDDDDFGFEEEEAAABBDEDIFGEEB<:@DDICBCBD>;B;F;>>B?<@I@?4:<@?EB72/3265<FC@?@FCB@I9HB;A?8,52030/1+/.A::<:9>6%/(2	NM:i:0	AS:i:150	XS:i:0
virus|19|0|1-150=AAV_RSV_GFP:1723-1873	99	rAAV_RSV_GFP	1724	60	150M	=	1806	232	ACGAGCTGTACAAGTAAGATGCGGCCGCAGTTACGCTAGGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAA	?:>??BAA?DDFDDEDAC?CD?@@EEEBCFGFGFBBDBCFEEGFECDDDDDDHEEDDBBFCHCFBCAD@?AC=::EEAD:9;AFIEB?;>HI>:BHGGFGIDAIDADAEFCAGAI4?=D=0B10404128>39=<:;C>6*6<3HIA965	NM:i:0	AS:i:150	XS:i:0
virus|19|0|1-150=AAV_RSV_GFP:1805-1955	147	rAAV_RSV_GFP	1806	60	150M	=	1724	-232	AAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTT	;D=88/4<@>5<?9<8/4<><==?BC9<<I<7<:@HD==I>B?AA>BG?;88;3;ECBE?:<7HIGGI=6;@A@FCEA><@;>@CAD?A?A:B@BBDFFEFDB@EDADDDDDEDEDDCDCEIGEDCDDECFBDFE@CEA>AA=@<BE?CH	NM:i:0	AS:i:150	XS:i:0
virus|20|0|1-150=AAV_RSV_GFP:1769-1919	83	rAAV_RSV_GFP	1770	60	150M	=	1676	-244	AGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGTCAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAA	87=?:D>;4?CC;7-<97?B6%&$4:<;>9;<;FF@C>?@AB<278;=@BCHBAAIFIIBIE>C98A<=>GFE?;BAEDBBIEEBCCAAEFFFDDBEFEFADCDGDCBDCDCBDDDFGDECCEFGIFFDEFCBEDCDCB>@>BAAD><<=	NM:i:1	AS:i:145	XS:i:0
virus|20|0|1-150=AAV_RSV_GFP:1675-1825	163	rAAV_RSV_GFP	1676	60	150M	=	1770	244	TGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAGATGCGGCCGCAGTTACGCTAGGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTG	?@CA:B=@DD=@DCGECGFECEGHEDDFFEEGCFEEEGHFEIHHIFECDBBDDCBDABBEGFAHEFDI@<AI@CC;?7B?AB<?=;BC:AGB:=F>A;F?I?GCCDAA<=7=FIAAIDA=:8;>II<AEC?;6>>A@7317,:43=857=	NM:i:0	AS:i:150	XS:i:0
virus|21|0|1-150=AAV_RSV_GFP:938-1088	99	rAAV_RSV_GFP	939	60	150M	=	1170	381	CGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGCAGCTGCTGCAGCGGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGT	@?;:<><BCBAEDD??DDEIGEEEDFHCBCEEGFECBBB@@BDECDABBCDDEEEDGHFAA@=<?ADDCA:B@FBABC@?A><DCICBC@DFB?BGACA?=:B==AC752/75A<8E@E@?9<@;4-56>G@>EIIA@:8><BAB972*9	NM:i:0	AS:i:150	XS:i:0
virus|21|0|1-150=AAV_RSV_GFP:1169-1319	147	rAAV_RSV_GFP	1170	60	150M	=	939	-381	CACCGGCAAGCGGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGTAGGCTACGTCCAGGAGCGCACCATCTT	686F<25*.0@6;;<6=C=?C9;9BD63/1DD=2><;C>9AIB==A@?ID89;A@F?ID?@=AGEHH=ACABEID??>??IACBE@BHGEBDDEEADDEECB@ACE?BDDFCFGCDDDDDDHGCDDEGDEDCBDCA?AF?AAAA=;9?=>	NM:i:2	AS:i:140	XS:i:0
virus|22|0|1-150=AAV_RSV_GFP:450-600	99	rAAV_RSV_GFP	451	60	150M	=	692	391	TGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACAT	?B>@A==?EDIEIHIIGIB@BCCDBEDBCCBADFFHGDCEEEEEEEFEEABCEEEDCBCFIDEEAA@?BA>@=?AAA79BIE=9=?>?A6;?@BAF?@=?>=>0>?AA<BC8DAC@HEED@5>98B5061;==B9IA><18CH>@?2861	NM:i:0	AS:i:150	XS:i:0
virus|22|0|1-150=AAV_RSV_GFP:691-841	147	rAAV_RSV_GFP	692	60	150M	=	451	-391	TGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGC	<:8B55.59953AB2+,:0:44;5%:;;=>@<:8F:75I?D:?FII=BII=<02=@ABE89628?B=:BAGGFAACC=<BBGFBBBDDCACBBCCEFFGGGHIGEDHECBCDFCDDCBDDDEFECBCDCDDB???B@CCBB?CEE>=;?@	NM:i:0	AS:i:150	XS:i:0
virus|23|0|1-150=AAV_RSV_GFP:1455-1605	83	rAAV_RSV_GFP	1456	60	150M	=	1232	-374	TACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTG	6D@A::?C4?2@E@;<D<:@3>840-@79<6B?BB8:=>@@E@EB?8@AH:@>DFE>==?DBBCHGAFBG<8:AGDA@=EACDFDF@BCDAEECCDCGEEC@DCEDEB@@CBDFDDCCDHGGEGFCDCDCBBCA=DECDDAD??7;A@@>	NM:i:0	AS:i:150	XS:i:0
virus|23|0|1-150=AAV_RSV_GFP:1231-1381	163	rAAV_RSV_GFP	1232	60	150M	=	1456	374	GCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGAGCCGAGGTGAAGTTCGAGGGCGACACCCTGG	@=<?@@C==>CDDBC@BBBCCDECABCEEEDBBC?BEDFEFBCDECEAFHDFHFFEEGIDBDDHI?BACC@>>BDDCDD>BBFII>?:?><:I=;C?C>>=39@A@;?C==99><?<D=606G;B+58C43D44=@?::?<02:A<7E@C	NM:i:1	AS:i:145	XS:i:0
virus|24|0|1-150=AAV_RSV_GFP:538-688	99	rAAV_RSV_GFP	539	60	150M	=	712	323	AAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGG	AA?A>@<=?@DDFEGGGFBCCDDECBCAFEEFDDEFECDCEDEDFECFFFDEEEGEBCCCCDCFDEDCCAAFDBAEBC@6=7A3>AEII=??A@@BDDH>DADCIE49;DDEBAFC>FHG=1606>0:=6<<7:@=@=C?71879*2;E?	NM:i:0	AS:i:150	XS:i:0
virus|24|0|1-150=AAV_RSV_GFP:711-861	147	rAAV_RSV_GFP	712	60	150M	=	539	-323	ACTAAATTCCGCATTGCAGAGATACTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCACCTGGAGGCACTGGGC	@:;ECC6459F;G:3:4:H7HE;GFBD=8A;8AF?983<?F@??@@B=;<@B9CICCDG;<>;@G>CFFGA?9?BBCBA><>BEHDEDDDDEFDDBEFEDDDFFEBCEEEEECBDEEEFCFFEFDCEEDFEEDB?@@BA=@>?>@?>=@F	NM:i:2	AS:i:140	XS:i:0
virus|25|0|1-150=AAV_RSV_GFP:2066-2216	83	rAAV_RSV_GFP	2067	40	150M	=	1898	-319	CGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGAGAGCGAGCGAGCGCGCAG	CC>==<7<9'6-434B74/:7BB?IIG8;EGCI?;46B5<EE>=;C73@>>/?<7:DBD=89C?F@C=B:=<8?A@A@>DADECDEAEFIFCCBBGCCEEFDCFGDDHFFBCCBFDDDDHEEEFGFEFHHHIGHCCB?@@CA>BC::>=?	NM:i:1	AS:i:145	XS:i:145
virus|25|0|1-150=AAV_RSV_GFP:1897-2047	163	rAAV_RSV_GFP	1898	60	150M	=	2067	319	TAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTCAAATGTGGTAAAATCGATTAGGATCTTCCTAGAGCATGGCT	@=A@?B@7;>?AEDBDEDEGEEDD@CDCCDBGHIEEDDDCCDEEDEBCC?@DBEGBC@@@EDFHGECDIIGCAA=DDIIEAIDIHHECC:<=CII8;:>;?AA>>?7?AD;8?IF;<48B5?E>3-4C=?@>:4D89CE868.023&'1*	NM:i:0	AS:i:150	XS:i:20
virus|26|0|1-150=AAV_RSV_GFP:370-520	83	rAAV_RSV_GFP	371	60	150M	=	194	-327	AAGAATCTGCTTAGGGTTAGGCGTTTTGCGGTGCTTCGCGATGTACGGGCCAGATATTCGCGTATCTGAGGGGACTAGGGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGC	=335=AA=6<H?EGIE?:AIBI8=EBB=I@<;BG=B:6ABD9A@A946?@<IE9B@EH?B603885;;EIEIIEHAEBB;;DFIBFADD?CFHEFIIFFHEEFFFFGECDCBBDDBBBEEDCCEECCABBDDGGGB@EEEFBCA??@<DA	NM:i:1	AS:i:145	XS:i:0
virus|26|0|1-150=AAV_RSV_GFP:193-343	163	rAAV_RSV_GFP	194	60	150M	=	371	327	GCGTCATGTTTGACAGCTTATCATCGCAGATCCGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGATGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACA	7:?A?=?@A@ACCC=BAACDD?CDDDEEGHDADDECHFFABBCEGIGFDAECCHGED=BCFC>BCIDCAAB=@?DAD@E4>?@HIHI=@@DBI81;<AC?=FCCAI;;:4=B?><=>EA@@4:HB?G=GIBA:9=>6AI>=IIA;@A=B?	NM:i:1	AS:i:145	XS:i:0
virus|27|0|1-150=AAV_RSV_GFP:121-271	99	rAAV_RSV_GFP	122	60	150M	=	449	477	GGGGCTCCTTGTAGTTAATGATTAACCCGCATGCTACTTATCTACGTAGCCATGCTCTAAGAAGATCTCGACGCGTCATGTTTGACAGCTTATCATCGCAGATCCGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTA	A@BCB>@??D><AEEIHFCGHFDFFGCEECDDCFCEDBBBBCDFEDEFCECDECCCCEFCEDDIHEB?;BBBDC>=>>@5?0?C><:@>4ABCHBI279@;AI??BB5;=?F:H@@DE;535DA692?;;<;B8AE;0;7/162:1:89A	NM:i:2	AS:i:140	XS:i:50
virus|27|0|1-150=AAV_RSV_GFP:448-598	147	rAAV_RSV_GFP	449	60	150M	=	122	-477	GGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAAC	.1=@3<=5:>673:D;E=9BIG@EE0>8:71904?<@B7=6@802CBAIECHE=?@B<;DIBCIE>>7<@?CE;>CDG>DDFDEHECGBDEGDABCEEDADIFFFEEEEFEFEECDCCFDDFGEDDCDDDFEEGFB@<;A?<@@EEB@BD	NM:i:0	AS:i:150	XS:i:0
virus|28|0|1-150=AAV_RSV_GFP:1183-1333	99	rAAV_RSV_GFP	1184	60	150M	=	1308	274	CCGTGCCCTGGCCCACCCTCGAGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACG	:<DACBD>B>@ABCCBBCDEDFDDCIFDFDCDEFDBABEDDBEEFFFEEHGIEFGDEEFHGIFGAA<=C@=EFDFFDDCGGDD:=F:?;=6GBIDIDFIB-2;>FCBGICF>::8:;69=:<IC3:2337=IG@<BE<:490=?D8>0,.	NM:i:1	AS:i:145	XS:i:0
virus|28|0|1-150=AAV_RSV_GFP:1307-1457	147	rAAV_RSV_GFP	1308	60	150M	=	1184	-274	GCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGGGGGCGCCACCCTGGCGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGCCAACATCCTGGGGCACAAGCTGGAGTACAACTA	:B5906@F<4'.1/5?63365*8=;2)=/3;?:IAA;:<:EFIDEIAHII>7;FEI>?CGFIIFIHC><5?<B?<=8<>BABGFACIFDDDEABBDBADBCFFFECBDDFFFFEDECDCCEDCA>ACADDEEDBBCB@<?DI>AAA@CIC	NM:i:4	AS:i:130	XS:i:0
virus|29|0|1-150=AAV_RSV_GFP:245-395	83	rAAV_RSV_GFP	246	60	150M	=	139	-257	CAATCAGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTACATGAAGAATCTGCTTAGGGTTAAGCGTT	0/<8<691)87?=BD9>2:5-.;;>IE??>?E<8?@=;B<>@GIGC;D@E@BEDACA?<@ABCE=?CBADCCDG@?FC?ACCDDAEEGDD@BAADEEEDCDEDDDDDCA@@CCABDCCEB?ACFDEFHCECBEEDCCGICC?=ABBFCCH	NM:i:3	AS:i:135	XS:i:0
virus|29|0|1-150=AAV_RSV_GFP:138-288	163	rAAV_RSV_GFP	139	60	150M	=	246	257	ATGATTAACCCGCATGCTACTTATCTACGTAGCCATGCTCTAGGAAGATCTCGACGCGTCATGTTTGACAGCTTATCATCGCAGATCCGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCC	=A@BDAEECABCIIIIEHHGDB?DEGGFFBEEEEBCGCDCAADEEFEEDCGFGBFGECDDDCE@>FCCIEFDEF9?CB=IGHD?BIICC:BEC>BB<EC>=C8=>H;991<;99AF<>:D7;66?9@5;199<9<04?@;<?=<G;12B>	NM:i:0	AS:i:150	XS:i:0
virus|30|0|1-150=AAV_RSV_GFP:1420-1570	83	rAAV_RSV_GFP	1421	60	150M	=	1139	-432	ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAATGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC	@5::E<1,C=?>+737CE;;899;;3:F::86>E?EIA=<AD<D=EB<8??CF9B7DHBIIEEA?IH?FA?C>@HBC@=;FCEFC@@CCBBAB@BEEEFGGDDFGEFEFEFDD@A@FGEDDDCCGEEGFFDDC@>?<?A?CEAC>>B=DC	NM:i:1	AS:i:145	XS:i:0
virus|30|0|1-150=AAV_RSV_GFP:1138-1288	163	rAAV_RSV_GFP	1139	60	150M	=	1421	432	ACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTTCGCCATGC	<B@>@BB?AAB@>CIHFEEEFC?CBBCD@ACBDDEGFCDGCHIDDEBEHFCDDDDBEGBFEABEICDABH??DIEA><=IEEIE;>;IGB@>E?96?G@5AD=;=@=F?:<928:28GE?IDEC9B?A@88;>GD<55;;95874/"6:>	NM:i:1	AS:i:145	XS:i:0
virus|31|0|1-150=AAV_RSV_GFP:621-771	83	rAAV_RSV_GFP	622	60	150M	=	523	-249	CACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCCATACAATAAACGCT	78<8:.51)92504.?5778EI8;;9=>C9=;8>8;;9;=DH?@B>5?GE>=3=E<;765??DEB@5;<@D@IHEBCDA>BFBACCDIIEEEAFFDCB@DDEGAECBBCDEDCDDC@BDDDCDBAACFEEDDCABDB?AFDB>@BACGEF	NM:i:2	AS:i:144	XS:i:0
virus|31|0|1-150=AAV_RSV_GFP:522-672	163	rAAV_RSV_GFP	523	60	150M	=	622	249	TTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTA	A@?@>CBABGHCCGFDBDDBACGDDCDDFDCIDDEEEGACBCFFCBBA@CCDFCHFFBC@@DDAB??A@FEC@EGGFABIIB=?C==8;AD<A>?@FIGC>=?89>FE>9>?;<A@DF@IGD:HB9@AB6>8>F;AA>-@?C;IH?4>@<	NM:i:0	AS:i:150	XS:i:0
virus|32|0|1-150=AAV_RSV_GFP:646-796	83	rAAV_RSV_GFP	647	60	150M	=	545	-252	TAAGGTGGTACGATCGTGCCATATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGT	287A:597780406=</570B9657=23@6=@>?@:?C7@88DD=A<53<::>CIII8<<;;EIF>;>GB>BFCD@DDEA@?>A?BB?D=BB@DCDCIFECDEDEEBDFEDC@AEDDEEEFDECDBEEAGGEEACBB?@BBBB?>;<:=?	NM:i:1	AS:i:145	XS:i:0
virus|32|0|1-150=AAV_RSV_GFP:544-694	163	rAAV_RSV_GFP	545	60	150M	=	647	252	AGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGA	9;<>BA?@B>BCDEADDFBCDBCCDCDFACHCDEGFFEEEHFEGGGHGFEEFBCDEGFFIIIHEBGE@:>@;>@FEDA4><7<=C@87467=B7IAEFDDB9?:<015>;:=6E6BE@99@@2;3:;<ABE91:5559=98189C0)78C	NM:i:0	AS:i:150	XS:i:0
virus|33|0|1-150=AAV_RSV_GFP:352-502	99	rAAV_RSV_GFP	353	60	150M	=	546	343	TTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATTCGCGTATCTGAGGGGACTAGGGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTC	D:6;BA?A>@:DC?AIHEFFCDFAFEBC@BGEEGEFEFEFEGFDEECECFIGGFAADIHEDBBB>:>EEDABDDD?>@;@>>?;8@IDHG>;5>HDIIHEFEIDII<7=B@@BIBBF9A@:;<8:DC?924:8217?==<9<866DH8;3	NM:i:0	AS:i:150	XS:i:0
virus|33|0|1-150=AAV_RSV_GFP:545-695	147	rAAV_RSV_GFP	546	60	150M	=	353	-343	GTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGAC	7887?7=83/6.245:95.4582<:B/+6;><9=A=@@@EGIHDEFA>>A:BE@:FDA?>DBECDDI@A@EHCD?DGC>A?=A@?EACCCCEEBBCAFECEEDDBDB@@BFFC@@DFFDHFIGIHDDDCAEDDCC@CCCBBBBABB<C;@	NM:i:0	AS:i:150	XS:i:0
virus|34|0|1-150=AAV_RSV_GFP:1257-1407	83	rAAV_RSV_GFP	1258	60	150M	=	944	-464	AAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATC	69C=B994IIE<3.6:IFF?;754,:<E@DBE;>@:?CC?@C2;8>@B=;9IH<7C63@DAFIIEBABA=@>@@?GEGDAA=CFHFEDDDGBDDEFBCEEFGCDDB@EEFEECBDBBDCDDEFFDDDCDDDEDEF@BCCCB@FA=@AC>=	NM:i:0	AS:i:150	XS:i:0
virus|34|0|1-150=AAV_RSV_GFP:943-1093	163	rAAV_RSV_GFP	944	60	150M	=	1258	464	CTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGCAGCTGCTGCAGCGGGAATTCATGGTAAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCTATCCTGGTCGAGCTAGACGGCGACGTAAACC	>@?:?D?A<CBHHHFEDDDBB?BBDBA@BCHDDDBDFECFEDDDDCGHDCDEEEEIFAABC>FED=@AEEBBC=>GIEGAA?@3?95@=C?;:=>A9A@B16764283>EG=5/4:4633:B?;<94<=:8=;908BD5=2@D7AHA>;2	NM:i:4	AS:i:134	XS:i:0
virus|35|0|1-150=AAV_RSV_GFP:1656-1806	99	rAAV_RSV_GFP	1657	60	150M	=	1728	221	AACGAGAAGCGCGATAACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAGATGCGGCCGCAGTTACGCTAGGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATA	AB?>?@?C>BDCACBCBCEGGED?ACEFDDGDHEEEEHFDFDEEEBACDCEECGHIFEB>CBCFCHIFCDCC@@GDDEFBIEI?<?@BII??FG@E>1,2828>?:IID?5:??9=A5;49E=68731BH=;=8?=A:42=?CA@5,/=A	NM:i:1	AS:i:145	XS:i:0
virus|35|0|1-150=AAV_RSV_GFP:1727-1877	147	rAAV_RSV_GFP	1728	60	150M	=	1657	-221	GCTGTACAAGTAAGATGCGGCCGCAGTTACGCTAGGGATAACAAGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTG	68/B=24.-6*.>667*)(425/:?6E=45=@C??@@><IC?AF98A==1<?<F@B;BEA>;<<599><:BCIB<?;7D??EADG@FDBACBDDEDCECDDDEFEEGFGGHEECBBAB@?FGBDDDBBEHGCCDDCBFDBBC?<>>>:<B	NM:i:1	AS:i:145	XS:i:0
virus|36|0|1-150=AAV_RSV_GFP:580-730	99	rAAV_RSV_GFP	581	60	150M	=	799	368	TAACGATGAGTTAGCAACATGCCTTACCAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGAGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTAAATTCCGCATTGCAG	HE?BHC=ADBB@CA?BCECDGFHEGFHD@BBCBDDDGIEFEGIHFFEE@ABFEEFED?=@>CCBEFC@??AB===>=DA?@CIGDCC>D:754@<FA?A@41A:6;<=3173@49:2>:<?;;--764507.23(/,6A@DIB;35;=71	NM:i:2	AS:i:140	XS:i:0
virus|36|0|1-150=AAV_RSV_GFP:798-948	147	rAAV_RSV_GFP	799	60	150M	=	581	-368	ACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTAAAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGAT	CD:6084=359748C?=@>=.0561):=8DD=09>G@B?EICC@;BC>GEFG?:E;?CGIIH@@DGIIEHDDG<7CCCD?FABABCCEFDEB>CBCB?DBEEEFDDEEEEFEEHEDCEEEEDDEEGDEGEGFGEACB<9?AACDB;@???	NM:i:1	AS:i:145	XS:i:0
virus|37|0|1-150=AAV_RSV_GFP:1144-1294	99	rAAV_RSV_GFP	1145	60	150M	=	1340	345	AGCTGACCCTGAAGTTCATCTGCACCGCCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACACCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCAAAG	::>>@BCCAAA@>@ACDB@CDDBDEGGECDDDCDDEDDBCHHDFEEGGFFEHDBBACIFGICF?BBCEEEIFD;;@@@AEC>BCEHIHHIIBIII<7<>@657<3<EF>9:;0;0703BF@8A77:8:/,9840.8<4:8078892)452	NM:i:3	AS:i:136	XS:i:0
virus|37|0|1-150=AAV_RSV_GFP:1339-1489	147	rAAV_RSV_GFP	1340	60	150M	=	1145	-345	ACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACA	<D708677>5>B?D:9FGG@:85656CIIH=C>AA;9B8IFFD;=;:=A>E@BDIDD=;AA@@@:B@AEB>@@CEB@?DF?DBA?DEGIGIDGECGHEDCFHEEEEEEDCBFDDGGEDDCFEEFGDCDCEEDDC><BGDFCEFFABDA;?	NM:i:0	AS:i:150	XS:i:0
virus|38|0|1-150=AAV_RSV_GFP:969-1119	83	rAAV_RSV_GFP	970	60	150M	=	715	-405	ACATCCACTTTGCCTTTCTCTCCACAGGTGCAGCTGCTGCAGCAGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAG	B7?C?A51;;74';6D@9EI9><IIGE960+:H@EICI?A@:94;7@D?IIF>HCFAC?6@?A:@CIG<=B@CE@AAEEGABBB>:@C<B@BEBADEFHFHFEFIGDDDHEEDFEEFEEEEEEEEEFBCFGCDCBCCCDEBCCCEFA@DB	NM:i:1	AS:i:145	XS:i:0
virus|38|0|1-150=AAV_RSV_GFP:714-864	163	rAAV_RSV_GFP	715	60	150M	=	970	405	AACTTCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGG	>A==?@B@A?AABHCIDDDDB@BEGIEGEDFIGIGEBCDDCDBCDEHFBBFDGHGFGFCA@B@BCIFC<;?IACBIEIHIEAHBBC>@C7<@@BGCID>=8DI?I77<:A<@EAA?::;0-7;<;99F=7?5;5+:?:;;@G38,-9*93	NM:i:1	AS:i:147	XS:i:0
virus|39|0|1-150=AAV_RSV_GFP:981-1131	99	rAAV_RSV_GFP	982	60	150M	=	1107	275	CCTTTCTCTCCACAGGTGCAGCTGCTGCAGCGGGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGAT	?C@CC?@=?=<@>DIFBDDEBCFFDDDDIIEEEECBDBCDCDDDFFDCDBEEBEEFEDDDFIFEECCCAA?DAD<?GIFICBBFA:=<>>AECID7HIDE;;609DHIH<6<?DDH;;;7FC</C?>D@>33'/9A0)!"9<//4E@=8?	NM:i:0	AS:i:150	XS:i:0
virus|39|0|1-150=AAV_RSV_GFP:1106-1256	147	rAAV_RSV_GFP	1107	60	150M	=	982	-275	CGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCGGTGCTTCAGCCGCTACCCCGACCACAT	527B@;56G=A4;BD>BCE7:11:9C;C@C>@8B<<;BF>GADIIDBB>>;DG<8<@9<=HI=IIG13?9<A@B>>DFIGEIFECFCBAECCBBEEDDFEEGIEEDDCCCBDDCFEEEFFFIEFEDCBBDCD@@@BDDICA=BDEIA>><	NM:i:1	AS:i:145	XS:i:0
host|00|0|1-150=chrX:7729061-7729211	77	*	0	0	*	*	0	0	GGACTGATATGGGGATGTGAGAGTACCTAAGAGGTTCAGCGATATGGGTGGCAGATTAGGGGAGCCCTGGAAGGGTTAATCTAGAGGTGGAGGTGTAGTAAAGACAGGGCTTGGGAAGGGAGCTGGTAGAGAAGCTGCTCCTATTGGGCC	<??>=<>?@DCC@>BCGFGDDDCDBBCDEIHGEEEFECBABAEHDDCFEDEEFEGFD??BCEFFAB>BAHCC;;;;>AEC>AICEDHAE>??I65;IE;@?AACB=>DC:=CDD>=:>>F730588137909:=IE=520?@<04;=GII	AS:i:0	XS:i:0
host|00|0|1-150=chrX:7729356-7729506	141	*	0	0	*	*	0	0	TTCTCTGGTCTTTGCCTTTCAATCTGCAGGATTTCTTTCTGGTATTCCTACAACTGCTCCCATATTCCTCTACGTTGATTTTAACCCTGGCAGATCCCCCATCCTGCAGTCAGATGTAGGCACCTTCCAGGTGTCATTCTCCTTCCAAAG	==><?@??BDC?B@BACIHEFFEIHEGCDDDCDEDEEGGFEEEEEBDEACCCDCEFEDDDCEDABEGGAB?ACDC<=;BG<37<>=B=?<BD@GF=I?I@AD:24,:<E=C?655?CB<B?>?8377@2:>6CH</9I:<7<8476;7;<	AS:i:0	XS:i:0
host|01|0|1-150=chr1:22748956-22749106	77	*	0	0	*	*	0	0	AATATTTAGCTTTCTCAACAGTACACGTGCGTGTTTTGAAGCCTTCCATGAGAATTTTGTTGTATCGAACTAAGTCAAATGTGAACTAAACGCAAACAAGCCCTTAGGAAGACCACACATGCAGGTTTACAAGAACTTTTTTTTTTTTTG	;7<>CC@BCC?=>@AC@D>@GIGFCEEEGGIGECDECBCDEECDDFEEHDGDD?BCBAG@BCCCC?>@:>@CFGGFIFDCEDABIA@@DD>H?@=IG?2<BA=>;@A?;9AGCA=H=EE<8EC536<;8159;8@87:05>;?8036.3D	AS:i:0	XS:i:0
host|01|0|1-150=chr1:22748685-22748835	141	*	0	0	*	*	0	0	TCATGACAAGAATACCACACAGCAAAGGCGGGCTAAGGTGCTCATCTCTCTAGCGTTCTTTTCTTTAGGATCAAATGCATATGGTTTTGTCAACTTGGACATAGATGCATAGGAGTGTAGCACTTAAAACACAGCTAAAAGGTGGAACCT	99=?:@@C??BBCBDIGFHGFEEEDFEABCDDDFDDEFEFEAEEGFCCECDDBCBBECAACBC>?HEEEBIE@@???>@AEC<AABA?B:8=B=:;8@@F=>:=@?D?B@>:?G2?>;DC;HH@>I?FFIB?H7962689:;17969@<F	AS:i:0	XS:i:0
host|02|0|1-150=chr4:107475626-107475776	77	*	0	0	*	*	0	0	GTTCCAAGAGAGCTGAGCTGCACAGCATCCCAGACCCAGCAAAGCGCCAACACATGGAGGCAGATGGGTTTGCAGTGTTTGTAAATCAGAGGTTTGGCTTGATTTCAGGCTAGCCCAACCTATGGAGAGCTGCAGACCCCAGGGGACAGC	;=@==@DFGABEDFEFEEEEDDDBA@AADCCGHADCGGEDBCBEBD@DDCEEEDEDCC@ACCDBBCCBCIIFDBDIDBEBB>GIEA=?B=GAC7GCAD8:;HEHCD?:?64649A4:;?<97==E>BFI@=>H4?IICGB?<;<IAIIEH	AS:i:0	XS:i:0
host|02|0|1-150=chr4:107475479-107475629	141	*	0	0	*	*	0	0	GAAGAACTGTCAGCCCTGCTGTGATGTGACTTGTACCAGTTATGTCTTGCAGTGTGATGTGATGTTCTTCCATAGTGGTCCCCATCTGGGTCCCAATGGCTGCCTCTGATCTTCAGCCTTTTCAGCTCAGAGGTTAGGAGAGAGCAGGCT	9;?A=?B=AB@@@@>=@DCDFEFFEEEFGFGGECACFEEEEFCDDDDDDCFEFEFED@FHFFEFAFECDD@?I@;>8>>@=:>=@=6=>?<>B6?>7.2=:>9DAAC><FDGF?G=3=B:<;IF;:<2465469B@>B1,?A=C9:;9@I	AS:i:0	XS:i:0
host|03|0|1-150=chr4:14696464-14696614	77	*	0	0	*	*	0	0	AGAATGAAACCCTCATATGAATATTTTTTGGGCAAAGGATCACTCAAGAATTTGGAAAGAACTTTACAAGAATATTTAAGTTACTTGATCAAATATGGCCCAAGTTTTACTAGAAACTAGACGTGATTAATTCTGTTACTGTTTCAAAGA	;;8<CCB?CBA@ABCDEACBDDFFDFEDCDDGIIEHEEEEGGCDDDHFEDCFCACCDBDEEDEABCDBE?AEAB:?@AAC=BEFDAC?CFAIA@?979=9:6@5A?E@>:<9877<A<5F@EAABEE@E<;@=85<<7458BBI?AFCCC	AS:i:0	XS:i:0
host|03|0|1-150=chr4:14696570-14696720	141	*	0	0	*	*	0	0	TCACATGACAATTTACTCAATTTGGTTTTCTCAAAACATTGTAATTAACTGAGTTGTTAGCCCTCAGAAGTACGGCTTTTCATTTTAATTGCTATTTCAGCAAGAATCTTTGAAACAGTAACAGAATTAATCACGTCTAGTTTCTAGTAA	B?AA@?@??AA@??A>CEBCD@AFDCGFECHDBCHBCDCFEGGE@@BCDACCADBCDDDHGIFDDAH??A>B?BA=DB><HH?9@FBID<@@;2E?<<>CBA?I>?@?C;:==<;F=;?BA9>D<BED=>D@:4>4<9081:;:3?:@6/	AS:i:0	XS:i:0
host|04|0|1-150=chr1:64467655-64467805	77	*	0	0	*	*	0	0	CAACTTCTAGGCTCCTGAACTGATGGGAATTGATTAGTTCACTTTTAACTGCAGTGTTTAGTAATGTAAAATATATTACTTTCATTGTAGTGTTCTATAACGAGTAATGTTCATTATAGTATTAATTGGAACATTCAGTTTTGTGCAATA	9<A>???A?=>;><@BCDDCDCDEIHHFDCHDDDEEEFEDCCEEDFFFEBCDFEECDDE@BF>ADCD>;<DFAEIDA<<6<=BDB<46?BIGCA>EICE@9@CDAH<CH?;5ABD=I=,4?CHII?B<;<=:?8C?IGB@<5;=>::;A4	AS:i:0	XS:i:0
host|04|0|1-150=chr1:64467893-64468043	141	*	0	0	*	*	0	0	TTTCAATCTGGGTACATATATCATAAGTTAAGATTGCCTATCACTTCCAACTTGATATGGGCAAAATTAAACACAAGCAATACCGCATCTCAGGGAGTGTGCAATGCCAGTCTCTGCCAAAAGAATATAAGATGCTCTATCACTTTATGA	?@?<;><AABEABAAEECCEDECDDBBCCDCDBBHGHFEBDDDDEEEDCDEGFEEEBC>AABGICGEDGIIIG>CD@GG=C@BE@?IEB@C?@G@;:8?=E8@IA;B>8;559:>:84IEIE:4=3<23<<>6A>-40).3303',7893	AS:i:0	XS:i:0
host|05|0|1-150=chr7:63677746-63677896	77	*	0	0	*	*	0	0	GTCAAAATGTAAGGGACATTATACATTTTATGGTTTTAAAAGTAAAAACCCACACGGGCAAGGAATGGTTTCTTTGGTTCCTCGGAAGTCTAGCTGGAATTAGAAGAAAAAAGGTACACATATCCTCCTGGGAGTTGGCCACAGGAAGAT	?DA@BA?=>B?CFEDDEDFHGGHFEDBCDBCFDCDC@BCDEEDDCCCDDEDFEEDCBCDF???FCGFDC>AA??@AAC?=:F;9<A>?CA94,.8;GCB818@8@DA>@7;E:1/=>;:;7==>@69;;8989<HD75<9=<C@5/(9/C	AS:i:0	XS:i:0
host|05|0|1-150=chr7:63677564-63677714	141	*	0	0	*	*	0	0	TGCCAAAAAGAAAAAGAAGACAGAAAGAACTGAATAGTGTTGAGTGTTCCTGGAGGCTGGCAGAGCCTGGGTCATTTGATGCTGGTTACAGGTCTCCTGTCACTACAGTGATGACAACCACAAGACAAGCAACTGCAGACAAATCTAGAA	>>>?<>>==?EGBDHCDDBBBBEAEFFECDDDCBDFCEDEEHECDEDEGFFCDEGGFEDFBCIIAEDC>BEDACG<BC?CBAA:@=;.:<>>B?@D<<A?DH:9<IIC:BIF??C@B?>7HBFD?EAAGGB><9=>BB@87FF438323D	AS:i:0	XS:i:0
host|06|0|1-150=chr7:35449032-35449182	77	*	0	0	*	*	0	0	CTGTCTAGGCTCCAGCTCGACAGAAGCAGGACCTACCTGAGTCGGTGATACTCTCTCAGCTGACCACCACCCAGCCCGCAATTGTAGGGCCAAGCTCCAGGAAGGGCCATGTCTTGTGCCTAGGCGTGGAGGAAGCCAGAAGGTACAGAG	FEA@B@EDDDAB@HIIGECCBBDDD@DDEDFDEDCEEECBGFGFEEEEGFBCDCDDCC@?EDCABBFEFBDCCF@AFDAI@@F@?DCFIIEECAEEEGI74>=FCF<;;=B@?>35069B:5$09;AA>?I64?GFABGCAG17>E:559	AS:i:0	XS:i:0
host|06|0|1-150=chr7:35448922-35449072	141	*	0	0	*	*	0	0	AACTTATCTGATTCTCCTATTTTGTGTCTGGCGGTGTCTATCTGGGCTTTGTTGCCTAGAAGGAAAAAAAAAACGGGGGAGGTGGGTGTTTAATAATTACCTCTGTTGACCTCTGTACCTTCTGGCTTCCTCCACGCCTAGGCACAAGAC	?@A@@BAEA??BAFCEEEEEIFEFHDDDABEGFEFDFEEEEEDDDGCCCGBCFDDEDIIGCCC=@=ACCIFD>=EIE=;<C>DBAECEEGEIDGC==5?9D8?EF?=6D@<@<D<A99;:#:359E;C8>A9?A>843-=:468=@@H<>	AS:i:0	XS:i:0
host|07|0|1-150=chr4:84426118-84426268	77	*	0	0	*	*	0	0	TATTTCAGAATTATTGGAATTTTTCCTTCCCTTGTGTGAAAGTTAAGAGGACTGTGCATATGTCGATAGGTAATCTACTTGGATGTGAAAACATCTATTATTTTATTCAGTGTTACTCTTGAAAAAAGAAAAATAAGTGATTTCTTCTTG	?@???@@:;?ABBIEDDDDCDEEGEEEEDBEEFEFEFEEEFFFECDBCDDFFEEECCDEFFFEDAD@>C:9?B@9;:?<@;:;=?@AACB=7@9799E@AHHDCF>519;974>:<H<?:;><7)+@>ICBI:EHB@7:*06>;8A9-:;	AS:i:0	XS:i:0
host|07|0|1-150=chr4:84425850-84426000	141	*	0	0	*	*	0	0	AAATAGCGAGCAGCCTTTAGAAAATTTCATTAAACATTCATACTAGGTGATAGTGGTTTTAGATTAATTTTTAAAAAGTAAGCCTATCTACGTGAATAGTTGCATTTACTAAGTTCATGGCTACAATGAAAAGCACGGAAAAATGTTCCA	????@@CBHGIEABADDEFCGGIIHHEEEGEC@ABBB@@ADDCCDGFACDBBCBCCCD=<?AC=CE?AGBFD88BFCIFI:>@IFEHII=IA4:<B=?IFB-22B>>5??>DC5=;@FB:=<@<=A9:FGDHF;8;;4=<;40<;<979C	AS:i:0	XS:i:0
host|08|0|1-150=chr6:99156362-99156512	77	*	0	0	*	*	0	0	TTCAGGGTAAAATATGCTTTTTTTTCCAGAAATAGACACCAGAGGAATTAAGAAAAAGGGGGTGGGGGCTGTTTTTGTTGTGGCTGGCTTCTCTTTACTTACCAGAATTATCTAAACTCCCATTTCTGCTTTCTTTCAATTTGTTTGCAT	:9>A?@@A=>ECFIFIHIGIGDDFGFGFFDCDABADFCDCFEFGEEEADBDDEEEDFIIECFC@BACIHA9?GFCGBBFDHIHBAFCE>?@@@@CA7=7:<=B;ADA><<68=CBC8?FCC>=;IG??A>0*1,;?76:63:C0,1=A7:	AS:i:0	XS:i:0
host|08|0|1-150=chr6:99156633-99156783	141	*	0	0	*	*	0	0	GATGAAGTCTCAGCATTCTAGAAGGGAACTTTCTAGAAAGGTGGTGTAGAGAAAAGTGTTACTAATATAGAGTGCCCTACTGGGACCCAGTACAAGTTGGAAGCTAACATGTTCTTTAGCAGCTAGGTAAAAAATTAAATTCTAATTTTG	B@????CA?CCCBBBACCGDDCDDDFFCAEFDCADABBCDDEDDDECGCABCCAAEDCBGIFHFGGFDGBBGHDAAEHIFBHDICB@/8B=H3469@66F@ADIDA@?CI9FGFFGDEICGID71D@<958:79?(0(0)46<B;89532	AS:i:0	XS:i:0
host|09|0|1-150=chr14:114917441-114917591	77	*	0	0	*	*	0	0	GAACACAGAAATAGTACAGTGAGAGCTAACGACCACTATGCAAAATGGTCAGCCTTAACTATTGAGTGAGTACGTCTTTTCCTTAGGATATACTCAGAATTACAAGAGGCAATTGTGCTAAGCCATAAACAGTTACACCAAACCCCAAAG	?D@@B@<<@9<@ADDEGDDDBBEFGDEDABCABCEEFFCFEEEFGFFEDCEIHFEDGEIHFGDDDDGD>HCFGBDCIHAGB=?8IFGEC?DDD@GGCB@;GBD>>:BGIDA93066:;=CC>BI<?H;C;=A=:==51;5<5686G9;A6	AS:i:0	XS:i:0
host|09|0|1-150=chr14:114917314-114917464	141	*	0	0	*	*	0	0	TCTGATTTGTGAATTCCCTTGTCCATCAGATTCTAGAAAGAGCAATACTTGGCAAAGACGGAAGCTGACATCTAGGTAAGCTGGAAAGGCCTGTTGCTCAGAATCCCAAAGCTTGAGCTGAAACGGGCTTTGGGGTTTGGTGAAACTGTT	8AB?>B;A@AFBBCCFC@@CCDAEGFFEEBBACCDDDDGIGFEDGFGEFIFDDDFEE>:?@CDFDIECCEBBIID@CB:=CIF@D?CA@@A9:EDGIID:<;AE=7:,,8B??G;?:7?E<C>>56694=<<I@:<;D>/31>;1-&014	AS:i:0	XS:i:0
host|10|0|1-150=chr19:10646891-10647041	77	*	0	0	*	*	0	0	CCTCTCCATCCTGCATGCACAAATAGGAGTTGCAGTGGTGGGCAGAACCTAGACACTCCCACAAGGCAGCCTGGGGACTTCACACCTACCTGGCACAAGCACTGAGAACATCCGGGCTGGTTCCAGCGGCTGCCTGATTCATACGTGGCT	@A@@A;?@@?DDFCCDDBBDDCCEGHFDDDECCGFGFDFGHFGHDDFEBBEFGFFDFDBBBDEIGIFHGEA?B@AAEDDC58>?FI>?<8II<IDGFAF@:D82:7:<A:89>>B54319:6?=96:519@<<699?EA@5<@/295>;-	AS:i:0	XS:i:0
host|10|0|1-150=chr19:10646681-10646831	141	*	0	0	*	*	0	0	AGTTAGAACAATCAGTCAGCCATCTACCACCCAGGTCCTACCCACATTCTTTCCCACACAGTTGATATCCACTCCAGTGCCAAGTTCCTCCCCACTGGGGACCCTTGGACCCCCCTCACTCCTCCAGGGGGCTGTAGGGACCCCTTCCTT	?>>@B@C=GABBDE>BGDEEDDDBECFEDFEFEEDCIIFFDBBCFFEE?CCEFEEFE@EDDBGDBDCGCAI@FE>7>AIF=6?@B8;:3A>=@A=DGA?EIF@H;@B;8;BII<E;G?>;>F69;4>9EEI6F<;GIC<;?35421<@EG	AS:i:0	XS:i:0
host|11|0|1-150=chr18:84376768-84376918	77	*	0	0	*	*	0	0	TCAGCATCATTTGGAATTTTTTCTGAGTTATTTGGCACGTGCAAGTTGTTAGAAACACCAGAACATTCTGAACAAAGTTCAGAGTAGCCTTACGTAGGAGGGAGCCCCTCGGCTGTCACAGGCAGAGAAGGACTCTGGCAGAGGTCAGTA	<ABA=BABCBGDDFGGGGFEEECFEEFCGIGHFGFEGFECC@BEEBABBCCEFBEEDC?ABEDDBBAEGIB>8:C>@@B<AF@CCBA>?>DID96@<7;<<::@8<7;=B=69CE@B@AI9>DIB4208A8.9:>4<;048:;<B@;:=;	AS:i:0	XS:i:0
host|11|0|1-150=chr18:84376666-84376816	141	*	0	0	*	*	0	0	GTGCAGACAGCTGTCAATTCTGCTGAAACATGCCCCTAAAGGGCAGGTGAAGATGACCATGCGACACAGCCATGTGGCTGCAACCTGTTTCGCGTGCATGCATACTGACCTCTGCCAGAGTCCTTCTCTGCCTGTGACAGCCGAGGGGCT	A=;:?>@>DCCABE?A@>@ACDEFEEEHGFEEDGHIGHFEGHFHGEEEEEDDDBC??BC@BDD?BC@A=>>EC=?<<>C@AI@:DE9AB=@>4CCD;4:;HC<:6/9<?F949><=<7<52%*(&*66)<B6=/92<=>>6-/+.4<6I=	AS:i:0	XS:i:0
host|12|0|1-150=chrX:55638399-55638549	77	*	0	0	*	*	0	0	AGATTTTTCCCTAAAAGATTATCAGAGAGGTCTCCTTTTATGTTTTAGCTTATCAAAATTTAATAAGTTCTAACGCATATATATCTTATTGTAGAAAATTGACTGTATTTCAAAGTATTTTTCATTATTTTCTACCAAATATAAGGTCTA	8;<:>?BBDBBD?<<>ACEEGFHHEEEDEDDEEIEFFCCFFEFEDGFCABACCCDDDC>GEDDDIIEGEDHCFFBFGIFHDDB?>=A@DIG<=>D;=19-867<=;7@IB;8>;<@:8A;;2<@BB?9:B29C?))(7>>F;*65?-7.E	AS:i:0	XS:i:0
host|12|0|1-150=chrX:55638110-55638260	141	*	0	0	*	*	0	0	CAAGGAAACTAAATCTAATTCTTAGAAATCTGTGAAAATTATTATTTCCTACTTTAAGTAGCTGTTAATGAGATTTTAAAATTTGATTTACATTCATTGTGGAAACACAGAAATCTTGCACAAAATCTTAGAAATGTAATTTCTGATTTA	:A@>AA<BA>BBB>=@?CCECDDCEHEEFBFECDDDEEDEGFBAEDAACDADA?DDD;BA>C?><:?A<IFBB:@A>AAB@BFIIA@IIC?9,35;3E=:?B?;8<AC:49:28?BG=27@@>B758A:+6FAADFEG9B87;H@7;5;G	AS:i:0	XS:i:0
host|13|0|1-150=chr16:88636621-88636771	77	*	0	0	*	*	0	0	TAACAAGCTGAGCCATCACCTTAGCTTTCCAGTTTCTGTTTTATTGTTGGCCTTGTTTTTTTTTAGCACCAGGGCTTTTCCTTAGATGCCACATATGTTCCACAAGTACTTGATCACCGGGCTACGTCTATTTCCCTAGCTTTTATATCT	?=<@=EDAHED@=@AEEFFFDEDDBCDEFHGHEGDDBCBCDDDBCAEFEDDGGIHFEEFCC@GEDGCAC<B>BGEFDA;<9=@<D?>?::==::;C;??496;C:6C528IGIG=9.57E<5676134/C79=<G89H38</:8>AA;D0	AS:i:0	XS:i:0
host|13|0|1-150=chr16:88636879-88637029	141	*	0	0	*	*	0	0	GACACATCCTCATTTTAAACTGCCTTGTTGGATCCGATTAAAACTGTGCGAGTGAACCACTAACTGATAGGTACACTTATGCGCATCATTGCTCTTCATCAAGTCTTTATGTCGTTTTTCAAAACAACATATGGCACAACAATAAGGAAT	BDA@CCDB?;AC@BBGFBEGGFCFCEEEDCDIGFDDEEEFHFDDCBCEEGIFEEEBFDFDEDA>;CGDBCBA>>>;>CBBGC;CHI@BCD6;?<AGIIGEDA9FHB;0998@B85DB@A54=;;;>78A><98<8A?B4752/78:B;C9	AS:i:0	XS:i:0
host|14|0|1-150=chr2:133795179-133795329	77	*	0	0	*	*	0	0	GATAGATTTCCAGAATAGGTTTAAAGAAGGAGTATATTCTAAGGCCAGTACTAATGCTACCAGATTATAAAATTGCCCAGAATATGTAACACATTCAAAACTCCATGACAGGTGGGTATTGGGGGTTGGGGACGGGGACAGATCAAACAG	>=>=AE?>>ACDFD?CACCHFFEEDDDDDAADBCCEDACDD@ABCA@ACDDBDECCCBGFA>ABABHFGE?EDC=<CAAB>>B::0-:>?79B;9>8<?===?>B??;<<611446:<=8958611<<-0,,/<8:9387CCA?2=:638	AS:i:0	XS:i:0
host|14|0|1-150=chr2:133795445-133795595	141	*	0	0	*	*	0	0	ACCCCTATGAGCAATAGCAGTAACTATTTCAGCCCTCCAGATTAAGGAAAAGGCAAGTCACTGGGATGGCTAAAGTGGAATGTCCACGGGAGCATTACAGTCATGGAGACTGAAAATTTGAGGGACGTGTAGTGGGAGACTTTAGATGTT	FBG@@A<>=<<<8@GIGEEEIGFEEHGFBBADCCCEEECDBCFFGFEECEEEHCD?@DEEFBCCCGEDEGEE@DEFIBD@CDB@=<:=B:8=D6B553;=CCC;;A3:<H>5:;97*4@@GB;:B:;;;9;;;;IEG=@@B><68D>>.1	AS:i:0	XS:i:0
host|15|0|1-150=chr2:96164703-96164853	77	*	0	0	*	*	0	0	TTATTGTGGCTGCTAAGAAATGATGCCTTTTTTTTTTCAAAATGATTACATTTCTCTTGAAAGAATTTTCGACTCGGAAATGGGATAAAGAAAGTAGTTTAGGCTCCCTCAATCCTTGTAAGTGTACAGATGTGATACACTGCTAATTCT	AB<:?=AB=?B=>CDFFCC@BBAAEHFEEDDBBDEDEDDDGGGFEEEGEDCACGDDFEC??D>AD?ABB@AICIFDC=DB8=<:@HE:BH@A99;DHFIIII=IF;:64;774<B<;A?IA;8DI584064A<C>GD8EGIG@AF@/-26	AS:i:0	XS:i:0
host|15|0|1-150=chr2:96164565-96164715	141	*	0	0	*	*	0	0	GGTGTTCTAGATAAGAAATGATTTGTTTCATGTTTCTGACCCCTCCCCCCCACTCTCACTCTCTCACTCTCTTCCCCCACTTGGCTCCCAAAACTTTGAAACAATGTATTTAATGCTTTGATGCTGTGTCCTAAGGAGAGAATTAGCAGT	:9<A@:>?@?C@A>@BCDDDEEB@BHDFEEEHCBCHGGGE@DBCGFECEEEECCDCCCC@ACDDEDCEF:;?@>AE>>A:==@@DEC?C>D?I=G>FCAII789A7::926CIDA?>59373@AC;;:;AD@5=?896=<ACCA4>5/45	AS:i:0	XS:i:0
host|16|0|1-150=chrY:35713645-35713795	77	*	0	0	*	*	0	0	CCAGGTAAACTGTCAGTGAGAATGGTGAGACATCTATTCTTCGCAAGGTTACCATAATTAAGGGCAGGGAAAATGAAGATAATGCAGGCTTATTGATATATTCCCAAAGTCTAGATTTAATTGACACTCTAATGACTTGCACAAGAAACA	@@@@AAEBEBCBBCCCDCDFEEFEEFEEEEDDDBACCDDDFDF?BDEEEEFDDDFCA@??<;CIHEFFIDBCIEEIEHEE@BIAA:@CIFID?B;9515::?7;?>;D49;6:DEGHIB;=:5@?A:7BAE=I@ADB@6=5<E58,4>;:	AS:i:0	XS:i:0
host|16|0|1-150=chrY:35713476-35713626	141	*	0	0	*	*	0	0	CACTCTTACACCATCTCCTGCCTTCACAACATACTTATTAAGATGGTGAAATATTGGGAAAGCCATGGTAGAACAAATTCTTTAAAGCCCAGTGCTGTTCTCAACAGTCATCTCCTGATTTTCTATATGAACCTCATATGTTACACTTTG	CABA@CC@=<@?=?B?BD?@FEEGHHHDEDBDEIFEEFHIHHEFFFEFFEEFDDDHCFE?>A@BD@DADAAHIAGHFEECGHCBFGEG@CA@@>?9368:A@I@=9>.<FGC>DEAEA@7<:54=>;?C=C=1=154/;>BI@<;;>97:	AS:i:0	XS:i:0
host|17|0|1-150=chrX:12388619-12388769	77	*	0	0	*	*	0	0	AACCTGCACCTCAGCCAACTTGCCTTTGCCCCAACCTGGAAACGTATCCCTGCTCACTTCCTGCCCTCGCAGGATCCAATGCTTCTGGCAGACGTAAGGCAACAAGAAAATGACTTGATGTATGGAGAAGCAGGATGATTGCCTCTGGCC	@=@=>@CB?CHDFFEFECGFEEDDDBDCBCCCFFCDDECEEEBEEDGEEEEEDEEGBBCCACCCCCGDCGHIHI=CC=>B@D?FIB<>AC9=G777@C8CA=AC:9<D?IA?>;DF8=?6?I.3>?>9>DDG.=6;<C<:86:6=67(.6	AS:i:0	XS:i:0
host|17|0|1-150=chrX:12388543-12388693	141	*	0	0	*	*	0	0	TTTGCACTTAAATCTTAAGGTCTGCCTTCTAGGAGAACCCATGTCTGTCCTTGTATAAACTCAGACAATAGACGAAGGCCAGAGGCAATCATCCTGCTTCTCCATACATCAAGTCATTTTCTTGTTGCCTTACGCCTGCCAGAAGCATTG	E>>?AACB@BC?CHIIEEDBCDFEDCDDDDEEEEEEADGIGFGDDIIGDECCFFFGCEEGFECCBEDCEIIEDCFD?;>@A=5CIG>:<CEB=?9@BHB@9996IC;:;<@?>554=FF?@FIIIA<:/66B>47;@?46@-):::=::8	AS:i:0	XS:i:0
host|18|0|1-150=chr14:69347275-69347425	77	*	0	0	*	*	0	0	AAGAACGTGAGCCAGGCTCTCAGCATCTCTTGACTGAGGCCTGTATCTCTGGCAGCATGAAATTGGTGTGCACGAATGCTGCTCTAACCCAGTAGTCCCCACATAGGATCACAGTCAGGTGGTGTATGGCTGCGTTTGAGCATCTACTTG	@>@@>?@@FFEIEIF?BDBBCBCDED@BEFDDDBCGDCDDAEEHFEEHCBDEBDCEEDCHDEDEGFDDIHGIFBE>87;<9<?FI@8AB@B<-39ACE8;GE9=;:5557?EIEG@>D=;<<B>@5:3>25-.(>B=G=CE@C:E?:93-	AS:i:0	XS:i:0
host|18|0|1-150=chr14:69347418-69347568	141	*	0	0	*	*	0	0	GTCCAGGCGATATGGTGGGAGTCAGTCCTCGTCATCCACAATGACAGAATCAGAGCTGGGGCCTTCATGGAGTCAGGGCCACCCTGGTTCAGTGTGAAAAAAATCAAGAAGAGGACAATCAAATAAAATATCTATGGCCTTATCAAGTAG	9>:<@>?@A>>?DA?B?CDBACCDDDDDBCDDBDFDDEGGEBACDEFEBBCFEDDEABDDDIHED@ADHIGHE=?@??>>=9I>?:=4>C8<<HG?F;=::?DA@1=AC?8?B@,08?H>7:91<;3;6@I>I;BE?8C=</BC@9:E98	AS:i:0	XS:i:0
host|19|0|1-150=chr13:36409130-36409280	77	*	0	0	*	*	0	0	ACAACTTATTGATATTTCCAGTTCAATATTCACTTATGTATGAGTCTTTAGTTCTTCAGAGAGTGTGCCATTTAAAAATTTTATATTTCTTCTAATCAATGACTCACTAAAGGGCTTATAGTAATACCAGATTTAGCATAAGTCAATTTA	@?9=>???@ADCEDDFFCDDDDCDCEGEDDCDEDDDDDFFDBCFEDBCFEDCEBCDDGEADD?A<DAEB>A=AD<;=;>@HII?E@C<=CDBBIEEA>IHEIIC=GCGDC@BA;;=EAI.@;=5@56@?830<997;5?57A8==36D?A	AS:i:0	XS:i:0
host|19|0|1-150=chr13:36409500-36409650	141	*	0	0	*	*	0	0	TCTTTGTTATAATTTCTAATTATTAATATCAGGGACTGGGATGATAAGCCATCATTTAGAGATTATTTTTTAATTATGCTTTAAAGATGAGATAAACACAAATGTATCACGTCCCCCATTTTATTCCACTTATTCTTGGTTTAATAATTC	AC@AACCDABFEAACEEEDCDDGGFGDEGFEHFFEEGEFGFHIHFGHFEGGHFEFEDEE@CABDCCFGF<>IHGDC>@@AABBCBA9ADECC65<79589759EFFDI74HC<BGHD6B<5.58?BGHD8=A>-89C<;>:;9?AG32<?	AS:i:0	XS:i:0
host|20|0|1-150=chr3:138052247-138052397	77	*	0	0	*	*	0	0	GTCCAGACACTTCACTCATACGGGTGCTTTCTCTTTTTGTGATTCCGAACTTATTTCTAACCACTTGCCCTTCAAGTTAGACAGCAGGTGTATTTCTAAGAAGTTGTGAAACCAAATTTTATTTTTTAAAGATTCCTATCATTATGAGAT	?<A>BCAACFDCC?ACCFEHFHGGHFEEFIGFDDDBCDDCCEGGHGGHD@FEDDFDDFHFFFECE@B?A>C>;>:@AB=8=BBAAA?@E?F>@C4GFCFB@B?DA>I?;BBD=@?7<<<II>8:=7?A7/56707>EEA10126=7100:	AS:i:0	XS:i:0
host|20|0|1-150=chr3:138052320-138052470	141	*	0	0	*	*	0	0	ATGCTGGATGCTGTCGGACGTGTGGTAAGGGATATATTTATTTTCCAAACTACCATTGTTGCACATTTTAAGAATCTCATAATGATAGGAATCTTTAAAAAATAAAATTTGGTTTCACAACTTCTTAGAAATACACCTGCTGTCTAACTT	?<>?@ADACEFGEIIHCFEBFGEBCDDACDCCDDCEDA?BCDCEGDFEFFGEEGIIFDD@C>;@A=>EID<A;?@A<C<IE@96=C>8=D>9FHE<<8:<DGEE;83=D@=::=8CF<<BB?9>8.,:35:'45.84CAC:583:4779:	AS:i:0	XS:i:0
host|21|0|1-150=chr6:4621568-4621718	77	*	0	0	*	*	0	0	CTTAACATACAGTTTGTCCATCTGTCACCTTAATCTGTGCCTTGGATACTGCAGAGTAAGAACCATAAGGACATAAAAACGCCCTGTTGTTGTTGTTGTTGTTTAAATCTTGAAGTGTCTTGAATAAAGGATTGAGAACATAAAGTGGTC	<@<>AA?@BBBAAD@<ACDEGFEEFEEEDFHFACFGCCGFBAC@CBCDFDCDCEFFECABC?@BDABBE<>CCGDCBB?9?@DFC@1;;<867=B58:6ADGCA59=EDD58;ADAABD@>=;<DDIGIE<743D;>8=AF;52/48=D9	AS:i:0	XS:i:0
host|21|0|1-150=chr6:4621362-4621512	141	*	0	0	*	*	0	0	GGCATCCTTTGTCTCATTGCAATTCTAATTACTCATTCCCTCTAAATGTAGAAATAATATTGCTTTCTGTCACCTAACTGATGCTAGACTAAGAAATTTCTTATACTCCAAAACTCCATGTAGCAGCAGCTGTGGTAGGAAAAATAGACG	>>=>AB:8<@EIHGDFBEFC@BDDEEEFEFEEGFBDDDDCFEBCCHFEFEBBCFEDEABCB?=>?ABAA>:@CD<@;8<AIA<>;IIFCG@>@7)699;E<ED>><HID>8:C:G246ABI=;<9:ADHB@9>@?5453)/>7965A>/2	AS:i:0	XS:i:0
host|22|0|1-150=chr1:161339159-161339309	77	*	0	0	*	*	0	0	TTCCATTAGCAAAGAGAAGCCATCCTTTCAAGAAAATGCCACTTCAATTCTAATCAGAAGGGTAGGGTCATGGCAGACAGAACAAGAGGAAGAGAGTCCCAGGATGCATATTCTCGCTAGTAGAAATAGACTCATAGTAAGTTGATACCA	<9?B?CBDDB>?BCADFCCDEHFGECDDDB@BBC@@CDFDDHFFFFFCBEFDDEBCCDFC=?EC@>=A@<:>>@CCC?<;>>>;?<?@GD62=3144=/7@ECG=5BA??AC@:<AA?B?=D=B?6+)%./1A>77HGIIFF;<CE?:EI	AS:i:0	XS:i:0
host|22|0|1-150=chr1:161339044-161339194	141	*	0	0	*	*	0	0	AACTTGATCCACTTTCATGCAATCACGGTTGTCTCTTACATGCAAATGTGACTTTTCAGTGCAACTTTTGCCTTGCTGTCTCCTTTAACTCTTAAGATCCTTCCTTATCTTAACCTGGTATCAACTTACTATGAGACTATTTCTACTAGC	>4?>??CBD=ABEHIGEHIGEEGFCDCDDCBDHFEDFFDBC>BADHEDDCEFBCBEDDBFGF@@;<;BE@>;EC96;8=DE=IFIEDF<>;>@;68347B???5@E:25==7C=<AEE>@BIBD>DB34E=:54'479589475:76845	AS:i:0	XS:i:0
host|23|0|1-150=chr15:55541344-55541494	77	*	0	0	*	*	0	0	TTAGAAGTTCCACAGTTGCCGTCTGAATCATTCGTGCAGACGTTACTTCTTAGGAAATAGTTAACACTGTTTCATAGGTAGTGTGTTTTTACACTATTACGGGTCCCTGTATTGTGCTCAACTTATTTGCTATGGCTTGAGGCTGGATGG	<8;;>?A>=6:?AA>ADDCDDDFDBCBCCDCBD@EEGHHECDFDDDCGHFEEEFDDCCCABBCB@DAC@A7AAEDIEC>DGDID?8;?@B@=BIFA>CICH<C>9@?>=IG<@CGG:9C@@I78<78:,5859=A@;DF@1<EE<47;@<	AS:i:0	XS:i:0
host|23|0|1-150=chr15:55541170-55541320	141	*	0	0	*	*	0	0	AAAATTTAAAATACTTTTAACAAACCTTATCGTATCAATATTAAAGTTGAATTGTATTGCTTCCTAGTGTACAGTTAACGTACCCACATGAAACAGAAACTAACTTCCCAGACTTTTAAAATACTTATAATTAAAATTCTGATTTGTTGC	?;=<:AGCEFIIFCCDEHFEEEDGHHFIIGGCDCFEEGFGEGEDBDCDDEEACDECCBBHEDDBACB>A@?A@EHCBBAADA>ED?<@?9<A?A@HBIDBB<;=I;?717<<=D;??>-602:8@?93-7A>B?=H7;/59C240EEGAE	AS:i:0	XS:i:0
host|24|0|1-150=chr6:77751216-77751366	77	*	0	0	*	*	0	0	ACTTGAGCCTCCCTGCAGGCTCAATTACTTCACCATCTACTCTCCTTTTTCAGCATCTAACCTTTGCCCTCTTCTCACCTCCTTTGTTTATGATGTGGACATGTGGGCATGTACAGTCATTTATATGCATGACGTGTAGACATGTAGACA	BAB@A?B@A?@B>>FHDCCDEFFCBAB@DDDE?@DBCDGFACDCDADGFFDFDDDDDEEA?@B?A?@=?A?EAA=?ACGD?@=@B=87;E?I>>E>=4<??@DB?CA81.6683869@2B.:;;<;B5-:?C@E76+?GA9ICIA5=/15	AS:i:0	XS:i:0
host|24|0|1-150=chr6:77751459-77751609	141	*	0	0	*	*	0	0	TCCCTTAAAATGCTGTCAACATGCTGATTTTTTCTGACACTACAGACCATGAACACTTTAGATTTTGCAAGAAAGGTGGCATCTATTAGATTTAAAATTGAATTTTCAGAAAGCAGCAAAGACAGGAGCAAAAATGATAGAATGTGGAGG	=B@B?DC>>@<;BIGEBDGFDDGEFFGFGFFCDB@CDHFFDDECDDDDBDDCDDEDDB>BGIFDA==@BBFEAAAA=<AFDIFA?DC>ACB@BIDB<69>>7C@G;EBGIA<;:;;39=@I=95=B>8.57<;?9:B73@201257@DC=	AS:i:0	XS:i:0
host|25|0|1-150=chr7:136832912-136833062	77	*	0	0	*	*	0	0	TAAAATTAGAGTCAACAGGGTAGTGATGAAGATGGATCCACAAGCAGGTGCTTACACAAGCTGCTAATAAGATATACAATAGGCACCATAGAAAGACCTAATAGCGGTAATAGTGATCTCCTCTGAGCCAGACTGGCACCAGAGTTTGAA	<=:9/8<9>>>?@GIGFEDEHECCDECEEEIGGFADDDDIGIGHIDCDCDBCEEEDDBFEEEDDEBBACGC>7=BC?:??BBBD<B<==?95@>;B>==?86@?:<DCDIIID=88:;E9/669<?IH>CB851038>6<;?AB07:6>?	AS:i:0	XS:i:0
host|25|0|1-150=chr7:136833258-136833408	141	*	0	0	*	*	0	0	TCAGCACAGGCTTCCACACCCTCCTCCTGTCCCTTGATTCTGGTTTGCAGAGTATGATGACCTCTTTCTCCCTCCCCTTCCTTTCCCCCACTTAGCCTCTGAAGGCTGAGTTCTGGGGTCTCTGTTTCCCTCCTGCCTTTCTGGCTGTGG	<=??;;=@C@CGCBECBC@DCHFFEGECBECDD@BCBCDGIHFEEEEHEFFECDFDDFDADEEEDCDCDADD=;>:=?@HFB>;@@=8@@II31;E8?GC?C?@7.;?658;C>;;9G>::?=AE<?>DCC>H@=:?BFBGDIBDI5,47	AS:i:0	XS:i:0
host|26|0|1-150=chr1:32153882-32154032	77	*	0	0	*	*	0	0	TGTGAAGTTGGAGTAAGTATGATGGAAACACATTATATAGAGTTTTCAAAGAGTTAATAAAAACACTAAGTAACAGCGTTGAGGCATTACCAACATTTTACAGAGTAGATAACCTGACACCCAAACAAATGAAAATGAGAAATTTGAGTT	?A@?;CD<@ACGA?=BAEEEGHFDDECFEECEEEEEDDFGFACBCECBEDCEDCEDCCEDEDFC?=BCFFE>>@>ECBDI<=?DBFCIC@:3>HDH8>BF>AC<9>@:6<9:<B=516*887;IAH7CA=>H@B@2047@7G:+258328	AS:i:0	XS:i:0
host|26|0|1-150=chr1:32153746-32153896	141	*	0	0	*	*	0	0	TTGGTCTTCAAATCCACCTTTAGAGACATATCTAAATATTGTAATAATCAATGACTTTTTCATTCTTGAAAACCAATCTCACTTTTAATGAAAGGAATATCTTATTATATTCCTCAACACATTTGCTTACAATTCTAACTCAAATTTCTC	?;A?=99>@>=>@?ACDDDF@CD?BBDDDDBCDCDCDEECEDADDEFEEFGGEFHFEEFCCHFCBBBCDFB<?=<A<:?<8=??=D;CDDAGFCHIIG8=27A945)4:>B>>=4@>=9;2=7?>=:12>7>AHAE?:819643.6C>:@	AS:i:0	XS:i:0
host|27|0|1-150=chr13:9914880-9915030	77	*	0	0	*	*	0	0	TTACAGTTGCTTTTCAGTGTCATAAGCAACTCAAAATCCTAGTCCGCTGGTGCTCATCTATACAATGACCTGATACTCTCCTTCTCCTTTTCACTCTGTCCCCTACTCACTTGGGCTGATCCTATCTGTTCTTATTCCCAACCCCCATCT	=AAACBBB@ABBGFBACBCCEFCDCFEBDDEBCABB@CDDDDDHIEGEBCCDCEGFDAECGAGFDBBB=BC?A:98<<978:=@:@@:>C@8<>;6GC@697;<?7;84;7F;A>A7<C8592G=99;AIEABI9EB204;B?:@<A653	AS:i:0	XS:i:0
host|27|0|1-150=chr13:9914811-9914961	141	*	0	0	*	*	0	0	GACCTTGTCTTCCACAGCTTCTTCTCATTCACGGTTGCACAGACAAGCTGGAATGCTGGACAAGGACAGAGATGGGGGTTGGGAATAAGAACAGATAGGATCAGCCCAAGTGAGTAGGGGACAGAGTGAAAAGGAGAAGGAGAGTATCAG	<=?@FC@>@AD?BFDEEEEECDDDD@CEIIDBB?CDCDDBCABEDHDDDDACBDBBC@DDFDFAD>CFCC?;9;:AFICEDA@=;4:>AAAC<9?<><:@CC???A?;64856CBIB699?.16B:EE./=.6;C:6:E:BD?7>55H;:	AS:i:0	XS:i:0
host|28|0|1-150=chr15:83435388-83435538	77	*	0	0	*	*	0	0	CTTCTCCATGATTGGACAACTGCCAGAAAAGCAAAGAAGCTTATGTAAGAGATTTTACCTTTTTTTAAAAAAACTTTGCCAAAGATAGTTCAGCTTTGTGCTGGTGCATTTTCTTATAGAGGCTGTTAGAAATTTGGCATAGATGTGGGA	::<??B>@BBB?BEDCEDEFIGIDCDCDDBACGGCBBC?ACDBB@CGFDDEDDECDFEDFEGCEEGBEEB<<;BCA@CBDC>??;BF8><>5:=729=5GB@BB:81EB;9;<><C?@IC8:?7?FA78>G>;=?A04@57DBI<9??GB	AS:i:0	XS:i:0
host|28|0|1-150=chr15:83435231-83435381	141	*	0	0	*	*	0	0	GTAAATATTCAATTTCAGAAACAAACTTACTACTAAGTAAAGTCAAGTGGTGGTAGATCATTTAGAGAGAGGAGGGTAGTTGCTAATTTCAATCCTAATGTTAGTCTTGCCTATTTTTTAGGAACCAAGATGTAAATGAGTCATCTGGCT	>A>BEFD?@BD@@BCDEFDDDBCDDDGIIGGGFEGGE@EBCFCBBDEEFFHEGEEFFDFFBDIGABBB<AIF?DC:78<CC:=>@88958.,>IDF>>@EA?@D=B:7/956:61838;DD=DHDC-<FA@8:G=>@HE@87CF@HE<B<	AS:i:0	XS:i:0
host|29|0|1-150=chr19:52174602-52174752	77	*	0	0	*	*	0	0	TTTGCAAGCAATAACTTTAGCAATCTATTGAAATAGAGGCATTTGTCACCTCATTTTTGCTGCTAATAAAACCAAAGTCAAGATGTTAGGGTCCTGGAACTTGTTTTAAACACAGGGCTTGAGAAATAGAGGCAGAGAGAGAGGGATGAA	6??;=?@@BBA?BA@ACADCEGFACDEEDBEDFCEEHEACEFDABEHFCGIGECDEBDGGDA<BCD?>IIE<9BIED=?@FD==G;;DGEAEDDA:>>97=>3A;0.24<D@6;<9;<?:;::9469@;GI=669:;;98@E@:::6=IA	AS:i:0	XS:i:0
host|29|0|1-150=chr19:52174743-52174893	141	*	0	0	*	*	0	0	AGGTGTTCTATCTATATATTGATCATACACATGATCACAATAATCCTCTAATCTGGGTCCTTGTTCAGGTGTCCTCACTTATTATAGATTCTTTATTATATAATTGCTTTCATGTAATTCAGATGGTCTGACGTTGAAACCTTCATCCCT	??BDED=?AABCCE@CDDEFECCCCCFDBGFEB?>AACFEHECFEBEFCCDBCDDCHHIID?CCEDACEBCBA>@@CBE9:?;BAHDHBD:BFC>73;=H?2;>>=7?7C?==<A<B?I?38,/;8FADCC9.+::8(1=<0>7/27640	AS:i:0	XS:i:0
host|30|0|1-150=chr4:103984825-103984975	77	*	0	0	*	*	0	0	TCAGAGAAACAGACAGAGATGCTGGTTGCTGTCATGGGAGAACTGCATACAGACCATGCCTGAAGCCCTCACACCCCCCAGCAATGAGGATACATTGAAAGTCAGAGAATGCAATTGGACAGACTTTCAGAAAGACCAGTCCATTCAATG	?::9;@>CBGFCHDDFHEFCDDDDCDDGEFCECDCDFEGEBACHEDDDBCEFBABC@EBCFFHF@@<=DIEGDCBADGBGFD>>AIAA=C=??<HIG=A8H;C744:AC8@FIB@515;=IE;:B95378:G<806<::4".4>ACB3'(	AS:i:0	XS:i:0
host|30|0|1-150=chr4:103985335-103985485	141	*	0	0	*	*	0	0	AAAAAAAAAAAAAAAGGACACAGCCTGTAATGATGGCCCCAAGCATACTTGAAGTATGCTAGCTATTGGGAGGTGACTCCAATGACTCCAATGACTTGGTTTAGGGACAAAGAATAACAGCTGCAAGGCTGGCTGGTCTTACACTGCATC	?><=?@EIDHIFFFFGFBCEEEDCFDCEEIGFGFBBDFCGFDDDFFEHDDDCDCFEDFCEFE@C@DA>=AB@CHFCD@@?IIC;<:HAAE>8>?8IE<>@F??EGA?9;9:7;B6<<<:9@=80897@<<IBC?BI9>DGG=<;268A=A	AS:i:0	XS:i:0
host|31|0|1-150=chr10:78421251-78421401	77	*	0	0	*	*	0	0	GTGAATCTTGTGTGTCTGACTGACTAACAACCAGGATGTTTCATTATTTTACACATTTCACAAAAAAAGGGCAACCTTAAGATTTCACAAGACATATAGATCATATCGTTTTTGTTCCCGGACAAATGTTACAAATAGAGAACGGTCATT	?@>C@BAA?CAAAAHGCBEDCDGFEFHFEFCCCEEDCDDABEIGGGFEEEEDDDDA@C?BCDEHIFCC<?EIFBCDI@<9>DB@>8?B>=?A=@?=BIB@?:F>.9AD@@BC>9;GIIDIC5348/0;2/.54893<34:8=C>7=85;C	AS:i:0	XS:i:0
host|31|0|1-150=chr10:78421469-78421619	141	*	0	0	*	*	0	0	AATAGAACAGGCTAGCCTGCAGCCTGGGAGAGCTTTCTAACTTTTAAACCAGGCTAACTCACAGGTGTACCTCAATCACTTTGCAGTGTTCAAGGCTTGGGAGCTCGCTTAGACTGACTCAGATGTTTTTCTCTCTGATTAATGGTTCAC	EBDCB?A=ABADCDBFCBFFDCFDEGFEEFDAACDDDD@CEDDDDEGEDEEFFFD?CADBCCA?EDCBBFIFCIGDCBBCIDBE?FD>?D;32=>>><FIFEDFCI99:8AA2/;H?5@7;:>18>=9?514=799CA<B?649@:60B@	AS:i:0	XS:i:0
host|32|0|1-150=chr7:21795364-21795514	77	*	0	0	*	*	0	0	GTTAAATGGTCCTGATCAAACTGATACTCTCGCTCCGTCTTAGTTGATACTTCAAACTTCGGACAAGAACAGAATCCCTCTCTATCTTTCTCTACCTGAACAAATGAGGATGCATTGTTTTGATTAAGTATTTTCAAGGATGAAATTTAT	?9>@@D<;B@>CCHGIEDBCFBDDDDCCFEGFEHHFEEIGGFHEEEDDCDFEFEEDD>DBDDE@AA>>E@DHEFGDGDC<8CBECBAH?C=6=D?>:H<FDA8;II;?B?>ADC:<@9GIIA:?A?397>727(127=ACD5AF7)1,12	AS:i:0	XS:i:0
host|32|0|1-150=chr7:21795091-21795241	141	*	0	0	*	*	0	0	CATAGCCCAGGGTGAAGTCTTTACCAAGGTCTTTCAGGAGAAGATGTCCAGTGTGGATCCTGGATTCCTCTCTAGTCGTCTTAAGGCTTCTGAGCATGACCTGTTTCTGCCTCTTTTCCTATTTATTCTCGATATGCTCAGTAGATTGTA	?<==9=BBC@B@B@=BDDDDFFFGHDD?AACDGFEDDAFCFEFFEEFBCGFDGGFEEHDDADD?DEGFDGEDADA<A>BB?IFE@HFC@?HCCID5F<GCDE>@4E??>>?A88?=7>:9;ICA><@62D@=1+<68025=5/.14;C::	AS:i:0	XS:i:0
host|33|0|1-150=chr7:18657610-18657760	77	*	0	0	*	*	0	0	TCTGTCTCCAGGCACGTATACAGTGTTCCAACCCTGGAACCTTCCCAGGTGTCTGTGAATCAGGGAGAAGCTGATGTTAATCTTGGTCATCAGCTGCTTAAGGGGACATCTTGTCCAAGAATTGAAAACAAGTGGACAGTTAGGTCTAAC	A;@B><B?ABABBCDDEEGDBDEFDDDFEFBCFEB@BCDDBADBCDCCDCEGFEGEIFEDCAAC=@ABAABCE?ECDBB==:;A=B>GIIG>@?<7/7;@A=BHF<<<>7@A;;:<::9<6.6<>><;<38D:>?H9=A>4,4-"-17=D	AS:i:0	XS:i:0
host|33|0|1-150=chr7:18657377-18657527	141	*	0	0	*	*	0	0	AGAGGGGTGTCCATAAACATAGGAAACAGATCAGGGTCTAGGGAGAGAGATCCAGGTTGGTCGGGATAGAAATGGAAAACAGTCTCCTAGGCAAGGTTATAGCTCAGAGAAACAATTGTCTGAGTGTTGAGTGACAACCCCACACAAGAA	:>=CD;>?<:>CDEBCDFEEEFHDDFFCBCEGGFFGFEECGHFHGECDCFEEEEEFB>>A=@?A=><?:@A;;7DI?D=>=CGDCIFIFIIDIHIB5//2<;??AF=>EE=>?@IE:<<=A:A919=A/5537,=-/BA37@DEHI6777	AS:i:0	XS:i:0
host|34|0|1-150=chr8:103224897-103225047	77	*	0	0	*	*	0	0	TCCAACTTTCACTTATGGAATACACAATTGTCATTATACAAATATTATTTAATTACATAATGGTTCCACTATTAAGAAATCTGTATTTAACTGATATTCACACCATATTCATAAAACACTTTATCCTAGGGACTATTTGAGAATTCTGTA	;@=A?<C<>BCCED=?ABCDECFEEEDBDFFGFEFEEFDCCCDDDHFACACDBBCBDBCDAFFGE@@>:<;;>@GFEGAA=;BAAECEBGC?=<B@C=<>>9DHCI?HIEA7<E>=>69:;3/>?1+:@05D98?:6:<;<8<92752AI	AS:i:0	XS:i:0
host|34|0|1-150=chr8:103225061-103225211	141	*	0	0	*	*	0	0	ACGATATATAATTCATAGTACGGAGATGAAAGGTAGGTGAATATGAAGCAGCTCTTCCCTGGAAATGCTTAATCATTTTTTAATATCAACACACCTTATTATAAAGACAACATGGCTTATCCAAATACAATCAGTAAAAGTACTCCTGTC	><=>BAAFCAAEDCACFDCDDB?@BCDFEIGFBCDBCD?BFHDDDDDBDBADEEEEDE@CHIFEF?ABFHD?A=G<?=AAABIAGBAA?;=DBD?>>IHD?CF;9?DIECD6@?>6CIIEFIDAC<8*2CD76<B><?41<1886=C?@E	AS:i:0	XS:i:0
host|35|0|1-150=chr3:117117199-117117349	77	*	0	0	*	*	0	0	GGCTTTTGCTTCAGTTCTTCTCATTAAAGTGAGTGGGGCAATGACATGACAGAATTTTAGACAAAATTTCCATGCTACGTAGTAATTTTGTGGCAGTAGTAGATCACTGTGCTTAGAGATGTTGGAACCATCACCAGGAGTGACCAAATC	@:@AABB?@D?GFEEGFEBBFHIFFEFECHGEDCDFEDECFGFEDGFGFDFGGGCBGGAEFFBDFDCCGIDA??BBCD?:@DBA@AB=C@G>CA3687@?G>5:<:C<<EA;>8;7@BA<;B@::7=?IIBDCCEIBAG:;08/604733	AS:i:0	XS:i:0
host|35|0|1-150=chr3:117117392-117117542	141	*	0	0	*	*	0	0	TTCATTTGGAATATTCTTAGGAGTAGAAAACAGTTCATCAAAAAGTAGATTTTTACCCTAAGAACTCAAACAAAGTAACAGAAATAGTCTTTACTCTAGGGTTGATGATGGACCTCGCTGTCCATTCTGGAATTTGTTTTGTTCCTTTCT	=;;==>?@?B=<?C??@BCFCDDDEDDDEEHFEABCGGEGFFEEDB@BDDEEDCACBB@HHIIF>=?D?GCBD;>CBFBEFB?=B<:9>?AB<<8F?A>>FBDFAED<?>IID9@IF41:7<A;48:9>EEC1.1<?=B6C<;AHI=F<:	AS:i:0	XS:i:0
host|36|0|1-150=chr7:104248694-104248844	77	*	0	0	*	*	0	0	ATGAGGATCAAAGAAGGTCATCCTAAAATAGGTCTCAGCATCACTCAGGAAGCCATTCTTGCCTTCCAGGAAAGTCTGTCTTCTTTGATGTCAGCTTCCAATGTCTCCACCTTCTCCTGCTCCTTCCTCAGCCTCGTGAGAGCTTTCTGG	<;;?@@@@?B@DGA=@BDACBBBC@BBEEEHFDC@BAGCFDBACADBCDDDBDGFEDDAEDABDEEA@9=@IHBBBEHAGG?58:BEFH?HF8>?B>HB@9:9DIB5=4436@AH:?@9;69>:F64?FIEDI995.F6:;6>;703''4	AS:i:0	XS:i:0
host|36|0|1-150=chr7:104248536-104248686	141	*	0	0	*	*	0	0	TCCACATCCTTAGACTTTGTTCTATGTTGTAAACACTACGCATCAACCTCACAGCTTTTATGAACTGGTCTCTTTCTGGGCACAAAATGTTTTCTTTGTTAGTTCACTGTCTAGGTGGAATGCATGACCGGTATGATTCCTTTCACACAG	C><=<B>C@@HE=ABEFGIGHFCFFGFCDDDCDBCDDFHGGEEBCD@CDEIHGBCBEEIGHGCIEDCCBGBABAAAAAGDD>AEI>CCDB>AB;9?BIAIE;>@=C>D>>?>=AB@;C@CIGB7:CC8?96:,353/$/98CBD?@G=>8	AS:i:0	XS:i:0
host|37|0|1-150=chr14:83575308-83575458	77	*	0	0	*	*	0	0	TATCTTAGTAAACATGTTTCAAGGTATTTTCTTTTGATGTTGACTATAAATATACACTCATTTGATTTATAGGAAAATTTTCCTTGAACCCGAGGAGGCATAGTCTGGGAATGGACTGATTGCTAACCATAAGCAAAGCTTTGGGTTCAA	:<=>?FDHIEFBDDIIIGDDCDGFFEGDB?ACDAACEEFFFCDCDEEDDDDDCDEDHHCCFED@B?A?;=@>ABB?:?@IGDEC:?I<@FC<?D>:=E=.3:A>:7?:;BI;54>=C=599C7,3AC94888,3BCIB?5:BI=9;BIAB	AS:i:0	XS:i:0
host|37|0|1-150=chr14:83575075-83575225	141	*	0	0	*	*	0	0	GTTTATCAACAATGGTATTGCTACGTACATTATTTATATCCACCCGTATCTTTGAAAGAAGTGAAGCAGCCTTAACCTTTTTAGGTCTCCCTCTTTTTTGGCTGTACAAGGCCATCATTAACTTGCTTTGTGTCTAGTGGATGATGACTG	EBA<;<>>B>?>DFHEEEEBDFGDCCDEHEEEFHEDCDDDDBCBDEEBCCDDDBDBFDHIHD>?FIIDCC?B@HEHC=>A<?:>GEECD@>CA83GDAIICI??CAH===EBD;GDDGI@.;:9;?=>@<?=?<BGB>:=99BED?<50.	AS:i:0	XS:i:0
host|38|0|1-150=chr4:35062389-35062539	77	*	0	0	*	*	0	0	CAACAACAACAACAAAACAACAGCGGCTAAAACAAATGGGATCTAAGGAGTATTTGTCTCCTCCTACCTTCTGAGACTGATAAGGATTGAGTAAGGGGAAGCTCAGATCCACTTTTCCACATATCTCTCTAAAGTATGAATGCACTCATG	8>;<<>??>A<?A@BDDGFDACIFDFEEEHFDEFDBCGFEIGEIHGFDEEEFEDDAADHGEA?AB=?BBAEFD@A>=?6GDC=A;>;?@:<>=7?GIH@:@>CHCB;CIIF>C<:5<937;E?AA>761<<<AE>;@>9?ADIA?,:97'	AS:i:0	XS:i:0
host|38|0|1-150=chr4:35062155-35062305	141	*	0	0	*	*	0	0	GAACAAAGCCTCTGCAAGATGCCCTACACACCTTGTATCCCTGGATCTAATTTCTTGGGAATGGAAATGCTTACGTTTTCCTGTCCTCAGCTTTGGAGGTCTGAGCAGAGAAAGCAGAAGCTACTCCACAGTCACATGACTCCCTCCGCC	>?>??BA==>?@=@GFDEDCEEEIHFGHECFFEHDDDCEEFBDBDFEEDCBCDDECD@C?A@BAECFH@E@AGDB<>>BHHICAC<8@=>G;=9<7FGIFIH<2@=8>FDHC=IEFA1A<<<=A=638857246:?A5?A@B@4=BCB<A	AS:i:0	XS:i:0
host|39|0|1-150=chr4:95861168-95861318	77	*	0	0	*	*	0	0	CAGTTTTCTAGCACAGTGACATTACTGGCTGGGGAGTGTAGGGCCTAAGAACTTAATCTCTTGACCAGTCTGGCACGAGAGGAGTGCTAACGTGAAGCTCCGCATCAATCAAGAAGGGGAGCAGATGCTGAAGTGTATCAGATGTCAGTA	B@>@;@AEGA@>@BDAAEFFCGFC@BBCDDDFIGFEDDEFEFEEEADAACCEEFEFFACCHEIGGGHFFCE@@A8<99;>>=IGFADDBB;ACE=HIIC?GACG>883;0>CE?ABD<@ID@:6:C63=;;>IGD69BGIF===:<0?6<	AS:i:0	XS:i:0
host|39|0|1-150=chr4:95861517-95861667	141	*	0	0	*	*	0	0	ATTGGGTGAATTATACTGTATAAAGTAAAAACAAAGAGGGGTTACTAGATGAGAAAAATAAAAAATTCGTCACTGGAAAGACACTTGATAAACTGTAATTCCAGGCTGGCAACCACTAATACTCTGAGCACTAAATGTATTCTTTAAAAT	@??<AAIH@>@=@<@?BCDDGFFGEFEEDEEEECECCB@A@DACEEEFCFGFDFEBDGFGEFEDCDIIIFD>?C<?CBEDB>ECIC>CC@94::=BA@B@>;I;EF@97?>>DHI9;<6:6355)2;>:89693?AE8;B9>HA==6@./	AS:i:0	XS:i:0
True_Junction|00|1|1-150=chr18:39199613-39199764	117	rAAV_RSV_GFP	511	0	*	=	511	0	TACTTTGCAAGAATCTGACCCTGAACAAACATCAAATCCCTGCTCTTGTCCAGCAGAAGTACACATAAATAGGGTTCAGTAGCTAGCATGTAGCAGGCTGCTTAAAGAGCACTGATTAGGAAGGTAGGTTTTAAGTGCAAGAAGTCAAAA	0&5+/;;47=@AIBIF=;:6>?AAB@IIIIF??8:IE@CCD>@AD@<@>=I>BD9;669GDEH=A;ICI?=B=A=9=<D<=@BACCEEGCDGDBECEEDEEEDDHFHEFGDDDCBFIFFDC@DEEFCCCBEEEEIHHA?BABC@?<@A?A	AS:i:0	XS:i:0
True_Junction|00|1|1-150=AAV_RSV_GFP:509-660	185	rAAV_RSV_GFP	511	60	150M	=	511	0	GTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATTCTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGAT	+51?HI>>DIBFE<34759697:5BF3)9>7;;:D>CIE@A?A>??CB=<>IGA<9FDIAG9@;B<98<@>BB?ABIIIA?I@CCCC?>CB@<ABCDDBA@CEFHDFEEDCBEHEFFDCBEGIGECDCBDFEEDDFHF?@>?<@@@A>EE	NM:i:1	AS:i:145	XS:i:0
True_Junction|01|1|1-150=chr18:39199566-39199717	117	rAAV_RSV_GFP	523	0	*	=	523	0	GTCCAGCAGAAGTACACATAAATAGGGTTCAGTAGCTAGCATGTAGCAGGCTGCTTAAAGAGCACTGATTAGGAAGGTAGGTTTTAAGTGCAAGAAGTCAAAAAAAAAAAATGATGTGTCCCCTAATCCCTGTGTTAGAGTTGATAAGGA	9;B8+:9)-.*@ED?E<B?>11@=<;FB5:;9<8;B@;=G@B?937><BICEB<@CB><HD7<DEDD<FIDI@A?DEIGCHHHCGDEGIEEFFIFHFEECDFEEEEEFFFEEFHIEEFGGGIEDCBEF@BCDECBBBA@=@>?CHG@?@=	AS:i:0	XS:i:0
True_Junction|01|1|1-150=AAV_RSV_GFP:521-672	185	rAAV_RSV_GFP	523	60	150M	=	523	0	TTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTA	08865;98AEB>D?B<:30788,6959;)1:;)3>95HI@=CHC=>>I?@>AEAB=?<B@FCD<?<8=ABBDBA?<9AB>?GFEBCBABFFBEEDEDDB@DDDDDECHEEFHGFGFCBDCCDEDDDDBB?BB?@BBAB?@ABD<@?@>?<	NM:i:0	AS:i:150	XS:i:0
True_Junction|02|1|1-112=AAV_RSV_GFP:473-585|113-150=chr18:39199727-39199766	121	rAAV_RSV_GFP	474	60	38S112M	=	474	0	TTTGATGTTTGTTCAGGGTCAGATTCTTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATGGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACG	D89GID?9>7;4:2:62(=?62A:;>8FGB;4:B>:6?9=H?>>=9<<DI9B@C7A>??A<EFH@E>8FD9;==FDFH;BDBCDFHFHIGIFGCDEEDCEDEFGCDCDCEFGEBBBDCEEEBDEEEHDDCEEFFCAFAA?>>?>>>>?86	NM:i:1	AS:i:107	XS:i:0
True_Junction|02|1|1-150=chr18:39199516-39199667	181	rAAV_RSV_GFP	474	0	*	=	474	0	CTGCTTAAAGAGCACTGATTAGGAAGGTAGGTTTTAAGTGCAAGAAGTCAAAAAAAAAAAATGATGTGTCCCCTAATCCCTGTGTTAGAGTTGATAAGGACAGAGTTTTATCTAGACCTAAAAAGTAAGCCAGGTCTACTATTTCCTGGG	CHD7:-8;379C:;;;7=?:9;442:<9<=>?GA>?B59473<4,2A?>?@G>C;DEG@<DCFEIIECEHDA?CD@:EEHIEEHH@CBA>CFHEEGGEEBDCBCDEDHEEEEFGGCBEEECAAFHFDBEEFFEC>EABCD@?AAA@?>>>	AS:i:0	XS:i:0
True_Junction|03|1|1-138=AAV_RSV_GFP:473-611|139-150=chr18:39199753-39199766	83	rAAV_RSV_GFP	474	60	12S138M	=	474	-138	TTGCAAAGTACTTTCGGATGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGG	5><BCG7:47>7A@B:?./*.45;F@?@?98@=84A?@C8>6@<;1254;;49C=GDIII@IIHHBABADB;;@?A?ABFIDFGC@AGBAA>CDDBBCEEEFHEDDICDDBED@ACHEEEECBADCDDDEFDEEB@CBAC=B?>;=;>A@	NM:i:1	AS:i:133	XS:i:0
True_Junction|03|1|1-26=AAV_RSV_GFP:473-499|27-150=chr18:39199641-39199766	163	rAAV_RSV_GFP	474	60	124S26M	=	474	138	TTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCTGGGATTTGATGTTTGTTCAGGGTCAGATGCTTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCC	>B@D@AA?>AA@CGED@CEEDIGIFFEEDECDDDBFEEFEEEEFEGCCDECGACEDDDB?A?BCFIFC>BIFE@BGDCC;@DCD?CBADEHA?:4=@?I>DI???D@=IGFI8@<B?559F>;;5>=E:5@C:><>:>A=?IA<A==3.8	NM:i:0	AS:i:26	XS:i:0
True_Junction|04|1|1-150=AAV_RSV_GFP:513-664	83	rAAV_RSV_GFP	515	60	150M	=	474	-191	TTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTG	357,8B8?GIDC><@<9:87E@37@7=<I?<;9=<:ADIHD;F?FF:@ACH=9:8DC;@F?=;B>>;:>I?<>9<>:EGD@IDECCB@B@BA@BFEEDCEEFEFHECFGFCBA@?@ABDDDCBBCGHACEEDEEFAC?<=>A@?@AAB@=	NM:i:0	AS:i:150	XS:i:0
True_Junction|04|1|1-19=AAV_RSV_GFP:473-492|20-150=chr18:39199634-39199766	163	rAAV_RSV_GFP	474	40	131S19M	=	515	191	ACCTACCTTCCTAATCAGTGCTCTTTAAGCAGCCTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCTGGGATTTGATGTTTGTTCAGGGTCAGATTCTTGCAAAGTACTTTCGGTTGTACGCGGTTAG	?CBB?>?A?AD>B=AFIGFDDDDEEBCDBEDBCDBCDCDCBBCBCDFECDCCDEDECCDFED?BA=ABBCC:DDC?8@A>EEGIDEDDI=C=E>18IDEHGA53><7C@E>978AIDE@EGI@?<40B<@A;A>F9I?@A;?7797:':?	NM:i:0	AS:i:19	XS:i:0
True_Junction|05|1|1-128=AAV_RSV_GFP:473-601|129-150=chr18:39199743-39199766	121	rAAV_RSV_GFP	474	60	22S128M	=	474	0	GGTCAGATTCTTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATG	@=3<>6C356@:>E:97?.>?GGC=;CIAFF=?;>95<=9DI?A<940146>A>?;7607;;:AC?>8:?<==?=8?@@=>9@@@DEDDFA?CDFHCCDFDEFGC@EEEEFFGFC>?@DACACDEEFEEEBDDGGDDDFBBDBC?BA>>@	NM:i:0	AS:i:128	XS:i:0
True_Junction|05|1|1-150=chr18:39199589-39199740	181	rAAV_RSV_GFP	474	0	*	=	474	0	ACAAACATCAAATCCCAGCTCTTGTCCAGCAGAAGTACACATAAATAGGGTTCAGTAGCTAGCATGTAGCAGGCTGCTTAAAGAGCACTGATTAGGAAGGTAGGTTTTAAGTGCAAGAAGTCAAAAAAAAAAAATGATGTGTCCCCTAAT	@<369AI??716>G=7<DA?@I@E03-08>724BBHB>@8;E><629?4;4<??AI83?6CDFE>?;AIIHIB==:=<>E>CEGCECBCA?CCDGHHFGFEIFGECBADCCBCEFCFEDBB@CGHEEDFHCB@AB9>BAACCAAABDB=@	AS:i:0	XS:i:0
True_Junction|06|1|1-3=AAV_RSV_GFP:473-476|4-150=chr18:39199618-39199766	117	rAAV_RSV_GFP	500	0	*	=	500	0	GAAAGTACTTTGCAAGAATCTGACCCTGAACAAACATCAAATCCCAGCTCTTGTCCAGCAGAAGTACACATAAATAGGGTTCAGTAGCTAGCATGTAGCAGGCTGCTTAAAGAGCACTGATTAGGAAGGTAGGTTTTAAGTGCAAGAAGT	4C>?EC;7<9?<;I:=9@2?IC?=8HICD>FB85==<=ID<6/;??>7(BIB<G?@<DE??>:@A@?8=9==><BB=ABBA@=BA<D>A>@IDC@BFIDEFCACDDDDDDCFDDDFEEEEGEECCBDDDCCDDCAA?DDAAAC@<9@=?<	AS:i:0	XS:i:0
True_Junction|06|1|1-150=AAV_RSV_GFP:498-649	185	rAAV_RSV_GFP	500	60	150M	=	500	0	CTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGTGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTTCATGCCGATTGGTGGAAGTAA	7GC<;97;:;AIC:3>?;??.96:E;;5BF>@<9I=<;=G6B@ACI??;=;B9=?G@DIEABF?B@A=>B=7>:A@?@=GFADF@@@DDEIDDEGDFBDCCEFDDCACDCDDDDFHCBDEEEFEEBFEFHDCBDADFCDCC=:@@?@>B>	NM:i:2	AS:i:140	XS:i:0
True_Junction|07|1|1-150=chr18:39199556-39199707	117	rAAV_RSV_GFP	474	0	*	=	474	0	AGTACACATAAATAGGGTTCAGTAGCTAGCATGTAGCAGGCTGCTTAAAGAGCACTGATTAGGAAGGTAGGTTTTAAGTGCAAGAAGTCAAAAAAAAAAAATGATGTGTCCCCTAATCCCTGTGTTAGAGTTGATAAGGACAGAGTTTTA	60?<:;:43>B32*-4E<?>ICH:5:6;07:8>A;87I@=;8GB39A>D<CIIGID?BDDGDB:7<BC=@>E>@?BHGA;>CGEC=>=?BCCACDDDDFFEEEFIGEDDDGHIGFGHFECCCBCCDEGFGFGEECGDHEDA><<;:BEEC	AS:i:0	XS:i:0
True_Junction|07|1|1-52=AAV_RSV_GFP:473-525|53-150=chr18:39199667-39199766	185	rAAV_RSV_GFP	474	60	98S52M	=	474	0	CTGCTACATGCTAGCTACTGAACCCTATTTATGTGTACTTCTGCTGGACAAGAGCTGGGATTTGATGTTTGTTCAGGGTCAGATTCTTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTG	&"01423459G>;88@2-73;<?<AF;99:AGII>75.?<7;9481>A85<:?B:>?F<8ABCEIIHEAA?=:<DIEBA?A?D@D?B@A@CCCEGEEEFDEEECEEFGDCACFFCACBDHGABDDDCBCDDCFEFADDBA@CE==?:<<<	NM:i:0	AS:i:52	XS:i:0
True_Junction|08|1|1-97=AAV_RSV_GFP:473-570|98-150=chr18:39199712-39199766	121	rAAV_RSV_GFP	474	60	53S97M	=	474	0	GGACAAGAGCTGGGATTTGATGTTTGTTCGGGGTCAGATTCGTGCAAAGTACTTTCGGTTGTACGCGGTTAGGAGTCCCCTCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACTCTTGTAGTCT	261)8>6?49DEH4::>9:745)&51!7:;<AI?:9B=?B;<>4@DGIF@>=8A;;:<8?8>>;?B>::<GDFC?CDFDAD@DD?AGFHEC?CHHHFABDFDCFGBBGGGDCEEHGE@DDC?@B@FGDCDEFFCFEDBB??<ABAD@ACB	NM:i:0	AS:i:97	XS:i:0
True_Junction|08|1|1-5=AAV_RSV_GFP:473-478|6-150=chr18:39199620-39199766	181	rAAV_RSV_GFP	474	0	*	=	474	0	CCGAAAGTACTTTGCAAGAATCTGACCCTGAACAAACCTCAAATCCCAGCTCTTGTCCAGCAGAAGTACACATAAATAGGGTTCAGTAGCTAGCATGTAGCAGGTTGCTTAAAGAGCACTGATTAGGAAGGTAGGTTTTAAGTGCAAGAA	98ICGIEIBIIBD:=IGB:96H;B?;EBHA4@4;><<:;9;D59EFE@>I9@@@@A;<9C@@I?ACGEDCGHI@@@DFFIFAAABBFDDEGC@DDEFFFFDCBCBEDCBDCCCEBDEEFDDEFEEGFEFFDDEEED@BBADD@@@@C?@F	AS:i:0	XS:i:0
True_Junction|09|1|1-150=AAV_RSV_GFP:540-691	121	rAAV_RSV_GFP	542	60	150M	=	542	0	TGTAGTCTTATGCAACACTCTTGTAGTCTTGCAACATTGTAACGATGAGTTAGCAACATGCCTTACCAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTC	@<6989?=<5)627>>04.:=DC70/0.97@GDAD;AA><<>AHI3==BF@E?3;9EB9?GBGCIFGEG?9A@?EEHBIFD<?DBA?<@EECBDDEEFFEDEEEDCGHAAAFFFHGEEDFDHF@BBDCDEEDDDDCEGGFCE@?AABDBF	NM:i:3	AS:i:135	XS:i:0
True_Junction|09|1|1-150=chr18:39199573-39199724	181	rAAV_RSV_GFP	542	0	*	=	542	0	AGCTCTTGTCCAGCAGAAGTACACATAAATAGGGTTCAGTAGCTAGCATGTAGCAGGCTGCTTAAAGAGCACTGATTAGGAAGGTAGGTTTTAAGTGCAAGAAGTCAAAAAAAAAAAATGATGTGTCCCCTAATCCCTGTGTTAGAGTTG	783,2868468506417981A87@?@D:IFGB82ED?;/<F@<>>???59A8;C9>;9>;;CHI?A85>ABIHFDBBBBEIBDFEGE@E==CD@CACEDFGFGFHHGDCACCDBGEEBCCCFFCDDCEECCDCB?CBA@@@>:=@@AABA	AS:i:0	XS:i:0
False_Junction|00|1|1-150=chr6:55819046-55819197	117	rAAV_RSV_GFP	1188	0	*	=	1188	0	ACTGCAGGGTCCACTTGCCCTCCTGAGTAGCAAGGACTTCCTGATAGCTACAGGATGCAGCTAGACTGTTTATGCTCACATCAGCGGTGAGTCCTGCTTCCCTTGGGGATGAGCGAGCTGTTATTGCAGCAGAGCAGGAACAGAAAAAGG	76949@@>@@95A=?:<E<=A?3<871G;=96AFHIE:B@:A94IIDHGDC?<FIAHIB=GDGBDB@DIEFE<A@>AIAGEA><<ABAAD=@@CDGHEDDDCDDDGEECADIEHCCABHFEFDFFFFFGIIIIHHGCECBA>AABBB>>9	AS:i:0	XS:i:0
False_Junction|00|1|1-125=AAV_RSV_GFP:1188-1313|126-150=chr6:55819199-55819225	185	rAAV_RSV_GFP	1188	60	24S126M	=	1188	0	TCAAGCAGCTGAGTGGACATTGTAGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCAC	83/-.1329;;9?12:7:73;91G:D<5.><F;69=<@>=ED6126@?<9D@<@A88;?9:<AD>F??=A@A@;DFE??<?==?@AFBABA?>ADFDEECEDEEFEFEEDFDGFEFGEEEDDFEFEFDCDDDCDFEF@A@AA@B=;>?<:	NM:i:0	AS:i:126	XS:i:0
False_Junction|01|1|1-150=chr14:17049267-17049418	117	rAAV_RSV_GFP	170	0	*	=	170	0	TAAAGCCAAATTGTATCTTGTTCCATAGAGTAGTGTGGTGTAGAATGGGTCCCTTGGCCCTATGACTGTTTTGTGTCAACTGAAATCTGGGTCTCTCCTTGGATCAATGGCAGTTCATATTGGTTAAGCTTTAGGGAAGTTCTTCTGCAT	EBICBA?=955=I=<9A3>A5;;><C=E2<;=7;93AA=?C@:>=82@>D>B@@7>D96;=@?F??HICE?@?A?AAECDCC@?A?EAB??DAFGDABEECCECGDCEDGDDDDEEEEGCDEEEGCBDFECEEFFGGDFDAB@EG=@9>@	AS:i:0	XS:i:0
False_Junction|01|1|1-90=AAV_RSV_GFP:169-259|91-150=chr14:17049435-17049496	185	rAAV_RSV_GFP	170	60	60S90M	=	170	0	GCAAATCTTTTTTTTTAAGTAGGAACAGCATCAAAAGCAAATTTTAAGAAAAGAAGCCTGGCCATGCTCTAGGAAGATCTCGACGCGTCATGTTTGACAGCTTATCATCGCAGATCCGTATGGTGCACTCTCAGTACAATCTGCTCTGAT	.;9F7475B>I80;>27EEHE?784@?A?B@659IFI?505;5=7959>B;C39IDF>AH;BAAE@@ABCBCBC>;@?@CB@>:;:=DEFEDBFDFHEFIFEDDGEDC@>@@EEEECCEEDDDDDDDFBEDFDADEGGDE>DEDH@>?=A	NM:i:0	AS:i:90	XS:i:0
False_Junction|02|1|1-150=chr19:28045506-28045657	69	rAAV_RSV_GFP	1225	0	*	=	1225	0	TCAGCATCGATGTCTTCATCAGTCACGCCCCAGGAAACCTCAAAGTGTTAATGTGTTATTGGTAGAGGTGTTAACAAATGCTATCCCCACCAATAATGGGCAGTCGAAAGCACAGCCCCTCTACTGTCTGCCCTGTCACTTACTTTTTGG	@@@??AAA@ACCCDAACFFFEGFFEEEDGGFDDBCBACEEEDBCDEEBAEEEEDDDFCCCDDCACCDDA?BAD@AAACBDBBD@9F<:=7???6A>CECIID>@B@C?DGB9>CACA<:;928IHIE9=@-1:5::9B@<6;B5@E?<:C	AS:i:0	XS:i:0
False_Junction|02|1|1-107=AAV_RSV_GFP:1224-1331|108-150=chr19:28045988-28046032	137	rAAV_RSV_GFP	1225	60	107M43S	=	1225	0	GTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGATGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGATGGCTTATGTTGCCTGTCATAATTTTTTGAAGCCCAGCATTTT	?<AAABF@CB>>A@@@AACBCDCEFGFEDEDDDAFAEECFAEDDCDDDD>?ACEFFGEBB99>A@BBCBFGFF?BB=C>GA99<ABA>GAA=><B;B<>?559:<=AIA;4;;47=A<<7B45<<A/293<@II@:>@G79C:D=B;?FG	NM:i:1	AS:i:102	XS:i:0
False_Junction|03|1|1-150=AAV_RSV_GFP:1260-1411	83	rAAV_RSV_GFP	1262	60	150M	=	1161	-251	AGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACT	74/+/>9;9<18<<@6=7>@:.:=DE@CC:D32<::9?C:CIDC0@CHECG?76:5<7;=@=4@FDE;:59=A9?BFBAEA@CEA?BCAEIDDEFFGECCAFFCFFFEFFEFHFGGHFFCFEFEGCCEEEFEFDBGEHDA@CAB?=@@=A	NM:i:0	AS:i:150	XS:i:0
False_Junction|03|1|1-34=AAV_RSV_GFP:1160-1194|35-150=chr1:97749461-97749578	163	rAAV_RSV_GFP	1161	60	116S34M	=	1262	251	CATGAAGTACTATGATGACTGTGCAAAAATTCTTGGGTAAGCATTTACTACTGAACCTTCCTCCTGCGTGCATTTGAGCATGTGTTAGCCATGGGAACTTTTTGATCACTTGAGGGCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGG	?A>>97<<>EHIFFEFIGICACDDCDHFECFFEEEDDBBC@@DDFAED@BDBCBCBD?BCCAEAD@CCACBA;DAAAGCH>@DCBC>>IH=B;A@=>=;7=@=IIDIIIFII;9C>=;>?=<ECIIIB,6?;A@B>;:>799145$0:D6	NM:i:0	AS:i:34	XS:i:0
False_Junction|04|1|1-150=chr13:43098212-43098363	69	rAAV_RSV_GFP	1059	0	*	=	1059	0	TCGTCAATACCAGGGTGGGCAGGACTGCTTTCTGCATCTCTCATCTCTCCCTCTCCTCACTTCTCACTTTTATTCTGTCTGTCATTTTCTACAATGTTACATTAGGCAACCAGATATGCTGGCATTTTACTCTGTCCATGGAGAAGTCTT	?;@B@FCDCB<<>BEBBCCDEBCDBCDDDEEEEFEHFCEEBCCDCDAC@@ABCEHFDEGE>>ADFBABC@B@BBG@A@?;>8=<>:CB9D@<G@A:A=>E99CA=;AB?B@9514:=8069?II8,,5%898:476<B58GAB79:D/05	AS:i:0	XS:i:0
False_Junction|04|1|1-76=AAV_RSV_GFP:1058-1134|77-150=chr13:43098149-43098224	137	rAAV_RSV_GFP	1059	60	76M74S	=	1059	0	GCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCGCCTCCCATTTCCCAGACTTTCCTATAGAACAAACAGAGCCTGTAGTATATGGAGGCCCATGCAAGACTTCTCC	HC@C@A?@@A?<BDACDDDDCDBBCDDDCCBBBCBCDCBDDADEFCIHECBBAACDCB@@C?AGHCEEDC>;BB@?@FEC;A=A<A>6AA=:=C??BC8DGBAFBH>8;8<58<8C@5<A7;>5D529=;DGIDCI<8:AFD;E;DC@42	NM:i:0	AS:i:76	XS:i:0
False_Junction|05|1|1-4=AAV_RSV_GFP:1708-1712|5-150=chr18:30329153-30329300	69	rAAV_RSV_GFP	1416	0	*	=	1416	0	TCTGCTCTTATGGTAGCTATGGTACCTAGGAGGGGGAAGAGATGGGACGCACACAGTATTAAAGACTGTCCGAGTCACGAAAAAATGGACATGTTCATGGTGTCATTTCTCCTGGGACTCACAGTGGAGCAGATGCCCAGCCCCATGTCA	?=@?@DBAAADF@BCDDFHEGIGIHGFEDEIGEDDCCHHFHHEEDFFBCDDDEBBECCBEEC@BBAD?AHCB?AHGB@CI9=8;>?<>=BAEHI<F?>4>6:>85,5BAFCCH8<:9;25:95?;9?6180988>@27?AC682<9+302	AS:i:0	XS:i:0
False_Junction|05|1|1-150=AAV_RSV_GFP:1415-1566	137	rAAV_RSV_GFP	1416	60	150M	=	1416	0	GGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGTTCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCA	FACDFIIEEFFGCDCFFFDCGFEEDHFEFFEGEEEGDEEBDDDDDEEFGHEFGFBCB?<A@@D?AABB8=>@@B??=?BA?<75C=?A38C9=99133435;<>@C>>=ID==<C@51/7=<8G9FGFI9<81//:959<=5768@<CBB	NM:i:1	AS:i:145	XS:i:0
False_Junction|06|1|1-46=AAV_RSV_GFP:730-776|47-150=chr2:59284184-59284289	99	rAAV_RSV_GFP	730	60	103S47M	=	775	195	GGGAAATTTGTAGGGCTGTTCAATTTTAAAAGTAAATTCTAAATGATTTTTCAATCATTTTTTTTTTAAACAAAAGAGGCAGATTAACCATTTTATGATGCAGGAGATATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTG	FAA>?>:DF>BIFEHGGFDDEECCCBACBEFEFEIHEFHFFFEDCDEIIGFEDEEEDB@BAA@@=CCA@IHGABGE@DGFCDH@9=@@4:6<8ABA:;<?7;:18;4C<=B49>96FBA@9<EA?;=<9:656)2.+16042<C8956'6	NM:i:0	AS:i:47	XS:i:0
False_Junction|06|1|1-150=AAV_RSV_GFP:773-924	147	rAAV_RSV_GFP	775	60	150M	=	730	-195	TGACCATTCACCACATTGGTGTACACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGCTGGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGAC	>;>99?859?=>537,!3-<=>HIIF788<?GB=?ABADG9=;8AA:=C>A?=A9;D87BDC@@=73>ADC??CA=A@AABIDE@FBA>FED@CEDBBFGFFFFEDA?>DDCECEEDECECEEDEBAAECAEEFHFA>BA>??=@<9656	NM:i:1	AS:i:145	XS:i:0
False_Junction|07|1|1-17=AAV_RSV_GFP:1233-1250|18-150=chr10:17253821-17253955	69	rAAV_RSV_GFP	1104	0	*	=	1104	0	ACTTTCTGGTGGCTTTGAACACATTGGTTATGTTCCTAAGCTAGCATTTTCTTAACTGTCCCATAGAGTGACAATGTTCTCAAATTTCTTCTGATGATATTCTGTTAAGAGTCACACCACAATAGTAAAGATTTCGGGGTAGCGGCTGAA	<@=BCD@;CCBAFAFGFCBCEHGGFEHFBCACCDCEFEEEGEFEEGIEAAEDDDEEDDGD=CBBCCFBD@7>CGD@B@BBDAIIE;E@9BHA94;5:@B=AE=C>.<=?A9?BC9@CDBI9ABA:8:=?=CEI??@<7BF?.45<6639:	AS:i:0	XS:i:0
False_Junction|07|1|1-147=AAV_RSV_GFP:1103-1250|148-150=chr10:17253951-17253955	137	rAAV_RSV_GFP	1104	60	147M3S	=	1104	0	CAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGAAAT	>=?<>B><ECCF?ABIGFGHFDDDFACDDEEEECCBCEHEEECDDHCDDAACFFEEDEFIIGDCCFECIFIDCGD?@?DIHF<=@EBBGII=>?C@@B<AA@>>C<69=IIAEGGHB=DA>I>?E>A8@5=BH80==?=1@<99474=83	NM:i:0	AS:i:147	XS:i:0
False_Junction|08|1|1-150=chr2:59284477-59284628	117	rAAV_RSV_GFP	730	0	*	=	730	0	TTTCTTGAAGGACCTTCCAGGTCATCGAGGAGTACACAGTCAGTTTAGACAGTAGGGCCCACGCTGTGTTTACAGTTTCTGGAGTAGTTTGCATAAGTGAAGCTTAATTTGTTACTGGCCATGGTTAATTGCTTCAGTCATTGAAAGTTC	*2852-0:.--4+69:/;:=46.2:616:AB;DAB?A;8D?;;<9:F=HGCAGDBE@I6?;;ADGIHAE=?=C@GFIDC@C@?<8?@ADEIGBACCDCAGEGFFGEFEBGGFFFFFICCBDDHGIEFDEGFGDCEDAACDDE@@BD?AC?	AS:i:0	XS:i:0
False_Junction|08|1|1-117=AAV_RSV_GFP:730-847|118-150=chr2:59284184-59284218	185	rAAV_RSV_GFP	730	60	32S118M	=	730	0	AAAAGAGGCAGATTAACCATTTTATGATGCAGGAGATATTGTATTTAACTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTTGAGATCTGCTTCAGC	EA@42??>3+<68CIFHG=:40:<A>B76>E<2A?=B?B8@AE=34:6A?<:@DAII>7CFIDFGACH?<CHA<=E?CCACAB>BFABDHDDCCAEEFGHIHHEFFDCFGHEECBBCDCFEEFEDECBDGDFFEEE@AACFBAC@B@@BF	NM:i:1	AS:i:113	XS:i:0
False_Junction|09|1|1-150=AAV_RSV_GFP:1744-1895	73	rAAV_RSV_GFP	1745	60	150M	=	1745	0	CGGCCGCAGTTACGCTAGGGATAACAGGGTAATATAGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTAT	D??A?B?@?@?ADG@FEFDEFHFHFEDDDDDACDACDEDDABCDFEFEECBCEFEFCDEA@A?A@>BEB?CCC?FF?BB@FCG>>:<CH>D<<>FB@67@46,5597=@>BD?>AIFIB9@CEB@79B@=4<6:>@9>=@=88842;I?3	NM:i:0	AS:i:150	XS:i:0
False_Junction|09|1|1-10=AAV_RSV_GFP:1886-1896|11-150=chr6:17936549-17936690	133	rAAV_RSV_GFP	1745	0	*	=	1745	0	CAAGGACATTCACTGTGAGCCCATGCACAGGGGTATTCACTGTGAGCCCATGCACAAGGACATTCACTGTGAGCCCATGCACAGGGGTATTCACTGTGAGCCCGTGCACAGGGATATTCACTGTGAGCCCGTGCACAGGGAAATAAAGCA	B@BFCFBAA@>D@A?ADDHH@?ACDDDDDDFGFEDGFEAFEEFDEEGGHGFFFC@BCACCCFDDF@@ABBDCC?B:B=BHHEBAIF=?@@?:;>;B;HCA@D>DFFCCGEF9<DEIGI>9=43E;=9=69472;7@=IB><059=A:?9;	AS:i:0	XS:i:0

[M::main_mem] read 200 sequences (30000 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 39, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 326, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (329.29, 87.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::worker2@0] performed mate-SW for 4 reads
[M::worker2@3] performed mate-SW for 4 reads
[M::worker2@2] performed mate-SW for 6 reads
[M::worker2@1] performed mate-SW for 6 reads
[main] Version: 0.7.5a-r405
[main] CMD: bwa mem -t 4 ./bwa_index/all.fa ../test/fastq/100seq_R1.fastq.gz ../test/fastq/100seq_R2.fastq.gz
[main] Real time: 0.009 sec; CPU: 0.016 sec

It's not. However the standard output can be used as a generator that yield the stream line by line with next() Sam format can thus be easily parsed and stored in an adequate object.



In [10]:

    
from subprocess import Popen, PIPE
cmd = "head ../test/test.sam"
proc = Popen(cmd, shell=True, stdout=PIPE, stderr=PIPE)
sam = proc.stdout

print(sam.next().split("\t"))
print(sam.next().split("\t"))
print(sam.next().split("\t"))
print(sam.next().split("\t"))









    



['@SQ', 'SN:AAV_RSV_GFP', 'LN:2216\n']
['virus|000|0|1-150=AAV_RSV_GFP:198-348', '99', 'AAV_RSV_GFP', '199', '60', '150M', '=', '321', '272', 'ATGTTTGACAGCTTATCATCGCAGATCCGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCA', '?C@?<==@BFFFFFGIGBCCCCDDCAFFFEFDCD?BACBDEIGCFFFECADBDDDECBB?@@CCCEHEAGEEFGCBBB<D;>BECCHGF@7>:FIH9B<7?D;96;B;>?7DICA?:?927165:;9593::;C7:G=;;22#*;:>5A?', 'NM:i:0', 'AS:i:150', 'XS:i:0\n']
['virus|000|0|1-150=AAV_RSV_GFP:320-470', '147', 'AAV_RSV_GFP', '321', '60', '150M', '=', '199', '-272', 'CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTATGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATTCGCGTATCTGAGGGGACTAGGGTGTGTTTAGGCGAAAAGCGG', 'C;06>?63+0(38<864<A?97@CIII@;1/*9@B@;@=I<>6:?2<D29B9<@IBF<@@IHDA89<AA<A??<@>?EG<B>?EECCB;A@>ACCD@DCFEDDDDDDDECDDDBBBAAACFDDEEEBEEEBBB?>@BFHIEE@@D?B=@B', 'NM:i:1', 'AS:i:145', 'XS:i:0\n']
['virus|001|0|1-150=AAV_RSV_GFP:1213-1363', '99', 'AAV_RSV_GFP', '1214', '60', '150M', '=', '1321', '257', 'TGCCCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGT', '?==?@??B?>=@B@BECEEEEGDDDBCDDDBCCACEEGFIFEEEEBEEFIFEFEEGCFEBCBAB>@DCAD=C@ACCEE><CG?FII@GH=??DFCIIDA=A=@D>DIB>;=>75529:;9=9<6<=+;49<=<;78A3<;;:?:2:<38>', 'NM:i:1', 'AS:i:147', 'XS:i:0\n']

TODO:

Create a parser for samfile/generator
Create a samFile class
Create a samReccord class
Create a samHeader class
Create a samFilter class

just use pysam by in this case it is necessary to write the sam file somewhere on the disk before parsing (temporary file?)

2014 06 30

I will try first the easy way: ie using pysam BWA output secondary alignement in the list which confuse pysam

Otherwise pysam looks great = parse sam and creat coupled_sam object containing all possible primary and secondary pair

2014 07 21

Ther was a bunch of changes that I did not reported in the notebook. Here is a short description of this modifications

@RefMasker

The package folder was removed
All classes related to Blast were merged in the same py module file = BlastnWrapper
The organization of BlastnWrapper changed. Now a database object needs to be created and attributed to a Blastn Object. A database object can be created from a existing reference or by indexing a reference fasta with makeblastdb. The Blastn method align can finally be used as many times as desired with different queries.
The RefMasker class was dismissed and transformed into a more simple function library to allow future implementation of multiprocessing (which is pretty complicated with classes). Mask method from refmasker is able to to all the job by itself from db creation to editing of the reference sequence; It also create local folders to store

@FastqQualityControl

The package folder was removed from now since it contains 4 classes... But it may be done in the future if all file are merged.
The original python paiwise aligner used in adapter trimmer was very slow. To sharply improve the speed of alignment my brother Clement Leger and I managed to write a pretty evolved wrapper for a rapid striped SSW C SIMD library forked from mengyao githud repo. We requested a pull back after edditing thant was accepted by the repo owner. There is still some work to do to adapt the API for a more general purpose by it is well adapted for my project for now. It takes around 30 to 45 minutes for 4 primers around 3 millions of paired end reads.
To increase further the speed of the analysis I shall transform the FastqFilter class in a parralelized function same as for RefMasker.

@ShortReadAligner

A class reading fasta reference files was implemented to generated a collection of sequences name for each source reference
FastaReader can also merge references in a single fasta file if needed to allow indexing of a common reference.
The package folder was removed
Same as for BlastnWrapper a file BWAWrapper file was created and the organization now similar way. The modified classes had not yet be tested...



In [ ]: