Construction of the pYPK0_HIS3 vector

In [1]:

    

from pydna.all import *


    

In [2]:

    

pYPK0 = read("pYPK0.gb")


    

In [3]:

    

from Bio.Restriction import ZraI, AjiI, EcoRV, SpeI, PstI


    

In [4]:

    

pYPK0_lin = pYPK0.linearize(SpeI)


    

In [5]:

    

pYPKa_A_HIS3 =read("pYPKa_A_ScHIS3.gb")


    

In [6]:

    

p567,p577,p468,p467,p568,p578,p775,p778,p167,p166 = parse("yeast_pahtway_kit_standard_primers.txt")


    

In [7]:

    

g = pcr( p468, p467, pYPKa_A_HIS3)


    

In [8]:

    

asm =Assembly((pYPK0_lin, g))
asm


    

    
Out[8]:





Assembly
fragments..: 5766bp 761bp
limit(bp)..: 25
G.nodes....: 4
algorithm..: common_sub_strings

In [9]:

    

candididate = asm.assemble_circular()[0]


    

In [10]:

    

candididate.figure()


    

    
Out[10]:





 -|pYKP0_lin|35
|            \/
|            /\
|            35|761bp_PCR_prod|29
|                              \/
|                              /\
|                              29-
|                                 |
 ---------------------------------

In [11]:

    

final = candididate.synced(pYPK0)


    

In [12]:

    

final.write("pYPK0_HIS3.gb")


    

    





pYPK0_HIS3.gb