This repository contains Jupyter notebooks and data files that demonstrate how pydna can be used to plan, document and simulate cloning experiments.
Construction of the YEp24PGK_XK vector using restriction and ligation.
Construction of the pGUP1 sequence using in-vivo homologous recombination
Assembly of the 32 Mumberg expression vectors
Assembly of the pGreenLantern-1 sequence.
Expression of the Strawberry aat gene in Saccharomyces cerevisiae using the Yeast Pathway Kit
Static versions of the notebooks can also be displayed as web pages through nbviewer.
Click on the red and black "launch binder" image above to run the notebooks on the cloud service Mybinder. This way notebooks can be executed online without having to install anything on the users computer.
N.B. Mybinder runs on infrastructure paid for by the Freeman lab. Please do not abuse the service. If you want to do extensive testing of these notebooks, it is better to download this repository and run the notebooks locally (the notebooks will also run faster).
The contents of this repository is released under the MIT Licence
Use this Digital Object Identifier (DOI) when citing this repository.