In [5]:

    

lines1 = []
with open('1', 'r') as file1:
    for line in file1:
        lines1.append(line.strip())

lines2 = []
with open('2', 'r') as file2:
    for line in file2:
        lines2.append(line.strip())
        
for line in lines2:
    if line not in lines1:
        print line


    

    




AAAGCAAAAAGGGTAT
AAAGTACTTACCACGT
AAATCAAACGAGGCAC
AACTGTCG
AATCACAACGTGAATT
AATTAGGA
ACAACGGG
ACATGCTCTACTCCGT
ACCGACTT
ACCGGTCACGAATCCC
AGAACGGCGGGGTATA
AGATCTGGATAAGCCT
ATACGACATATCACAC
CAAAACTG
CACCACGG
CATCCAGC
CCGGTTAC
CCGTCTTT
CGGTAAGG
CGTCCTGG
CGTGCGAAATTGTTCG
CTAGTAAT
CTGATATTGAGCCGAG
CTGCACCG
CTGTGTCC
GAACACTC
GAATCATGACCGGCCA
GATAGGCT
GCGCAGAC
GGCGCTGAGCTATTCC
GGGCTTGTGCTACAGG
GGTCAGTGGTCCAACA
GTGGGCGC
GTGTACGC
TAGTACAAAATACTAG
TATGTATCGTGCTATT
TATGTGATACTGGGGC
TCCTAGAAGAGTTGATATCGGAGC
TCGTATCCATGATGGG
TGATTGTA
TGCAGAAT
TGTAAGCG
TGTGGGGG
TGTTACAACAGTCCGG
TTGTGCCGATAGGTGA