p426TDH3_HIS3

In [1]:

    

from pydna.all import *


    

In [2]:

    

p426TDH3 =read("./Mumberg/p426GPD.gb")


    

In [3]:

    

p426TDH3


    

    
Out[3]:




./Mumberg/p426GPD.gb

In [4]:

    

pYPKa_A_HIS3 =read("pYPKa_A_ScHIS3.gb")


    

In [5]:

    

f, r =parse('''
>HIS3_mum_fwd 
TCTAGAACTAGTGGATCCCCCGGGCTGCAGGAAAATGACAGAGCAGAAAGCC
>HIS3_mum_rev 
CTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCTACATAAGAACACCTTTGGTGGAG''', ds=False)


    

In [6]:

    

HIS3_prod =pcr(f,r, pYPKa_A_HIS3)


    

In [7]:

    

from Bio.Restriction import EcoRI
p426TDH3_lin = p426TDH3.linearize(EcoRI)


    

In [8]:

    

asm =Assembly((p426TDH3_lin, HIS3_prod))


    

In [9]:

    

asm


    

    
Out[9]:





Assembly
fragments..: 6610bp 735bp
limit(bp)..: 25
G.nodes....: 4
algorithm..: common_sub_strings

In [10]:

    

candidate = asm.assemble_circular()[0]
candidate.figure()


    

    
Out[10]:





 -|name_lin|33
|           \/
|           /\
|           33|735bp_PCR_prod|38
|                             \/
|                             /\
|                             38-
|                                |
 --------------------------------

In [11]:

    

final = candidate.synced(p426TDH3)


    

In [12]:

    

final.write("p426TDH3_HIS3.gb")


    

    





p426TDH3_HIS3.gb