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from bokeh.io import output_notebook
output_notebook()


    

    






    

        
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from cameo import models
from marsi.cobra.strain_design import OptMet


    

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model = models.bigg.iJO1366


    

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optimization = OptMet(model=model)


    

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target = model.metabolites.succ_c
biomass = model.reactions.BIOMASS_Ec_iJO1366_core_53p95M
substrate = model.reactions.EX_glc__D_e

# Succinate production needs to be anaerobic to yield growth coupled designs.
model.reactions.EX_o2_e.lower_bound = 0


    

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result = optimization.run(target=target, biomass=biomass, substrate=substrate, max_evaluations=3000)


    

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result


    

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