In [1]:
from pydna.all import *
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p426TEF1 =read("./Mumberg/p426TEF.gb")
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p426TEF1
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pYPKa_A_HIS3 =read("pYPKa_A_ScHIS3.gb")
In [5]:
f, r =parse('''
>HIS3_mum_fwd
TCTAGAACTAGTGGATCCCCCGGGCTGCAGGAAAATGACAGAGCAGAAAGCC
>HIS3_mum_rev
CTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCTACATAAGAACACCTTTGGTGGAG''', ds=False)
In [6]:
HIS3_prod =pcr(f,r, pYPKa_A_HIS3)
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from Bio.Restriction import EcoRI
p426TEF1_lin = p426TEF1.linearize(EcoRI)
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asm =Assembly((p426TEF1_lin, HIS3_prod))
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asm
Out[9]:
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candidate = asm.assemble_circular()[0]
candidate.figure()
Out[10]:
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final = candidate.synced(p426TEF1)
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final.write("p426TEF1_HIS3.gb")
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