'Heavy' window method
Anything found in the 'heavy' fraction is classified as an incorporator.
Methanotrophic bacteria in warm geothermal spring sediments identified using stable-isotope probing
- Christine E. Sharp, Azucena Martínez-Lorenzo, Allyson L. Brady, Stephen E. Grasby, Peter F. Dunfield
- https://femsec.oxfordjournals.org/content/90/1/92
- nucleotide: DNA
- gradient: CsCl
- isotope: 13CH4
- marker: pmoA
- sequencing: pyrosequencing
- heavy window: 1.72-1.74
Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope Probing and Pyrosequencing
- Terrence H. Bell1,2, Etienne Yergeau2, Christine Martineau1,2, David Juck2, Lyle G. Whyte1 and Charles W. Greer2
- http://aem.asm.org/content/77/12/4163.full.pdf+html
- nucleotide: DNA
- gradient: CsCl
- isotope: 15N-MAP
- marker: 16S rRNA
- sequencing: pyrosequencing
- heavy window: 1.727-1.735
Detection of autotrophic verrucomicrobial methanotrophs in a geothermal environment using stable isotope probing
Combining metagenomics with metaproteomics and stable isotope probing reveals metabolic pathways used by a naturally occurring marine methylotroph
- Carolina Grob,1† Martin Taubert, Alexandra M. Howat,1 Oliver J. Burns, Joanna L. Dixon,3 Hans H. Richnow,4, Nico Jehmlich,5 Martin von Bergen,5,6,7 Yin Chen8 and J. Colin Murrell1
- http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12935/full
- nucleotide: DNA
- gradient: CsCl
- isotope: 13C-methanol
- marker: 16S rRNA
- sequencing: pyrosequencing
- heavy window: 1.725
- light window: 1.7
Identification of Cellulose-Responsive Bacterial and Fungal Communities in Geographically and Edaphically Different Soils by Using Stable Isotope Probing
http://onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2011.02501.x/epdf
- nucleotide: DNA
- gradient: CsCl
- isotope: 13C-PAHs
- marker: 16S rRNA
- sequencing: clone library + Sanger
- heavy window: ?!?!?!