Xylose pathway constructs described in Pereira et al. 2015

This is an Jupyter notebook (ipynb) file. An ipynb file is document containing a mixture between Python source code and text. This file links to other ipynb files which describe in detail every genetic construction made as a part of this work (Pereira et al. 2015) using the software package pydna.

Since pydna in used, the documentation is executable on the users computer if downloaded. To execute the Jupyter notebooks, Python and pydna and its dependencies must be installed. The simplest way to acchieve this is to install the free Python distribution Anaconda for which there is a pydna package for most platforms.

Standard primers and additional information on the yeast pathway kit can be found here ypk_std_primers.

Multigene xylose metabolic pathways

Single gene expression vectors

Promoter vectors

Gene vectors

Terminator vectors

Pathway backbone vectors

HIS3 expression constructs

Miscellaneous vectors