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from pydna.all import *
In [2]:
pYPKa=read("pYPKa.gb")
from Bio.Restriction import ZraI, AjiI, EcoRV
gb =Genbank("bjornjobbb@gmail.com")
enz = AjiI
gene = gb.nucleotide("AJ937350") # Candida intermedia gxf1 gene for glucose/xylose facilitator 1
primers =parse('''
>553_GXF1_YPK_fwd (29-mer)
CGCGCCAAAatgtcacaagattcgcattc
>552_GXF1_YPK_rev (22-mer)
GGttaaacctgttcgtcggtgg
''', ds=False)
pYPKa_cut = pYPKa.cut(enz)[0]
gene =pcr( primers, gene)
pYPKa_enz_gene = (pYPKa_cut + gene).looped()
pYPKa_enz_gene = pYPKa_enz_gene.synced(pYPKa)
pYPKa_A_CiGXF1 = pYPKa_enz_gene
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pYPKa_A_CiGXF1.write("pYPKa_A_CiGXF1.gb")
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