pYPKp4

The pYPKp4 is a version of the pYPK4 with a deletion in the crp gene that originates from the pCAPs vector.


In [1]:
from pydna.all import *

pYPK4 =read("pYPK4.gb")

In [2]:
f,r =parse(''' >866_pYPKpwF (49-mer)
                      GCATGATATCttcacaggcggttttcgcacgtacccatg

                      >865_pYPKpwR (49-mer)
                      GCATGACGTCaccagacgctatgactcacccggacggca''', ds=False)

seq =pcr(f, r, pYPK4)

seq = seq.looped()

pYPKp4 = seq.synced(pYPK4)

pYPKp4 = seq.synced(pYPK4)

pYPKp4.stamp()


Out[2]:
cSEGUID_24-DQfaAOdXPwn9hfx-xzkNuH7Y

In [3]:
pYPKp4.locus = "pYPKp4"

In [4]:
pYPKp4.write("pYPKp4.gb")





In [5]:
from pydna.all import *
reloaded=read("pYPKp4.gb")
assert reloaded.cseguid() in reloaded.definition

In [6]: