```
In [ ]:
```from __future__ import print_function, division
import numpy
import scipy.stats
import matplotlib.pyplot as pyplot
import first
# some nicer colors from http://colorbrewer2.org/
COLOR1 = '#7fc97f'
COLOR2 = '#beaed4'
COLOR3 = '#fdc086'
COLOR4 = '#ffff99'
COLOR5 = '#386cb0'
%matplotlib inline

Suppose you observe an apparent difference between two groups and you want to check whether it might be due to chance.

As an example, we'll look at differences between first babies and others. The `first`

module provides code to read data from the National Survey of Family Growth (NSFG).

```
In [ ]:
```live, firsts, others = first.MakeFrames()

We'll look at a couple of variables, including pregnancy length and birth weight. The effect size we'll consider is the difference in the means.

Other examples might include a correlation between variables or a coefficient in a linear regression. The number that quantifies the size of the effect is called the "test statistic".

```
In [ ]:
```def TestStatistic(data):
group1, group2 = data
test_stat = abs(group1.mean() - group2.mean())
return test_stat

```
In [ ]:
```group1 = firsts.prglngth
group2 = others.prglngth

The actual difference in the means is 0.078 weeks, which is only 13 hours.

```
In [ ]:
```actual = TestStatistic((group1, group2))
actual

```
In [ ]:
```n, m = len(group1), len(group2)
pool = numpy.hstack((group1, group2))

```
In [ ]:
```def RunModel():
numpy.random.shuffle(pool)
data = pool[:n], pool[n:]
return data

The result of running the model is two NumPy arrays with the shuffled pregnancy lengths:

```
In [ ]:
```RunModel()

Then we compute the same test statistic using the simulated data:

```
In [ ]:
```TestStatistic(RunModel())

```
In [ ]:
```test_stats = numpy.array([TestStatistic(RunModel()) for i in range(1000)])
test_stats.shape

```
In [ ]:
```pyplot.axvline(actual, linewidth=3, color='0.8')
pyplot.hist(test_stats, color=COLOR5)
pyplot.xlabel('difference in means')
pyplot.ylabel('count')
None

```
In [ ]:
```pvalue = sum(test_stats >= actual) / len(test_stats)
pvalue

In this case the result is about 15%, which means that even if there is no difference between the groups, it is plausible that we could see a sample difference as big as 0.078 weeks.

We conclude that the apparent effect might be due to chance, so we are not confident that it would appear in the general population, or in another sample from the same population.

```
In [ ]:
```class HypothesisTest(object):
"""Represents a hypothesis test."""
def __init__(self, data):
"""Initializes.
data: data in whatever form is relevant
"""
self.data = data
self.MakeModel()
self.actual = self.TestStatistic(data)
self.test_stats = None
def PValue(self, iters=1000):
"""Computes the distribution of the test statistic and p-value.
iters: number of iterations
returns: float p-value
"""
self.test_stats = numpy.array([self.TestStatistic(self.RunModel())
for _ in range(iters)])
count = sum(self.test_stats >= self.actual)
return count / iters
def MaxTestStat(self):
"""Returns the largest test statistic seen during simulations.
"""
return max(self.test_stats)
def PlotHist(self, label=None):
"""Draws a Cdf with vertical lines at the observed test stat.
"""
pyplot.hist(ht.test_stats, color=COLOR4)
pyplot.axvline(self.actual, linewidth=3, color='0.8')
pyplot.xlabel('test statistic')
pyplot.ylabel('count')
def TestStatistic(self, data):
"""Computes the test statistic.
data: data in whatever form is relevant
"""
raise UnimplementedMethodException()
def MakeModel(self):
"""Build a model of the null hypothesis.
"""
pass
def RunModel(self):
"""Run the model of the null hypothesis.
returns: simulated data
"""
raise UnimplementedMethodException()

`HypothesisTest`

is an abstract parent class that encodes the template. Child classes fill in the missing methods. For example, here's the test from the previous section.

```
In [ ]:
```class DiffMeansPermute(HypothesisTest):
"""Tests a difference in means by permutation."""
def TestStatistic(self, data):
"""Computes the test statistic.
data: data in whatever form is relevant
"""
group1, group2 = data
test_stat = abs(group1.mean() - group2.mean())
return test_stat
def MakeModel(self):
"""Build a model of the null hypothesis.
"""
group1, group2 = self.data
self.n, self.m = len(group1), len(group2)
self.pool = numpy.hstack((group1, group2))
def RunModel(self):
"""Run the model of the null hypothesis.
returns: simulated data
"""
numpy.random.shuffle(self.pool)
data = self.pool[:self.n], self.pool[self.n:]
return data

Now we can run the test by instantiating a DiffMeansPermute object:

```
In [ ]:
```data = (firsts.prglngth, others.prglngth)
ht = DiffMeansPermute(data)
p_value = ht.PValue(iters=1000)
print('\nmeans permute pregnancy length')
print('p-value =', p_value)
print('actual =', ht.actual)
print('ts max =', ht.MaxTestStat())

And we can plot the sampling distribution of the test statistic under the null hypothesis.

```
In [ ]:
```ht.PlotHist()

```
In [ ]:
```# Solution goes here
class DiffStdPermute(DiffMeansPermute):
"""Tests a difference in means by permutation."""
def TestStatistic(self, data):
"""Computes the test statistic.
data: data in whatever form is relevant
"""
group1, group2 = data
test_stat = abs(group1.std() - group2.std())
return test_stat

Here's the code to test your solution to the previous exercise.

```
In [ ]:
```data = (firsts.prglngth, others.prglngth)
ht = DiffStdPermute(data)
p_value = ht.PValue(iters=1000)
print('\nstd permute pregnancy length')
print('p-value =', p_value)
print('actual =', ht.actual)
print('ts max =', ht.MaxTestStat())

```
In [ ]:
```data = (firsts.totalwgt_lb.dropna(), others.totalwgt_lb.dropna())
ht = DiffMeansPermute(data)
p_value = ht.PValue(iters=1000)
print('\nmeans permute birthweight')
print('p-value =', p_value)
print('actual =', ht.actual)
print('ts max =', ht.MaxTestStat())

In this case, after 1000 attempts, we never see a sample difference as big as the observed difference, so we conclude that the apparent effect is unlikely under the null hypothesis. Under normal circumstances, we can also make the inference that the apparent effect is unlikely to be caused by random sampling.

One final note: in this case I would report that the p-value is less than 1/1000 or less than 0.001. I would not report p=0, because the apparent effect is not impossible under the null hypothesis; just unlikely.

In this section, we'll explore the dangers of p-hacking by running multiple tests until we find one that's statistically significant.

Suppose we want to compare IQs for two groups of people. And suppose that, in fact, the two groups are statistically identical; that is, their IQs are drawn from a normal distribution with mean 100 and standard deviation 15.

I'll use `numpy.random.normal`

to generate fake data I might get from running such an experiment:

```
In [ ]:
```group1 = numpy.random.normal(100, 15, size=100)
group2 = numpy.random.normal(100, 15, size=100)

```
In [ ]:
```group1.mean(), group2.mean()

```
In [ ]:
```data = (group1, group2)
ht = DiffMeansPermute(data)
p_value = ht.PValue(iters=1000)
p_value

```
In [ ]:
```if p_value < 0.05:
print('Congratulations! Publish it!')
else:
print('Too bad! Please try again.')

You can probably see where this is going. If we play this game over and over (or if many researchers play it in parallel), the false positive rate can be as high as 100%.

To see this more clearly, let's simulate 100 researchers playing this game. I'll take the code we have so far and wrap it in a function:

```
In [ ]:
```def run_a_test(sample_size=100):
"""Generate random data and run a hypothesis test on it.
sample_size: integer
returns: p-value
"""
group1 = numpy.random.normal(100, 15, size=sample_size)
group2 = numpy.random.normal(100, 15, size=sample_size)
data = (group1, group2)
ht = DiffMeansPermute(data)
p_value = ht.PValue(iters=200)
return p_value

Now let's run that function 100 times and save the p-values.

```
In [ ]:
```num_experiments = 100
p_values = numpy.array([run_a_test() for i in range(num_experiments)])
sum(p_values < 0.05)

```
In [ ]:
```bins = numpy.linspace(0, 1, 21)
bins

```
In [ ]:
```pyplot.hist(p_values, bins, color=COLOR5)
pyplot.axvline(0.05, linewidth=3, color='0.8')
pyplot.xlabel('p-value')
pyplot.ylabel('count')
None

The distribution of p-values is uniform from 0 to 1. So it falls below 5% about 5% of the time.

**Exercise:** If the threshold for statistical signficance is 5%, the probability of a false positive is 5%. You might hope that things would get better with larger sample sizes, but they don't. Run this experiment again with a larger sample size, and see for yourself.

In the previous section, we computed the false positive rate, which is the probability of seeing a "statistically significant" result, even if there is no statistical difference between groups.

Now let's ask the complementary question: if there really is a difference between groups, what is the chance of seeing a "statistically significant" result?

The answer to this question is called the "power" of the test. It depends on the sample size (unlike the false positive rate), and it also depends on how big the actual difference is.

We can estimate the power of a test by running simulations similar to the ones in the previous section. Here's a version of `run_a_test`

that takes the actual difference between groups as a parameter:

```
In [ ]:
```def run_a_test2(actual_diff, sample_size=100):
"""Generate random data and run a hypothesis test on it.
actual_diff: The actual difference between groups.
sample_size: integer
returns: p-value
"""
group1 = numpy.random.normal(100, 15,
size=sample_size)
group2 = numpy.random.normal(100 + actual_diff, 15,
size=sample_size)
data = (group1, group2)
ht = DiffMeansPermute(data)
p_value = ht.PValue(iters=200)
return p_value

Now let's run it 100 times with an actual difference of 5:

```
In [ ]:
```p_values = numpy.array([run_a_test2(5) for i in range(100)])
sum(p_values < 0.05)

With sample size 100 and an actual difference of 5, the power of the test is approximately 65%. That means if we ran this hypothetical experiment 100 times, we'd expect a statistically significant result about 65 times.

That's pretty good, but it also means we would NOT get a statistically significant result about 35 times, which is a lot.

Again, let's look at the distribution of p-values:

```
In [ ]:
```pyplot.hist(p_values, bins, color=COLOR5)
pyplot.axvline(0.05, linewidth=3, color='0.8')
pyplot.xlabel('p-value')
pyplot.ylabel('count')
None

Here's the point of this example: if you get a negative result (no statistical significance), that is not always strong evidence that there is no difference between the groups. It is also possible that the power of the test was too low; that is, that it was unlikely to produce a positive result, even if there is a difference between the groups.

**Exercise:** Assuming that the actual difference between the groups is 5, what sample size is needed to get the power of the test up to 80%? What if the actual difference is 2, what sample size do we need to get to 80%?

```
In [ ]:
```