In [1]:
from pydna.all import *
pYPK3 =read("pYPK3.gb")
In [2]:
f,r =parse(''' >866_pYPKpwF (39-mer)
GCATGATATCttcacaggcggttttcgcacgtacccatg
>865_pYPKpwR (39-mer)
GCATGACGTCaccagacgctatgactcacccggacggca''', ds=False)
seq =pcr(f, r, pYPK3)
seq = seq.looped()
pYPKp3 = seq.synced(pYPK3)
In [3]:
pYPKp3.stamp()
Out[3]:
In [4]:
pYPKp3.locus = "pYPKp3"
In [5]:
pYPKp3.write("pYPKp3.gb")
In [6]:
from pydna.all import *
reloaded=read("pYPKp3.gb")
assert reloaded.cseguid() in reloaded.definition