Example slide show

(NBIS Python course)

Sergiu Netotea

Notes:

http://www.slideviper.oquanta.info/tutorial/slideshow_tutorial_slides.html

General overview:

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CRISPRs!

• Clustered
• Regularly Interspaced Palindromic Repeats
  • separated by short (30bp) bits of non-coding DNA (spacers)
  • derives in microbes from exposures to a bacteriophage virus or plasmid
  • the process being guided by two highly conserved enzymes, Cas 1 and 2.
  • 24 to 48 bp, with dyad symetry but not truly palindromic
• are contained in 40% of the sequenced bacterial genomes and 90% of sequenced archaea
• New spacers added always at the leader sequence end (thus it provides a mechanism for evolutionary studies in prokariotes).
• Pairs with Cas9 nuclease can be RNA guided for precise genome editing. (Yay UmU!)
• The most common microbial defense system. Co-evolution bacteria-viruses.