In [1]:
from pydna.all import *

In [2]:
pYPKa=read("pYPKa.gb")


from Bio.Restriction import ZraI, AjiI, EcoRV

gb =Genbank("bjornjobbb@gmail.com")

enz = AjiI

gene = gb.nucleotide("AJ937350") # Candida intermedia gxf1 gene for glucose/xylose facilitator 1

primers =parse('''
>553_GXF1_YPK_fwd (29-mer)
CGCGCCAAAatgtcacaagattcgcattc

>552_GXF1_YPK_rev (22-mer)
GGttaaacctgttcgtcggtgg
''', ds=False)

pYPKa_cut = pYPKa.cut(enz)[0]

gene =pcr( primers, gene)

pYPKa_enz_gene = (pYPKa_cut + gene).looped()

pYPKa_enz_gene = pYPKa_enz_gene.synced(pYPKa)

pYPKa_A_CiGXF1 = pYPKa_enz_gene

In [3]:
pYPKa_A_CiGXF1.write("pYPKa_A_CiGXF1.gb")





In [4]: