pYPKp3

The pYPKp3 is a version of the pYPK3 with a deletion in the crp gene that originates from the pCAPs vector.



In [1]:

    
from pydna.all import *

pYPK3 =read("pYPK3.gb")



In [2]:

    
f,r =parse(''' >866_pYPKpwF (39-mer)
                      GCATGATATCttcacaggcggttttcgcacgtacccatg

                      >865_pYPKpwR (39-mer)
                      GCATGACGTCaccagacgctatgactcacccggacggca''', ds=False)

seq =pcr(f, r, pYPK3)

seq = seq.looped()

pYPKp3 = seq.synced(pYPK3)



In [3]:

    
pYPKp3.stamp()









    Out[3]:





cSEGUID_K5LiPVZQhbNWgHgs6lyVJaKgmas



In [4]:

    
pYPKp3.locus = "pYPKp3"



In [5]:

    
pYPKp3.write("pYPKp3.gb")









    




pYPKp3.gb

DOWNLOAD



In [6]:

    
from pydna.all import *
reloaded=read("pYPKp3.gb")
assert reloaded.cseguid() in reloaded.definition