In [1]:
import matplotlib
import numpy as np
import matplotlib.pyplot as plt
from scipy import stats
from scipy import io
import scipy.io as sio
%matplotlib inline
import pylab
import csv
from Tkinter import Tk
from tkFileDialog import askopenfilename
from tkFileDialog import askdirectory
import nibabel as nb
from scipy import io
#from nifti import NiftiImage
import nibabel as nb
from scipy.interpolate import interp1d
from scipy import ndimage
In [2]:
# from http://stackoverflow.com/questions/3579568/choosing-a-file-in-python-with-simple-dialog
Tk().withdraw() # we don't want a full GUI, so keep the root window from appearing
filename = askopenfilename() # show an "Open" dialog box and return the path to the selected file
print(filename)
In [3]:
Ua=sio.loadmat(filename)
DT=Ua['TSo']
DT.shape
Out[3]:
In [4]:
# from http://stackoverflow.com/questions/3579568/choosing-a-file-in-python-with-simple-dialog
Tk().withdraw() # we don't want a full GUI, so keep the root window from appearing
filename2 = askopenfilename() # show an "Open" dialog box and return the path to the selected file
print(filename2)
In [5]:
img1 = nb.load(filename2)
data = img1.get_data()
S=data.shape
S
Out[5]:
In [6]:
S=data.shape
S
Out[6]:
Z-score
In [7]:
Demean=np.zeros(S)
Dmaps=np.zeros(S)
Dvar=np.zeros(S)
Var=np.zeros(S[3])
D2=np.zeros([S[0],S[1],5,S[3]])
Tvar=np.zeros(S[3])
In [8]:
for i in range(S[3]):
Demean[:,:,:,i]=data[:,:,:,i]-np.mean(np.mean(np.mean(data[:,:,:,i],0),0),0)
In [9]:
for i in range(S[3]):
Dsq=np.reshape(Demean[:,:,:,i],S[0]*S[1]*S[2])
Var[i]=np.sqrt(np.var(Dsq))
Dvar=Demean[:,:,:,i]/Var[i]
Dmaps[:,:,:,i]=Dvar-2.5
Tvar[i]=np.var(DT[i,:])
Dmaps[Dmaps<0]=0
In [10]:
# from http://stackoverflow.com/questions/3579568/choosing-a-file-in-python-with-simple-dialog
from Tkinter import Tk
from tkFileDialog import askopenfilename
Tk().withdraw() # we don't want a full GUI, so keep the root window from appearing
filenameM = askopenfilename() # show an "Open" dialog box and return the path to the selected file
print(filenameM)
img1 = nb.load(filenameM)
Masks = img1.get_data()
Sm=Masks.shape
Masks=np.array(Masks)
In [11]:
filenameM='/home/sophie/LargeRegionList'
with open(filenameM) as f:
content = f.readlines()
Names=[Line.replace('\n','').split(' ') for Line in content]
RegionName=[Names[i][1] for i in range(12)]
Num=[int(Names[i][0]) for i in range(12)]
In [12]:
RegionName
Out[12]:
In [13]:
Dmaps.shape
Out[13]:
In [14]:
M=np.zeros((S[3],13))
Mapmean=np.zeros(S[3])
MMasks=np.zeros(13)
In [15]:
for i in range(S[3]):
Mapmean[i]=np.mean(np.mean(np.mean(Dmaps[:,:,:,i])))
for j in range(12):
MMasks[j]=np.mean(np.mean(np.mean(Masks[:,:,:,j])))
if MMasks[j]:
M[i,j]=np.mean(np.mean(np.mean(Masks[:,:,:,j]*Dmaps[:,:,:,i])))/(MMasks[j]*Mapmean[i])
In [16]:
CompMainName=S[3]*['']
CompNameAdd=np.zeros((S[3],12))
for i in range(S[3]):
Max=np.max(M[i,:])
I=np.argmax(M[i,:])+1
for j in range(12):
J=[l for l in range(12) if Num[l]==(j+1)]
if M[i,j]>0.2*Max:
CompNameAdd[i,J]=1
J=[l for l in range(12) if Num[l]==I]
if J!= []:
CompMainName[i]=Names[np.array(J)][0]
In [17]:
J
Out[17]:
In [61]:
pylab.rcParams['figure.figsize'] = (13, 2.5)
h=5
tot=0
GoodICAnat=np.zeros(S[3])
for l in range(12):
Final_maps=np.zeros((S[0],S[1],3))
Fmap=np.zeros((S[0],S[1],3))
C=np.zeros(3)
n=0
for i in range(len(CompMainName)):
Dmmv=np.mean(data[:,:,:,i],2)
Dmmv[Dmmv<0.2*np.max(np.max(np.max(Dmmv)))]=0
C=np.squeeze(np.random.rand(3,1))
labeled, nrobject=ndimage.label(Dmmv>0)
if CompMainName[i]==Names[l][0] and (sum(CompNameAdd[i,:])<5) and nrobject<200:
n=n+1
for k in range(3):
Fmap[:,:,k]=0.7*Dmmv*C[k]/np.max(C)
Final_maps=Final_maps+Fmap
#plt.plot(Time_fluoICA.T,(DT[:,i]/np.sqrt(np.var(DT[:,i]))-h*n+2),color=C/2)
plt.plot((DT[:,i]/np.sqrt(np.var(DT[:,i]))-h*n+2),color=C/2)
tot=tot+1
GoodICAnat[i]=1
print(i)
if n!=0:
print(RegionName[l])
plt.show()
FM=Final_maps/np.max(np.max(Final_maps))
FM[FM<0.1]=0
plt.imshow(FM,interpolation='none')
plt.show()
frame1 = plt.gca()
frame1.axes.get_xaxis().set_visible(False)
frame1.axes.get_yaxis().set_visible(False)
In [62]:
BadICs=[1,2,3,4,5,6,7,8,10,11,15,16,0,19,175,115,157,159,149,158,153,21,23,24,25,29,34,37,38,39,40,41,42,43,47,48,50,55,57,59,61,64,65,69,70,73,77,78,79,82,86,89,90,92,93,94,96,97,102,104,105,113,116,128,132,138,140,22,27,30,33,46,51,87]
In [63]:
for idx in BadICs:
GoodICAnat[idx] = 0.0
In [66]:
pylab.rcParams['figure.figsize'] = (13, 3)
h=5
tot=0
NumberInLargeRegion=np.zeros(13)
for l in range(12):
Final_maps=np.zeros((S[0],S[1],3))
Fmap=np.zeros((S[0],S[1],3))
C=np.zeros(3)
n=0
for i in range(len(CompMainName)):
Dmmv=np.mean(data[:,:,:,i],2)
Dmmv[Dmmv<0.2*np.max(np.max(np.max(Dmmv)))]=0
C=np.squeeze(np.random.rand(3,1))
labeled, nrobject=ndimage.label(Dmmv>0)
if CompMainName[i]==Names[l][0] and (sum(CompNameAdd[i,:])<5) and nrobject<200 and GoodICAnat[i]==1:
n=n+1
for k in range(3):
Fmap[:,:,k]=0.7*Dmmv*(C[k]+0.2)/np.max(C+0.2)
Final_maps=Final_maps+Fmap
#plt.plot(Time_fluoICA.T,(DT[:,i]/np.sqrt(np.var(DT[:,i]))-h*n+2),color=C/2)
plt.plot((DT[:,i]/np.sqrt(np.var(DT[:,i]))-h*n+2),color=C/2)
tot=tot+1
GoodICAnat[i]=1
print(i)
if n!=0:
print(RegionName[l])
plt.show()
FM=Final_maps/np.max(np.max(Final_maps))
FM[FM<0.1]=0
plt.imshow(FM,interpolation='none')
plt.show()
frame1 = plt.gca()
frame1.axes.get_xaxis().set_visible(False)
frame1.axes.get_yaxis().set_visible(False)
NumberInLargeRegion[l]=n
In [67]:
Good_ICs
Out[67]:
In [43]:
# from http://stackoverflow.com/questions/3579568/choosing-a-file-in-python-with-simple-dialog
Tk().withdraw() # we don't want a full GUI, so keep the root window from appearing
filename = askopenfilename() # show an "Open" dialog box and return the path to the selected file
print(filename)
Ua=sio.loadmat(filename)
Xk=Ua['Xk']
In [44]:
# from http://stackoverflow.com/questions/3579568/choosing-a-file-in-python-with-simple-dialog
from Tkinter import Tk
from tkFileDialog import askopenfilename
Tk().withdraw() # we don't want a full GUI, so keep the root window from appearing
filenamet = askopenfilename() # show an "Open" dialog box and return the path to the selected file
print(filenamet)
nimt=nb.load(filenamet)
Dtemp=np.squeeze(nimt.get_data())
Dtemp.shape
Out[44]:
In [45]:
if S[2]>5:
Nstack=5
Int100=[(i+1)*100/Nstack for i in range(Nstack)]
Percs=np.percentile(range(S[2]),Int100)
Indices=np.split(range(S[2]),Percs)
D1=np.zeros([S[0],S[1],Nstack])
Dmean=np.squeeze(data[:,:,range(Nstack),2])
for i in range(Nstack):
Vmean=np.mean(Dtemp[:,:,Indices[i]],2)
Dmean[:,:,i]=Vmean
else:
Nstack=S[2]
D1=np.zeros([S[0],S[1],S[2]])
Dmean=data[:,:,range(S[2])]
Dmean=np.squeeze(Dtemp[:,:,:])
for j in range(S[3]):
a=''
if S[2]>5:
for i in range(Nstack):
V=Dmaps[:,:,Indices[i],j]
D1[:,:,i]=np.max(V,2)
D2[:,:,:,j]=D1
D1[D1==0]=np.nan
plt.imshow(Dmean[:,:,1],cmap=plt.cm.gray)
Out[45]:
In [46]:
from sklearn import linear_model
In [100]:
my_cmap=plt.cm.jet
my_cmap.set_bad(alpha=0)
Label_ICs=[]
pylab.rcParams['figure.figsize'] = (15, 2.5)
algorithm = linear_model.LinearRegression()
Sxk=Xk.shape
Sxk
X=np.zeros((Sxk[0],2))
X[:,0]=(Xk[:,0]-np.mean(Xk[:,0]))/np.std(Xk[:,0])
X[:,1]=(Xk[:,1]-np.mean(Xk[:,1]))/np.std(Xk[:,1])
#X[:,2]=(Xk[:,3]-np.mean(Xk[:,3]))/np.std(Xk[:,3])
#X[:,3]=(Xk[:,4]-np.mean(Xk[:,4]))/np.std(Xk[:,4])
#X[:,4]=(Xk[:,6]-np.mean(Xk[:,6]))/np.std(Xk[:,6])
#X[:,5]=(Xk[:,7]-np.mean(Xk[:,7]))/np.std(Xk[:,7])
plt.plot(X[:,0])
plt.plot(X[:,1])
Out[100]:
In [106]:
Rsq=np.zeros((1,S[3]))
Betas=np.zeros((2,S[3]))
X.shape
DT.shape
for j in range(S[3]):
model = algorithm.fit(X, DT[:,j])
Betas[:,j] = model.coef_
Rsq[:,j] = model.score(X,DT[:,j])
plt.plot(DT[:,88]*5)
plt.plot(X)
Out[106]:
In [102]:
RsqUni=np.zeros((6,S[3]))
BetaUni=np.zeros((6,S[3]))
Sx=X.shape
for k in range(2):
for j in range(S[3]):
model = algorithm.fit(np.reshape(X[:,k],(Sx[0],1)), DT[:,j])
BetaUni[k,j] = model.coef_
RsqUni[k,j] = model.score(np.reshape(X[:,k],(Sx[0],1)),DT[:,j])
plt.plot(Betas[0,:])
Out[102]:
In [103]:
import random
In [111]:
if S[2]>5:
Final_map=np.zeros([S[0],S[1],5,3])
Fmaps=np.zeros([S[0],S[1],5,3])
else:
Final_map=np.zeros([S[0],S[1],3])
Fmaps=np.zeros([S[0],S[1],3])
C=np.zeros([S[3],3])
C1=np.zeros([6,3])
C1[0][:]=(1,0,0)
C1[1][:]=(0,1,0)
C1[2][:]=(0,0,1)
C1[3][:]=(0.8,0.8,0)
C1[4][:]=(0,1,1)
C1[5][:]=(1,0,1)
S1=DT.shape
In [112]:
C=np.zeros((S[3],3))
i=0
l=0
Betas2=Betas
LightNuminRegion=np.zeros(12)
for j in range(S[3]):
if GoodICAnat[j] and Betas2[0,j]>0.45*np.max(Betas2[0,:]) and Betas2[1,j]<0.45*np.max(Betas2[1,:]):
#if 1>0.1:
#C[j,:]=C1[i%6][:]
C[j,2]=1
C[j,1]=Betas2[0,j]/np.max(Betas2[0,:])
#C[j,2]=1
for k in range(3):
M=np.max(np.squeeze(np.reshape(D2[:,:,:,j],S[0]*S[1]*5)))
Fmaps[:,:,:,k]=0.25*D2[:,:,:,j]*C[j,k]/M
Final_map=Final_map+Fmaps
#Betas[0,j]=0
#print(Indexo[j])
print(j+1)
print(RegionName[int(CompMainName[j])-1])
LightNuminRegion[int(CompMainName[j])-1]=LightNuminRegion[int(CompMainName[j])-1]+1
i=i+1
l=l+1
#if l==2:
#break
In [113]:
pylab.rcParams['figure.figsize'] = (15, 6)
C2=np.zeros(3)
Df=np.zeros([S[0],S[1],5,3])
for i in range(3):
Df[:,:,:,i]=Final_map[:,:,:,i]+Dmean/10
#Df=Df/(np.max(np.max(np.max(Df),3)))
if S[2]>5:
N=Nstack
else:
N=S[2]
for i in range(N):
#if Good_ICs[j]:
plt.subplot(1,N,i+1)
plt.imshow(Df[:,:,i],cmap=plt.cm.gray)
plt.imshow(Df[:,:,i,:],cmap=my_cmap,interpolation='none')
frame1 = plt.gca()
frame1.axes.get_xaxis().set_visible(False)
frame1.axes.get_yaxis().set_visible(False)
plt.tight_layout(pad=0,w_pad=0,h_pad=0)
In [114]:
C=np.zeros((S[3],3))
i=0
l=0
Betas2=Betas
OdorNuminRegion=np.zeros(12)
for j in range(S[3]):
if GoodICAnat[j] and Betas2[1,j]>0.45*np.max(Betas2[1,:]) and abs(Betas2[0,j])<0.45*np.max(Betas2[0,:]):
#if 1>0.1:
#C[j,:]=C1[i%6][:]
C[j,0]=1
C[j,1]=Betas2[1,j]/np.max(Betas2[1,:])
#C[j,2]=1
for k in range(3):
M=np.max(np.squeeze(np.reshape(D2[:,:,:,j],S[0]*S[1]*5)))
Fmaps[:,:,:,k]=0.25*D2[:,:,:,j]*C[j,k]/M
Final_map=Final_map+Fmaps
#Betas2[1,j]=0
#print(Indexo[j])
OdorNuminRegion[int(CompMainName[j])-1]=OdorNuminRegion[int(CompMainName[j])-1]+1
print(RegionName[int(CompMainName[j])-1])
i=i+1
l=l+1
print(j+1)
#if l==2:
# break
NumOdor=i
print('Number of odor components')
print(i)
In [115]:
pylab.rcParams['figure.figsize'] = (15, 6)
C2=np.zeros(3)
Df=np.zeros([S[0],S[1],5,3])
for i in range(3):
Df[:,:,:,i]=Final_map[:,:,:,i]+Dmean/10
#Df=Df/(np.max(np.max(np.max(Df),3)))
if S[2]>5:
N=Nstack
else:
N=S[2]
for i in range(N):
#if Good_ICs[j]:
plt.subplot(1,N,i+1)
plt.imshow(Df[:,:,i],cmap=plt.cm.gray)
plt.imshow(Df[:,:,i,:],cmap=my_cmap,interpolation='none')
frame1 = plt.gca()
frame1.axes.get_xaxis().set_visible(False)
frame1.axes.get_yaxis().set_visible(False)
plt.tight_layout(pad=0,w_pad=0,h_pad=0)
In [116]:
np.savetxt('/'.join(filename.split('/')[:-1])+'/OdorNumberInLargeRegions.txt',OdorNuminRegion)
np.savetxt('/'.join(filename.split('/')[:-1])+'/LightNumberInLargeRegions.txt',LightNuminRegion)
In [117]:
plt.plot(OdorNuminRegion)
plt.plot(LightNuminRegion)
Out[117]:
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