In [1]:
from pydna.all import *
pYPK4 =read("pYPK4.gb")
In [2]:
f,r =parse(''' >866_pYPKpwF (49-mer)
GCATGATATCttcacaggcggttttcgcacgtacccatg
>865_pYPKpwR (49-mer)
GCATGACGTCaccagacgctatgactcacccggacggca''', ds=False)
seq =pcr(f, r, pYPK4)
seq = seq.looped()
pYPKp4 = seq.synced(pYPK4)
pYPKp4 = seq.synced(pYPK4)
pYPKp4.stamp()
Out[2]:
In [3]:
pYPKp4.locus = "pYPKp4"
In [4]:
pYPKp4.write("pYPKp4.gb")
In [5]:
from pydna.all import *
reloaded=read("pYPKp4.gb")
assert reloaded.cseguid() in reloaded.definition
In [6]: