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SETUP





In [1]:



    


import time
import numpy as np
import matplotlib.pyplot as plt
import pandas as pd
import os
from config import utils as ut
%matplotlib inline

Autosipper





In [2]:



    


# config directory must have "__init__.py" file
# from the 'config' directory, import the following classes:
from config import Motor, ASI_Controller, Autosipper
autosipper = Autosipper(Motor('config/motor.yaml'), ASI_Controller('config/asi_controller.yaml'))
autosipper.coord_frames



    


















    






Initializing stage...










    
Out[2]:










  
    

      
      hardware
      deck
    

  
  
    

      transform
      [[1.0, 0.0, 0.0], [0.0, 1.0, 0.0], [0.0, 0.0, ...
      [[-1, 0, 0], [0, 1, 0], [0, 0, -1], [0, 0, 93]]
    

    

      position_table
      None
      None
    

  




















In [4]:



    


from config import gui
gui.stage_control(autosipper.XY, autosipper.Z)



    


















    






(0.0,)

















In [5]:



    


platemap = ut.generate_position_table((8,12),(9,9),95.0)
platemap['x'] = -platemap['x'] - 1.8792
platemap['y'] = platemap['y'] + 32.45
platemap.loc[platemap.shape[0]] = [96, 99, 99, 99, 'W01', -8.2492, 1.1709, 68.3999]
platemap.loc[platemap.shape[0]] = [97, 99, 99, 99, 'W02', -36.9737, 1.1709, 68.3999]

platemap['contents'] = ["" for i in range(len(platemap['name']))]
for i in range(10):
    platemap['contents'].iloc[36+i] = "conc"+str(i)

autosipper.coord_frames.hardware.position_table = platemap
platemap



    


















    
Out[5]:










  
    

      
      n
      s
      r
      c
      name
      x
      y
      z
      contents
    

  
  
    

      0
      0
      0
      0
      0
      A01
      -1.8792
      32.4500
      95.0000
      
    

    

      1
      1
      1
      0
      1
      A02
      -10.8792
      32.4500
      95.0000
      
    

    

      2
      2
      2
      0
      2
      A03
      -19.8792
      32.4500
      95.0000
      
    

    

      3
      3
      3
      0
      3
      A04
      -28.8792
      32.4500
      95.0000
      
    

    

      4
      4
      4
      0
      4
      A05
      -37.8792
      32.4500
      95.0000
      
    

    

      5
      5
      5
      0
      5
      A06
      -46.8792
      32.4500
      95.0000
      
    

    

      6
      6
      6
      0
      6
      A07
      -55.8792
      32.4500
      95.0000
      
    

    

      7
      7
      7
      0
      7
      A08
      -64.8792
      32.4500
      95.0000
      
    

    

      8
      8
      8
      0
      8
      A09
      -73.8792
      32.4500
      95.0000
      
    

    

      9
      9
      9
      0
      9
      A10
      -82.8792
      32.4500
      95.0000
      
    

    

      10
      10
      10
      0
      10
      A11
      -91.8792
      32.4500
      95.0000
      
    

    

      11
      11
      11
      0
      11
      A12
      -100.8792
      32.4500
      95.0000
      
    

    

      12
      12
      23
      1
      0
      B01
      -1.8792
      41.4500
      95.0000
      
    

    

      13
      13
      22
      1
      1
      B02
      -10.8792
      41.4500
      95.0000
      
    

    

      14
      14
      21
      1
      2
      B03
      -19.8792
      41.4500
      95.0000
      
    

    

      15
      15
      20
      1
      3
      B04
      -28.8792
      41.4500
      95.0000
      
    

    

      16
      16
      19
      1
      4
      B05
      -37.8792
      41.4500
      95.0000
      
    

    

      17
      17
      18
      1
      5
      B06
      -46.8792
      41.4500
      95.0000
      
    

    

      18
      18
      17
      1
      6
      B07
      -55.8792
      41.4500
      95.0000
      
    

    

      19
      19
      16
      1
      7
      B08
      -64.8792
      41.4500
      95.0000
      
    

    

      20
      20
      15
      1
      8
      B09
      -73.8792
      41.4500
      95.0000
      
    

    

      21
      21
      14
      1
      9
      B10
      -82.8792
      41.4500
      95.0000
      
    

    

      22
      22
      13
      1
      10
      B11
      -91.8792
      41.4500
      95.0000
      
    

    

      23
      23
      12
      1
      11
      B12
      -100.8792
      41.4500
      95.0000
      
    

    

      24
      24
      24
      2
      0
      C01
      -1.8792
      50.4500
      95.0000
      
    

    

      25
      25
      25
      2
      1
      C02
      -10.8792
      50.4500
      95.0000
      
    

    

      26
      26
      26
      2
      2
      C03
      -19.8792
      50.4500
      95.0000
      
    

    

      27
      27
      27
      2
      3
      C04
      -28.8792
      50.4500
      95.0000
      
    

    

      28
      28
      28
      2
      4
      C05
      -37.8792
      50.4500
      95.0000
      
    

    

      29
      29
      29
      2
      5
      C06
      -46.8792
      50.4500
      95.0000
      
    

    

      ...
      ...
      ...
      ...
      ...
      ...
      ...
      ...
      ...
      ...
    

    

      68
      68
      63
      5
      8
      F09
      -73.8792
      77.4500
      95.0000
      
    

    

      69
      69
      62
      5
      9
      F10
      -82.8792
      77.4500
      95.0000
      
    

    

      70
      70
      61
      5
      10
      F11
      -91.8792
      77.4500
      95.0000
      
    

    

      71
      71
      60
      5
      11
      F12
      -100.8792
      77.4500
      95.0000
      
    

    

      72
      72
      72
      6
      0
      G01
      -1.8792
      86.4500
      95.0000
      
    

    

      73
      73
      73
      6
      1
      G02
      -10.8792
      86.4500
      95.0000
      
    

    

      74
      74
      74
      6
      2
      G03
      -19.8792
      86.4500
      95.0000
      
    

    

      75
      75
      75
      6
      3
      G04
      -28.8792
      86.4500
      95.0000
      
    

    

      76
      76
      76
      6
      4
      G05
      -37.8792
      86.4500
      95.0000
      
    

    

      77
      77
      77
      6
      5
      G06
      -46.8792
      86.4500
      95.0000
      
    

    

      78
      78
      78
      6
      6
      G07
      -55.8792
      86.4500
      95.0000
      
    

    

      79
      79
      79
      6
      7
      G08
      -64.8792
      86.4500
      95.0000
      
    

    

      80
      80
      80
      6
      8
      G09
      -73.8792
      86.4500
      95.0000
      
    

    

      81
      81
      81
      6
      9
      G10
      -82.8792
      86.4500
      95.0000
      
    

    

      82
      82
      82
      6
      10
      G11
      -91.8792
      86.4500
      95.0000
      
    

    

      83
      83
      83
      6
      11
      G12
      -100.8792
      86.4500
      95.0000
      
    

    

      84
      84
      95
      7
      0
      H01
      -1.8792
      95.4500
      95.0000
      
    

    

      85
      85
      94
      7
      1
      H02
      -10.8792
      95.4500
      95.0000
      
    

    

      86
      86
      93
      7
      2
      H03
      -19.8792
      95.4500
      95.0000
      
    

    

      87
      87
      92
      7
      3
      H04
      -28.8792
      95.4500
      95.0000
      
    

    

      88
      88
      91
      7
      4
      H05
      -37.8792
      95.4500
      95.0000
      
    

    

      89
      89
      90
      7
      5
      H06
      -46.8792
      95.4500
      95.0000
      
    

    

      90
      90
      89
      7
      6
      H07
      -55.8792
      95.4500
      95.0000
      
    

    

      91
      91
      88
      7
      7
      H08
      -64.8792
      95.4500
      95.0000
      
    

    

      92
      92
      87
      7
      8
      H09
      -73.8792
      95.4500
      95.0000
      
    

    

      93
      93
      86
      7
      9
      H10
      -82.8792
      95.4500
      95.0000
      
    

    

      94
      94
      85
      7
      10
      H11
      -91.8792
      95.4500
      95.0000
      
    

    

      95
      95
      84
      7
      11
      H12
      -100.8792
      95.4500
      95.0000
      
    

    

      96
      96
      99
      99
      99
      W01
      -8.2492
      1.1709
      68.3999
      
    

    

      97
      97
      99
      99
      99
      W02
      -36.9737
      1.1709
      68.3999
      
    

  



98 rows × 9 columns



















In [8]:



    


autosipper.go_to('hardware', 'name', 'B01')

Manifold





In [9]:



    


from config import Manifold
from config.gui import manifold_control

manifold = Manifold('192.168.1.3', 'config/valvemaps/valvemap.csv', 512)
manifold.valvemap[manifold.valvemap.name>0]



    


















    
Out[9]:










  
    

      
      valve
      name
    

  
  
    

      0
      0
      inlet_out
    

    

      2
      2
      inlet_in
    

    

      8
      8
      chip_out
    

    

      9
      9
      N
    

    

      10
      10
      B1
    

    

      11
      11
      S1
    

    

      12
      12
      S2
    

    

      13
      13
      B2
    

    

      14
      14
      chip_in
    

    

      16
      16
      Waste_2
    

    

      17
      17
      bBSA_2
    

    

      18
      18
      NA_2
    

    

      19
      19
      antibody_2
    

    

      20
      20
      Extra1_2
    

    

      21
      21
      Extra2_2
    

    

      22
      22
      Protein_2
    

    

      23
      23
      Wash_2
    

    

      24
      24
      Out_2
    

    

      25
      25
      Neck_2
    

    

      26
      26
      Button2_2
    

    

      27
      27
      Sandwich2_2
    

    

      28
      28
      Sandwich1_2
    

    

      29
      29
      Button1_2
    

    

      30
      30
      In_2
    

    

      32
      32
      Waste_3
    

    

      33
      33
      bBSA_3
    

    

      34
      34
      NA_3
    

    

      35
      35
      antibody_3
    

    

      36
      36
      Extra1_3
    

    

      37
      37
      Extra2_3
    

    

      38
      38
      Protein_3
    

    

      39
      39
      Wash_3
    

    

      40
      40
      Out_3
    

    

      41
      41
      Neck_3
    

    

      42
      42
      Button2_3
    

    

      43
      43
      Sandwich2_3
    

    

      44
      44
      Sandwich1_3
    

    

      45
      45
      Button1_3
    

    

      46
      46
      In_3
    

  




















In [10]:



    


manifold_control(manifold)



    











In [ ]:



    


def valve_states():
    tmp = []
    for i in [2,0,14,8]:
        status = 'x'
        if manifold.read_valve(i):
            status = 'o'
        tmp.append([status, manifold.valvemap.name.iloc[i]])
    return pd.DataFrame(tmp)



    











In [ ]:



    


tmp = []
for i in range(16):
    status = 'x'
    if manifold.read_valve(i):
        status = 'o'
    name = manifold.valvemap.name.iloc[i]
    tmp.append([status, name])
pd.DataFrame(tmp).replace(np.nan, '')



    











In [ ]:



    


name = 'inlet_in'
v = manifold.valvemap['valve'][manifold.valvemap.name==name]



    











In [ ]:



    


v=14



    











In [ ]:



    


manifold.depressurize(v)



    











In [ ]:



    


manifold.pressurize(v)



    











In [ ]:



    


manifold.exit()

Micromanager





In [11]:



    


# !!!! Also must have MM folder on system PATH
# mm_version = 'C:\Micro-Manager-1.4'
# cfg = 'C:\Micro-Manager-1.4\SetupNumber2_01282017.cfg'
mm_version = 'C:\Program Files\Micro-Manager-2.0beta'
cfg = 'C:\Program Files\Micro-Manager-2.0beta\Setup2_20170413.cfg'

import sys
sys.path.insert(0, mm_version) # make it so python can find MMCorePy
import MMCorePy

from PIL import Image

core = MMCorePy.CMMCore()
core.loadSystemConfiguration(cfg)
core.setProperty("Spectra", "White_Enable", "1")
core.waitForDevice("Spectra")

core.setProperty("Cam Andor_Zyla4.2", "Sensitivity/DynamicRange", "16-bit (low noise & high well capacity)") # NEED TO SET CAMERA TO 16 BIT (ceiling 12 BIT = 4096)

Preset: 1_PBP
ConfigGroup,Channel,1_PBP,TIFilterBlock1,Label,1-PBP

Preset: 2_BF
ConfigGroup,Channel,2_BF,TIFilterBlock1,Label,2-BF

Preset: 3_DAPI
ConfigGroup,Channel,3_DAPI,TIFilterBlock1,Label,3-DAPI

Preset: 4_eGFP
ConfigGroup,Channel,4_eGFP,TIFilterBlock1,Label,4-GFP

Preset: 5_Cy5
ConfigGroup,Channel,5_Cy5,TIFilterBlock1,Label,5-Cy5

Preset: 6_AttoPhos
ConfigGroup,Channel,6_AttoPhos,TIFilterBlock1,Label,6-AttoPhos

ACQUISITION





In [ ]:



    


core.setProperty(core.getCameraDevice(), "Exposure", 125)
core.setConfig('Channel','4_eGFP')
core.setProperty(core.getCameraDevice(), "Binning", "3x3")



    











In [ ]:



    


position_list = ut.load_mm_positionlist("Z:/Data/Setup 2/Arjun/170609_FlippedMITOMI/170609_mwm.pos")
position_list



    











In [ ]:



    


# ONE ACQUISITION / SCAN
def scan(channel, exposure, washtype, plate_n):
    core.setConfig('Channel', channel)
    core.setProperty(core.getCameraDevice(), "Exposure", exposure)
    time.sleep(.2)
    
    timestamp = time.strftime("%Y%m%d-%H%M%S", time.localtime())
    rootdirectory = "Z:/Data/Setup 2/Arjun/170609_FlippedMITOMI/"
    solution = autosipper.coord_frames.hardware.position_table.contents.iloc[plate_n]
    scandirectory = '{}_{}_{}_{}_{}'.format(timestamp, solution, washtype, channel, exposure)
    os.makedirs(rootdirectory+scandirectory)
    
    for i in xrange(len(position_list)):
        si = str(i)
        x,y = position_list[['x','y']].iloc[i]
        core.setXYPosition(x,y)
        core.waitForDevice(core.getXYStageDevice())
        
        core.snapImage()
        img = core.getImage()
        image = Image.fromarray(img)
        
        timestamp = time.strftime("%Y%m%d-%H%M%S", time.localtime())
        positionname = position_list['name'].iloc[i]
        image.save('{}/{}_{}.tif'.format(rootdirectory+scandirectory, timestamp, positionname))

    x,y = position_list[['x','y']].iloc[0]
    core.setXYPosition(x,y)
    core.waitForDevice(core.getXYStageDevice())

def get_valve(name):
    return ut.lookup(manifold.valvemap,'name',name,'valve',0)[0]



    











In [ ]:



    


scan('4_eGFP', 125, 'pre')



    











In [ ]:



    


# PUT PINS IN ALL INPUTS
# in0 WASTE LINE
# in1 WASH LINE, 4.5 PSI
# in2 PEEK LINE, 4.5 PSI
#----------------------------------

# initialize valve states
## all inputs close
## sandwhich

# prime inlet tree (wash)
manifold.depressurize(get_valve('bBSA_2'))  ## wash open
manifold.depressurize(get_valve('Waste_2')) ## waste open
time.sleep(60*0.5)                          ## wait 0.5 min
manifold.pressurize(get_valve('Waste_2'))   ## waste close

# backflush tubing (wash)
autosipper.go_to('hardware', 'name', 'W02', zh_travel=40) ## W02 move
manifold.depressurize(get_valve('NA_2'))                  ## inlet open
time.sleep(60*11)                                         ## wait 11 min
manifold.pressurize(get_valve('NA_2'))                    ## inlet close

# fill chip (wash)
## chip_in open
## chip_out open
## wait 10 min
## chip_out close
## wait 10 min

# prime tubing (1st input)
manifold.pressurize(get_valve('Out_2'))                   # chip_out close
manifold.pressurize(get_valve('In_2'))                    # chip_in close

autosipper.go_to('hardware', 'name', 'W01', zh_travel=40)
autosipper.go_to('hardware', 'n', 36, zh_travel=40)
manifold.depressurize(get_valve('NA_2'))                  # inlet open
manifold.depressurize(get_valve('Waste_2'))               # waste open
time.sleep(60*11)                                         # filling inlet, 11 min...

manifold.pressurize(get_valve('Waste_2'))                 # waste close
  • first attempt above: forgot to close wash after backflush




In [ ]:



    


# prime inlet tree (wash)
manifold.depressurize(get_valve('bBSA_2'))  ## wash open
manifold.depressurize(get_valve('Waste_2')) ## waste open
time.sleep(60*0.5)                          ## wait 0.5 min
manifold.pressurize(get_valve('Waste_2'))   ## waste close

# backflush tubing (wash)
autosipper.go_to('hardware', 'name', 'W02', zh_travel=40) ## W02 move
manifold.depressurize(get_valve('NA_2'))                  ## inlet open
time.sleep(60*15)                                         ## wait 15 min
manifold.pressurize(get_valve('NA_2'))                    ## inlet close
manifold.pressurize(get_valve('bBSA_2'))                  ## wash close

# prime tubing (1st input)
manifold.pressurize(get_valve('Out_2'))                   # chip_out close
manifold.pressurize(get_valve('In_2'))                    # chip_in close

autosipper.go_to('hardware', 'name', 'W01', zh_travel=40)
autosipper.go_to('hardware', 'n', 36, zh_travel=40)
manifold.depressurize(get_valve('NA_2'))                  # inlet open
manifold.depressurize(get_valve('Waste_2'))               # waste open
time.sleep(60*15)                                         # filling inlet, 15 min...

manifold.pressurize(get_valve('Waste_2'))                 # waste close



    











In [ ]:



    


# 16,Waste_2
# 17,bBSA_2
# 18,NA_2
# 19,antibody_2
# 20,Extra1_2
# 21,Extra2_2
# 22,Protein_2
# 23,Wash_2

exposures = [1250,1250,1250,1250,1000,600,300,180,70,30]
for i,exposure in enumerate(exposures):
    plate_n = 36+i
    
    # flow on chip
    manifold.depressurize(get_valve('NA_2')) # inlet open
    manifold.depressurize(get_valve('In_2')) # chip_in open
    manifold.depressurize(get_valve('Out_2')) # chip_out open
    time.sleep(60*10) # filling chip, 10 min...
    
    # CONCURRENTLY:
    incubate_time = 60*30 # 30 min
    
    # a) incubate DNA with protein
    manifold.pressurize(get_valve('Sandwich1_2')) # sandwhiches close
    manifold.pressurize(get_valve('Sandwich2_2')) # "
    time.sleep(1) # pause
    manifold.depressurize(get_valve('Button1_2')) # buttons open
    manifold.depressurize(get_valve('Button2_2')) # "
    incubate_start = time.time()
    
    # b1) prime inlet tube with next INPUT
    manifold.pressurize(get_valve('Out_2')) # chip_out close
    manifold.pressurize(get_valve('In_2')) # chip_in close

    manifold.pressurize(get_valve('NA_2')) # inlet close
    autosipper.go_to('hardware', 'name', 'W01', zh_travel=40)
    autosipper.go_to('hardware', 'n', plate_n+1, zh_travel=40)
    manifold.depressurize(get_valve('NA_2')) # inlet open
    manifold.depressurize(get_valve('Waste_2')) # waste open
    time.sleep(60*15) # filling inlet, 15 min...
    
    manifold.pressurize(get_valve('NA_2')) # inlet close

    # b2) prime inlet tree with wash
    manifold.depressurize(get_valve('bBSA_2')) # wash open
    manifold.pressurize(get_valve('Waste_2')) # waste close
    
    for v in [19,20,21,22,23]:
        manifold.depressurize(v)
        time.sleep(.2)
        manifold.pressurize(v)
    
    manifold.depressurize(get_valve('Waste_2')) # waste open
    time.sleep(60*1)
    manifold.pressurize(get_valve('Waste_2')) # waste close

    remaining_time = incubate_time - (time.time() - incubate_start)
    time.sleep(remaining_time)    
    
    # prewash Cy5
    scan('5_Cy5', exposure, 'pre', plate_n)
    
    # wash
    manifold.pressurize(get_valve('Button1_2')) # buttons close
    manifold.pressurize(get_valve('Button2_2')) # "
    time.sleep(1) # pause
    manifold.depressurize(get_valve('Sandwich1_2')) # sandwhiches open
    manifold.depressurize(get_valve('Sandwich2_2')) # "
        
    manifold.depressurize(get_valve('In_2')) # chip_in open
    manifold.depressurize(get_valve('Out_2')) # chip_out open
    time.sleep(60*10) # washing chip, 10 min...
    
    manifold.pressurize(get_valve('Out_2')) # chip_out close
    manifold.pressurize(get_valve('In_2')) # chip_in close
    manifold.pressurize(get_valve('bBSA_2')) # wash close

    # postwash eGFP and postwash Cy5
    scan('4_eGFP', 125, 'post', plate_n)
    scan('5_Cy5', 1250, 'post', plate_n)



    











In [ ]:



    


autosipper.go_to('hardware', 'n', 47, zh_travel=40)



    











In [ ]:



    


# prewash Cy5
scan('5_Cy5', exposure, 'pre', plate_n)

# wash
manifold.pressurize(get_valve('Button1_2')) # buttons close
manifold.pressurize(get_valve('Button2_2')) # "
time.sleep(1) # pause
manifold.depressurize(get_valve('Sandwich1_2')) # sandwhiches open
manifold.depressurize(get_valve('Sandwich2_2')) # "

manifold.depressurize(get_valve('In_2')) # chip_in open
manifold.depressurize(get_valve('Out_2')) # chip_out open
time.sleep(60*10) # washing chip, 10 min...

manifold.pressurize(get_valve('Out_2')) # chip_out close
manifold.pressurize(get_valve('In_2')) # chip_in close
manifold.pressurize(get_valve('bBSA_2')) # wash close

# postwash eGFP and postwash Cy5
scan('4_eGFP', 125, 'post', plate_n)
scan('5_Cy5', 1250, 'post', plate_n)


exposures = [1250,1250,1250,1250,1000,600,300,180,70,30]
for i, exposure in enumerate(exposures):
    if i==0:
        continue
    
    plate_n = 36+i
    
    # flow on chip
    manifold.depressurize(get_valve('NA_2')) # inlet open
    manifold.depressurize(get_valve('In_2')) # chip_in open
    manifold.depressurize(get_valve('Out_2')) # chip_out open
    time.sleep(60*10) # filling chip, 10 min...
    
    # CONCURRENTLY:
    incubate_time = 60*30 # 30 min
    
    # a) incubate DNA with protein
    manifold.pressurize(get_valve('Sandwich1_2')) # sandwhiches close
    manifold.pressurize(get_valve('Sandwich2_2')) # "
    time.sleep(1) # pause
    manifold.depressurize(get_valve('Button1_2')) # buttons open
    manifold.depressurize(get_valve('Button2_2')) # "
    incubate_start = time.time()
    
    # b1) prime inlet tube with next INPUT
    manifold.pressurize(get_valve('Out_2')) # chip_out close
    manifold.pressurize(get_valve('In_2')) # chip_in close

    manifold.pressurize(get_valve('NA_2')) # inlet close
    autosipper.go_to('hardware', 'name', 'W01', zh_travel=40)
    autosipper.go_to('hardware', 'n', plate_n+1, zh_travel=40)
    manifold.depressurize(get_valve('NA_2')) # inlet open
    manifold.depressurize(get_valve('Waste_2')) # waste open
    time.sleep(60*15) # filling inlet, 15 min...
    
    manifold.pressurize(get_valve('NA_2')) # inlet close

    # b2) prime inlet tree with wash
    manifold.depressurize(get_valve('bBSA_2')) # wash open
    manifold.pressurize(get_valve('Waste_2')) # waste close
    
    for v in [19,20,21,22,23]:
        manifold.depressurize(v)
        time.sleep(.2)
        manifold.pressurize(v)
    
    manifold.depressurize(get_valve('Waste_2')) # waste open
    time.sleep(60*1)
    manifold.pressurize(get_valve('Waste_2')) # waste close

    remaining_time = incubate_time - (time.time() - incubate_start)
    time.sleep(remaining_time)    
    
    # prewash Cy5
    scan('5_Cy5', exposure, 'pre', plate_n)
    
    # wash
    manifold.pressurize(get_valve('Button1_2')) # buttons close
    manifold.pressurize(get_valve('Button2_2')) # "
    time.sleep(1) # pause
    manifold.depressurize(get_valve('Sandwich1_2')) # sandwhiches open
    manifold.depressurize(get_valve('Sandwich2_2')) # "
        
    manifold.depressurize(get_valve('In_2')) # chip_in open
    manifold.depressurize(get_valve('Out_2')) # chip_out open
    time.sleep(60*10) # washing chip, 10 min...
    
    manifold.pressurize(get_valve('Out_2')) # chip_out close
    manifold.pressurize(get_valve('In_2')) # chip_in close
    manifold.pressurize(get_valve('bBSA_2')) # wash close

    # postwash eGFP and postwash Cy5
    scan('4_eGFP', 125, 'post', plate_n)
    scan('5_Cy5', 1250, 'post', plate_n)

EXIT





In [ ]:



    


autosipper.exit()
manifold.exit()
core.unloadAllDevices()
core.reset()
print 'closed'

Content source: FordyceLab/AcqPack

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