p426TEF1_HIS3


In [1]:
from pydna.all import *

In [2]:
p426TEF1 =read("./Mumberg/p426TEF.gb")

In [3]:
p426TEF1





In [4]:
pYPKa_A_HIS3 =read("pYPKa_A_ScHIS3.gb")

In [5]:
f, r =parse('''
>HIS3_mum_fwd 
TCTAGAACTAGTGGATCCCCCGGGCTGCAGGAAAATGACAGAGCAGAAAGCC
>HIS3_mum_rev 
CTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCTACATAAGAACACCTTTGGTGGAG''', ds=False)

In [6]:
HIS3_prod =pcr(f,r, pYPKa_A_HIS3)

In [7]:
from Bio.Restriction import EcoRI
p426TEF1_lin = p426TEF1.linearize(EcoRI)

In [8]:
asm =Assembly((p426TEF1_lin, HIS3_prod))

In [9]:
asm


Out[9]:
Assembly
fragments..: 6356bp 735bp
limit(bp)..: 25
G.nodes....: 4
algorithm..: common_sub_strings

In [10]:
candidate = asm.assemble_circular()[0]
candidate.figure()


Out[10]:
 -|name_lin|33
|           \/
|           /\
|           33|735bp_PCR_prod|38
|                             \/
|                             /\
|                             38-
|                                |
 --------------------------------

In [11]:
final = candidate.synced(p426TEF1)

In [12]:
final.write("p426TEF1_HIS3.gb")





In [13]: