pYPKp4

The pYPKp4 is a version of the pYPK4 with a deletion in the crp gene that originates from the pCAPs vector.



In [1]:

    
from pydna.all import *

pYPK4 =read("pYPK4.gb")



In [2]:

    
f,r =parse(''' >866_pYPKpwF (49-mer)
                      GCATGATATCttcacaggcggttttcgcacgtacccatg

                      >865_pYPKpwR (49-mer)
                      GCATGACGTCaccagacgctatgactcacccggacggca''', ds=False)

seq =pcr(f, r, pYPK4)

seq = seq.looped()

pYPKp4 = seq.synced(pYPK4)

pYPKp4 = seq.synced(pYPK4)

pYPKp4.stamp()









    Out[2]:





cSEGUID_24-DQfaAOdXPwn9hfx-xzkNuH7Y



In [3]:

    
pYPKp4.locus = "pYPKp4"



In [4]:

    
pYPKp4.write("pYPKp4.gb")









    




pYPKp4.gb

DOWNLOAD



In [5]:

    
from pydna.all import *
reloaded=read("pYPKp4.gb")
assert reloaded.cseguid() in reloaded.definition



In [6]: