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%matplotlib inline
This tutorial covers the basics of creating and working with :term:evoked
data. It introduces the :class:~mne.Evoked
data structure in detail,
including how to load, query, subselect, export, and plot data from an
:class:~mne.Evoked
object. For info on creating an :class:~mne.Evoked
object from (possibly simulated) data in a :class:`NumPy array
<numpy.ndarray>, see
tut_creating_data_structures`.
:depth: 2
As usual we'll start by importing the modules we need:
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import os
import mne
Evoked
objects from Epochs
:class:~mne.Evoked
objects typically store an EEG or MEG signal that has
been averaged over multiple :term:epochs
, which is a common technique for
estimating stimulus-evoked activity. The data in an :class:~mne.Evoked
object are stored in an :class:array <numpy.ndarray>
of shape
(n_channels, n_times)
(in contrast to an :class:~mne.Epochs
object,
which stores data of shape (n_epochs, n_channels, n_times)
). Thus to
create an :class:~mne.Evoked
object, we'll start by epoching some raw data,
and then averaging together all the epochs from one condition:
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sample_data_folder = mne.datasets.sample.data_path()
sample_data_raw_file = os.path.join(sample_data_folder, 'MEG', 'sample',
'sample_audvis_raw.fif')
raw = mne.io.read_raw_fif(sample_data_raw_file, verbose=False)
events = mne.find_events(raw, stim_channel='STI 014')
# we'll skip the "face" and "buttonpress" conditions, to save memory:
event_dict = {'auditory/left': 1, 'auditory/right': 2, 'visual/left': 3,
'visual/right': 4}
epochs = mne.Epochs(raw, events, tmin=-0.3, tmax=0.7, event_id=event_dict,
preload=True)
evoked = epochs['auditory/left'].average()
del raw # reduce memory usage
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evoked.plot()
Like the plot()
methods for :meth:Raw <mne.io.Raw.plot>
and
:meth:Epochs <mne.Epochs.plot>
objects,
:meth:evoked.plot() <mne.Evoked.plot>
has many parameters for customizing
the plot output, such as color-coding channel traces by scalp location, or
plotting the :term:global field power <GFP>
alongside the channel traces.
See tut-visualize-evoked
for more information about visualizing
:class:~mne.Evoked
objects.
Evoked
data.. sidebar:: Evokeds are not memory-mapped
:class:~mne.Evoked
objects use a :attr:~mne.Evoked.data
attribute
rather than a :meth:~mne.Epochs.get_data
method; this reflects the fact
that the data in :class:~mne.Evoked
objects are always loaded into
memory, never memory-mapped
_ from their location on disk (because they
are typically much smaller than :class:~mne.io.Raw
or
:class:~mne.Epochs
objects).
Unlike :class:~mne.io.Raw
and :class:~mne.Epochs
objects,
:class:~mne.Evoked
objects do not support selection by square-bracket
indexing. Instead, data can be subselected by indexing the
:attr:~mne.Evoked.data
attribute:
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print(evoked.data[:2, :3]) # first 2 channels, first 3 timepoints
To select based on time in seconds, the :meth:~mne.Evoked.time_as_index
method can be useful, although beware that depending on the sampling
frequency, the number of samples in a span of given duration may not always
be the same (see the time-as-index
section of the
tutorial about Raw data <tut-raw-class>
for details).
By default, when creating :class:~mne.Evoked
data from an
:class:~mne.Epochs
object, only the "data" channels will be retained:
eog
, ecg
, stim
, and misc
channel types will be dropped. You
can control which channel types are retained via the picks
parameter of
:meth:epochs.average() <mne.Epochs.average>
, by passing 'all'
to
retain all channels, or by passing a list of integers, channel names, or
channel types. See the documentation of :meth:~mne.Epochs.average
for
details.
If you've already created the :class:~mne.Evoked
object, you can use the
:meth:~mne.Evoked.pick
, :meth:~mne.Evoked.pick_channels
,
:meth:~mne.Evoked.pick_types
, and :meth:~mne.Evoked.drop_channels
methods
to modify which channels are included in an :class:~mne.Evoked
object.
You can also use :meth:~mne.Evoked.reorder_channels
for this purpose; any
channel names not provided to :meth:~mne.Evoked.reorder_channels
will be
dropped. Note that channel selection methods modify the object in-place, so
in interactive/exploratory sessions you may want to create a
:meth:~mne.Evoked.copy
first.
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evoked_eeg = evoked.copy().pick_types(meg=False, eeg=True)
print(evoked_eeg.ch_names)
new_order = ['EEG 002', 'MEG 2521', 'EEG 003']
evoked_subset = evoked.copy().reorder_channels(new_order)
print(evoked_subset.ch_names)
:class:~mne.Evoked
objects have many similarities with :class:~mne.io.Raw
and :class:~mne.Epochs
objects, including:
They can be loaded from and saved to disk in .fif
format, and their
data can be exported to a :class:NumPy array <numpy.ndarray>
(but through
the :attr:~mne.Evoked.data
attribute, not through a get_data()
method). :class:Pandas DataFrame <pandas.DataFrame>
export is also
available through the :meth:~mne.Evoked.to_data_frame
method.
You can change the name or type of a channel using
:meth:evoked.rename_channels() <mne.Evoked.rename_channels>
or
:meth:evoked.set_channel_types() <mne.Evoked.set_channel_types>
.
Both methods take :class:dictionaries <dict>
where the keys are existing
channel names, and the values are the new name (or type) for that channel.
Existing channels that are not in the dictionary will be unchanged.
:term:SSP projector <projector>
manipulation is possible through
:meth:~mne.Evoked.add_proj
, :meth:~mne.Evoked.del_proj
, and
:meth:~mne.Evoked.plot_projs_topomap
methods, and the
:attr:~mne.Evoked.proj
attribute. See tut-artifact-ssp
for more
information on SSP.
Like :class:~mne.io.Raw
and :class:~mne.Epochs
objects,
:class:~mne.Evoked
objects have :meth:~mne.Evoked.copy
,
:meth:~mne.Evoked.crop
, :meth:~mne.Evoked.time_as_index
,
:meth:~mne.Evoked.filter
, and :meth:~mne.Evoked.resample
methods.
Like :class:~mne.io.Raw
and :class:~mne.Epochs
objects,
:class:~mne.Evoked
objects have evoked.times
,
:attr:evoked.ch_names <mne.Evoked.ch_names>
, and :class:info <mne.Info>
attributes.
Evoked
dataSingle :class:~mne.Evoked
objects can be saved to disk with the
:meth:evoked.save() <mne.Evoked.save>
method. One difference between
:class:~mne.Evoked
objects and the other data structures is that multiple
:class:~mne.Evoked
objects can be saved into a single .fif
file, using
:func:mne.write_evokeds
. The example data <sample-dataset>
includes just such a .fif
file: the data have already been epoched and
averaged, and the file contains separate :class:~mne.Evoked
objects for
each experimental condition:
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sample_data_evk_file = os.path.join(sample_data_folder, 'MEG', 'sample',
'sample_audvis-ave.fif')
evokeds_list = mne.read_evokeds(sample_data_evk_file, verbose=False)
print(evokeds_list)
print(type(evokeds_list))
Notice that :func:mne.read_evokeds
returned a :class:list
of
:class:~mne.Evoked
objects, and each one has an evoked.comment
attribute describing the experimental condition that was averaged to
generate the estimate:
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for evok in evokeds_list:
print(evok.comment)
If you want to load only some of the conditions present in a .fif
file,
:func:~mne.read_evokeds
has a condition
parameter, which takes either a
string (matched against the comment attribute of the evoked objects on disk),
or an integer selecting the :class:~mne.Evoked
object based on the order
it's stored in the file. Passing lists of integers or strings is also
possible. If only one object is selected, the :class:~mne.Evoked
object
will be returned directly (rather than a length-one list containing it):
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right_vis = mne.read_evokeds(sample_data_evk_file, condition='Right visual')
print(right_vis)
print(type(right_vis))
Above, when we created an :class:~mne.Evoked
object by averaging epochs,
baseline correction was applied by default when we extracted epochs from the
class:~mne.io.Raw
object (the default baseline period is (None, 0)
,
which assured zero mean for times before the stimulus event). In contrast, if
we plot the first :class:~mne.Evoked
object in the list that was loaded
from disk, we'll see that the data have not been baseline-corrected:
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evokeds_list[0].plot(picks='eeg')
This can be remedied by either passing a baseline
parameter to
:func:mne.read_evokeds
, or by applying baseline correction after loading,
as shown here:
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evokeds_list[0].apply_baseline((None, 0))
evokeds_list[0].plot(picks='eeg')
Notice that :meth:~mne.Evoked.apply_baseline
operated in-place. Similarly,
:class:~mne.Evoked
objects may have been saved to disk with or without
:term:projectors <projector>
applied; you can pass proj=True
to the
:func:~mne.read_evokeds
function, or use the :meth:~mne.Evoked.apply_proj
method after loading.
Evoked
objectsOne way to pool data across multiple conditions when estimating evoked
responses is to do so prior to averaging (recall that MNE-Python can select
based on partial matching of /
-separated epoch labels; see
tut-section-subselect-epochs
for more info):
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left_right_aud = epochs['auditory'].average()
print(left_right_aud)
This approach will weight each epoch equally and create a single
:class:~mne.Evoked
object. Notice that the printed representation includes
(average, N=145)
, indicating that the :class:~mne.Evoked
object was
created by averaging across 145 epochs. In this case, the event types were
fairly close in number:
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left_aud = epochs['auditory/left'].average()
right_aud = epochs['auditory/right'].average()
print([evok.nave for evok in (left_aud, right_aud)])
However, this may not always be the case; if for statistical reasons it is
important to average the same number of epochs from different conditions,
you can use :meth:~mne.Epochs.equalize_event_counts
prior to averaging.
Another approach to pooling across conditions is to create separate
:class:~mne.Evoked
objects for each condition, and combine them afterward.
This can be accomplished by the function :func:mne.combine_evoked
, which
computes a weighted sum of the :class:~mne.Evoked
objects given to it. The
weights can be manually specified as a list or array of float values, or can
be specified using the keyword 'equal'
(weight each ~mne.Evoked
object
by $\frac{1}{N}$, where $N$ is the number of ~mne.Evoked
objects given) or the keyword 'nave'
(weight each ~mne.Evoked
object
proportional to the number of epochs averaged together to create it):
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left_right_aud = mne.combine_evoked([left_aud, right_aud], weights='nave')
assert left_right_aud.nave == left_aud.nave + right_aud.nave
Note that the nave
attribute of the resulting ~mne.Evoked
object will
reflect the effective number of averages, and depends on both the nave
attributes of the contributing ~mne.Evoked
objects and the weights at
which they are combined. Keeping track of effective nave
is important for
inverse imaging, because nave
is used to scale the noise covariance
estimate (which in turn affects the magnitude of estimated source activity).
See minimum_norm_estimates
for more information (especially the
whitening_and_scaling
section). Note that mne.grand_average
does
not adjust nave
to reflect effective number of averaged epochs; rather
it simply sets nave
to the number of evokeds that were averaged
together. For this reason, it is best to use mne.combine_evoked
rather than
mne.grand_average
if you intend to perform inverse imaging on the resulting
:class:~mne.Evoked
object.
Evoked
objectsAlthough the most common use of :class:~mne.Evoked
objects is to store
averages of epoched data, there are a couple other uses worth noting here.
First, the method :meth:epochs.standard_error() <mne.Epochs.standard_error>
will create an :class:~mne.Evoked
object (just like
:meth:epochs.average() <mne.Epochs.average>
does), but the data in the
:class:~mne.Evoked
object will be the standard error across epochs instead
of the average. To indicate this difference, :class:~mne.Evoked
objects
have a :attr:~mne.Evoked.kind
attribute that takes values 'average'
or
'standard error'
as appropriate.
Another use of :class:~mne.Evoked
objects is to represent a single trial
or epoch of data, usually when looping through epochs. This can be easily
accomplished with the :meth:epochs.iter_evoked() <mne.Epochs.iter_evoked>
method, and can be useful for applications where you want to do something
that is only possible for :class:~mne.Evoked
objects. For example, here
we use the :meth:~mne.Evoked.get_peak
method (which isn't available for
:class:~mne.Epochs
objects) to get the peak response in each trial:
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for ix, trial in enumerate(epochs[:3].iter_evoked()):
channel, latency, value = trial.get_peak(ch_type='eeg',
return_amplitude=True)
latency = int(round(latency * 1e3)) # convert to milliseconds
value = int(round(value * 1e6)) # convert to µV
print('Trial {}: peak of {} µV at {} ms in channel {}'
.format(ix, value, latency, channel))
.. REFERENCES