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In [1]:

    
from pepdata import iedb



In [2]:

    
df = iedb.mhc.load_dataframe(human=True)



In [14]:

    
# keep only Class II epitopes
print("Total entries: %d" % len(df))
df_class2 = df[(df["MHC"]["MHC allele class"] == "II") ]
print("Class II: %d" % len(df_class2))

df_class2 = df_class2[df_class2["Epitope"]["Object Type"] == "Linear peptide"]
print("Class II linear peptides: %d" % len(df_class2))

df_class2 = df_class2[~df_class2["Epitope"]["Description"].str.contains(" ")]
print("Class II linear peptides without modifications: %d" % len(df_class2))









    



Total entries: 533952
Class II: 136236
Class II linear peptides: 136196
Class II linear peptides without modifications: 134555



In [15]:

    
print(df_class2["MHC"].head())
print(df_class2["Epitope"].head())
print(df_class2["Assay"].head())









    



   Allele Name  MHC Allele ID MHC allele class
71     HLA-DR1          327.0               II
72    HLA-DR15          336.0               II
73    HLA-DR11          330.0               II
74    HLA-DR13          333.0               II
75     HLA-DR7          350.0               II
    Epitope ID     Object Type     Description  Starting Position  \
71       57415  Linear peptide  SEFAYGSFVRTVSL               91.0   
72       57415  Linear peptide  SEFAYGSFVRTVSL               91.0   
73       57415  Linear peptide  SEFAYGSFVRTVSL               91.0   
74       57415  Linear peptide  SEFAYGSFVRTVSL               91.0   
75       57415  Linear peptide  SEFAYGSFVRTVSL               91.0   

    Ending Position Non-peptidic epitope ChEBI ID    Antigen Name Antigen ID  \
71            104.0                           NaN  14 kDa antigen   P0A5B7.2   
72            104.0                           NaN  14 kDa antigen   P0A5B7.2   
73            104.0                           NaN  14 kDa antigen   P0A5B7.2   
74            104.0                           NaN  14 kDa antigen   P0A5B7.2   
75            104.0                           NaN  14 kDa antigen   P0A5B7.2   

                 Organism Name  Organism ID Epitope Comments  \
71  Mycobacterium tuberculosis       1773.0              NaN   
72  Mycobacterium tuberculosis       1773.0              NaN   
73  Mycobacterium tuberculosis       1773.0              NaN   
74  Mycobacterium tuberculosis       1773.0              NaN   
75  Mycobacterium tuberculosis       1773.0              NaN   

   Related Object Type  
71                 NaN  
72                 NaN  
73                 NaN  
74                 NaN  
75                 NaN  
                         Method/Technique  \
71  purified MHC/competitive/fluorescence   
72  purified MHC/competitive/fluorescence   
73  purified MHC/competitive/fluorescence   
74  purified MHC/competitive/fluorescence   
75  purified MHC/competitive/fluorescence   

                                     Assay Group Units  Assay Type ID  \
71  half maximal inhibitory concentration (IC50)    nM            273   
72  half maximal inhibitory concentration (IC50)    nM            273   
73  half maximal inhibitory concentration (IC50)    nM            273   
74  half maximal inhibitory concentration (IC50)    nM            273   
75  half maximal inhibitory concentration (IC50)    nM            273   

   Qualitative Measure  Quantitative measurement  Number of Subjects Tested  \
71       Positive-High                    4600.0                        NaN   
72       Positive-High                    7100.0                        NaN   
73       Positive-High                    5100.0                        NaN   
74       Positive-High                    2000.0                        NaN   
75       Positive-High                    6100.0                        NaN   

    Number of Subjects Responded  Response Frequency 3D complex ID  
71                           NaN                 NaN           NaN  
72                           NaN                 NaN           NaN  
73                           NaN                 NaN           NaN  
74                           NaN                 NaN           NaN  
75                           NaN                 NaN           NaN



In [ ]:



In [42]:

    
from collections import defaultdict, OrderedDict
columns = OrderedDict()
columns["mhc"] = df_class2["MHC"]["Allele Name"]
columns["peptide"] = df_class2["Epitope"]["Description"]
columns["units"] = df_class2["Assay"]["Units"]
columns["meas"] = df_class2["Assay"]["Quantitative measurement"]
columns["assay_group"] = df_class2["Assay"]["Assay Group"]
columns["assay_method"] = df_class2["Assay"]["Method/Technique"]
columns["qual"] = df_class2["Assay"]["Qualitative Measure"]

df_simple = pd.DataFrame(columns)
df_simple.to_csv("class2_data.csv", index=False)



In [17]:

    
df_simple









    Out[17]:






  
    
      
      mhc
      peptide
      units
      meas
      assay_group
      assay_method
      qual
    
  
  
    
      71
      HLA-DR1
      SEFAYGSFVRTVSL
      nM
      4600.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      72
      HLA-DR15
      SEFAYGSFVRTVSL
      nM
      7100.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      73
      HLA-DR11
      SEFAYGSFVRTVSL
      nM
      5100.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      74
      HLA-DR13
      SEFAYGSFVRTVSL
      nM
      2000.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      75
      HLA-DR7
      SEFAYGSFVRTVSL
      nM
      6100.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      78
      HLA-DRB1*03:01
      LTNQNINIDQEFNLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      80
      HLA-DRB1*04:01
      YTNQNINISMERNLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      102
      HLA-DRB1*03:01
      LTNQNINIDQEFPLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      103
      HLA-DRB1*01:01
      LTNQNINIDQEFNLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Negative
    
    
      104
      HLA-DRB1*04:01
      LTNQNINIDQEFNLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      105
      HLA-DRB1*11:01
      LTNQNINIDQEFNLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      106
      HLA-DRB1*01:01
      YTNQNINISMERNLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Negative
    
    
      107
      HLA-DRB1*03:01
      YTNQNINISMERNLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Negative
    
    
      108
      HLA-DRB1*11:01
      YTNQNINISMERNLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Negative
    
    
      109
      HLA-DRB1*01:01
      LTNQNINIDQEFPLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Negative
    
    
      110
      HLA-DRB1*04:01
      LTNQNINIDQEFPLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      111
      HLA-DRB1*11:01
      LTNQNINIDQEFPLMKHGFH
      NaN
      NaN
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      160
      HLA-DRB1*15:01
      EKAKYEAYKAAAAAA
      NaN
      18.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      161
      HLA-DRB1*15:01
      EKPKYEAYKAAAAPA
      NaN
      14.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      162
      HLA-DRB1*15:01
      EKPKFEAYKAAAAPA
      NaN
      26.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      163
      HLA-DRB1*15:01
      EKAKEEAYKAAAAAA
      NaN
      0.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Negative
    
    
      164
      HLA-DRB1*15:01
      EKPKEEAFKAAAAPA
      NaN
      0.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Negative
    
    
      165
      HLA-DRB1*15:01
      EKAKFEAFKAAAAAA
      NaN
      19.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      166
      HLA-DRB1*15:01
      EKPKVEAYKAAAAPA
      NaN
      41.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      167
      HLA-DRB1*15:01
      APEKAKFEAFKAAAAPA
      NaN
      34.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      168
      HLA-DRB1*15:01
      APEKAKFEAYKAAAAPA
      NaN
      31.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      169
      HLA-DRB1*15:01
      APEKAKVEAFKAAAAPA
      NaN
      5.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      170
      HLA-DRB1*15:01
      EAKKYEAYKAAAAAA
      NaN
      0.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Negative
    
    
      171
      HLA-DRB1*15:01
      EAPKFEAYKAAAAPA
      NaN
      13.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      172
      HLA-DRB1*15:01
      EAPKVEAYKAAAAPA
      NaN
      44.0
      qualitative binding
      purified MHC/competitive/fluorescence
      Positive
    
    
      ...
      ...
      ...
      ...
      ...
      ...
      ...
      ...
    
    
      581609
      HLA-DQA1*01:02/DQB1*06:02
      ENEVEPVDARPAADR
      nM
      327.2
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      581610
      HLA-DQA1*01:02/DQB1*06:02
      GVVRGASIPFQFRP
      nM
      174.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      581611
      HLA-DQA1*01:02/DQB1*06:02
      IINEPTAAAIAYG
      nM
      94.7
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      581612
      HLA-DQA1*01:02/DQB1*06:02
      SSQPAVLQPSVE
      nM
      100.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      581613
      HLA-DQA1*01:02/DQB1*06:02
      APSIGFVSVRQGALS
      nM
      158.7
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      581614
      HLA-DQA1*01:02/DQB1*06:02
      EVQQTAARIAGALG
      nM
      105.9
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      581615
      HLA-DQA1*01:02/DQB1*06:02
      TPKTVKGVIIQGARGGD
      nM
      50.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      581616
      HLA-DQA1*01:02/DQB1*06:02
      LPKPPKPVSKMRMATPLLMQA
      nM
      7649.7
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      581617
      HLA-DQA1*01:02/DQB1*06:02
      MATPLLMQALPM
      nM
      3000.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581618
      HLA-DQA1*01:02/DQB1*06:02
      GADSVPANTENEVEPV
      nM
      8026.7
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      581619
      HLA-DQA1*01:02/DQB1*06:02
      VGEQDGGLIGAEEKVIN
      nM
      9000.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      581620
      HLA-DQA1*01:02/DQB1*06:02
      LARCPEAGLAAQVISPLLTPKA
      nM
      3000.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581621
      HLA-DQA1*01:02/DQB1*06:02
      DHGSTGILVFPNEDL
      nM
      321.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      581622
      HLA-DQA1*01:02/DQB1*06:02
      SPLGPLAGSPVIAAANPL
      nM
      122.8
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      581623
      HLA-DQA1*01:02/DQB1*06:02
      KMRMATPLLMQA
      nM
      18000.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      581624
      HLA-DQA1*05:01/DQB1*02:01
      LPKPPKPVSKMRMATPLLMQA
      nM
      NaN
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Negative
    
    
      581625
      HLA-DQA1*03:01/DQB1*03:02
      LPKPPKPVSKMRMATPLLMQA
      nM
      NaN
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Negative
    
    
      581626
      HLA-DQA1*03:01/DQB1*03:02
      GADSVPANTENEVEPV
      nM
      NaN
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Negative
    
    
      581627
      HLA-DQA1*01:02/DQB1*06:02
      LPKPPKPVSKMRMATPLLMQA
      nM
      426.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      581628
      HLA-DQA1*05:01/DQB1*02:01
      LPKPPKPVSKMRMATPLLMQA
      nM
      462.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      581629
      HLA-DQA1*03:01/DQB1*03:02
      LPKPPKPVSKMRMATPLLMQA
      nM
      3498.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581630
      HLA-DQA1*01:02/DQB1*06:02
      ENEVEPVDARPAADR
      nM
      3300.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581631
      HLA-DQA1*01:02/DQB1*06:02
      GVVRGASIPFQFRP
      nM
      3852.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581632
      HLA-DQA1*01:02/DQB1*06:02
      IINEPTAAAIAYG
      nM
      3852.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581633
      HLA-DQA1*01:02/DQB1*06:02
      APSIGFVSVRQGALS
      nM
      4812.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581634
      HLA-DQA1*01:02/DQB1*06:02
      EVQQTAARIAGALG
      nM
      3726.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581635
      HLA-DQA1*01:02/DQB1*06:02
      GADSVPANTENEVEPV
      nM
      8250.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      581636
      HLA-DQA1*01:02/DQB1*06:02
      DHGSTGILVFPNEDL
      nM
      3852.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581637
      HLA-DQA1*01:02/DQB1*06:02
      SPLGPLAGSPVIAAANPL
      nM
      1752.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      581638
      HLA-DQA1*03:01/DQB1*03:02
      GADSVPANTENEVEPV
      nM
      7218.0
      dissociation constant KD (~IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
  

134555 rows × 7 columns



In [18]:

    
df_simple.peptide.str.len().value_counts()









    Out[18]:





15    79664
20     9028
16     7803
14     7095
13     6678
17     6189
12     3439
18     3357
11     2130
19     2002
21     1585
9      1356
10     1347
22      562
23      433
24      373
8       361
25      273
26      147
27      137
30      108
31      107
28       81
29       64
32       61
33       36
34       36
35       30
7        25
5        13
6        10
36        7
37        4
4         4
2         3
43        2
38        2
40        1
3         1
46        1
Name: peptide, dtype: int64



In [20]:

    
df_simple[df_simple.peptide.str.len() > 40]









    Out[20]:






  
    
      
      mhc
      peptide
      units
      meas
      assay_group
      assay_method
      qual
    
  
  
    
      180285
      HLA-DQA1*05:01/DQB1*02:01
      SSIPEELPHNPLEHDPLHPRHFDGGEHNAQFDHEAFLGPDESK
      NaN
      NaN
      ligand presentation
      secreted MHC/mass spectrometry
      Positive
    
    
      343151
      HLA-DRA*01:01/DRB1*08:01
      IVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQL
      NaN
      NaN
      ligand presentation
      cellular MHC/mass spectrometry
      Positive
    
    
      493379
      HLA-DRB1*01:01
      SAAKTKVEKKKKKEKVLATVTKTVGGDKNGGTRVVKLRKMPRYYPT
      NaN
      NaN
      ligand presentation
      cellular MHC/mass spectrometry
      Positive



In [35]:

    
df_simple[df_simple.peptide.str.contains("FLSELTQQLAQ")]









    Out[35]:






  
    
      
      mhc
      peptide
      units
      meas
      assay_group
      assay_method
      qual
    
  
  
    
      343151
      HLA-DRA*01:01/DRB1*08:01
      IVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQL
      NaN
      NaN
      ligand presentation
      cellular MHC/mass spectrometry
      Positive
    
    
      432558
      HLA-DQA1*02:01/DQB1*02:02
      VPDGFLSELTQQLAQ
      NaN
      NaN
      ligand presentation
      cellular MHC/mass spectrometry
      Positive
    
    
      432559
      HLA-DQA1*02:01/DQB1*02:02
      VPDGFLSELTQQLAQA
      NaN
      NaN
      ligand presentation
      cellular MHC/mass spectrometry
      Positive
    
    
      471493
      HLA-DQA1*05:01/DQB1*02:01
      VPDGFLSELTQQLAQ
      NaN
      NaN
      ligand presentation
      cellular MHC/mass spectrometry
      Positive



In [41]:

    
df_simple[df_simple.assay_group == "half maximal inhibitory concentration (IC50)"]









    Out[41]:






  
    
      
      mhc
      peptide
      units
      meas
      assay_group
      assay_method
      qual
    
  
  
    
      71
      HLA-DR1
      SEFAYGSFVRTVSL
      nM
      4600.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      72
      HLA-DR15
      SEFAYGSFVRTVSL
      nM
      7100.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      73
      HLA-DR11
      SEFAYGSFVRTVSL
      nM
      5100.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      74
      HLA-DR13
      SEFAYGSFVRTVSL
      nM
      2000.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      75
      HLA-DR7
      SEFAYGSFVRTVSL
      nM
      6100.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      178
      HLA-DRB1*15:01
      MEVGWYRSPFSRVVHLYRNGK
      nM
      1600.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      179
      HLA-DRB1*15:01
      MEVGWYRPPFSRVVHLYRNGK
      nM
      13500.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      265
      HLA-DRB1*01:01
      PHHTALRQAILCWGELMTLA
      nM
      70.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      267
      HLA-DRB1*04:01
      PHHTALRQAILCWGELMTLA
      nM
      85.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      269
      HLA-DRB1*07:01
      PHHTALRQAILCWGELMTLA
      nM
      676.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      270
      HLA-DRB1*08:02
      PHHTALRQAILCWGELMTLA
      nM
      196.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      271
      HLA-DRB1*09:01
      PHHTALRQAILCWGELMTLA
      nM
      2273.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Low
    
    
      273
      HLA-DRB1*12:01
      PHHTALRQAILCWGELMTLA
      nM
      263.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      275
      HLA-DRB1*15:01
      PHHTALRQAILCWGELMTLA
      nM
      9.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      278
      HLA-DRB5*01:01
      PHHTALRQAILCWGELMTLA
      nM
      211.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      398
      HLA-DRB1*01:01
      AGFFLLTRILTIPQS
      nM
      1.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      400
      HLA-DRB1*04:01
      AGFFLLTRILTIPQS
      nM
      8.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      401
      HLA-DRB1*04:05
      AGFFLLTRILTIPQS
      nM
      253.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      402
      HLA-DRB1*07:01
      AGFFLLTRILTIPQS
      nM
      8.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      403
      HLA-DRB1*08:02
      AGFFLLTRILTIPQS
      nM
      188.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      404
      HLA-DRB1*09:01
      AGFFLLTRILTIPQS
      nM
      58.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      406
      HLA-DRB1*12:01
      AGFFLLTRILTIPQS
      nM
      6.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      407
      HLA-DRB1*13:02
      AGFFLLTRILTIPQS
      nM
      10.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      408
      HLA-DRB1*15:01
      AGFFLLTRILTIPQS
      nM
      217.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      411
      HLA-DRB5*01:01
      AGFFLLTRILTIPQS
      nM
      1053.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Low
    
    
      412
      HLA-DRB1*01:01
      LVPFVQWFVGLSPTV
      nM
      385.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      414
      HLA-DRB1*04:01
      LVPFVQWFVGLSPTV
      nM
      300.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      415
      HLA-DRB1*04:05
      LVPFVQWFVGLSPTV
      nM
      27.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      416
      HLA-DRB1*07:01
      LVPFVQWFVGLSPTV
      nM
      2717.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Low
    
    
      417
      HLA-DRB1*08:02
      LVPFVQWFVGLSPTV
      nM
      74.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      ...
      ...
      ...
      ...
      ...
      ...
      ...
      ...
    
    
      514714
      HLA-DRB1*11:01
      DTFRKDFRVYDNFLR
      nM
      25.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      514715
      HLA-DRB1*13:02
      DTFRKDFRVYDNFLR
      nM
      467.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      514716
      HLA-DRB1*15:01
      DTFRKDFRVYDNFLR
      nM
      96.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      514718
      HLA-DRB5*01:01
      DTFRKDFRVYDNFLR
      nM
      677.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      514719
      HLA-DRB1*01:01
      DTFRKDFRVYDNFLR
      nM
      5.4
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-High
    
    
      514721
      HLA-DQB1*03:01
      DTFRKDFRVYDNFLR
      nM
      8491.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Low
    
    
      514722
      HLA-DRB1*07:01
      DTFRKDFRVYDNFLR
      nM
      630.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      514724
      HLA-DRB1*08:02
      DTFRKDFRVYDNFLR
      nM
      142.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/radioactivity
      Positive-Intermediate
    
    
      521697
      HLA-DRB1*01:01
      SINKLVYLGKLFVTLNQ
      nM
      4600.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      521698
      HLA-DRB1*01:01
      LKETILDLAALISNMNL
      nM
      2387.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      521699
      HLA-DRB1*01:01
      MGSKIQNLFSAFPIHAF
      nM
      324.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      521700
      HLA-DRB1*01:01
      TRTHYLLLAKNGSVFEM
      nM
      74.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      521701
      HLA-DRB1*01:01
      LQLTFFFPLGIYIPTET
      nM
      36521.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Negative
    
    
      521702
      HLA-DRB1*01:01
      MQTLLRKSPPQFIVLTM
      nM
      7876.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Negative
    
    
      521703
      HLA-DRB1*01:01
      NLVSLIRLVKRTISISN
      nM
      2276.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      521704
      HLA-DRB1*01:01
      DVDELKTLYNTFILWLM
      nM
      810.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      521705
      HLA-DRB1*01:01
      PIGSIQKLTNILSQYIS
      nM
      137.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Intermediate
    
    
      521706
      HLA-DRB1*01:01
      KLFIYIITQAWTASEDS
      nM
      37.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      521707
      HLA-DRB1*01:01
      IVLNIFALPYANVTVSN
      nM
      5006.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Negative
    
    
      521708
      HLA-DRB1*01:01
      HYSNYAIGETIPLQLII
      nM
      11.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-High
    
    
      521709
      HLA-DRB1*01:01
      LFLAVFLMNSVLMIYSD
      nM
      4210.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      521710
      HLA-DRB1*01:01
      ELRYFKSGLGISPPSSS
      nM
      3858.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      532188
      HLA-DRA*01:01/DRB1*01:01
      TSKGLFRAAVPSGAS
      nM
      1400.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive
    
    
      532194
      HLA-DRA*01:01/DRB1*01:01
      MSILKIHAREIFDSR
      nM
      3500.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive
    
    
      532200
      HLA-DRA*01:01/DRB1*01:01
      IFDSRGNPTVEVDLF
      nM
      NaN
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Negative
    
    
      532205
      HLA-DRA*01:01/DRB1*01:01
      RYMGKGVSKAVEHIN
      nM
      NaN
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Negative
    
    
      532212
      HLA-DRA*01:01/DRB1*01:01
      LPVGAANFREAMRIG
      nM
      NaN
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Negative
    
    
      532218
      HLA-DRA*01:01/DRB1*01:01
      VIGMDVAASEFFRSG
      nM
      35000.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      532224
      HLA-DRA*01:01/DRB1*01:01
      KRIAKAVNEKSCNCL
      nM
      50000.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
    
      532229
      HLA-DRA*01:01/DRB1*01:01
      KAKFAGRNFRNPLAK
      nM
      50000.0
      half maximal inhibitory concentration (IC50)
      purified MHC/competitive/fluorescence
      Positive-Low
    
  

40424 rows × 7 columns



In [ ]:

	mhc	peptide	units	meas	assay_group	assay_method	qual
71	HLA-DR1	SEFAYGSFVRTVSL	nM	4600.0	half maximal inhibitory concentration (IC50)	purified MHC/competitive/fluorescence	Positive-High
72	HLA-DR15	SEFAYGSFVRTVSL	nM	7100.0	half maximal inhibitory concentration (IC50)	purified MHC/competitive/fluorescence	Positive-High
73	HLA-DR11	SEFAYGSFVRTVSL	nM	5100.0	half maximal inhibitory concentration (IC50)	purified MHC/competitive/fluorescence	Positive-High
74	HLA-DR13	SEFAYGSFVRTVSL	nM	2000.0	half maximal inhibitory concentration (IC50)	purified MHC/competitive/fluorescence	Positive-High
75	HLA-DR7	SEFAYGSFVRTVSL	nM	6100.0	half maximal inhibitory concentration (IC50)	purified MHC/competitive/fluorescence	Positive-High
78	HLA-DRB1*03:01	LTNQNINIDQEFNLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Positive
80	HLA-DRB1*04:01	YTNQNINISMERNLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Positive
102	HLA-DRB1*03:01	LTNQNINIDQEFPLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Positive
103	HLA-DRB1*01:01	LTNQNINIDQEFNLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Negative
104	HLA-DRB1*04:01	LTNQNINIDQEFNLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Positive
105	HLA-DRB1*11:01	LTNQNINIDQEFNLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Positive
106	HLA-DRB1*01:01	YTNQNINISMERNLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Negative
107	HLA-DRB1*03:01	YTNQNINISMERNLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Negative
108	HLA-DRB1*11:01	YTNQNINISMERNLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Negative
109	HLA-DRB1*01:01	LTNQNINIDQEFPLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Negative
110	HLA-DRB1*04:01	LTNQNINIDQEFPLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Positive
111	HLA-DRB1*11:01	LTNQNINIDQEFPLMKHGFH	NaN	NaN	qualitative binding	purified MHC/competitive/fluorescence	Positive
160	HLA-DRB1*15:01	EKAKYEAYKAAAAAA	NaN	18.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
161	HLA-DRB1*15:01	EKPKYEAYKAAAAPA	NaN	14.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
162	HLA-DRB1*15:01	EKPKFEAYKAAAAPA	NaN	26.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
163	HLA-DRB1*15:01	EKAKEEAYKAAAAAA	NaN	0.0	qualitative binding	purified MHC/competitive/fluorescence	Negative
164	HLA-DRB1*15:01	EKPKEEAFKAAAAPA	NaN	0.0	qualitative binding	purified MHC/competitive/fluorescence	Negative
165	HLA-DRB1*15:01	EKAKFEAFKAAAAAA	NaN	19.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
166	HLA-DRB1*15:01	EKPKVEAYKAAAAPA	NaN	41.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
167	HLA-DRB1*15:01	APEKAKFEAFKAAAAPA	NaN	34.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
168	HLA-DRB1*15:01	APEKAKFEAYKAAAAPA	NaN	31.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
169	HLA-DRB1*15:01	APEKAKVEAFKAAAAPA	NaN	5.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
170	HLA-DRB1*15:01	EAKKYEAYKAAAAAA	NaN	0.0	qualitative binding	purified MHC/competitive/fluorescence	Negative
171	HLA-DRB1*15:01	EAPKFEAYKAAAAPA	NaN	13.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
172	HLA-DRB1*15:01	EAPKVEAYKAAAAPA	NaN	44.0	qualitative binding	purified MHC/competitive/fluorescence	Positive
...	...	...	...	...	...	...	...
581609	HLA-DQA101:02/DQB106:02	ENEVEPVDARPAADR	nM	327.2	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-High
581610	HLA-DQA101:02/DQB106:02	GVVRGASIPFQFRP	nM	174.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-High
581611	HLA-DQA101:02/DQB106:02	IINEPTAAAIAYG	nM	94.7	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-High
581612	HLA-DQA101:02/DQB106:02	SSQPAVLQPSVE	nM	100.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-High
581613	HLA-DQA101:02/DQB106:02	APSIGFVSVRQGALS	nM	158.7	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-High
581614	HLA-DQA101:02/DQB106:02	EVQQTAARIAGALG	nM	105.9	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-High
581615	HLA-DQA101:02/DQB106:02	TPKTVKGVIIQGARGGD	nM	50.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-High
581616	HLA-DQA101:02/DQB106:02	LPKPPKPVSKMRMATPLLMQA	nM	7649.7	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Low
581617	HLA-DQA101:02/DQB106:02	MATPLLMQALPM	nM	3000.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581618	HLA-DQA101:02/DQB106:02	GADSVPANTENEVEPV	nM	8026.7	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Low
581619	HLA-DQA101:02/DQB106:02	VGEQDGGLIGAEEKVIN	nM	9000.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Low
581620	HLA-DQA101:02/DQB106:02	LARCPEAGLAAQVISPLLTPKA	nM	3000.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581621	HLA-DQA101:02/DQB106:02	DHGSTGILVFPNEDL	nM	321.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-High
581622	HLA-DQA101:02/DQB106:02	SPLGPLAGSPVIAAANPL	nM	122.8	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-High
581623	HLA-DQA101:02/DQB106:02	KMRMATPLLMQA	nM	18000.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Low
581624	HLA-DQA105:01/DQB102:01	LPKPPKPVSKMRMATPLLMQA	nM	NaN	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Negative
581625	HLA-DQA103:01/DQB103:02	LPKPPKPVSKMRMATPLLMQA	nM	NaN	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Negative
581626	HLA-DQA103:01/DQB103:02	GADSVPANTENEVEPV	nM	NaN	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Negative
581627	HLA-DQA101:02/DQB106:02	LPKPPKPVSKMRMATPLLMQA	nM	426.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Low
581628	HLA-DQA105:01/DQB102:01	LPKPPKPVSKMRMATPLLMQA	nM	462.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Low
581629	HLA-DQA103:01/DQB103:02	LPKPPKPVSKMRMATPLLMQA	nM	3498.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581630	HLA-DQA101:02/DQB106:02	ENEVEPVDARPAADR	nM	3300.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581631	HLA-DQA101:02/DQB106:02	GVVRGASIPFQFRP	nM	3852.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581632	HLA-DQA101:02/DQB106:02	IINEPTAAAIAYG	nM	3852.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581633	HLA-DQA101:02/DQB106:02	APSIGFVSVRQGALS	nM	4812.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581634	HLA-DQA101:02/DQB106:02	EVQQTAARIAGALG	nM	3726.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581635	HLA-DQA101:02/DQB106:02	GADSVPANTENEVEPV	nM	8250.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Low
581636	HLA-DQA101:02/DQB106:02	DHGSTGILVFPNEDL	nM	3852.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581637	HLA-DQA101:02/DQB106:02	SPLGPLAGSPVIAAANPL	nM	1752.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Intermediate
581638	HLA-DQA103:01/DQB103:02	GADSVPANTENEVEPV	nM	7218.0	dissociation constant KD (~IC50)	purified MHC/competitive/fluorescence	Positive-Low

	mhc	peptide	units	meas	assay_group	assay_method	qual
180285	HLA-DQA105:01/DQB102:01	SSIPEELPHNPLEHDPLHPRHFDGGEHNAQFDHEAFLGPDESK	NaN	NaN	ligand presentation	secreted MHC/mass spectrometry	Positive
343151	HLA-DRA01:01/DRB108:01	IVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQL	NaN	NaN	ligand presentation	cellular MHC/mass spectrometry	Positive
493379	HLA-DRB1*01:01	SAAKTKVEKKKKKEKVLATVTKTVGGDKNGGTRVVKLRKMPRYYPT	NaN	NaN	ligand presentation	cellular MHC/mass spectrometry	Positive