pYPKp3

The pYPKp3 is a version of the pYPK3 with a deletion in the crp gene that originates from the pCAPs vector.


In [1]:
from pydna.all import *

pYPK3 =read("pYPK3.gb")

In [2]:
f,r =parse(''' >866_pYPKpwF (39-mer)
                      GCATGATATCttcacaggcggttttcgcacgtacccatg

                      >865_pYPKpwR (39-mer)
                      GCATGACGTCaccagacgctatgactcacccggacggca''', ds=False)

seq =pcr(f, r, pYPK3)

seq = seq.looped()

pYPKp3 = seq.synced(pYPK3)

In [3]:
pYPKp3.stamp()


Out[3]:
cSEGUID_K5LiPVZQhbNWgHgs6lyVJaKgmas

In [4]:
pYPKp3.locus = "pYPKp3"

In [5]:
pYPKp3.write("pYPKp3.gb")





In [6]:
from pydna.all import *
reloaded=read("pYPKp3.gb")
assert reloaded.cseguid() in reloaded.definition