pYPKp7

The pYPKp7 is a version of the pYPK7 with a deletion in the crp gene that originates from the pCAPs vector.



In [1]:

    
from pydna.all import *

pYPK7 =read("pYPK7.gb")



In [2]:

    
f,r =parse(''' >866_pYPKpwF (79-mer)
                      GCATGATATCttcacaggcggttttcgcacgtacccatg

                      >865_pYPKpwR (79-mer)
                      GCATGACGTCaccagacgctatgactcacccggacggca''', ds=False)

seq =pcr(f, r, pYPK7)

seq = seq.looped()

pYPKp7 = seq.synced(pYPK7)

pYPKp7.stamp()









    Out[2]:





cSEGUID_w8zCE05nnVaKMSe-K0q29kjVt4k



In [3]:

    
pYPKp7.locus = "pYPKp7"

pYPKp7.write("pYPKp7.gb")









    




pYPKp7.gb

DOWNLOAD



In [4]:

    
from pydna.all import *
reloaded=read("pYPKp7.gb")
assert reloaded.cseguid() in reloaded.definition



In [5]: