In [1]:
import matplotlib
import numpy as np
import matplotlib.pyplot as plt
from scipy import stats
from scipy import io
import scipy.io as sio
%matplotlib inline 
import pylab
import csv
from Tkinter import Tk
from tkFileDialog import askopenfilename
from tkFileDialog import askdirectory
import nibabel as nb
from scipy import io
#from nifti import NiftiImage
import nibabel as nb
from scipy.interpolate import interp1d
from scipy import ndimage

Open data


In [2]:
# from http://stackoverflow.com/questions/3579568/choosing-a-file-in-python-with-simple-dialog
Tk().withdraw() # we don't want a full GUI, so keep the root window from appearing
filename = askopenfilename() # show an "Open" dialog box and return the path to the selected file
print(filename)


/media/sophie/db554c18-e3eb-41e2-afad-7de1c92bf4a5/ArclightCombo/918/918ss1regc1000dFF20spsfkf118Smith0_4_60TS.mat

In [3]:
Ua=sio.loadmat(filename)
DT=Ua['TSo']
DT.shape


Out[3]:
(11158, 118)

In [4]:
# from http://stackoverflow.com/questions/3579568/choosing-a-file-in-python-with-simple-dialog
Tk().withdraw() # we don't want a full GUI, so keep the root window from appearing
filename2 = askopenfilename() # show an "Open" dialog box and return the path to the selected file
print(filename2)


/media/sophie/db554c18-e3eb-41e2-afad-7de1c92bf4a5/ArclightCombo/918/918ss1regc1000dFF20spsfkf118Smith0_4_60IC.nii

In [5]:
img1 = nb.load(filename2)
data = img1.get_data()
S=data.shape
S


Out[5]:
(85, 46, 10, 118)

In [6]:
S=data.shape
S


Out[6]:
(85, 46, 10, 118)

Z-score


In [7]:
Demean=np.zeros(S)
Dmaps=np.zeros(S)
Dvar=np.zeros(S)
Var=np.zeros(S[3])
D2=np.zeros([S[0],S[1],5,S[3]])
Tvar=np.zeros(S[3])

In [8]:
for i in range(S[3]):
    Demean[:,:,:,i]=data[:,:,:,i]-np.mean(np.mean(np.mean(data[:,:,:,i],0),0),0)

In [9]:
for i in range(S[3]):
    Dsq=np.reshape(Demean[:,:,:,i],S[0]*S[1]*S[2])
    Var[i]=np.sqrt(np.var(Dsq))
    Dvar=Demean[:,:,:,i]/Var[i]
    Dmaps[:,:,:,i]=Dvar-2.5
    Tvar[i]=np.var(DT[i,:])
Dmaps[Dmaps<0]=0

Open Masks


In [10]:
# from http://stackoverflow.com/questions/3579568/choosing-a-file-in-python-with-simple-dialog
from Tkinter import Tk
from tkFileDialog import askopenfilename

Tk().withdraw() # we don't want a full GUI, so keep the root window from appearing
filenameM = askopenfilename() # show an "Open" dialog box and return the path to the selected file
print(filenameM)
img1 = nb.load(filenameM)
Masks = img1.get_data()
Sm=Masks.shape
Masks=np.array(Masks)


/media/sophie/db554c18-e3eb-41e2-afad-7de1c92bf4a5/ArclightCombo/918/918Registration/JFRCTransformedLargefullpsftrimmed.nii

In [11]:
filenameM='/home/sophie/LargeRegionList'
with open(filenameM) as f:
    content = f.readlines()
Names=[Line.replace('\n','').split(' ') for Line in content]
RegionName=[Names[i][1] for i in range(12)]
Num=[int(Names[i][0]) for i in range(12)]

In [12]:
RegionName


Out[12]:
['OL',
 'VLNP',
 'VMNP',
 'AL',
 'MB',
 'LH',
 'SNP',
 'CX',
 'LX',
 'INP',
 'PENP',
 'GNG']

Average in masks to sort components by brain region


In [13]:
Dmaps.shape


Out[13]:
(85, 46, 10, 118)

In [14]:
M=np.zeros((S[3],13))
Mapmean=np.zeros(S[3])
MMasks=np.zeros(13)

In [15]:
for i in range(S[3]):
    Mapmean[i]=np.mean(np.mean(np.mean(Dmaps[:,:,:,i])))
    for j in range(12):
        MMasks[j]=np.mean(np.mean(np.mean(Masks[:,:,:,j])))
        if MMasks[j]:
            M[i,j]=np.mean(np.mean(np.mean(Masks[:,:,:,j]*Dmaps[:,:,:,i])))/(MMasks[j]*Mapmean[i])

In [16]:
CompMainName=S[3]*['']
CompNameAdd=np.zeros((S[3],12))
for i in range(S[3]):
    Max=np.max(M[i,:])
    I=np.argmax(M[i,:])+1
    for j in range(12):
        J=[l for l in range(12) if Num[l]==(j+1)]
        if M[i,j]>0.2*Max:
            CompNameAdd[i,J]=1
    J=[l for l in range(12) if Num[l]==I]
    if J!= []:
        CompMainName[i]=Names[np.array(J)][0]


/usr/local/lib/python2.7/dist-packages/ipykernel/__main__.py:12: VisibleDeprecationWarning: converting an array with ndim > 0 to an index will result in an error in the future

In [17]:
J


Out[17]:
[7]

In [18]:
pylab.rcParams['figure.figsize'] = (13, 2.5)

h=5
tot=0
GoodICAnat=np.zeros(S[3])

for l in range(12):
    Final_maps=np.zeros((S[0],S[1],3))
    Fmap=np.zeros((S[0],S[1],3))
    C=np.zeros(3)

    n=0
    for i in range(len(CompMainName)):                    
        Dmmv=np.mean(data[:,:,:,i],2) 
        Dmmv[Dmmv<0.2*np.max(np.max(np.max(Dmmv)))]=0
        C=np.squeeze(np.random.rand(3,1))
        labeled, nrobject=ndimage.label(Dmmv>0)
        
        if CompMainName[i]==Names[l][0] and (sum(CompNameAdd[i,:])<5) and nrobject<200:
            n=n+1            
            
            for k in range(3):
                Fmap[:,:,k]=0.7*Dmmv*C[k]/np.max(C)
            Final_maps=Final_maps+Fmap
            #plt.plot(Time_fluoICA.T,(DT[:,i]/np.sqrt(np.var(DT[:,i]))-h*n+2),color=C/2)
            plt.plot((DT[:,i]/np.sqrt(np.var(DT[:,i]))-h*n+2),color=C/2)
            tot=tot+1
            GoodICAnat[i]=1
            
                    
    if n!=0:
        print(RegionName[l])
        plt.show()
        FM=Final_maps/np.max(np.max(Final_maps))
        FM[FM<0.1]=0
        plt.imshow(FM,interpolation='none')
        plt.show()
        frame1 = plt.gca()
        frame1.axes.get_xaxis().set_visible(False)
        frame1.axes.get_yaxis().set_visible(False)


OL
VLNP
VMNP
AL
MB
LH
SNP
CX
INP
PENP
GNG
Looked at the components maps and time series and remove all the components which are localized on the edge of the brain and with activity unlike GCaMP6 transients.

In [49]:
BadICs=[6,73,95,65,25,98,99,116,101,79,23,40,48,77,92,100,76,27,75,43,102,60,67,0,1,2,4,7,8,10,12,16,17,20,30,31,32,38,44,93,13,26,29,39,47,62,33,5,35,41,42,45,46,50,51,56,57,58,59,68,78,81,83,87,96,103,37,84,71,15,21,91,64,80]

In [50]:
for idx in BadICs:
    GoodICAnat[idx] = 0.0

In [51]:
pylab.rcParams['figure.figsize'] = (13, 2.5)

h=5
tot=0
NumberInLargeRegion=np.zeros(13)

for l in range(12):
    Final_maps=np.zeros((S[0],S[1],3))
    Fmap=np.zeros((S[0],S[1],3))
    C=np.zeros(3)

    n=0
    for i in range(len(CompMainName)):                    
        Dmmv=np.mean(data[:,:,:,i],2) 
        Dmmv[Dmmv<0.2*np.max(np.max(np.max(Dmmv)))]=0
        C=np.squeeze(np.random.rand(3,1))
        labeled, nrobject=ndimage.label(Dmmv>0)
        
        if CompMainName[i]==Names[l][0] and (sum(CompNameAdd[i,:])<5) and nrobject<200 and GoodICAnat[i]==1:
            n=n+1            
            
            for k in range(3):
                Fmap[:,:,k]=0.7*Dmmv*(C[k]+0.2)/np.max(C+0.2)
            Final_maps=Final_maps+Fmap
            #plt.plot(Time_fluoICA.T,(DT[:,i]/np.sqrt(np.var(DT[:,i]))-h*n+2),color=C/2)
            plt.plot((DT[:,i]/np.sqrt(np.var(DT[:,i]))-h*n+2),color=C/2)
            tot=tot+1
            GoodICAnat[i]=1
            print(i)
                    
    if n!=0:
        print(RegionName[l])
        plt.show()
        FM=Final_maps/np.max(np.max(Final_maps))
        FM[FM<0.1]=0
        plt.imshow(FM,interpolation='none')
        plt.show()
        frame1 = plt.gca()
        frame1.axes.get_xaxis().set_visible(False)
        frame1.axes.get_yaxis().set_visible(False)
                
    NumberInLargeRegion[l]=n


11
14
28
54
66
74
85
90
OL
9
97
VLNP
18
19
24
53
55
69
70
AL
49
52
72
88
LH
63
SNP
82
105
109
CX
3
22
INP
94
GNG

In [52]:
# Output number of component per region
np.savetxt('/'.join(filename.split('/')[:-1])+'/NumberInLargeRegionsV2.txt',NumberInLargeRegion)

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In [ ]: